Biological Control of Phytophthora Blight of Pepper with Antagonistic Bacteria

Abstract

The aim of this study was to investigate the possibilities of biological control of Phytophthora blight in pepper by using antagonistic bacteria. For biological control studies, antagonist bacteria were isolated from pepper roots. Antimicrobial effects of bacterial antagonists against disease agent was tested by dual culture, radicle assay, potting and field experiments. A total of 95 isolates of antagonists were isolated during the study, of which 21 had mycelial inhibition rates of 20-82% in the dual culture tests. Bacterial antagonists were found to be effective for suppression of disease development up to 93% in radicle assay. Some of the antagonists tested in the pot experiments inhibited the disease, however others were found to increase disease severity. Promising results were obtained in field trials.

Keywords

• Biocontrol,Radicle assay,Dual culture,qPCR,Phytophthora capsici

Biberde Phytophthora Yanıklığına Karşı Antagonist Bakterilerle Biyolojik Mücadele

Öz

Bu çalışma ile biberde Phytophthora yanıklığı hastalığına karşı antagonist bakteriler ile biyolojik mücadele olanakları araştırılmıştır. Biyolojik mücadele çalışmalarında kullanılmak üzere biber köklerinden antagonist bakteri adayları izole edilmiştir. Daha sonra antagonistlerin hastalık etmenine karşı olan antimikrobiyal etkileri ikili kültür, kökçük testi, saksı ve tarla denemeleri ile değerlendirilmiştir. Çalışmalar sırasında toplam 95 adet antagonist bakteri izolatı elde edilirken, bunlardan 21 tanesi ikili kültür testlerinde hastalık etmeninin misel gelişimini %20-82 oranında engellemiştir. Kullanılan antagonistlerin yapılan kökçük testlerinde hastalık çıkışını %93’e varan bir oranda engellemek suretiyle etkili olduğu saptanmıştır. Yapılan saksı denemelerinde test edilen antagonistlerden bazıları hastalık etmenini engellerken bazılarının da hastalık şiddetini arttırdığı bulunmuştur. Biyolojik mücadelenin değerlendirildiği tarla denemelerinde ise ümitvar sonuçlar elde edilmiştir.

Anahtar Kelimeler

• Biyokontrol,Kökçük testi,İkili kültür,qPCR,Phytophthora capsici

References

1. Akgül, S. D., & Mirik, M. (2008). Biocontrol Of Phytophthora capsici On Pepper Plants By Bacillus megaterium Strains. J. Plant Pathol., 90(1), 29–34.
2. Berg, G., Roskot, N., Steidle, A., Eberl, L., Zock, A., & Smalla, K. (2002). Plant-Dependent Genotypic and Phenotypic Diversity of Antagonistic Rhizobacteria Isolated from Different Verticillium Host Plants. Applied and Environmental Microbiology, 68(7), 3328–3338.
3. Cenis, J. L. (1992). Rapid extraction of fungal DNA for PCR amplification. Nucl Acids Res., 20(9), 2380.
4. Chang, S. H., Kwack, M. S., Kim, Y. S., Lee, J. Y., & Kim, K. D. (2001). A Rapid Radicle Assay for Prescreening Antagonistic Bacteria Against Phytophthora capsici on Pepper. Mycobiology, 29(4), 218–223.
5. Dinu, A., Kumar, A., Aravind, R., & Eapen, S. J. (2007). An improved method for selection of antagonistic bacteria against Phytophthora capsici Leonian infections in black pepper (Piper nigrum L.). Journal of Spices and Aromatic Crops, 16(1), 1–7.
6. EPPO. (2015). PQR - EPPO database on quarantine pests. http://www.eppo.int Erişim tarihi: 10.08.2015.
7. Erwin, D. C., & Ribeiro, O. K. (1996). Phytophthora Diseases Worldwide. APS Press.
8. Fierer, N., Jackson, J. A., Vilgalys, R., & Jackson, R. B. (2005). Assessment of Soil Microbial Community Structure by Use of Taxon-Specific Quantitative PCR Assays. Applied and Environmental Microbiology, 71(7), 4117–4120.

9. Hausbeck, M. K., & Lamour, K. H. (2004). Phytophthora capsici on Vegetable Crops: Research Progress and Management Challenges. Plant Dis., 88(12), 292–1303.
10. Jiang, Z. Q., Guo, Y. H., Li, S. M., Qi, H. Y., & Guo, J. H. (2006). Evaluation of biocontrol efficiency of different Bacillus preparations and field application methods against Phytophthora blight of bell pepper. Biological Control, 36, 216–223.
11. Kim, H. S., Sang, M. K., Jung, H. W., Jeun, Y. C., Myung, I. S., & Kim, K. D. (2012). Identification and characterization of Chryseobacterium wanjuense strain KJ9C8 as a biocontrol agent of Phytophthora blight of pepper. Crop Protection, 32, 129–137.
12. Kim, S. G., Jang, Y. L., Kim, H. Y., Koh, Y. J., & Kim, Y. H. (2010). Comparison of Microbial Fungicides in Antagonistic Activities Related to the Biological Control of Phytophthora Blight in Chili Pepper Caused by Phytophthora capsici. Plant Pathol. J., 26(4), 340–345.
13. Klement, Z., Rudolph, K., & Sands, D. C. (1990). Methods in phytobacteriology. Budapest, Hungary: Akademiai Kiadó.
14. Kurze, S., Bahl, H., Dahl, R., & Berg, G. (2001). Biological Control of Fungal Strawberry Diseases by Serratia plymuthica HRO-C48. Plant Dis., 85(5), 529–534.
15. Lan, C. Z., Liu, P. Q., Li, B. J., Chen, Q. H., & Weng, Q. Y. (2012). Development of a specific PCR assay for the rapid and sensitive detection of Phytophthora capsici. Australasian Plant Pathology, 42(3), 379–384.
16. Mei, X., Zhao, Q., Tan, S., Xu, Y., Shen, B., & Shen, Q. (2010). Screening, identification, and biocontrol effect of antagonistic bacteria against Phytophthora capsici. The Journal of Applied Ecology, 21(10), 2652–2658.
17. Özyılmaz, Ü. (2007). Biological Control of Strawberry Root Diseases by Rhizobacteria in Aydin Province. PhD thesis, Adnan Menderes Univ., Turkey.
18. Özyilmaz, Ü., & Benlioglu, K. (2013). Enhanced Biological Control of Phytophthora Blight of Pepper by Biosurfactant-Producing Pseudomonas. Plant Pathol. J., 29(4), 418–426.
19. Pal, K. K., & Mc Spadden Gardener, B. (2006). Biological Control of Plant Pathogens, The Plant Health Instructor. http://dx.doi.org/10.1094/phi-a-2006-1117-02 Erişim tarihi 24.07.2019.
20. Pang, Z., Shao, J., Chen, L., Lu, X., Hu, J., Qin, Z., & Liu, X. (2013). Resistance to the Novel Fungicide Pyrimorph in Phytophthora capsici: Risk Assessment and Detection of Point Mutations in CesA3 That Confer Resistance. PLoS ONE, 8(2), 1–12.
21. Parra, G., & Ristaino, J. B. (2001). Resitance to mefenoxam and metalaxil among field isolates of Phytophthora capsici causing phytophthora blight of bell peper. Plant Disease, 85, 1069–1075.
22. Pavón, C. F., Babadoost, M., & Lambert, K. N. (2008). Quantification of Phytophthora capsici Oospores in Soil by Sieving-Centrifugation and Real-Time Polymerase Chain Reaction. Plant Disease, 92(1), 143–149.
23. Qi, R., Wang, T., Zhao, W., Li, P., Ding, J., & Gao, Z. (2012). Activity of Ten Fungicides against Phytophthora capsici Isolates Resistant to Metalaxyl. Journal of Phytopathology, 160(11–12), 717–722.
24. Robles-Yerena, L., Rodríguez-Villarreal, R. A., Ortega-Amaro, M. A., Fraire-Velázquez, S., Simpson, J., Rodríguez-Guerra, R., & Jiménez-Bremont, J. F. (2010). Characterization of a new fungal antagonist of Phytophthora capsici. Scientia Horticulturae, 125, 248–255.
25. Sagir, A., & Yildiz, M. (1987). Studies on the reaction of the common vegetable varieties to some Phytopthora spp. isolates. Bitki Koruma Bülteni, 27(3), 179–200.
26. Sang, M. K., Chun, S. C., & Kim, K. D. (2008). Biological control of Phytophthora blight of pepper by antagonistic rhizobacteria selected from a sequential screening procedure. Biological Control, 46, 424–433.
27. Sanzani, S. M., Li Destri Nicosia, M. G., Faedda, R., Cacciola, S. O., & Schena, L. (2014). Use of Quantitative PCR Detection Methods to Study Biocontrol Agents and Phytopathogenic Fungi and Oomycetes in Environmental Samples. Journal of Phytopathology, 162, 1–13.
28. Schaad, N. W., Jones, J. B., & Chun, W. (2001). Laboratory Guide for Identification of Plant Pathogenic Bacteria. 3rd ed. APS Press, St. Paul Minnesota.
29. Sopheareth, M., Chan, S., Naing, K. W., Lee, Y. S., Hyun, H. N., Kim, Y. C., & Kim, K. Y. (2013). Biocontrol of Late Blight (Phytophthora capsici) Disease and Growth Promotion of Pepper by Burkholderia cepacia MPC-7. The Plant Pathology Journal, 29(1), 67–76.
30. Zhang, S., Klassen, W., Mo, X., Ji, P., & Gevens, A. J. (2011). Evaluation of Acibenzolar-S-methyl and silicic acid for control of Phytophthora blight caused by Phytophthora capsici in squash. Proc. Fla. State Hort. Soc., 124, 154–161.