Research Article

Alleviative Role of Β-Estradiol Against 2,4-Dichlorophenoxyacetic Acid Genotoxicity on Common Bean Genome

Volume: 28 Number: 1 March 30, 2018
TR EN

Alleviative Role of Β-Estradiol Against 2,4-Dichlorophenoxyacetic Acid Genotoxicity on Common Bean Genome

Abstract

2,4-Dichlorophenoxyacetic acid (2,4-D) is one of the several herbicides that widely used to prevent development of Taraxacum officinale and broadleaf weeds on cereal crops culture. However, it is known that 2,4-D can cause genetic damage to plants at low concentrations as well as produce observable physiological effects. There is no report investigating the effect of mammalian hormones in crops against the applied 2,4-D. Therefore, the present study was aimed at investigating levels of DNA damage, changes in DNA methylation and DNA stability in common bean (Phaseolus vulgaris) exposed to 2,4-D and determine whether β-estradiol has any effect. RAPDs (Randomly Amplified Polymorphic DNA) and CRED-RAs (Coupled Restriction Enzyme Digestion-Random Amplification) techniques were used to define the DNA damage levels and changes in the pattern of DNA methylation.  The obtained data demonstrated that 2,4-D led to an increase in RAPDs profile changes (DNA damage), and a reduction in genomic template stability (GTS). The effects caused by 2,4-D were decreased after application with different concentrations of β-estradiol. The results of this study clearly show that β-estradiol could be used function effectively to prevented from the genetic and epigenetic changes caused by 2,4-D herbicide in common bean.

Keywords

References

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Details

Primary Language

English

Subjects

Engineering

Journal Section

Research Article

Publication Date

March 30, 2018

Submission Date

April 11, 2017

Acceptance Date

January 6, 2018

Published in Issue

Year 2018 Volume: 28 Number: 1

APA
Taşpınar, M. S., Sığmaz, B., Aydın, M., Arslan, E., & Ağar, G. (2018). Alleviative Role of Β-Estradiol Against 2,4-Dichlorophenoxyacetic Acid Genotoxicity on Common Bean Genome. Yuzuncu Yıl University Journal of Agricultural Sciences, 28(1), 1-9. https://doi.org/10.29133/yyutbd.305274

Cited By

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Yuzuncu Yil University Journal of Agricultural Sciences by Van Yuzuncu Yil University Faculty of Agriculture is licensed under a Creative Commons Attribution 4.0 International License.