Stimulation Effect of AgNO3 and CoCl2 as Ethylene Inhibitors on in- Vitro Organogenesis of Sunflower (Helianthus annuus L.)

Year 2015, , 113 - 118, 30.05.2015

Fahimeh Mırzaı Ebrahim Ulıaıe Ali Hagh

https://doi.org/10.29133/yyutbd.236424

Cited By: 2

Abstract

To achieve an effective and reliable shoot organogenesis protocol in Helianthus annuus L.,
effect of ethylene inhibitors including AgNO3 and CoCl2 were investigated. Cotyledonary explants of two
cultivars including CMS19 and Progress were cultured in MS medium with 2.0 mg L-1 BAP and 1.0 mg
L-1 IAA supplemented with different concentrations of AgNO3 (0, 1.5 and 3 mg L-1) and CoCl2 (3 and 6
mg L-1). Statistical analysis of data showed a significant difference among cultivars and ethylene
inhibitors concentrations for organogenesis parameters including shoot regeneration and number of shoots
per explant. Addition of ethylene inhibitors to culture medium improved regeneration frequency and
number of shoots per explant. Shoot generation was improved with increasing concentrations of CoCl2
but in the case of AgNO3, increasing the concentration gave adverse results. Treatment of explants with
AgNO3 in low concentration (1.5 mg L-1) improved organogenesis in Helianthus annuus. The highest
shoot regeneration was obtained in media supplemented with 6 mg L-1 CoCl2 (R/E= 63% and S/RE= 3)
and 1.5 mg L-1 AgNO3 (R/E= 73% and S/RE= 2).This study suggests that ethylene inhibitors, particularly
CoCl2, could be used to improve the efficiency of in vitro shoot regeneration and plant transformation
protocols of sunflower.
Key words: Cotyledon, Silver nitrate

Keywords

Cotyledon, Silver nitrate, Cobalt chloride, Helianthus annuus L., Sunflower, Plant regeneration

Ayçiçeği (Helianthus annuus L.)’nin in vitro Organogenezi Üzerine Etilen İnhibitörleri Olarak AgNO3 ve CoCl2’nin Uyarıcı Etkisi

Abstract

Ayçiçeğinde (Helianthus annuus L.) etkili ve güvenilir bir organogenez protokoluna ulaşmak için,
etilen inhibitörleri olarak AgNO3 ve CoCl2‘nin etkisi araştırılmıştır. CMS19 ve Progress çeşitlerinden
elde edilen kotiledon eksplantları 2.0 mg/L BAP ve 1.0 mg/L IAA içeren MS ortamına farklı
konsantrasyonlarda AgNO3 (0, 1.5 ve 3 mg/L) ve CoCl2(3 ve 6 mg/L) ilave edilerek kültüre alınmıştır.
Yapılan istatistik analizine göre; eksplant başına sürgün sayısı ve sürgün rejenerasyonunu içeren
organogenez parametrelerinde etilen inhibitörlerinin konsantrasyonu ve çeşitler arasında istatistiksel
olarak önemli farklılıklar bulunmuştur. Kültür ortamına etilen inhibitörlerinin eklenmesi, eksplant başına
sürgün sayısını ve rejenerasyon frekansını artırmıştır. Sürgün rejenerasyonu CoCl2 konsantrasyonun
artışıyla artmış, ancak AgNO3 konsantrasyonunun artışına bağlı olarak azalmıştır. Düşük konsantrasyonda
(1.5 mg/L) AgNO3 uygulaması ayçiçeğinde (Helianthus annuus) organogenez artırmıştır. En yüksek
sürgün rejenerasyonu ortama 6 mg/l CoCl2 (R/E=% 63 ve S/RE= 3) ve 1.5 mg/L AgNO3 (R/E=% 73 ve
S/RE= 2) eklendiğinde elde edilmiştir. Bu araştırmada etilen inhibitörlerinin, özellikle CoCl2’nın
ayçiçeğinde bitki transformasyon protokolu ve in vitro sürgün rejenerasyon etkinliğinin iyileştirilmesinde
kullanılabileceği önerilmiştir.

Keywords

Kotiledon, Gümüş nitrat, Kobalt klorür, Helianthus annuus L., Ayçiçeği, Bitki rejenerasyonu

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