Effect of Different Combinations of BAP and NAA in vitro Propagation of Wild Safflower (Carthamus persicus Wild)

Abstract

Safflower (Carthamus persicus Wild), is rich in terms of linoleic acid and oleic acid and is used to obtain low saturated fatty acid. In order to propagate safflower in vitro, shoot tip explants were cultured in MS medium containing different combinations of BAP and NAA supplemented with 3% sucrose and solidified with 0.8% agar. The results showed a significant interaction between BAP and NAA combinations for callus formation, shoot and root regeneration, number of shoots and roots per explant, shoot and root length. Maximum shoot regeneration was noted in MS medium containing 0.50 mg/L BAP + 0.25 mg/L NAA. Maximum callus formation was determined in MS medium supplemented with 1 mg/L BAP + 1 mg/L NAA and 2 mg/L BAP + 1mg/L NAA. The regenerated shoots were rooted on MS medium containing 1 mg/L IBA.

Keywords

• BAP, NAA, Safflower, Micropropagation

Yabani Aspir'in (Carthamus persicus Wild) in vitro Çoğaltımında Farklı BAP-NAA Kombinasyonlarının Etkisi

Öz

Aspir, linoleik asit ve oleik asitce zengin; yenilebilir doymamış yağ asitlerinin elde edilmesinde kullanılması nedeniyle önemli bir endüstriyel bitkidir. Bu çalışmada aspirin in vitro’ da çoğaltımı amacıyla sürgün ucu eksplantları 0.25- 0.50- 1- 2 mg/L BAP ve 0.25- 0.50-1 mg/L NAA’ nın farklı kombinasyonlarını içeren, %3 sakkaroz ilave edilmiş ve % 0.8 agar ile katılaştırılmış MS ortamında kültüre alınmıştır. Araştırma sonuçları kallus oluşumu, rejenere sürgün yüzdesi, eksplant başına sürgün sayısı, sürgün uzunluğu, eksplant başına kök sayısı ve kök uzunluğu için BAP-NAA arasındaki kombinasyoların etkili olduğunu göstermiştir. Maksimum sürgün rejenerasyonu 0.50 mg/L BAP + 0.25 mg/L NAA içeren MS besin ortamında, maksimum kallus oluşumu ise 1 mg/L BAP + 1 mg/L NAA ve 2 mg/L BAP + 1mg/L NAA içeren MS besin ortamında kaydedilmiştir. Rejenere sürgünler 1 mg/L IBA içeren MS besin ortamında köklendirilmiştir.

Anahtar Kelimeler

• BAP, NAA, Aspir, Mikroüretim

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