The effect of humic acid on rapeseed (Brassica napus L.) plant growth, heavy metal uptake, phytoremediation parameters (BCF, TF and TI), and antioxidant activity in heavy metal polluted soil

Year 2022, , 237 - 248, 30.06.2022

Sibel Boysan Canal , Mehmet Ali Bozkurt , Hilal Yılmaz

https://doi.org/10.29133/yyutbd.997850

Cited By: 2

Abstract

The aim of this study was to investigate the effects of humic acid (HA) applications on rapeseed (Brassica napus L.) growth, heavy metal uptake, bioconcentration factor (BCF), translocation factor (TF), tolerance index (TI), catalase (CAT), ascorbate peroxidase (APX) enzyme activities and hydrogen peroxide (H2O2) content in polluted soil with lead (Pb), chromium (Cr), cadmium (Cd), and zinc (Zn). Three doses of HA (Control, HA1:500 mg kg-1, HA2:1000 mg kg-1, HA3:2000 mg kg-1) were applied in pots. HA1, HA2, and HA3 applications increased plant growth parameters compared to polluted soil. Compared to the control, HA applications in polluted soil increased the Pb, Cr, Cd, and Zn concentrations in the plant. However, HA applications in polluted soil significantly decreased the heavy metal content in roots and shoots of the plant compared to polluted soil. BCF in both roots and shoots of the plants were greater than 1 for Pb, Cr, Cd, and Zn. However, specifically HA2 application decreased the shoot and root BCF values in polluted soil. TF was smaller than 1 in Pb, Cr, Cd, and Zn in polluted soil. On the other hand, HA applications for Cd increased TF values. Shoot TI decreased 17.37 %, and root TI decreased 9.09% in polluted soil. CAT and APX enzyme activities and H2O2 increased significantly in polluted soil. However, HA applications decreased CAT and APX enzyme activities and H2O2 content in rapeseed. It is concluded that HA application in Pb, Cr, Cd, and Zn polluted soil has a remedial effect on the development of rapeseed by reducing heavy metal content and oxidative stress.

Keywords

Antioxidative activity, Heavy Metal, Humic Acid, Plant Growth, Rapeseed (Brassica napus L)

Project Number

FBA-2018-6511

References

• Referans1 Alım, Z. (2020). Hümik asit uygulamalarının ağır metal stresi altında yetiştirilen terede bitki gelişimi ile bazı fizyolojik ve biyokimyasal özellikler üzerine etkileri, Atatürk Üniversitesi, Fen Bilimleri Enstitüsü, Bahçe Bitkileri Bölümü, Yüksek Lisans Tezi, Erzurum.
• Referans2 Alpay, Ş. (2013). Hümik asit esaslı adsorban maddelerle ağır metal giderimi.Gazi Üniversitesi Fen Bilimleri Enstitüsü,Çevre Mühendisliği Bölümü, Yüksek Lisans Tezi,Ankara.
• Referans3 Alpaslan M, Güneş A & Inal A (1998). Deneme Tekniği Ankara Üniversitesi Ziraat Fakültesi Yayınları: 1501, Ders Kitabı:423, Ankara.
• Referans4 Ali S, Bharwana S A, Rizwan M, Farid M, Kanwal S,Ali Q, Ibrahim M, Gill R A &Khan M D (2015). Fulvic acid mediates chromium (Cr) tolerance in wheat (Triticum aestivum L.) through lowering of the Cr uptake and improved antioxidant defense system. Environ Sci Pollut Res 22: 10601–10609.
• Referans5 Ali S, Rizwan M, Waqas A, Hussain M B, Hussain A, Liu S, Alqarawi A A, Hashem A& Abd_Allah E F (2018). Fulvic acid prevents chromium-induced morphological, photosynthetic, and oxidative alterations in wheat irrigated with tannery wastewater. J. Plant Growth Regul. 37: 1357–1367.
• Referans6 Bouyoucus, G., D. (1951). A. recalibration of the hydrometer method for making machanical analysis of soil. Agronomy J. 43:434-438.
• Referans7 Cozzolino, V.; De, M. A.; Nebbioso, A.; Di, M. V.; Salluzzo, A.& Piccolo, A. (2016). Plant tolerance to mercury in a contaminated soil is enhanced by the combined effects of humic matter addition and inoculation with arbuscular mycorrhizal fungi. Environ. Sci. Pollut. Res. 23, 11312−11322.
• Referans8 Çakmak I, Strbac D. & Marschner, H. (1993). Activities of hydrogen peroxide-scavenging enzymes in germinated wheat seedlings. J. Exp. Bot. 44:127-132.
• Referans9 Çifçi, A., (2020). Çoklu metal (Cd, Pb, Zn) ile kirlenmiş toprakların fitoremediasyon ve immobilizasyonla arıtımında zeolit, nanozeolit ve edds uygulamasının etkisi ve sağlık risk analizi. Mersin Üniversitesi Fen Bilimleri Enstitüsü Çevre Mühendisliği Bölümü, Doktora Tezi, Mersin.
• Referans10 Çiftçi, A. (2016). Çoklu metal (kadmiyum, kurşun ve çinko) ile kirlenmiş bir toprağın arıtımında yabani hint yağı (Ricinus communis) ve aspir (Carthamus tinctorius) bitkilerinin fitoremediasyon kapasitesinin araştırılması. Mersin Üniversitesi Fen Bilimleri Enstitüsü, Yüksek Lisans Tezi, Mersin.
• Referans11 Çimrin, K.M., Karaca, S. & Bozkurt, M.A. (2001). The effect of NPK and humic acid applications on growth and nutrition of corn plant (Zea mays L.). Ankara University. J. Agric. Sci. 7: 95-100.
• Referans12 Düzgüneş, A., Kesici, O.T., Kavuncu, O., & Gürbüz, F., (1987). Araştırma ve Deneme Metodları (İstatistik Metodları-II). Ankara Üniversitesi Ziraat Fakültesi Yayınları, 1021:381.
• Referans13 Dobbss, L.B., dos Santos, T.C., Pittarello, M., de Souza, S. B., Ramos, A. C., & Busato, J. G. (2018). Alleviation of iron toxicity in Schinus terebinthifolius Raddi (Anacardiaceae) by humic substances. Environmental Science and Pollution Research 25, 9416–9425.
• Referans14 Esringü, A., Turan, M., Güne, M., & Karaman, R. M. (2014). Roles of bacillus megaterium in remediation of boron, lead, and cadmium from contaminated soil. Commun Soil Sci Plan 45, 1–19.
• Referans15 Evangelou, M. W., Daghan, H., & Schaeffer, A. (2004). The influence of humic acids on the phytoextraction of cadmium from soil. Chemosphere 57(3), 207-213.
• Referans16 Gülser, F., & Ayaş, H., (2016). Kükürt ve humik asit uygulamalarının ıspanak (Spinacea oleracea var. Spinoza) bitkisinin mikro besin elementi içeriklerine etkisi. Toprak Bilimi ve Bitki Besleme Dergisi 4(1), 27-31.
• Referans17 García, A.C., Santos, L. A., Souza, L. G. A., Tavares, O. C. H., Zonta, E., Gomes, E. T. M., García-Mina, J. M., & Berbara, R. L. L. (2016). Vermicompost humic acids modulate the accumulation and metabolism of ROS in rice plants. J Plant Physiol 192, 56–63. https://doi.org/10.1016/j.jplph.
• Referans18 Hamilton, C. E., Gundel, P. E., Helander, M., & Saikkonen, K. (2012). Endophytic mediation of reactive oxygen species and antioxidant activity in plants: a review. Fungal Divers 54, 1–10. doi:10.1007/s13225-012- 0158-9.
• Referans19 Hızalan, E., & Ünal, E. (1966). Topraklarda Önemli Analizler. Ank.Ünv. Zir. Fak.,Yayın no.278.
• Referans20 İbrikçi, H., Gülüt, Y. K., & Güzel, N. (1994). Gübrelemede Bitki Analiz Tekniği. Çukurova Üniversitesi Ziraat Fakültesi Yayınları. No: 95. Adana 85.
• Referans21 Jackson, M.L. (1958). Soil Chemical Analysısprentice hall, ınc. New Jersey,USA.
• Referans22 Kacar, B. (1994). Bitki ve toprağın kimyasal analizleri: III Toprak Analizleri. A.Ü.Z.Eğitim Araştırma ve Geliştirme vakfı yayınları No:3 Ankara 705.
• Referans23 Kwiatkowska-Malina, J. (2018). Functions of organic matter in polluted soils: The effect of organic amendments on phytoavailability of heavy metals. Applied Soil Ecology 123, 542-545. https://doi.org/10.1016/j.apsoil. 2017.06.021.
• Referans24 Khan, K. D., & Frankland, B. (1983). Chemical Forms of Cd and Pb in some contamineted soils. Enviromental Pollution (B) 6, 15-31.
• Referans25 Kürşat, Z., (1999). “Bazı Crambe L. türleri üzerinde morfolojik, anatomik ve palinolojik çalışmalar”, Osmangazi Üniversitesi, Fen Bilimleri Enstitüsü, Biyoloji ABD, Yüksek Lisans Tezi, Eskişehir.
• Referans26 Lagier, T., Feuillade, G., & Matejka, G. (2000). Interactions between copper and organic macro molecules:Determination of conditional complexation constants. Agronomie, 20537–546.
• Referans27 Lindsay, W. L., & Norvell, W.A. (1978). Development of a DTPA soil test for zinc, iron, manganese and copper. Soil.Sci.Soc. Am.J 42, 421-428.
• Referans28 Munir, M. A. M., Liu, G., Yousaf, B., Mian, M. M., Ali, M. U., Ahmed, R. Ayesha, I. C., & Mu, Naushad. (2020). Contrasting effects of biochar and hydrothermally treated coal gangue on leachability, bioavailability, speciation and accumulation of heavy metals by rapeseed in copper mine tailings. Ecotoxicol. Environ., 191.
• Referans29 Nakano, Y, Asada, K. (1981). Hydrogen peroxide in spinach chloroplasts. Plant Cell Physiol. 22, 860-867.
• Referans30 Nardi, S., Carletti, P., Pizzeghello, D., & Muscolo, A. (2009). Biological activities of humic substances. In: Biophysico-chemical processes involving natural nonliving organic matter in environmental systems. (Eds.): Senesi, N., B. Xing and P.M. Huang. Wiley, Hoboken, pp. 305-339.
• Referans31 Okcu, M., Tozlu, E., Kumlay, A. M., & Pehluvan, M. (2009). Ağır Metallerin Bitkiler Üzerine Etkileri. Alınteri Dergisi 17, 14- 26.
• Referans32 Özkay, F., Kıran, S., Kuşvuran, Ş., & Ellialtıoğlu, Ş. Ş. (2016). The effects of humic acid applications on heavy metal stress in lettuce. Turkish Journal of Agriculture-Food Science And Technology 4(6), 431-437.
• Referans33 Özbek, K. (2015). Hiperakümülasyon ve Türkiye florasındaki hiperakümülatör türler. Toprak Bilimi ve Bitki Besleme Dergisi 3(1), 37-43.
• Referans34 Pandey, V. C (2013). Suitability of Ricinus communis L. cultivation for phytoremediation of fly ash disposal sites. Ecological Engineering 57, 336-341.
• Referans35 Rastghalam, Z. S., Hoodaji, M., & Javanmard, H. (2011). The ınfiuence of humic acid and nano-superabsorbent application on the grovvth of brassica napus l. in lead-contaminated soil, International Conference on Environmental and Agriculture Engineering, 29th to 31st July 2011, Chengdu, China.
• Referans36 Rascio, N., & Navari-Izzo, F. (2011). Heavy Metal Hyperaccumulating Plants: How and Why do They do it? And What Makes them so Interesting? Plant Science 180, 169-181.
• Referans37 Singh, J., & Kalamdhad, A. S. (2011). Effects of Heavy Metals on Soil, Plants, Human Health and Aquatic Life. International Journal of Research in Chemistry and Environment 1, 15-21.
• Referans38 Turan, M., & Esringu, A. (2007). Phytoremediation based on canola (Brassica napus L.) and Indian mustard (Brassica juncea L.) planted on spiked soil by aliquot amount of Cd, Cu, Pb, and Zn. Plant Soil Environ. 53, 7–15.
• Referans39 Velikova, P., Yordanov, I., & Edreva, A., (2000). Oxidative stress and some antioxidant systems in acid rain-treated bean plants. Protective role of exogenous polyamines. Plant Science.15, 59–66.
• Referans40 Walkley, A., (1947). A. critical examination of a rapid metod for determining organic carbon in soils: Effect of variation in digestion conditions and inorganic soil constituent Soil.Sci.63, 251-263.
• Referans41 Xu, Q., Duan, D. C., Cai, Q. Y., & Shi, J. Y. (2018). Influence of humic acid on Pb uptake and accumulation in tea plants. J Agric Food Chem 66, 12327–12334.
• Referans42 Zayed, A., Lytle, C. M., Qian, J. H., & Terry, N. (1998). Chromium accumulation, translocation and chemical speciation in vegetable crops. Planta, 206(2), 293-299.