In Vitro Propagation of Some Mahaleb Genotypes as Candidate Rootstock for Sweet Cherries

Abstract

In this study, where carried out in 3 mahaleb genotypes and SL 64 (St. Lucie 64) rootstocks which can be rootstock candidate for cherry were investigated in order to determine the multiplication performance of in vitro conditions, shoot tips were used as explant of with mahaleb genotypes and SL 64 rootstocks. Surface sterilization was applied to the explants in the initial stage 0.5 mg l-1 BAP (6-Benzylaminopurine) +0.1 mg l-1 GA3 (Gibberellic acid) + 0.1 mg l-1IBA (Indole-3-butyric acid) was cultured in MS (Murashige and Skoog) medium. The study was carried out to determine the effect of different BAP (0, 0.5 and 1 mg l-1) doses during the shoot multiplication stage, the number of shoots was 1.97-2.55 in mahaleb genotypes. While the number of shoots increased with increasing BAP dose, the maximum number of shoots was obtained from 1 mg l-1 BAP dose. In the rooting stage, 0, 0.5, 1 and 2 IBA was added to ½ MS medium. The effect of different doses of IBA (0, 0.5, 1, 2 mg l-1) in rooting medium was investigated. While the best results in terms of callus ratio, rooting rate and root length were obtained from 2 mg l-1 IBA dose, the best results in terms of rooted plant length and number of leaves were obtained from 1 mg l-1 IBA dose.

Keywords

• Micropropagation
• MS
• Prunus avium
• Rooting
• Rootstock

Kiraz İçin Anaç Adayı Bazı Mahlep Genotiplerinin İn vitro Çoğaltımı

Abstract

Kiraz için anaç adayı olabilecek 3 adet mahlep genotipi ve SL 64 (St. Lucie 64) anacınınım in vitro şartlarında çoğaltılabilme olanakları araştırıldığı bu çalışmada eksplant olarak mahlep genotipleri ve SL 64 anacının sürgün uçları kullanılmıştır. Eksplantlar yüzey sterilizasyonu yapılarak başlangıç aşamasında 0.5 mg/l BAP (6-Benzilaminopurin) + 0.1 mg l-1 GA3 (Gibberellic acid) + 0.1 mg/l IBA (İndole-3-butyric acid) içeren MS (Murashige ve Skoog) besi ortamında kültüre alınmıştır. Çoğaltma ortamında farklı BAP (0, 0.5 ve 1 mg/l) dozlarının etkisini belirlemek için yapılan çalışmada sürgün sayısı 1.97-2.55 adet arasında değişmiştir. BAP dozunun artması ile sürgün sayısının arttığı belirlenirken, en fazla sürgün sayısı 1 mg/l BAP dozundan elde edilmiştir. Köklendirme aşamasında ise farklı IBA dozlarının (0, 0.5, 1 ve 2 mg/l) etkisi araştırılmıştır. Kallus oranı, köklenme oranı ve kök uzunluğu bakımından en iyi sonuçlar 2 mg/l IBA dozundan elde edilirken, köklü bitkinin uzunluğu ve yaprak sayısı bakımından ise en iyi sonuçlar 1 mg/l IBA dozundan elde edilmiştir.

Keywords

• Prunus avium
• MS
• Mikroçoğaltım
• MS
• Köklenme
• Anaç

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