Yield and Quality Characteristics of Lettuce Lines Developed by Mutation Breeding

Şule Kökpınar; Kadriye Yaprak Kantoglu; Şeküre Şebnem Ellialtıoğlu

doi:10.29133/yyutbd.1666194

Yield and Quality Characteristics of Lettuce Lines Developed by Mutation Breeding

Abstract

In this study, we aimed to create genetic variation through physical mutagen application to lettuce (Lactuca sativa var. longifolia), a vegetable species whose leaves are consumed fresh, to obtain new varieties rich in nutritional content that can create market demand from this gene pool. Cervantes and Escule lettuce seeds were irradiated with effective mutation doses determined specific to the variety using a Cobalt-60 gamma ray source. Selection and inbreeding studies were performed for 4 generations, and 36 mutant lines were selected from the lines at the M4 stage to be used in subsequent experiments. Height, diameter, weight, leaf color, water-soluble dry matter (WSDM), chlorophyll, total carotenoid, vitamin C, lutein and total phenolic content of lettuce heads were examined in 36 mutant lines selected and in 4 commercial control varieties. The differences between the data obtained were statistically significant. Mutant lines numbered 62, 66, 71, 72, 74, 77, 84, and 100 which were found to be superior in terms of both morphological characteristics and nutritional content, were selected for inclusion in yield trials as a variety of candidates. These findings represent a significant step in increasing genetic diversity in agricultural production and developing lettuce varieties with higher yield and nutritional value.

Keywords

Supporting Institution

This research was funded by the Republic of Turkey Ministry of Agriculture and Forestry, General Directorate of Agricultural Research And Policies.

Ethical Statement

Ethical approval is not required for this study because no harm was done to nature and the environment.

References

Aeamovie-Djokovie, G., Pavlovie, R., Mladenovie, J., & Djurie, M. (2011). Vitamin C content of different types of lettuce varieties. Acta Agriculturae, 32, 83-89.
Afton, W. D. (2018). Evaluation of growth characteristics, yield, marketability, and nitrate levels of lettuce (Lactuca sativa L.) cultivars produced in South Louisiana. (Master’s thesis), Louisiana State University, Baton Rouge, United States.
Anne, S., & Lim, J. H. (2020). Mutation breeding using gamma irradiation in the development of ornamental plants: A review. Flower Research Journal, 28(3), 102-115. doi:https://doi.org/ 10.11623/frj.2020.28.3.01.
Anusiya, M., & Sivachandiran, S. (2019). Effect of different shade levels on growth and yield performance of lettuce. International Journal of Forestry and Horticulture, 5(3), 1-4. doi:https://doi.org/10.20431/2454-9487.0503001.
Atak, E., & Uslu, E. (2018). Phenolic compounds, extraction methods and analytical techniques. Journal of Technical Sciences, 3(27), 39-48.
Bado, S., Forster, B. P., & Nielen, S. (2015). Plant mutation breeding: Current progress and future assessment. Plant Breeding Reviews, 39, 23-88. doi:https://doi.org/10.1002/978111910 7743.ch2.
Baslam, M., Morales, F., Garmendiac, I., & Goicoecheaa, N. (2013). Nutritional quality of outer and inner leaves of green and red pigmented lettuces (Lactuca sativa L.) consumed as salads. Scientia Horticulturae, 151, 103-111. doi:https://doi.org/10.1016/j.scienta.2012.12.023.
Beşirli, G., Göçmen, M., Yanmaz, R., & Kantoğlu, K. Y. (2008). Diversity of some garlic (Allium sativum L.) genotypes and mutants by RAPD markers. In G. Çağlar, K. Abak, & I. E. Akinci (Eds.), Proceedings of 4th National Vegetable Culture Symposium Book (pp. 49-54).

Brazaitytė, A., Vaštakaitė-Kairienė, V., Sutulienė, R., Rasiukevičiūtė, N., Viršilė, A., Miliauskienė, J., Laužikė, K., Valiuškaitė, A., Dėnė, L., Chrapačienė, S., Kupčinskienė, A., & Samuolienė, G. (2022). Phenolic compounds content evaluation of lettuce grown under short-term preharvest daytime or nighttime supplemental LEDs. Plants, 11(9), 1123. doi:https://doi.org/10.3390/plants11091123.
Caldwell, C. R., & Britz, S. J. (2006). Effect of supplemental ultraviolet radiation on the carotenoid and chlorophyll composition of greenhouse-grown leaf lettuce (Lactuca sativa L.) cultivars. Journal of Food Composition and Analysis, 19, 637–644. doi:https://doi.org/10.1016/j.jfca.2005.12.016.
Chakraborty, S., Mahapatra, S., Hooi, A., Ali, N., & Satdive, R. (2023). Determination of median lethal (LD50) and growth reduction (GR50) dose of gamma irradiation for induced mutation in wheat. Agriculture, Agribusiness and Biotechnology, 66, e23220294. doi:https://doi.org/10.1590/1678-4324-2023220294.
Cruz, R., Bastista, P., Cunha, S., Pereira, J., & Casal, S. (2012). Carotenoids of lettuce (Lactuca sativa L.) grown on soil enriched with spent coffee grounds. Molecules, 17, 1535-1547. doi:https://doi.org/ 10.3390/molecules17021535.
Çivit, B. (2010). Effects of some natural substances (gypsum, zeolite, and leonardite) on yield and growth of lettuce (Lactuca sativa L. var. longifolia). (Master’s thesis), Kahramanmaraş Sütçü İmam University, Kahramanmaraş, Türkiye.
Demirci, G. (2012). Effects of grape marc and different mineral fertilizers on yield and tipburn in lettuce. (Master’s thesis), T.R. Namık Kemal University, Tekirdağ, Türkiye.
De Vries, I. M. (1997). Origin and domestication of Lactuca sativa L. Genetic Resources and Crop Evolution, 44, 165–174.
Duman, S. (2007). Effects of different planting dates on plant growth and yield of lettuce grown in high tunnels during the autumn season under Erzurum conditions. (Master’s thesis), Atatürk University, Erzurum, Türkiye.
Ellialtıoğlu, Ş. Ş., Yanmaz, R., & Kurtar, E. S. (2023). Lettuce and salad breeding. In: Vegetable Breeding Volume VI – Legumes, Asteraceae (pp. 372–479). BISAB Publications, Gece Kitaplığı Publishing House, Ankara.
Erduran, H. E. (2019). Investigation of the effects of foliar application of humic acid and 20-20-20 fertilizer on yield characteristics and harvest time of lettuce (Lactuca sativa L.). (Master’s thesis), Celal Bayar University, Manisa, Türkiye.
FAO, (2024). FAO database. http://faostat.fao.org. Access date: 18.12.2024.
Ferruzzi, M. G., Sander, L. C., Rock, C. L., & Schwartza, S. J. (1998). Carotenoid determination in biological microsamples using liquid chromatography with a coulometric electrochemical array detector. Analytical Biochemistry, 26(1), 74–81. doi:https://doi.org/10.1006/abio.1997.2484.
Giovanelli, G., Zanoni, B., Lavelli, V., & Nani, R. (2002). Water sorption, drying, and antioxidant properties of dried tomato products. Journal of Food Engineering, 52, 135–141. doi:https://doi.org/10.1016/S0260-8774(01)00095-4.
Gurdon, C., Poulev, A., & Armas, I. (2019). Genetic and phytochemical characterization of lettuce flavonoid biosynthesis mutants. Scientific Reports, 9, 3305. doi:https://doi.org/10.1038/s41598-019-39287-y
Gün, A. (2019). Effects of organic fertilizers on yield and quality in lettuce (Lactuca sativa L. var. crispa). (Master’s thesis), Ordu University, Ordu, Türkiye.
Günay, A. (1995). Special vegetable cultivation (Volume II). Çağ Printing House.
Harbart, V., Frede, K., Fitzner, M., & Baldermann, S. (2023). Regulation of carotenoid and flavonoid biosynthetic pathways in Lactuca sativa var. capitate L. in protected cultivation. Frontiers in Plant Science, 14, 1124750. doi:https://doi.org/10.3389/fpls.2023.1124750
Hassan, N., Mekkawy, S. A., Mahdy, M., Salem, K. F. M., & Tawfik, E. (2021). Recent molecular and breeding strategies in lettuce (Lactuca spp.). Genetic Resources and Crop Evolution, 68, 3055–3079. https://doi.org/10.1007/s10722-021-01246-w.
Hee-Ju, J., Young-Cho, S., Yeon-Song, H., Ju, S., Han-Yoon, Y., & Jin-Yeum, K. (2021). Growth and carotenoid contents of intercropped vegetables in building-integrated urban agriculture. Hindawi Journal of Food Quality, (9 pages). doi:https://doi.org/10.1155/2021/1159567.
Hughes, D. A. (2000). Dietary antioxidants and human immune function. Nutrition Bulletin, 25, 35-41.
Jan, S., Parween, T., Siddiqi, T. O., & Mahmooduzzafar, X. (2012). Effect of gamma radiation on morphological, biochemical, and physiological aspects of plants and plant products. Environmental Reviews, 20(1), 17-39. doi:https://doi.org/10.1139/a11-021
Kantoğlu, Y., Tepe, A., Kunter, B., Fırat, A. F., & Peşkircioğlu, H. (2014). Vegetable crops breeding by induced mutation and a practical case study of Capsicum annuum L. In N. B. Tomlekova, M. I. Kozgar, & M. R. Wani (Eds.), Mutagenesis: Exploring genetic diversity of crops (pp. 41-55). Wageningen Academic Publishers.
Kantoğlu, K. Y. (2022). Effects of gamma irradiation on seed germination in watermelon (Citrullus lanatus (Thunb.) Matsum & Nakai) and determination of effective mutation dose. Alatarım, 21(1), 10-17.
Kantoğlu, K. Y., İç, E., Özmen, D., Bulut, F. Ş., Ergun, E., Kantoğlu, Ö., & Özçoban, M. (2023). Improvement in the phytonutrient capacities of tomato by radiation-induced mutation. Frontiers in Horticulture, 2, 1190145.
Kayan, N., & Eser, D. (2001). Effects of different doses of gamma radiation applied to faba bean (Vicia faba L.) seeds on yield and yield components in the M3 generation. Journal of Field Crops Central Research Institute, 10, 43-49.
Kaymak, N. (2007). Determination of the critical period for weed control in lettuce (Lactuca sativa L.). (Master’s thesis), Atatürk University, Erzurum, Türkiye.
Kim, M. J., Moon, Y., Kopsell, D. A., Park, S., Tou, J. C., & Waterland, N. L. (2016). Nutritional value of crisphead ‘Iceberg’ and ‘Romaine’ lettuces (Lactuca sativa L.). Journal of Agricultural Science, 8(11), 1-1. doi:https://doi.org/10.5539/jas.v8n11p1.
Kimani, P.M. (1989). Improvement of food beans (Phaselous vulgaris L.) through mutation breeding. P. B. A., 59(2), 180.
Kovácsné Madar, Á., & Takácsné Hájos, M. (2022). Agronomic evaluation of different lettuce (Lactuca sativa L.) varieties under unheated plastic tunnel. International Journal of Horticultural Science, 28, 50-56. doi:https://doi.org/10.31421/ijhs/28/2022/10314
Kökpınar, Ş. (2022). Lettuce and salad production in Türkiye. Turkish Agriculture and Forestry Journal, 270, 48-53.
Kökpınar, Ş., Kantoğlu, K. Y., & Ellialtıoğlu, Ş. Ş. (2024). Morphological effects of mutation breeding in lettuce (Lactuca sativa var. longifolia). Bahçe, 5 (1), 364-373.
Köse, A., Koşar, F. Ç., & Bilir, Ö. (2021). Agronomic performance of some safflower (Carthamus tinctorius L.) lines developed through mutation breeding. Turkish Journal of Agriculture and Natural Sciences, 8(2), 262-273. doi:https://doi.org/10.30910/turkjans.685982.
Kreutz, G. F., Sandoya, G. V., England, G. K., & Mussoline, W. (2020). Exploring the potential of lettuce (Lactuca sativa L.) as an early crop in Florida’s sandy soils. HortScience, 55(12), 1-12. doi:https://doi.org/10.21273/HORTSCI15420-20.
Llorach, R., Martínez-Sánchez, A., Tomás-Barberán, F. A., Gil, M. I., & Ferreres, F. (2008). Characterisation of polyphenols and antioxidant properties of five lettuce varieties and escarole. Food Chemistry, 108(3), 1028-1038. doi:https://doi.org/10.1016/j.foodchem.2007.11.032.
Long, H., Miller, S. F., Williams, E., & Lynch, M. (2018). Specificity of the DNA mismatch repair system (MMR) and mutagenesis bias in bacteria. Molecular Biology and Evolution, 35(10), 2414-2421. doi:10.1093/molbev/msy134.
López, A., Javier, G. A., Fenoll, J., Hellin, P., & Flores, P. (2014). Chemical composition and antioxidant capacity of lettuce: Comparative study of regular-sized (Romaine) and baby-sized (Little Gem and Mini Romaine) types. Journal of Food Composition and Analysis, 33(1), 39-48.
López, U. P., Apodaca, J. M., Rueda, A. M., & Petite, A. M. (2015). Interacting effects of high light and elevated CO₂ on the nutraceutical quality of two differently pigmented Lactuca sativa cultivars (Blonde of Paris Batavia and Oak Leaf). Scientia Horticulturae, 191(6), 38-48. doi:https://doi.org/10.1016/j.scienta.2015.04.030.
Mohamoud, S. S. (2019). Effects of different water stress conditions on yield and quality of some leaf (Lactuca sativa var. crispa) and romaine (Lactuca sativa var. longifolia) lettuce cultivars. (Master’s thesis), Akdeniz University, Antalya, Türkiye.
Mahmoudi, H., Huang, J., Gruber, M. Y., Kaddour, R. Y. M., Lachaaˆl, M., Ouerghi, Z., & Hannoufa, A. J. (2010). The impact of genotype and salinity on physiological function, secondary metabolite accumulation, and antioxidative responses in lettuce. Journal of Agricultural and Food Chemistry, 58, 5122-5130. doi:https://doi.org/10.1021/jf904274v.
Martínez-Sánchez, A., Tudela, J. A., Consuelo Luna, C., Allende, A., & Gil, M. I. (2011). Low oxygen levels and light exposure affect quality of fresh-cut Romaine lettuce. Postharvest Biology and Technology, 59(1), 34-42. doi:https://doi.org/10.1016/j.postharvbio.2010.07.005.
Masuda, M., Agong, S., Tanaka, A., Shikazono, N., & Hase, Y. (2004). Mutation spectrum of tomato seed induced by radiation with helium ion beams and coal. Acta Horticulturae, 637, 257-262. doi:https://doi.org/10.17660/ActaHortic.2004.637.31.
Matysiak, B., Ropelewska, E., Wrzodak, A., Kowalski, A., & Kaniszewski, S. (2022). Yield and quality of romaine lettuce at different daily light integral in an indoor controlled environment. Agronomy, 12(5), 1026. doi:https://doi.org/10.3390/agronomy12051026.
Mou, B. (2005). Genetic variation of beta-carotene and lutein contents in lettuce. Journal of the American Society for Horticultural Science, 130(6), 870-876. doi:https://doi.org/10.21273/JASHS.130.6.870.
Mou, B. (2008). Lettuce. In J. Prohens, F. Nuez, & M. J. Carena (Eds.), Vegetables I: Asteraceae, Brassicaceae, Chenopodicaceae, and Cucurbitaceae (pp. 75-119). Universidad Politecnica de Valencia.
Mou, B. (2011). Mutations in lettuce improvement. International Journal of Plant Genomics, 3, 723518. doi:https://doi.org/10.1155/2011/723518
Mousavizadeh, S. J., & Sedaghathoor, S. (2011). Peroxidase activity in response to applying natural antioxidant of essential oils in some leafy vegetables. Australian Journal of Crop Science, 5(4), 494-499.
MVD, (2024). International Atomic Energy Agency (IAEA). https://nucleus.iaea.org/sites/mvd/SitePages/Home.aspx. Access date: 18.12.2024.
Nicolle, C., Carnat, A., Fraisse, D., Lamaison, J. J., Rock, E., & Michel, H. (2004). Characterisation and variation of antioxidant micronutrients in lettuce (Lactuca sativa folium). Journal of the Science of Food and Agriculture, 84, 2061-2069. doi:https://doi.org/10.1002/jsfa.1916.
Noumedem, J. A. K., Djeussi, D. E., Hritcu, L., Mihasan, M., & Kuete, V. (2017). Lactuca sativa. In Medicinal Spices and Vegetables from Africa (pp. 437-449). Academic Press.
Oh, M. M., Carey, E. E., & Rajashekar, C. B. (2011). Antioxidant phytochemicals in lettuce grown in high tunnels and open field. Horticulture, Environment, and Biotechnology, 52(2), 133-139.
Owen, W. G., & Lopez, R. G. (2015). End-of-production supplemental lighting with red and blue light-emitting diodes (LEDs) influences red pigmentation of four lettuce varieties. HortScience, 50(5), 676-684. doi:https://doi.org/10.21273/HORTSCI.50.5.676.
Ozgen, S., & Sekerci, S. (2011). Effect of leaf position on the distribution of phytochemicals and antioxidant capacity among green and red lettuce cultivars. Spanish Journal of Agricultural Research, 9(3), 801-809. doi:https://doi.org/10.5424/sjar/20110903-472-10.
Özdamar, K. (2013). Statistical data analysis using software packages. 1 (1st ed.). Türkiye.
Pino-Nunes, L. E., de O. Figueira, A. V., & Tulmann Neto, A. (2009). Induced mutagenesis and natural genetic variation in tomato ‘Micro-Tom’. Proceedings of the ISHS Symposium on Tomato in the Tropics, Acta Horticulturae, 821, 63-72. https://doi.org/10.17660/ActaHortic.2009.821.5.
Sağel, Z., Peşkircioğlu, H., Tutluer, İ., Uslu, N., Şenay, A., Taner, K. Y., Kunter, B., Şekerci, S., & Yalçın, S. (2002). Mutation and tissue culture techniques in plant breeding. III National Mutation Course, Lecture Notes, TAEK, ANTHAM, Nuclear Agriculture Department, Ankara, Türkiye.
Sarıçam, Ş., Kantoğlu, K. Y., & Ellialtıoğlu, Ş. Ş. (2017). Determination of effective mutagen dose for lettuce (Lactuca sativa var. longifolia cv. Cervantes) seeds. Eurasian Journal of Agricultural Research, 1(2), 96-101.
Sarıçam, Ş., Kantoğlu, K. Y., & Ellialtıoğlu, Ş. Ş. (2018). Determination of effective mutation dose for coated and uncoated lettuce (Lactuca sativa var. longifolia cv. Cervantes) seeds. International Agriculture, Environment and Health Congress / 26-28 October 2018, Aydın-Türkiye, s: 1450-1459.
Seçkin, S. D. (2019). Effects of different LED lights and nitrogen applications on the growth and leaf nitrate concentration of lettuce plants. (Master’s thesis), Gaziosmanpaşa University, Tokat, Türkiye.
Serafini, M., Bugianesi, R., Salucci, M., Azzini, E., Raguzzini, A., & Maiani, G. (2002). Effect of acute ingestion of fresh and stored lettuce (Lactuca sativa) on plasma total antioxidant capacity and antioxidant levels in human subjects. British Journal of Nutrition, 88, 615-623. https://doi.org/10.1079/BJN2002722.
Serea, C., Barna, O., Manley, M., & Kidd, M. (2014). Effect of storage temperature on the ascorbic acid content, total phenolic content, and antioxidant activity in lettuce (Lactuca sativa L.). The Journal of Animal & Plant Sciences, 24(4), 1173-1177.
Siomos, A. S., Papadopoulou, P. P., Niklis, N. D., & Dogras, C. C. (2002). Quality of Romaine and leaf lettuce at harvest and during storage. Acta Horticulturae, 579, 641-646. doi:https://doi.org/10.17660/ActaHortic.2002.579.113.
Sirtautas, R., Samuoliene, G., Brazaityte, A., Sakalauskaite, J., Sakalauskiene, S., Virsile, A., Jankauskiene, J., Vastakaite, V., & Duchovskis, P. (2014). Impact of CO₂ on quality of baby lettuce grown under optimized light spectrum. Acta Scientiarum Polonorum, Hortorum Cultus, 13(2), 109-118.
Song, J., Huang, H., Song, S., Zhang, Y., Su, W., & Liu, H. (2020). Effects of photoperiod interacted with nutrient solution concentration on nutritional quality and antioxidant and mineral content in lettuce. Agronomy, 10(7), 920. doi:https://doi.org/10.3390/agronomy10070920.
Stojanović, M., Savić, S., Delcourt, A., Hilbert, J.-L., Hance, P., Dragišić Maksimović, J., & Maksimović, V. (2023). Phenolics and sesquiterpene lactones profile of red and green lettuce: Combined effect of cultivar, microbiological fertiliser, and season. Plants, 12(14), 2616. doi:https://doi.org/10.3390/plants12142616.
Sumanta, N., Haque, C. H., Nishika, İ., & Suprakash, R. (2014). Spectrophotometric analysis of chlorophylls and carotenoids from commonly grown fern species by using various extracting solvents. Research Journal of Chemical Sciences, 4(9), 63-69.
Suprasanna, P., Mirajkar, S. J., & Bhagwat, S. G. (2015). Induced mutations and crop improvement. In I. N. B. Bahadur (Ed.), Plant Biology and Biotechnology (pp. 593-617). New Delhi.
Şen, F., Kınayteksür, P., Okşar, R. E., Güleş, A., & Kaygısız Aşçıoğul, T. (2016). Effect of beneficial microorganism application on lettuce yield and quality characteristics. Adnan Menderes University, Faculty of Agriculture Journal, 13(1), 35-40.
Taner, Y., Beşirli, G., Kunter, B., & Yanmaz, R. (2004). Determination of the effective mutation dose for radiation-induced mutation breeding in garlic (Allium sativum L.). Bahçe, 33(1-2), 95-99.
Teng, Z., Luo, Y., Bornhorst, E. R., Zhou, B., Simko, I., & Trouth, F. (2019). Identification of Romaine lettuce (Lactuca sativa var. longifolia) cultivars with reduced browning discoloration for fresh-cut processing. Postharvest Biology and Technology, 156, 110931. doi:https://doi.org/10.1016/j.postharvbio.2019.110931.
Tepe, A., Fırat, A. F., Taner, Y. K., Kunter, B., Peşkircioğlu, H., & Ekiz, H. (2003). Determination of the effective mutation dose for mutation breeding in greenhouse cultivar Demre 8 pepper. Türkiye IV National Horticultural Crops Congress, 08–12 September, Antalya, Türkiye.
TUIK, (2024). Crop production statistics. https://data.tuik.gov.tr/Kategori/GetKategori?p=Tarim-111. Access date: 18.12.2024.
Tüzel, Y., Öztekin, G. B., Duyar, H., Eşiyok, D., Gürbüz Kılıç, Ö., Anaç, D., & Kayıkçıoğlu, H. H. (2011). Effects of Agryl row cover and selected fertilizers on yield, quality, leaf nutrient content, and soil fertility in organic lettuce cultivation. Journal of Agricultural Sciences, 17, 190-203. doi:https://doi.org/10.1501/Tarimbil_0000001171.
Van Harten, A. M. (1998). Mutation breeding: Theory and practical applications. Cambridge University Press, United Kingdom.
Vargas-Arcila, M., Cartagena-Valenzuela, J. R., Franco, G., Correa-Londoño, G. A., Quintero-Vásquez, L. M., & Gaviria-Montoya, C. A. (2017). Changes in the physico-chemical properties of four lettuce (Lactuca sativa L.) varieties during storage. Corpoica Ciencia y Tecnología Agropecuaria, 18(2), 257-273.
Witkowska, L. I. M., & Woltering, E. J. (2014). Storage of intact heads prior to processing limits the shelf-life of fresh-cut Lactuca sativa. Postharvest Biology and Technology, 91, 25-30. doi:https://doi.org/10.1016/j.postharvbio.2013.12.011.
Yali, W., & Mitiku, T. (2022). Mutation breeding and its importance in modern plant breeding. Journal of Plant Sciences, 10(2), 64-70. doi:https://doi.org/10.11648/j.jps.20221002.13.

Details

Primary Language

English

Subjects

Crop and Pasture Breeding

Journal Section

Research Article

Authors

Şule Kökpınar ^*
0000-0002-6481-5618
Türkiye

Kadriye Yaprak Kantoglu
0000-0002-7247-9116
Türkiye

Şeküre Şebnem Ellialtıoğlu
0000-0002-3851-466X
Türkiye

Publication Date

December 25, 2025

Submission Date

March 27, 2025

Acceptance Date

July 1, 2025

Published in Issue

Year 2025 Volume: 35 Number: 4

DOI

https://doi.org/10.29133/yyutbd.1666194

IZ

https://izlik.org/JA55DU53ZU

Cite

RIS / Bibtex

APA

Kökpınar, Ş., Kantoglu, K. Y., & Ellialtıoğlu, Ş. Ş. (2025). Yield and Quality Characteristics of Lettuce Lines Developed by Mutation Breeding. Yuzuncu Yıl University Journal of Agricultural Sciences, 35(4), 644-658. https://doi.org/10.29133/yyutbd.1666194

AMA

1.Kökpınar Ş, Kantoglu KY, Ellialtıoğlu ŞŞ. Yield and Quality Characteristics of Lettuce Lines Developed by Mutation Breeding. YYU J AGR SCI. 2025;35(4):644-658. doi:10.29133/yyutbd.1666194

Chicago

Kökpınar, Şule, Kadriye Yaprak Kantoglu, and Şeküre Şebnem Ellialtıoğlu. 2025. “Yield and Quality Characteristics of Lettuce Lines Developed by Mutation Breeding”. Yuzuncu Yıl University Journal of Agricultural Sciences 35 (4): 644-58. https://doi.org/10.29133/yyutbd.1666194.

EndNote

Kökpınar Ş, Kantoglu KY, Ellialtıoğlu ŞŞ (December 1, 2025) Yield and Quality Characteristics of Lettuce Lines Developed by Mutation Breeding. Yuzuncu Yıl University Journal of Agricultural Sciences 35 4 644–658.

IEEE

[1]Ş. Kökpınar, K. Y. Kantoglu, and Ş. Ş. Ellialtıoğlu, “Yield and Quality Characteristics of Lettuce Lines Developed by Mutation Breeding”, YYU J AGR SCI, vol. 35, no. 4, pp. 644–658, Dec. 2025, doi: 10.29133/yyutbd.1666194.

ISNAD

Kökpınar, Şule - Kantoglu, Kadriye Yaprak - Ellialtıoğlu, Şeküre Şebnem. “Yield and Quality Characteristics of Lettuce Lines Developed by Mutation Breeding”. Yuzuncu Yıl University Journal of Agricultural Sciences 35/4 (December 1, 2025): 644-658. https://doi.org/10.29133/yyutbd.1666194.

JAMA

1.Kökpınar Ş, Kantoglu KY, Ellialtıoğlu ŞŞ. Yield and Quality Characteristics of Lettuce Lines Developed by Mutation Breeding. YYU J AGR SCI. 2025;35:644–658.

MLA

Kökpınar, Şule, et al. “Yield and Quality Characteristics of Lettuce Lines Developed by Mutation Breeding”. Yuzuncu Yıl University Journal of Agricultural Sciences, vol. 35, no. 4, Dec. 2025, pp. 644-58, doi:10.29133/yyutbd.1666194.

Vancouver

1.Şule Kökpınar, Kadriye Yaprak Kantoglu, Şeküre Şebnem Ellialtıoğlu. Yield and Quality Characteristics of Lettuce Lines Developed by Mutation Breeding. YYU J AGR SCI. 2025 Dec. 1;35(4):644-58. doi:10.29133/yyutbd.1666194