Süs Kabağında (Cucurbita pepo var. ovifera) Farklı Eksplant Tiplerinde Kallus Çoğalması ve Sürgün Rejenerasyonu

Abstract

The effect of 4 different types of explants (proximal part, distal part, whole cotyledon, and hypocotyl) and 36 different hormone combinations on callus proliferation and shoot regeneration were evaluated in ornamental gourd (Cucurbita pepo var. ovifera). The explants were taken from 8-day-old seedling cultured on Murashige and Skoog (MS) media containing different doses of 2,4dichlorophenoxyacetic acid (2,4-D) (2.0, 2.5, and 3.0 mg l) for callus induction. Generated calli were cultured in combinations of 6-benzylaminopurine (BAP) (0, 0.5, 1.0 and 2.0 mg l) with different doses of α-naphthaleneacetic acid (NAA) (0, 0.1 and 0.5 mg l), indole-3-acetic acid (IAA) (0, 0.1 and 0.5 mg l) and kinetin (0, 1.0 and 2.0 mg l) in MS media. The maximum callus induction ratio (%) in all types of explants was obtained from MS media supplemented with 2.0 mg l2,4-D. BAP (1.0 and 2.0 mg l) combinations with NAA (0.1 and 0.5 mg l) were more successful in hypocotyl and proximal types of explants in terms of callus proliferation (%). The highest mean number of shoots per explant were obtained from 0.5 mg lBAP + 1.0 mg lkinetin in hypocotyl (1.8) and proximal explants (2.6).

Keywords

• Callus, Cucurbita pepo var. ovifera, Organogenesis, Regeneration.

Callus Proliferation and Shoot Regeneration From Different Explant Types in Ornamental Gourd (Cucurbita pepo var.ovifera)

Öz

Süs kabağında (Cucurbita pepo var. ovifera), 4 farklı eksplant tipi (proksimal, distal, tüm kotiledon ve hipokotil) ve 36 farklı hormon kombinasyonunun kallus çoğalması ve sürgün rejenerasyonu üzerine etkisi araştırılmıştır. Kallus uyartımı için 8 günlük fidelerden alınan eksplantlar, Murashige and Skoog (MS) ortamında farklı dozlarda 2,4-D (2.0, 2.5 ve 3.0 mg l) içeren ortamda kültüre alınmıştır. Oluşan kalluslar, MS ortamında BAP’ın (0, 0.5, 1.0, ve 2.0 mg l) kombinasyonları ile birlikte NAA (0, 0.1 ve 0.5 mg l), IAA (0, 0.1, 0.5 mg l) ve kinetinin (0, 1.0 ve 2.0 mg l) farklı dozlarında kültüre alınmıştır. Maximum kallus uyartımı (%) tüm eksplant tiplerinde MS+2.0 mg l2,4-D ortamından elde edilmiştir. Kallus çoğalması (%) yönünden hipokotil ve proksimal eksplant tiplerinde BAP’ın (1.0 ve 2.0 mg l) NAA (0.1 ve 0.5 mg l) ile kombinasyonları daha başarılı bulunmuştur. Eksplant başına en yüksek sürgün sayısı hipokotil (1.8) ve proksimal eksplantlarında (2.6) 0.5 mg lBAP+1.0 mg lkinetin içeren ortamdan elde edilmiştir.

Anahtar Kelimeler

• Cucurbita pepo var. ovifera, Kallus, Organogenesis, Rejenerasyon.

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