Determination of Genetic Relations among Tomato Accessions in Sulaymaniyah Region through ISSRs Markers Genetic Relations in Tomato Accessions

Abstract

The goal of this study was to examine the genetic difference among thirty two tomato accessions (Solanum lycopersicum L.) grown in Sulaymaniyah using ISSR molecular markers technique. The results of ISSR markers revealed 65 amplified fragments, 50 of them were polymorphic from using 15 primers. Fifteen ISSR markers used to detect DNA polymorphism gave polymorphism percentage for each primer range between 50–100% with an average polymorphism percentage reaching 75.61%. ISSR6 and Sola 11 gave the highest polymorphism percentage was 100%, while 3F, Sola 5 and Sola 12 did not give any amplification. The mean of PIC (Polymorphic Information Content) value was 0.50 for ISSR markers. The similarity matrix was obtained by using Jaccard’s coefficients, based on polymorphic bands and dendrogram constituted through UPGMA cluster analysis. The dendrogram revealed 4 main groups. Genetic similarity (GS) ranged from 0.261 to 0.941 within studied accessions. The highest similarity was 0.941 for the genotype pairs G7 and G16. However, the lowest similarity index was 0.261 among G15-G24 and G15-G26. Tomato accessions were determined by two populations according to STRUCTURE analysis. The ISSR marker system ensured useful information in describing genetic diversity among tomato accessions collected from different geographical around Sulaymaniyah province.

Keywords

• Genetic variation
• Sulaymaniyah
• ISSR
• Solanum lycopersicum

Süleymaniye Bölgesindeki Domates Genotipleri Arasındaki Genetik İlişkilerin ISSR Belirteçleri ile Belirlenmesi

Abstract

Çalışmada Süleymaniye bölgesinde yetiştirilen 32 domates (Solanum lycopersicon L.) genotipi arasındaki genetik çeşitliliğin ISSR belirteç tekniği kullanarak belirlemek amaçlanmıştır. ISSR primerlerinin sonuçları, 15 primerden 65 amplifikasyon oluştuğunu ve bu amplifikasyonların 50’sinin polimorfik olduğunu ortaya çıkarmıştır. Polimorfizmi belirlemek için kullanılan 15 ISSR primeri % 50-100 arasında polimorfizm oranına sahip olmuş ve ortalama polimorfizm oranının % 75.61 olduğu belirlenmiştir. ISSR primerlerinin PIC değeri ortalaması 0.50 olarak tespit edilmiştir. Benzerlik matriksi polimorfik bant varlığın bağlı olarak Jaccard benzerlik katsayısı ile hesaplanmış ve UPGMA yöntemine göre kümeleme analizi ile dendogram elde edilmiştir. Dendogramda 4 temel grup oluştuğu belirlenmiştir. Genotipler arasındaki genetik benzerlik (GS) katsayıları 0.261 ile 0.941 arasında değişiklik göstermiştir. En yüksek benzerlik 0.941benzerlik katsayısı ile G7 ve G16 genotipleri arasında olurken, en düşük benzerlik 0.261 ile G15-G24 ve G15-G26 arasında bulunmuştur. STRUCTURE analizine göre ise genotiplerin iki populasyona ayrıldıkları saptanmıştır. ISSR yönteminin Süleymaniye’nin farklı bölgelerinden toplanmış domates genotipleri arasındaki genetik çeşitliliği tanımlamada etkili olduğu belirlenmiştir.

Keywords

• Genetik çeşitlilik
• ISSR
• Solanum lycopersicum
• Süleymaniye

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