Van Gölü Havzası Kavunlarındaki Moleküler Genetik Çeşitliliğin RAPD ve ISSR Belirteçleri İle Belirlenmesi

Abstract

Lake Van Basin of Turkey is located in the secondary gene center for melon. The molecular genetic diversity among thirty-seven melon genotypes collected from Lake Van Basin was determined by RAPD and ISSR makers. Total 121 polymorphic molecular markers obtained from 8 RAPD and 10 ISSR primers were employed to characterize the genetic relationships among the melon genotypes. It was found that within-group genetic similarities ranged between 0.55 and 1.00; related genotypes or genotypes collected from similar regions were partitioned to similar clusters. The genetic diversity parameters among Lake Van Basin melon genotypes were found as H = 0.175 and I = 0.231, and 96.19 % of polymorphism.

Keywords

• Cucumis melo L., Genetic variation, Germplasm, RAPD, ISSR

Molecular Genetic Diversity in Lake Van Basin Melons (Cucumis melo L.) Based on RAPD and ISSR Markers

Öz

Türkiye Van Gölü Havzası kavunun ikincil gen merkezinde yer almaktadır. Van Gölü Havzası’ndan toplanan otuz yedi kavun genotipi arasındaki moleküler genetik çeşitlilik, RAPD ve ISSR belirteçleri yardımıyla belirlenmiştir. Sekiz adet RAPD ve 10 adet ISSR primerinden elde edilen toplam 121 polimorfik moleküler belirteç, kavun genotipleri arasındaki genetik ilişkilerin karakterizasyonunda kullanılmıştır. Grup içi genetik benzerlik katsayısının 0.55 ve 1.00 arasında değiştiği; benzer bölgelerden toplanan genotiplerin veya akraba genotiplerin benzer kümelerde yer aldığı gözlenmiştir. Van Gölü Havzası kavun genotipler arasında genetik çeşitlilik parametreleri olarak H = 0.175, I = 0.231 ve % 96.19 polimorfizm belirlenmiştir.

Anahtar Kelimeler

• Cucumis melo L., Genetik varyasyon, Gen kaynakları, RAPD, ISSR

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