Investigation into Performance of Some Citrus Rootstocks in In vitro Drought Stress Conditions

Abstract

Citrus is a fruit species commercially in the tropical and subtropical regions of the world. Growth and development of plants are affected by environmental factors such as salinity and drought. Global climate change will increase water stress risk in the near future.  One of the purposes of plant biotechnology is the development of plants tolerant to drought. Among environmental stresses, drought stress is one of the factors that negatively affect plant growth and yield. The response of plants to drought stress is quite complex and is the process of many genetic expressions. In this study, Troyer citrange and C-35 citrange from the significant citrus rootstocks were used. After seeds of plant materials were germinated drought stress was applied in in vitro condition. The response of the plants to drought and performance of micropropagation has been determined. It was determined that both rootstocks continued their life and proliferation in increasing PEG doses, however their performance found to be declining.

Keywords

• Abiotic stress,Plant tissue culture,C-35,PEG,Troyer citrange

Bazı Turunçgil Anaçlarının In vitro Kuraklık Stresi Koşullarında Performanslarının Araştırılması

Öz

Turunçgiller dünyanın tropik ve subtropik bölgelerinde ticari olarak yetiştiriciliği yapılan bir meyve türüdür.  Bitkilerin büyüme ve gelişimleri tuzluluk, kuraklık gibi abiyotik faktörlerden etkilenmektedir.  Küresel iklim değişikliğinin yakın gelecekte su stresi riskini artıracağı beklenmektedir.  Bitki biyoteknolojisinin amaçlarından biri kuraklığa tolerant bitkilerin geliştirilmesidir.  Çevresel stresler arasında kuraklık stresi bitki büyüme ve verimini en olumsuz etkileyen faktörlerden biridir.  Bitkilerin kuraklık stresine verdiği cevap oldukça karmaşık ve birçok genin ifadesinin gerçekleştiği bir süreçtir.  Bu çalışmada turunçgil anaçları arasında yer alan Troyer sitranjı ve C-35 sitranjı kullanılmıştır.  Bitkisel materyallere ait tohumlar çimlendirildikten sonra in vitro koşullarda kuraklık stresi uygulanmıştır.  Bitkilerin in vitro’da kuraklık stresi altında çoğaltım performansları ve verdikleri tepkiler belirlenmiştir.  Her iki anacında artan PEG dozlarında yaşamları ve çoğalmalarına devam ettirdikleri ancak performanslarının gerilediği tespit edilmiştir.  

Anahtar Kelimeler

• PEG,Abiyotik stres,Bitki doku kültürü,C-35,Troyer sitranjı

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