Effect of Different Hormone Applications on Antioxidant Enzyme Activities of Strawberry Leaves

Abstract

This study was carried out to determine the effect of the application of methyl jasmonate (MeJa) and gibberellic acid (GA₃) on the antioxidant enzyme activity in 5 different strawberry cultivars (Albion, Aromas, Honeoye, Seascape, Sweet Ann). The application of 50 ppm and 100ppm GA₃ and 0.25, 0.50, and 1.00 mM doses MeJA was applied as foliar spray. Catalase (CAT), superoxide dismutase (SOD) and malondialdehyde MDA levels were determined in the collected leaf. Samples sweet Ann cultivar had the highest CAT and SOD activity while Honeoye cultivar had the lowest values. CAT activity was decreased in Seascape and Aromas cultivars, and it was observed that CAT activity variability according to application and dose in other cultivars. The amount of SOD has increased with each application in the Albion and Seascape cultivars. In contrast to CAT and SOD activity, MDA concentration was determined the highest in Honeoye and the lowest in Sweet Ann. The present study, it was determined that the hormone (GA₃, MeJa) applications affected the enzyme and MDA contents depending on the cultivars and the doses of the hormones.

Keywords

• Antioxidant enzymes,Plant hormones,Strawberry,MDA

Çilek Yapraklarının Antioksidan Enzim Aktiviteleri Üzerine Farklı Hormon Uygulamalarının Etkisi

Öz

Bu çalışma, 5 farklı çilek çeşidinde (Albion, Aromas, Honeoye, Seascape, Sweet Ann) dışardan metil jasmonat (MeJA) ve gibberalik asit (GA₃) uygulamasının antioksidan enzim aktiviteleri üzerine etkisini belirlemek amacıyla yapılmıştır. 50ppm ve 100ppm dozlarında GA₃ ve 0.25, 0.50 ve 1.00 mM dozlarında MeJA uygulaması yapraktan uygulanmıştır. Alınan yaprak örneklerinde katalaz (KAT), süperoksidaz dismutaz (SOD) ve malondialdehit (MDA) miktarları okunmuştur. Sweet Ann çeşidi en yüksek KAT ve SOD aktivitesine sahipken, Honeoye çeşidi en düşük değerlere sahip olmuştur. CAT aktivitesi Seascape ve Aromas çeşitlerinde azalma göstermiş diğer çeşitlerde uygulama ve doza bağlı olarak değişkenlik görülmüştür. SOD miktarı Albion ve Seascape çeşitlerinde her uygulama ile artış kaydetmiştir. KAT ve SOD aktivitesinin aksine, MDA konsantrasyonu en fazla Honeoye çeşidinde, en düşük Sweet Ann çeşidinde belirlenmiştir. Yapılan araştırmada, GA₃ ve MeJa uygulamaları çilek çeşitlerine ve uygulanma dozlarına bağlı olarak yaprakların enzim ve MDA içeriklerini etkilediği saptanmıştır.

Anahtar Kelimeler

• Çilek,Antioksidan enzimler,Bitkisel hormonlar,MDA

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