Phenolic Compound Content of Stems, Leaves, and Flowers of Prunus avium L. Varieties in Çankırı (Eldivan) Region

Dilek Akın; Osman Şentürk

doi:10.29133/yyutbd.1711437

Phenolic Compound Content of Stems, Leaves, and Flowers of Prunus avium L. Varieties in Çankırı (Eldivan) Region

Abstract

In this study, phenolic compounds of methanol extracts obtained from stems (fruit pedicels), leaves, and flowers of two different varieties (0900 Ziraat and Regina) of cherry (Prunus avium L.) in Çankırı (Eldivan) region were investigated. Component determination was performed using an LC-MS/MS instrument. The data obtained revealed that different plant parts of both varieties differed in phenolic content. The major components identified were quinic acid, epicatechin, catechin, chlorogenic acid, and rutin. Accordingly, the major components were found to be higher in 0900 Ziraat extracts rather than Regina extracts. The highest values of the major components, catechin (44222 ± 480.74 µg g-1, 41288 ± 176.38 µg g-1) and epicatechin (9577.78 ± 38.49 µg g-1, 9377 ± 101.83 µg g-1) were determined in the stems of 0900 Ziraat and Regina varieties, respectively. The highest concentrations of rutin, chlorogenic acid, and quinic acid were obtained from the leaf extracts of both varieties. The highest phenolic content was observed in stem extracts of both varieties, followed by leaf and flower extracts. This result indicates that stem tissue has a more active metabolic profile for phenolic accumulation. These findings suggest that the stem (fruit pedicel), leaf, and flower tissues of cherry may be relatively rich in phenolic compounds and provide a baseline dataset supporting their potential evaluation as natural sources of antioxidants.

Keywords

Supporting Institution

TÜBİTAK

Project Number

1919B012322159

Ethical Statement

Ethical approval is not required for this study because it does not involve human participants, animal subjects or any procedures requiring ethical clearance.

Thanks

This study was supported by TÜBİTAK within the scope of TÜBİTAK 2209-A 2023/2 University Students Research Projects Support Program (Project Number: 1919B012322159).

References

Ademovic, Z., Hodžić, S., Halilić-Zahirović, Z., Husejnagić, D., Džananović, J., Šarić-Kundalić, B., & Suljagić, J. (2017). Phenolic compounds, antioxidant and antimicrobial properties of the wild cherry (Prunus avium L.) stem. Acta Periodica Technologica, (48), 1-13. https://doi.org/10.2298/APT1748001A
Aglar, E., Saracoglu, O., Karakaya, O., Ozturk, B., & Gun, S. (2019). The relationship between fruit color and fruit quality of sweet cherry (Prunus avium L. cv.‘0900 Ziraat’). Turkish Journal of Food and Agriculture Sciences, 1(1), 1-5. https://izlik.org/JA35RU47UK
Aglar, E., Saracoglu, O., Ozturk, B., Karakaya, O., & Ateş, U. (2023). The influence of fruit size on quality attributes and bioactive compounds of sweet cherry fruit. Erwerbs-obstbau, 65(4), 701-707. https://doi.org/10.1007/s10341-022-00726-2
Aires, A., Dias, C., Carvalho, R., & Saavedra, M. (2017). Analysis of glycosylated flavonoids extracted from sweet-cherry stems, as antibacterial agents against pathogenic Escherichia coli isolates. Acta Biochimica Polonica, 64(2), 265-271. https://doi.org/10.18388/abp.2016_1374
Ates, U., Karakaya, O., Ozturk, B., Aglar, E., & Gun, S. (2022). Training system plays a key role on fruit quality and phenolic acids of sweet cherry. Erwerbs-Obstbau, 64(2), 135-141. https://doi.org/10.1007/s10341-021-00621-2
Bastos, C., Barros, L., Dueñas, M., Calhelha, R. C., Queiroz, M. J. R., Santos-Buelga, C., & Ferreira, I. C. (2015). Chemical characterisation and bioactive properties of Prunus avium L.: The widely studied fruits and the unexplored stems. Food chemistry, 173, 1045-1053. https://doi.org/10.1016/j.foodchem.2014.10.145
Bernard, G., Santos, H. A. D., Etienne, A., Samaillie, J., Neut, C., Sahpaz, S., ... & Rambaud, C. (2020). MeJA elicitation of chicory hairy roots promotes efficient increase of 3, 5-diCQA accumulation, a potent antioxidant and antibacterial molecule. Antibiotics, 9(10), 659. https://doi.org/10.3390/antibiotics9100659
Boskov, D., Milatovic, D., Rakonjac, V., Zec, G., Hudina, M., Veberic, R., & Mikulic-Petkovsek, M. (2022). The phenolic profile of sweet cherry fruits influenced by cultivar/rootstock combination. Plants, 12(1), 103. https://doi.org/10.3390/plants12010103

Bursal, E., Köksal, E., Gülçin, İ., Bilsel, G., & Gören, A. C. (2013). Antioxidant activity and polyphenol content of cherry stem (Cerasus avium L.) determined by LC–MS/MS. Food research international, 51(1), 66-74. https://doi.org/10.1016/j.foodres.2012.11.022
Çelik, Y., & Sarıaltın, H. K. (2019). Structural analysis of cherry production in Turkey. Turkish Journal of Agricultural and Natural Sciences, 6(4), 596-607. https://doi.org/10.30910/turkjans.633515
Çelik, Z., Saçtı, H., & Adanacıoğlu, H. (2019). Trends in foreign trade of cherry and Turkey’s comparative advantage. Yuzuncu Yıl University Journal of Agricultural Sciences, 29, 41-53. https://doi.org/10.29133/yyutbd.474794
De Leo, M., Iannuzzi, A. M., Germanò, M. P., D'Angelo, V., Camangi, F., Sevi, F., & Braca, A. (2021). Comparative chemical analysis of six ancient italian sweet cherry (Prunus avium L.) varieties showing antiangiogenic activity. Food chemistry, 360, 129999. https://doi.org/10.1016/j.foodchem.2021.129999
Dziadek, K., Kopeć, A., & Tabaszewska, M. (2019). Potential of sweet cherry (Prunus avium L.) by-products: bioactive compounds and antioxidant activity of leaves and petioles. European Food Research and Technology, 245(3), 763-772. https://doi.org/10.1007/s00217-018-3198-x
Dziadek, K., Kopeć, A., Piątkowska, E., Leszczyńska, T., Pisulewska, E., Witkowicz, R., ... & Francik, R. (2018). Identification of polyphenolic compounds and determination of antioxidant activity in extracts and infusions of buckwheat leaves. European Food Research and Technology, 244, 333-343. https://doi.org/10.1007/s00217-017-2959-2
Ekinci, N., & Yunusoğlu, S. V. (2023). Effects of post-harvest methyl salicylate application on the quality of "0900 Ziraat" cherry cultivar during cold storage. COMU Journal of Agriculture Faculty, 11(2), 260-267. https://doi.org/10.33202/comuagri.1390241
Ferreira-Santos, P., Zanuso, E., Genisheva, Z., Rocha, C. M., & Teixeira, J. A. (2020). Green and sustainable valorization of bioactive phenolic compounds from pinus by-products. Molecules, 25(12), 2931. https://doi.org/10.3390/molecules25122931
Food and Agriculture Organization (FAO). (2021). Sustainable Food Systems Country Report Türkiye. https://www.fao.org/common-pages/search/en/?q=cherry+t%C3%BCrkiye. Access date: 06.03.2024.
Goliáš, J., Vrchotová, N., Kožíšková, J., Kotas, P., & Tříska, J. (2018). Influence of post-harvest ripening on the levels of selected compounds in various cherry cultivars. Czech Journal of Food Sciences, 36(2), 163. https://doi.org/10.17221/149/2017-CJFS
Gonçalves, A. C., Bento, C., Silva, B. M., & Silva, L. R. (2017). Sweet cherries from Fundão possess antidiabetic potential and protect human erythrocytes against oxidative damage. Food Research International, 95, 91-100. https://doi.org/10.1016/j.foodres.2017.02.023
Hayaloglu, A. A., & Demir, N. (2016). Phenolic compounds, volatiles, and sensory characteristics of twelve sweet cherry (Prunus avium L.) cultivars grown in Turkey. Journal of food science, 81(1), C7-C18. https://doi.org/10.1111/1750-3841.13175
Jesus, F., Goncalves, A. C., Alves, G., & Silva, L. R. (2022). Health benefits of Prunus avium plant parts: An unexplored source rich in phenolic compounds. Food Reviews International, 38(sup1), 118-146. https://doi.org/10.1080/87559129.2020.1854781
Jesus, F., Gonçalves, A. C., Alves, G., & Silva, L. R. (2019). Exploring the phenolic profile, antioxidant, antidiabetic and anti-hemolytic potential of Prunus avium vegetal parts. Food Research International, 116, 600-610. https://doi.org/10.1016/j.foodres.2018.08.079
Karakaya, O., Ozturk, B., Aglar, E., & Balik, H. I. (2021). The influence of the rootstocks on biochemical and bioactive compound content of ‘0900 Ziraat’sweet cherry fruit. Erwerbs-Obstbau, 63(3), 247-253. https://doi.org/10.1007/s10341-021-00542-0
Kundu, A., & Vadassery, J. (2019). Chlorogenic acid‐mediated chemical defence of plants against insect herbivores. Plant Biology, 21(2), 185-189. https://doi.org/10.1111/plb.12947
Kutlu, T., Takim, K., Çeken, B., & Kizil, M. (2014). DNA damage protecting activity and in vitro antioxidant potential of the methanol extract of Cherry (Prunus avium L). J Med Plants Res, 8(19), 715-26. https://doi.org/10.5897/JMPR2013.5350
Küden, A., Küden, A. B., Comlekcioglu, S., Imrak, B., & Bag, M. (2022). Recent techniques and developments on cherry growing in Turkey. In A. B. Küden & A. Küden (Eds.), Prunus-recent advances (pp. 125-145). IntechOpen. https://doi.org/10.5772/intechopen.104081
Luna-Vázquez, F. J., Ibarra-Alvarado, C., Rojas-Molina, A., Rojas-Molina, J. I., Yahia, E. M., Rivera-Pastrana, D. M., ... & Zavala-Sánchez, M. Á. (2013). Nutraceutical value of black cherry Prunus serotina Ehrh. fruits: antioxidant and antihypertensive properties. Molecules, 18(12), 14597-14612. https://doi.org/10.3390/molecules181214597
Melini, V., Melini, F., Luziatelli, F., & Ruzzi, M. (2020). Functional ingredients from agri-food waste: Effect of inclusion thereof on phenolic compound content and bioaccessibility in bakery products. Antioxidants, 9(12), 1216. https://doi.org/10.3390/antiox9121216
Nawaz, M., Sun, J., Shabbir, S., Khattak, W. A., Ren, G., Nie, X., ... & Sonne, C. (2023). A review of plants strategies to resist biotic and abiotic environmental stressors. Science of the Total Environment, 900, 165832. https://doi.org/10.1016/j.scitotenv.2023.165832
Nunes, A. R., Gonçalves, A. C., Alves, G., Falcão, A., Garcia-Viguera, C., A. Moreno, D., & Silva, L. R. (2021a). Valorisation of Prunus avium L. by-products: Phenolic composition and effect on Caco-2 cells viability. Foods, 10(6), 1185. https://doi.org/10.3390/foods10061185
Nunes, A. R., Gonçalves, A. C., Falcão, A., Alves, G., & Silva, L. R. (2021b). Prunus avium L.(sweet cherry) by-products: A source of phenolic compounds with antioxidant and anti-hyperglycemic properties—A review. Applied Sciences, 11(18), 8516. https://doi.org/10.3390/app11188516
Rao, M. J., & Zheng, B. (2025). The role of polyphenols in abiotic stress tolerance and their antioxidant properties to scavenge reactive oxygen species and free radicals. Antioxidants, 14(1), 74. https://doi.org/10.3390/antiox14010074
Saavedra-Leos, M. Z., Leyva-Porras, C., Toxqui-Terán, A., & Espinosa-Solis, V. (2021). Physicochemical properties and antioxidant activity of spray-dry broccoli (Brassica oleracea var Italica) stalk and floret juice powders. Molecules, 26(7), 1973. https://doi.org/10.3390/molecules26071973
Savaş, E., & Gür, E. (2021). Effectsr of Pre-Harvest Gibberellic Acid (GA3) Applications on Fruit Quality of Early Burlat, Van, 0900 Ziraat Cherry. Lapseki Journal of Applied Research, 2(3), 122-133. https://izlik.org/JA33ZC38WP
Shahidi, F., & Yeo, J. (2016). Insoluble-bound phenolics in food. Molecules, 21(9), 1216. https://doi.org/10.3390/molecules21091216
Švarc‐Gajić, J., Cerdà, V., Clavijo, S., Suárez, R., Mašković, P., Cvetanović, A., ... & Novakov, V. (2018). Bioactive compounds of sweet and sour cherry stems obtained by subcritical water extraction. Journal of Chemical Technology & Biotechnology, 93(6), 1627-1635. https://doi.org/10.1002/jctb.5532
Syvertsen, J. P., & Garcia-Sanchez, F. (2014). Multiple abiotic stresses occurring with salinity stress in citrus. Environmental and Experimental Botany, 103, 128-137. https://doi.org/10.1016/j.envexpbot.2013.09.015
Tahir, M. S., Almezgagi, M., Zhang, Y., Bashir, A., Abdullah, H. M., Gamah, M., Wang, X., Zhu, Q., Shen, X., Ma, Q., Ali, M., Solangi, Z. A., Malik, W. S., & Zhang, W. (2021). Mechanistic new insights of flavonols on neurodegenerative diseases. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 137, 111253. https://doi.org/10.1016/j. biopha.2021.111253
Topaloğlu, E. (2015). Determination of phytochemical and antioxidant properties of water extracts of various herbal products. (Master's thesis), Pamukkale University, Institute of Science, Denizli, Türkiye.
Varzakas, T., Zakynthinos, G., & Verpoort, F. (2016). Plant food residues as a source of nutraceuticals and functional foods. Foods, 5(4), 88. https://doi.org/10.3390/foods5040088
Yüksekkaya, Ş., Başyiğit, B., Sağlam, H., Pekmez, H., Cansu, Ü., Karaaslan, A., & Karaaslan, M. (2021). Valorization of fruit processing by-products: free, esterified, and insoluble bound phytochemical extraction from cherry (Prunus avium) tissues and their biological activities. Journal of Food Measurement and Characterization, 15, 1092-1107. https://doi.org/10.1007/s11694-020-00698-5

Details

Primary Language

English

Subjects

Crop and Pasture Biochemistry and Physiology, Medicinal and Aromatic Plants

Journal Section

Research Article

Authors

Dilek Akın ^*
0000-0003-0908-9075
Türkiye

Osman Şentürk
0009-0002-5828-1688
Türkiye

Publication Date

June 1, 2026

Submission Date

June 2, 2025

Acceptance Date

March 23, 2026

Published in Issue

Year 2026 Number: 1

DOI

https://doi.org/10.29133/yyutbd.1711437

IZ

https://izlik.org/JA84MD53GG

Cite

RIS / Bibtex

APA

Akın, D., & Şentürk, O. (2026). Phenolic Compound Content of Stems, Leaves, and Flowers of Prunus avium L. Varieties in Çankırı (Eldivan) Region. Yuzuncu Yıl University Journal of Agricultural Sciences, 1, 1711437. https://doi.org/10.29133/yyutbd.1711437

AMA

1.Akın D, Şentürk O. Phenolic Compound Content of Stems, Leaves, and Flowers of Prunus avium L. Varieties in Çankırı (Eldivan) Region. YYU J AGR SCI. 2026;(1):1711437. doi:10.29133/yyutbd.1711437

Chicago

Akın, Dilek, and Osman Şentürk. 2026. “Phenolic Compound Content of Stems, Leaves, and Flowers of Prunus Avium L. Varieties in Çankırı (Eldivan) Region”. Yuzuncu Yıl University Journal of Agricultural Sciences, no. 1: 1711437. https://doi.org/10.29133/yyutbd.1711437.

EndNote

Akın D, Şentürk O (June 1, 2026) Phenolic Compound Content of Stems, Leaves, and Flowers of Prunus avium L. Varieties in Çankırı (Eldivan) Region. Yuzuncu Yıl University Journal of Agricultural Sciences 1 1711437.

IEEE

[1]D. Akın and O. Şentürk, “Phenolic Compound Content of Stems, Leaves, and Flowers of Prunus avium L. Varieties in Çankırı (Eldivan) Region”, YYU J AGR SCI, no. 1, p. 1711437, June 2026, doi: 10.29133/yyutbd.1711437.

ISNAD

Akın, Dilek - Şentürk, Osman. “Phenolic Compound Content of Stems, Leaves, and Flowers of Prunus Avium L. Varieties in Çankırı (Eldivan) Region”. Yuzuncu Yıl University Journal of Agricultural Sciences. 1 (June 1, 2026): 1711437. https://doi.org/10.29133/yyutbd.1711437.

JAMA

1.Akın D, Şentürk O. Phenolic Compound Content of Stems, Leaves, and Flowers of Prunus avium L. Varieties in Çankırı (Eldivan) Region. YYU J AGR SCI. 2026;:1711437.

MLA

Akın, Dilek, and Osman Şentürk. “Phenolic Compound Content of Stems, Leaves, and Flowers of Prunus Avium L. Varieties in Çankırı (Eldivan) Region”. Yuzuncu Yıl University Journal of Agricultural Sciences, no. 1, June 2026, p. 1711437, doi:10.29133/yyutbd.1711437.

Vancouver

1.Dilek Akın, Osman Şentürk. Phenolic Compound Content of Stems, Leaves, and Flowers of Prunus avium L. Varieties in Çankırı (Eldivan) Region. YYU J AGR SCI. 2026 Jun. 1;(1):1711437. doi:10.29133/yyutbd.1711437