Determination of Some Important Phosphorus Solubilising Fungi Secreting Lactic Acid and Fumaric Acid in Soil

Abstract

This study was carried out with the aim of isolation, microscopic identification and determination of organic acid synthesis of phosphorus (P) solubilising fungi from rhizosphere soil part of different plants grown in different agricultural areas in Amasya province. Eight different phosphorus- solubilising fungi were isolated from the rhizosphere soil using NBRIP (National Botanical Research Institute's Phosphate) solid medium. Fungi were identified at the genus level as Botrytis spp., Alternaria spp. Rhizopus spp., Penicillium spp., Fusarium spp., Trichoderma spp., Aspergillus spp. and Cladosporium spp. according to spore characteristics. Penicillium spp isolate was determined quantitatively the highest rate of P soluble isolates with 52.98 mg/ml and Cladosporium spp. isolate the lowest amount of P dissolving with 29.8 mg/ml. At the same time, the total amount of Lactic and Fumaric organic acid secreted by fungus isolates is directly proportional to P and inversely proportional to pH. It was determined Penicillium spp isolate with 12.475 mg/ml, which synthesizes the highest amount of organic acid, while Cladosporium spp. with the lowest rate of 10.268 mg/ml. As a result, it was determined that the identified fungi were associated with the total amount of organic acid they synthesized with the P solubilising.

Keywords

• Phosphorus solubilising
• Fungi
• Fumaric acid
• Lactic acid
• NBRIP
• Organic acid

Project Number

FMP BAP 18-0373

Toprakta Laktik Asit ve Fumarik Asit Salgılayan Önemli Bazı Fosfat Çözücü Fungusların Belirlenmesi

Abstract

Bu çalışma, Amasya ilindeki farklı tarım alanlarında yetişen farklı bitkilerin rizosfer kısmındaki topraktan fosfor (P) çözen fungusların izolasyonu, mikroskobik tanılanması ve organik asit sentezlerinin belirlenmesi amacı ile yapılmıştır. Rizosfer toprağından NBRIP (National Botanical Research Institute’s Phosphate) katı besiyeri kullanılarak 8 farklı fosfor çözen fungus izole edilmiştir. Funguslar spor karakteristik özelliklerinegöre cins düzeyinde Botrytis spp., Alternaria spp. Rhizopus spp., Penicillium spp., Fusarium spp., Trichoderma spp., Aspergillus spp. ve Cladosporium spp. olarak belirlenmiştir. İzolatlar arasında nicel olarak en yüksek oranda P çözen izolatın 52.98 mg/ml ile Penicillium spp izolatı belirlenirken en düşük miktarda P çözen 29.8 mg/ml ile Cladosporium spp. izolatı belirlenmiştir. Aynı zamanda fungus izolatlarının salgıladıkları toplam Laktik ve Fumarik organik asit miktarlarının çözünen P ile doğru orantılı ve pH ile ters orantılı olduğu belirlenmiştir. İzolatlar arasında en yüksek oranda organik asit sentezleyen 12.475 mg/ml ile Penicillium spp. izolatı olurken, en düşük oranda 10.268 mg/ml ile Cladosporium spp. izolatı belirlenmiştir. Sonuç olarak bu çalışmada fungusların çözdüğü P ile sentezledikleri toplam organik asit miktarı ile ilişkili olduğu belirlenmiştir.

Keywords

• Organik asit
• Fosfor Çözücü Fungus
• NBRIP
• Laktik asit
• Fumarik Asit

Supporting Institution

Amasya Üniversitesi

Project Number

FMP BAP 18-0373

Thanks

Bu çalışma Amasya Üniversitesi Bilimsel Araştırma Projeleri birimi tarafından FMP BAP 18-0373 no’lu proje kapsamında desteklenmiştir.

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