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Bazı Patlıcan Genotiplerinin Tuz Stresine Antioksidatif Tepkileri

Year 2013, Volume: 23 Issue: 3, 215 - 221, 01.09.2013

Abstract

The response of the antioxidant enzymes to salt stress was studied in the eggplant genotypes. In this study, two salt-tolerant Burdur Bucak (BB) and Mardin Kızıltepe (MK); two salt-sensitive Giresun (Gi) and Artvin Hopa (AH), four of them belong to Solanum melongena L. species are landraces grown different parts of Turkey, and a salt tolerant wild species S. sisymbriifolium (SS) were used. The antioxidant activities of superoxide dismutase (SOD; EC 1.15.1.1), catalase (CAT; EC 1.11.1.6), ascorbate peroxidase (APX; EC 1.1.1.11) and glutathione reductase (GR; EC 1.6.4.2) enzymes were investigated in these salt-tolerant and sensitive genotypes grown in hydroponics culture. At the end of the study, it was determined that all of the antioxidant enzymes were very effective on the salt tolerance, and salt-tolerant eggplant genotypes were capable of using their antioxidant enzyme systems more actively than the salt-sensitive ones.

References

  • Asada K (1994). Mechanisms for scavenging reactive molecules generated in chloroplast under light stress. In: Baker, N.R and J. R. Bower, eds. Bios Scientific Publishers, Oxford, pp. 131–145. Asada K , Takahashi M (1987). Production and scavenging of active oxygen radicals in photosynthesis. In: D.J.Kyle et al. (Eds.) Photoinhibition. Elsevier, Amsterdam, 227-297.
  • Blokhina O, Virolainen E, Fagerstedt KV (2003). Antioxidants, oxidative damage and oxygen deprivation stress: a review. Annals of Botany, 91: 179-194.
  • Bowler C, Montagu MV, Inze D (1992). Superoxide dismutase and stress tolerance. Annu. Rev. Plant Mol. Biol. 43: 83-116.
  • Cakmak I, Marschner H (1992). Magnesium defficiency and highlight intensity enhance activities of superoxide dismutase, ascorbate peroxidase and glutathione reductase in bean leaves. Plant Physiol. 98: 1222-1226.
  • Cakmak I, Strbac D, Marschner H (1993). Activities of hydrogen peroxide-scavenging enzymes in germinating wheat seeds. J. of Exp. Bot. 44: 127-132.
  • Cakmak I (1994). Activity of ascorbate-dependent H 2 2 scavenging enzymes and leaf chlorosis are enhanced in magnesium and potassium deficient leaves, but not in phosphorus deficient leaves. J. Exp. Bot. 45: 1259-1266.
  • Dhindsa RS, Mathowe W (1981). Drought tolerance in two mosses: correlated with enzymatic defence against lipid peroxidation. J. of Exp. Bot. 32 (126): 79-91.
  • Foyer CH, Lendais M, Kunert KJ (1994). Photooxidative stress in plants. Phsiol. Plant. 92: 696-717. Gossett DR, Millhollon EP, Lucas MC (1994 a). Antioxidant response to NaCl stress in salt-tolerant and salt-sensitive cultivars of cotton. Crop Sci. 34: 706-714.
  • Gossett DR, Millhollon EP, Lucas MC, Banks SW, Marney MM (1994 b). The effects of NaCl on antioxidant activities in callus tissue of salt-sensitive cotton cultivars (Gosspium hirsitum L.). Plant Cell Reports 13: 498-503.
  • Halliwell B, Gutteridge JMC (1989). Protection against oxidants in biological systems: The super oxide theory of oxygen toxicity. In: Halliwell B, Gutteridge JMC (eds.) Free Radicals in Biology and Medicine. Clarendon Press, Oxford, pp 86–123.
  • Hernandez JA, Del Rio IA, Sevilla F (1994). Salt stress-induced changes in superoxide dismutase isozymes in leaves and mesophyll protoplasts from Vigna unguiculata (L.) Walp. New Phytol. 126: 37-44.
  • Hernandez JA, Olmos E, Corpas FJ, Sevilla F, Del Rio IA (1995). Salt-induced oxidative stress in chloroplasts of pea plants. Plant Sci. 105: 151-167.
  • Hoagland DR, Arnon DI (1938). The water culture method for growing plants without soil. Circ. Calif. Agr. Exp. Sta. 347-461.
  • Jiang M, Zhang J (2002). Water stress-induced abscisic acid accumulation triggers the increased generation of reactive oxygen species and up-regulates the activities of antioxidant enzymes in maize leaves. J. Exp. Bot. 53: 2401–2410.
  • Karanlık S (2001). Resistance to salinity in different wheat genotypes and physiological mechanisms involved in salt resistance. Ph.D. Thesis, Institute of Natural and Applied Science, University of Cukurova. Turkey, 122 p.(in Turkish).
  • Lin CC, Kao CH (2000). Effect of NaCl Stress on H 2 O 2 metabolism in rice leaves. Plant Growth Regul. 30: 151-155.
  • Lingqiang MG, Scandalios JG (2002). Catalase gene expression in response to auxin-mediated developmental signals. Physiol. Plant. 114: 288–295.
  • Lopez MV, Satti SME (1996). Calcium and Potassium- Enhanced Growth and Yield of Tomato Under Sodium Chloride Stress. Plant Sci. 114: 19-27.
  • Okuda T, Matsuda Y, Yamanaka A, Sagisaka S (1991). Abrupt increase in the level of hydrogen peroxide in leaves of wheat is caused by cold treatment. J.Plant Physiol. 97: 1265-1267.
  • SAS (1988). SAS/State User’s Guide. 6.03 Edition. SAS Institute, Cary, NC, USA.
  • Scandalio LM, Dalurzo HC, Gómez M, Romero-Puertas MC, Del Rio LA (2001). Cadmium-induced changes in the growth and oxidative metabolism of pea plants. J. Exp. Bot. 52: 2115–2126. Shalata A, Tal M (1998). The effect of salt stress on lipid peroxidation avd antioxidants in the leaf of the cultivated tomato and its wild salt-tolerant relative Lycopersicon pennellii. Physiol. Plant. 104: 169-17
  • Shalata A, Mittova V, Volokita M, Guy M, Tal M (2001). Response of the cultivated tomato and its wild salt-tolerant relative Lycopersicon pennellii to salt-dependent oxidative stress: The root antioxidative system, Physiol. Plant. 112: 487–494.
  • Silvana BD, Gallego SM, Benavides MP, Tomaro ML ( 2003). Behaviour of antioxidant defense system in the adaptive response to salt stress in Helianthus annuus L. Cells. Plant Growth Regulation 40: 81-88.
  • Spychalla JP, Desborough SL (1990). Superoxide dismutase, catalase, and alph-tocopherol conetnt of stored potato tubers. Plant Physiol. 94: 1214-1218.
  • Sreenivasulu N, Grimm B, Wobus U, Weschke W (2000). Differential response of antioxidant compounds to salinity stress in salt-tolerant and salt-sensitive seedling of fox-tail millet (Setaria italica). Physiol. Plant. 109: 435-442.
  • Tsugane K, Kobayashi K, Niwa Y, Ohba Y, Wada K, Kobayashi H (1999). A recessive Arabidopsis mutant that grows photoautotrophically under salt stress shows enhanced active oxygen detoxification. Plant Cell 11(7): 1195–1206.
  • Vaidyanathan H, Sivakumar P, Chakrabarty R, Thomas G (2003). Scavenging of reactive oxygen species in NaCl-stressed rice (Oryza sativa L.) differential response in salt-tolerant and sensitive varieties. Plant Science 165: 1411–1418.
  • Wise RR, Naylor AW (1987). Chilling-Enhanced Photooxidation: evidence for the role of singlet oxygen and endogenous antioxidants. Plant Physiol. 83: 278-282.
  • Yaşar F (2003). Investigation of some antioxidant enzyme activities in eggplant genotypes grown under salt stress in vitro and in vivo. Ph.D. Thesis, Institute of Natural and Applied Science, University of Yuzuncu Yil, Turkey, 139 pp (in Turkish).
  • Yaşar F, Ellialtioğlu Ş (2008). Tuz Stresi Altında Yetiştirilen Patlıcan Genotiplerinde Meydana Gelen Morfolojik, Fizyolojik ve Biyokimyasal Değişimler. Yüzüncü Yıl Üniversitesi, Fen Bilimleri Enstitüsü Dergisi, 13 (1): 51-68.

Antioxidative Responses of Some Eggplant Genotypes to Salinity Stress

Year 2013, Volume: 23 Issue: 3, 215 - 221, 01.09.2013

Abstract

Patlıcanda antioksidant enzimlerin tuz stresine olan tepkileri çalışılmıştır. Çalışmada, Türkiyenin farklı bölgelerinde yetiştiriciliği yapılan Solanum melongena L türüne ait tuza tolerant olan Burdur Bucak (BB) ve Mardin Kızıltepe (MK) genotipleri ile tuza hassas Giresun (Gi) ve Artvin Hopa (AH) genotipleri ve yabani bir tür olan S. sisymbriifolium (SS) kullanılmıştır. Hidrofonik kültür ortamında tuz stresi altında genotiplerin, süperoksit dismutaz (SOD; EC 1.15.1.1), katalaz (CAT; EC 1.11.1.6), askorbat peroksidaz (APX; EC 1.1.1.11) ve glutatyon reduktaz (GR; EC 1.6.4.2) enzim aktiviteleri incelenmiştir. Araştırma sonucunda, antioksidant enzim aktivitelerinin tuza tolerans üzerinde çok etkili olduğu; tuzlu koşullarda yaşayabilen patlıcan genotiplerinin antioksidatif enzim sistemlerini duyarlı genotiplere göre çok daha aktif kullandıkları belirlenmiştir.

References

  • Asada K (1994). Mechanisms for scavenging reactive molecules generated in chloroplast under light stress. In: Baker, N.R and J. R. Bower, eds. Bios Scientific Publishers, Oxford, pp. 131–145. Asada K , Takahashi M (1987). Production and scavenging of active oxygen radicals in photosynthesis. In: D.J.Kyle et al. (Eds.) Photoinhibition. Elsevier, Amsterdam, 227-297.
  • Blokhina O, Virolainen E, Fagerstedt KV (2003). Antioxidants, oxidative damage and oxygen deprivation stress: a review. Annals of Botany, 91: 179-194.
  • Bowler C, Montagu MV, Inze D (1992). Superoxide dismutase and stress tolerance. Annu. Rev. Plant Mol. Biol. 43: 83-116.
  • Cakmak I, Marschner H (1992). Magnesium defficiency and highlight intensity enhance activities of superoxide dismutase, ascorbate peroxidase and glutathione reductase in bean leaves. Plant Physiol. 98: 1222-1226.
  • Cakmak I, Strbac D, Marschner H (1993). Activities of hydrogen peroxide-scavenging enzymes in germinating wheat seeds. J. of Exp. Bot. 44: 127-132.
  • Cakmak I (1994). Activity of ascorbate-dependent H 2 2 scavenging enzymes and leaf chlorosis are enhanced in magnesium and potassium deficient leaves, but not in phosphorus deficient leaves. J. Exp. Bot. 45: 1259-1266.
  • Dhindsa RS, Mathowe W (1981). Drought tolerance in two mosses: correlated with enzymatic defence against lipid peroxidation. J. of Exp. Bot. 32 (126): 79-91.
  • Foyer CH, Lendais M, Kunert KJ (1994). Photooxidative stress in plants. Phsiol. Plant. 92: 696-717. Gossett DR, Millhollon EP, Lucas MC (1994 a). Antioxidant response to NaCl stress in salt-tolerant and salt-sensitive cultivars of cotton. Crop Sci. 34: 706-714.
  • Gossett DR, Millhollon EP, Lucas MC, Banks SW, Marney MM (1994 b). The effects of NaCl on antioxidant activities in callus tissue of salt-sensitive cotton cultivars (Gosspium hirsitum L.). Plant Cell Reports 13: 498-503.
  • Halliwell B, Gutteridge JMC (1989). Protection against oxidants in biological systems: The super oxide theory of oxygen toxicity. In: Halliwell B, Gutteridge JMC (eds.) Free Radicals in Biology and Medicine. Clarendon Press, Oxford, pp 86–123.
  • Hernandez JA, Del Rio IA, Sevilla F (1994). Salt stress-induced changes in superoxide dismutase isozymes in leaves and mesophyll protoplasts from Vigna unguiculata (L.) Walp. New Phytol. 126: 37-44.
  • Hernandez JA, Olmos E, Corpas FJ, Sevilla F, Del Rio IA (1995). Salt-induced oxidative stress in chloroplasts of pea plants. Plant Sci. 105: 151-167.
  • Hoagland DR, Arnon DI (1938). The water culture method for growing plants without soil. Circ. Calif. Agr. Exp. Sta. 347-461.
  • Jiang M, Zhang J (2002). Water stress-induced abscisic acid accumulation triggers the increased generation of reactive oxygen species and up-regulates the activities of antioxidant enzymes in maize leaves. J. Exp. Bot. 53: 2401–2410.
  • Karanlık S (2001). Resistance to salinity in different wheat genotypes and physiological mechanisms involved in salt resistance. Ph.D. Thesis, Institute of Natural and Applied Science, University of Cukurova. Turkey, 122 p.(in Turkish).
  • Lin CC, Kao CH (2000). Effect of NaCl Stress on H 2 O 2 metabolism in rice leaves. Plant Growth Regul. 30: 151-155.
  • Lingqiang MG, Scandalios JG (2002). Catalase gene expression in response to auxin-mediated developmental signals. Physiol. Plant. 114: 288–295.
  • Lopez MV, Satti SME (1996). Calcium and Potassium- Enhanced Growth and Yield of Tomato Under Sodium Chloride Stress. Plant Sci. 114: 19-27.
  • Okuda T, Matsuda Y, Yamanaka A, Sagisaka S (1991). Abrupt increase in the level of hydrogen peroxide in leaves of wheat is caused by cold treatment. J.Plant Physiol. 97: 1265-1267.
  • SAS (1988). SAS/State User’s Guide. 6.03 Edition. SAS Institute, Cary, NC, USA.
  • Scandalio LM, Dalurzo HC, Gómez M, Romero-Puertas MC, Del Rio LA (2001). Cadmium-induced changes in the growth and oxidative metabolism of pea plants. J. Exp. Bot. 52: 2115–2126. Shalata A, Tal M (1998). The effect of salt stress on lipid peroxidation avd antioxidants in the leaf of the cultivated tomato and its wild salt-tolerant relative Lycopersicon pennellii. Physiol. Plant. 104: 169-17
  • Shalata A, Mittova V, Volokita M, Guy M, Tal M (2001). Response of the cultivated tomato and its wild salt-tolerant relative Lycopersicon pennellii to salt-dependent oxidative stress: The root antioxidative system, Physiol. Plant. 112: 487–494.
  • Silvana BD, Gallego SM, Benavides MP, Tomaro ML ( 2003). Behaviour of antioxidant defense system in the adaptive response to salt stress in Helianthus annuus L. Cells. Plant Growth Regulation 40: 81-88.
  • Spychalla JP, Desborough SL (1990). Superoxide dismutase, catalase, and alph-tocopherol conetnt of stored potato tubers. Plant Physiol. 94: 1214-1218.
  • Sreenivasulu N, Grimm B, Wobus U, Weschke W (2000). Differential response of antioxidant compounds to salinity stress in salt-tolerant and salt-sensitive seedling of fox-tail millet (Setaria italica). Physiol. Plant. 109: 435-442.
  • Tsugane K, Kobayashi K, Niwa Y, Ohba Y, Wada K, Kobayashi H (1999). A recessive Arabidopsis mutant that grows photoautotrophically under salt stress shows enhanced active oxygen detoxification. Plant Cell 11(7): 1195–1206.
  • Vaidyanathan H, Sivakumar P, Chakrabarty R, Thomas G (2003). Scavenging of reactive oxygen species in NaCl-stressed rice (Oryza sativa L.) differential response in salt-tolerant and sensitive varieties. Plant Science 165: 1411–1418.
  • Wise RR, Naylor AW (1987). Chilling-Enhanced Photooxidation: evidence for the role of singlet oxygen and endogenous antioxidants. Plant Physiol. 83: 278-282.
  • Yaşar F (2003). Investigation of some antioxidant enzyme activities in eggplant genotypes grown under salt stress in vitro and in vivo. Ph.D. Thesis, Institute of Natural and Applied Science, University of Yuzuncu Yil, Turkey, 139 pp (in Turkish).
  • Yaşar F, Ellialtioğlu Ş (2008). Tuz Stresi Altında Yetiştirilen Patlıcan Genotiplerinde Meydana Gelen Morfolojik, Fizyolojik ve Biyokimyasal Değişimler. Yüzüncü Yıl Üniversitesi, Fen Bilimleri Enstitüsü Dergisi, 13 (1): 51-68.
There are 30 citations in total.

Details

Primary Language Turkish
Journal Section Articles
Authors

F. Yaşar This is me

Ş. Ellialtıoğlu This is me

Publication Date September 1, 2013
Published in Issue Year 2013 Volume: 23 Issue: 3

Cite

APA Yaşar, F., & Ellialtıoğlu, Ş. (2013). Antioxidative Responses of Some Eggplant Genotypes to Salinity Stress. Yuzuncu Yıl University Journal of Agricultural Sciences, 23(3), 215-221.
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Yuzuncu Yil University Journal of Agricultural Sciences by Van Yuzuncu Yil University Faculty of Agriculture is licensed under a Creative Commons Attribution 4.0 International License.