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Van Gölü Havzası Kavunlarındaki Moleküler Genetik Çeşitliliğin RAPD ve ISSR Belirteçleri İle Belirlenmesi

Year 2013, Volume: 23 Issue: 3, 264 - 270, 01.09.2013

C. Erdınc A. Ekıncıalp M. Yıldız T. Kabay O. Turkmen S. Sensoy

Abstract

Lake Van Basin of Turkey is located in the secondary gene center for melon. The molecular genetic diversity among thirty-seven melon genotypes collected from Lake Van Basin was determined by RAPD and ISSR makers. Total 121 polymorphic molecular markers obtained from 8 RAPD and 10 ISSR primers were employed to characterize the genetic relationships among the melon genotypes. It was found that within-group genetic similarities ranged between 0.55 and 1.00; related genotypes or genotypes collected from similar regions were partitioned to similar clusters. The genetic diversity parameters among Lake Van Basin melon genotypes were found as H = 0.175 and I = 0.231, and 96.19 % of polymorphism.

Keywords

Cucumis melo L. Genetic variation Germplasm RAPD ISSR

References

  • Anonymous (2010) FAOSTAT. Statistic Database. http://faostat.fao.org
  • Chen Y. G. Li X.L. Wang (2010). Genetic diversity of a germplasm collection of Cucumis melo L. using SRAP markers. Hereditas 32 (7), 744-751.
  • Demir S. Ö. Türkmen S. Sensoy, A. Akköprü, C. Erdinc, M. Yıldız, and T. Kabay (2006). Reactions of melon landraces grown in the lake Van Basin to the physiological races of Fusarium oxysporum f.sp. melonis. Eur. J. Hort. Sci.71(2):91-95.
  • Dje Y. G.C. Tahi, I.A. Zoro Bi, M. Malice, J.P. Baudoin and P. Bertin (2006). Optimization of ISSR marker for African edible-seeded Cucurbitaceae species genetic diversity analysis. African Journal of Biotechnology 5(2), 83-87.
  • Dje Y. G.C. Tahi, I.A. Zoro Bi, M. Malice, J.P. Baudoin and P. Bertin (2010). Use of ISSR markers to assess genetic diversity of African edible seeded Citrullus lanatus landraces. Scientia Horticulturae 124, 159-164.
  • Doyle J.J. and J.L. Doyle (1987). A rapid DNA isolation procedure from small quantities of fresh leaf tissues. Phytochemical Bulletin 19, 11-15.
  • Ekbic E, H. Fidan, M. Yildiz, and K. Abak (2010). Screening of Turkish melon accessions for resistance to ZYMV, WMV and CMV. Notulae Scientia Biologicae 2(1), 55-57.
  • Garcia E, M. Jamilena, J.I. Alvarez, T. Arnedo, J.L. Oliver and R. Lozano (1998). Genetic relationships among melon breeding lines revealed by RAPD markers and agronomic traits. Theoretical and Applied Genetics 96, 878-885.
  • Garcia-Mas J, M. Oliver, H. Gomez-Paniagua and M.C. de Vivente (2000). Comparing AFLP, RAPD and RFLP markers for measuring genetic diversity in melon. Theoretical and Applied Genetics 101, 860-86
  • Gilbert J.E, R.V. Lewis, M.J. Wilkinson and P.D.S. Caligari (1999). Developing an appropriate strategy to assess genetic variability in germplasm collection. Theoretical and Applied Genetics 98, 112511
  • Günay A, 1993. Vegetable Production V. A.Ü., Ziraat Fak., Ankara (in Turkish).
  • Inan N, M. Yildiz, S. Sensoy, S. Kafkas and K. Abak (2012). Efficacy of ISSR and SRAP Techniques for Molecular Characterization of Some Cucurbita Genotypes Including Naked (Hull-Less) Seed Pumpkin. The Journal of Animal & Plant Sciences. 22(1):126-136.
  • Jeffrey C (2001). Cucurbitacae. In: Hanelt P. and Institute of Plant Genetics and Crop Plant Research (eds.), Mansfeld’s Encyclopedia of Agricultural and Horticultural Crops. Springer, New York, NY, USA.
  • Kucuk A, K. Abak and N. Sari (2002). Cucurbit genetic resources collections in Turkey. First AD HOC Meeting on Cucurbit Genetic Resources. 19 January 2002, Adana, Turkey 46-51.
  • Kusvuran S, H.Y. Dasgan, K. Abak (2011). Responses of Different Melon Genotypes to Drought Stress. YYU J Agr Sci 21 (3), 209-219.
  • Lee M (1995). DNA markers and plant breeding programs. Advances in Agronomy 55, 265-344.
  • Lopez-Sese A., J.E. Staub, N. Katzir and M.L. Gomez-Guillamon (2002). Estimation of between and within accession variation in selected Spanish melon germplasm using RAPD and SSR markers to assess strategies for large collection evaluation. Euphytica 127 (1), 41-51.
  • Luan F I. Delannay and J.E. Staub (2008). Chinese melon (Cucumis melo L.) diversity analyses provide strategies for germplasm curation, genetic improvement, and evidentiary support of domestication patterns. Euphytica 164, 445-461.
  • Meglic V. and J.E. Staub (1996). Inheritance and linkage relationships of isozyme and morphological loci in cucumber (Cucumis sativus L.). Theoretical and Applied Genetics 92, 865-872.
  • Mliki A, J.E. Staub, S. Zhangyong and A. Ghorbel (2001). Genetic diversity in melon (Cucumis melo L.): An evaluation of African germplasm. Genetic Resources Crop Evolution 48, 587-597.
  • Nakata E, J.E. Staub, A.I. Lopez-Sese and N. Katzir (2005). Genetic diversity of Japanese melon cultivars (Cucumis melo L.) as assessed by random amplified polymorphic DNA and simple sequence repeat markers. Genetic Resources Crop Evolution 52 (4), 405-419.
  • Nei M (1973). Analysis of gene diversity in subdivided populations. Proceeding of the National Academy of Sciences of the USA 70, 3321-3323.
  • Nhi P.T.P, Y. Akashi, T.T.M. Hang, K. Tanaka, Y. Aierken, T. Yamamoto, H. Nishida, C. Long and K. Kato (2010). Genetic diversity in Vietnames melon landraces revealed by the analyses of morphological traits and nuclear and cytoplasmic molecular markers. Breeding Science 60, 25526
  • Nimmakayala P, Y.R. Tomason, J. Jeong, G. Vajja, A. Levi, P. Gibson and U.K. Reddy (2008). Molecular diversity in the Ukrainian melon collection as revealed by AFLPs and microsatellites. Plant Genetic Resources 7(2), 127-134.
  • Pitrat M, M. Chauvet and C. Foury (1999). Diversity, history and production of cultivated cucurbits. Proc I st IS on Cucurbits (Eds K Abak and S Büyükalaca) Acta Horticulturae 492, 21-28.
  • Pitrat M, P. Hanelt and K. Hammer (2000). Some comments on intraspecific classification of cultivars of melon. Acta Horticulturae 510, 29-36.
  • Rafalski J.A. and S.V. Tingey (1993). Genetic diagnostics in plant breeding: RAPDs, microsatellites and machines. Trends in Genetics 9 (8), 275-280.
  • Robinson R.W. and D.S. Decker-Walters (1997). Cucurbits. CAB Int. University Press, Cambridge. p: 2
  • Rohlf F.J (1997). Ntsys-Pc: Numerical taxonomy and multivariate analysis system. Exeter Software, New York.
  • Sari N, A. Tan, R. Yanmaz, H. Yetisir, A. Balkaya, I. Solmaz, L. Aykas and M. Pitrat (2008). General status of cucurbit genetic resources in Turkey. Proc of the IX th EUCARPIA meeting on genetics and breeding of Cucurbitaceae (Ed Pitrat M), Avignon (France) May 21-24 th pp: 21-32.
  • Sensoy S, S Buyukalaca and K. Abak (2007a). Evaluation of genetic diversity in Turkish melons (Cucumis melo L.) based on phenotypic characters and RAPD markers. Genetic Resources Crop Evolution 54 (6), 1351-1365.
  • Sensoy S, S. Demir, S. Buyukalaca and K. Abak (2007b). Response of Turkish melon genotypes to Fusarium oxysporum f. sp. melonis race 1 determined by inoculation tests and RAPD markers. European Journal Horticultural Science 72 (5), 220-227.
  • Sestili S, A. Daniele, A. Rosa, V. Ferrari, A. Belisario and N. Ficcadenti (2008). Molecular characterization of different Italian inodorus melon populations based on ISSR molecular markers and preliminary SSR analysis. Proc of the IX th EUCARPIA meeting on genetics and breeding of Cucurbitaceae, Avignon (France) May 21-24 th pp: 307-312.
  • Shannon C.E and W. Weaver (1949). The mathematical Theory of Communication. Univ of Illinois Press, Urbana.
  • Sheng Y, F. Luan, K. Chen, X. Cui and J.E. Staub (2007). Genetic diversity of Chinese thin-skinned melon cultivars (Cucumis melo L.) based on simple sequence repeat markers. Acta Horticulturae 763, 169-176.
  • Silberstein L, I. Kovalski, R. Huang, K. Anagnostou, M.M.K. Jahn and R. Perl-Treves (1999). Molecular variation in melon (Cucumis melo L.) as revealed by RFLP and RAPD markers. Scientia Horticulturae 79, 101-111.
  • Sokal R.R and P.H.A. Sneath1963. Principles of Numerical Taxonomy. Freeman. San Francisco, pp: 359. Soltani F, Y. Akashi, A. Kashi, Z. Zamani and Y. Mostofi (2010). Characterization of Iranian melon landraces of Cucumis melo L. groups flexuosus and dudaim by analysis of morphological characters and random amplified polymorphic DNA. Breeding Science 60, 34-45.
  • Staub J.E, A.I. Lopez-Sese and N. Fanourakis (2004). Diversity among melon landraces (Cucumis melo L.) from Greece and their genetic relationships with other melon germplasm of diverse origins. Euphytica 136, 151-166.
  • Szamosi C, I. Solmaz, N. Sari, C. Barsony (2010). Morphological evaluation and comparison of Hungarian and Turkish melon (Cucumis melo L.) germplasm. Scientia Horticulturae 124, 17018
  • Waugh R and W. Powell (1992). Using RAPD markers for crop improvement. Trends in Biotechnology 10, 186-191.
  • Winter P and G. Kahl (1995). Molecular marker technologies for plant improvement. World Journal of Microbiology and Biotechnology 11, 438-448.
  • Yeh F.C, R.C. Yang, T. Boiley, Z.H. Ye and J.X. Mao (1997). POPGENE, The user-friendly shareware for population genetic analysis. Molecular Biology and Biotechnology Center, University of Alberta, Canada.
  • Yi S.S, Y. Akashi, K. Tanaka, T.T. Cho, M.T. Khaing, H. Yoshino, H. Nishida, T. Yamamoto, K. Win and K. Kato (2009). Molecular analsis of genetic diversity in melon landraces (Cucumis melo L.) from Myanmar and their relationship with melon germplasm from East and South Asia. Genetic Resources Crop Evolution 56, 1149-1161.
  • Yildiz M, E. Ekbic, D. Keles, S. Sensoy and K. Abak (2011). Use of ISSR, SRAP, and RAPD markers to assess genetic diversity in Turkish melons. Scientia Horticulturae 130 (2011) 349–353.
  • Zhukovsky P (1951). Agricultural Structure of Turkey (Anatolia). Türkiye Şeker Fab. AŞ. 1951. Yay No: 887 s. (in Turkish).

Molecular Genetic Diversity in Lake Van Basin Melons (Cucumis melo L.) Based on RAPD and ISSR Markers

Year 2013, Volume: 23 Issue: 3, 264 - 270, 01.09.2013

C. Erdınc A. Ekıncıalp M. Yıldız T. Kabay O. Turkmen S. Sensoy

Abstract

Türkiye Van Gölü Havzası kavunun ikincil gen merkezinde yer almaktadır. Van Gölü Havzası’ndan toplanan otuz yedi kavun genotipi arasındaki moleküler genetik çeşitlilik, RAPD ve ISSR belirteçleri yardımıyla belirlenmiştir. Sekiz adet RAPD ve 10 adet ISSR primerinden elde edilen toplam 121 polimorfik moleküler belirteç, kavun genotipleri arasındaki genetik ilişkilerin karakterizasyonunda kullanılmıştır. Grup içi genetik benzerlik katsayısının 0.55 ve 1.00 arasında değiştiği; benzer bölgelerden toplanan genotiplerin veya akraba genotiplerin benzer kümelerde yer aldığı gözlenmiştir. Van Gölü Havzası kavun genotipler arasında genetik çeşitlilik parametreleri olarak H = 0.175, I = 0.231 ve % 96.19 polimorfizm belirlenmiştir.

Keywords

Cucumis melo L. Genetik varyasyon Gen kaynakları RAPD ISSR

References

  • Anonymous (2010) FAOSTAT. Statistic Database. http://faostat.fao.org
  • Chen Y. G. Li X.L. Wang (2010). Genetic diversity of a germplasm collection of Cucumis melo L. using SRAP markers. Hereditas 32 (7), 744-751.
  • Demir S. Ö. Türkmen S. Sensoy, A. Akköprü, C. Erdinc, M. Yıldız, and T. Kabay (2006). Reactions of melon landraces grown in the lake Van Basin to the physiological races of Fusarium oxysporum f.sp. melonis. Eur. J. Hort. Sci.71(2):91-95.
  • Dje Y. G.C. Tahi, I.A. Zoro Bi, M. Malice, J.P. Baudoin and P. Bertin (2006). Optimization of ISSR marker for African edible-seeded Cucurbitaceae species genetic diversity analysis. African Journal of Biotechnology 5(2), 83-87.
  • Dje Y. G.C. Tahi, I.A. Zoro Bi, M. Malice, J.P. Baudoin and P. Bertin (2010). Use of ISSR markers to assess genetic diversity of African edible seeded Citrullus lanatus landraces. Scientia Horticulturae 124, 159-164.
  • Doyle J.J. and J.L. Doyle (1987). A rapid DNA isolation procedure from small quantities of fresh leaf tissues. Phytochemical Bulletin 19, 11-15.
  • Ekbic E, H. Fidan, M. Yildiz, and K. Abak (2010). Screening of Turkish melon accessions for resistance to ZYMV, WMV and CMV. Notulae Scientia Biologicae 2(1), 55-57.
  • Garcia E, M. Jamilena, J.I. Alvarez, T. Arnedo, J.L. Oliver and R. Lozano (1998). Genetic relationships among melon breeding lines revealed by RAPD markers and agronomic traits. Theoretical and Applied Genetics 96, 878-885.
  • Garcia-Mas J, M. Oliver, H. Gomez-Paniagua and M.C. de Vivente (2000). Comparing AFLP, RAPD and RFLP markers for measuring genetic diversity in melon. Theoretical and Applied Genetics 101, 860-86
  • Gilbert J.E, R.V. Lewis, M.J. Wilkinson and P.D.S. Caligari (1999). Developing an appropriate strategy to assess genetic variability in germplasm collection. Theoretical and Applied Genetics 98, 112511
  • Günay A, 1993. Vegetable Production V. A.Ü., Ziraat Fak., Ankara (in Turkish).
  • Inan N, M. Yildiz, S. Sensoy, S. Kafkas and K. Abak (2012). Efficacy of ISSR and SRAP Techniques for Molecular Characterization of Some Cucurbita Genotypes Including Naked (Hull-Less) Seed Pumpkin. The Journal of Animal & Plant Sciences. 22(1):126-136.
  • Jeffrey C (2001). Cucurbitacae. In: Hanelt P. and Institute of Plant Genetics and Crop Plant Research (eds.), Mansfeld’s Encyclopedia of Agricultural and Horticultural Crops. Springer, New York, NY, USA.
  • Kucuk A, K. Abak and N. Sari (2002). Cucurbit genetic resources collections in Turkey. First AD HOC Meeting on Cucurbit Genetic Resources. 19 January 2002, Adana, Turkey 46-51.
  • Kusvuran S, H.Y. Dasgan, K. Abak (2011). Responses of Different Melon Genotypes to Drought Stress. YYU J Agr Sci 21 (3), 209-219.
  • Lee M (1995). DNA markers and plant breeding programs. Advances in Agronomy 55, 265-344.
  • Lopez-Sese A., J.E. Staub, N. Katzir and M.L. Gomez-Guillamon (2002). Estimation of between and within accession variation in selected Spanish melon germplasm using RAPD and SSR markers to assess strategies for large collection evaluation. Euphytica 127 (1), 41-51.
  • Luan F I. Delannay and J.E. Staub (2008). Chinese melon (Cucumis melo L.) diversity analyses provide strategies for germplasm curation, genetic improvement, and evidentiary support of domestication patterns. Euphytica 164, 445-461.
  • Meglic V. and J.E. Staub (1996). Inheritance and linkage relationships of isozyme and morphological loci in cucumber (Cucumis sativus L.). Theoretical and Applied Genetics 92, 865-872.
  • Mliki A, J.E. Staub, S. Zhangyong and A. Ghorbel (2001). Genetic diversity in melon (Cucumis melo L.): An evaluation of African germplasm. Genetic Resources Crop Evolution 48, 587-597.
  • Nakata E, J.E. Staub, A.I. Lopez-Sese and N. Katzir (2005). Genetic diversity of Japanese melon cultivars (Cucumis melo L.) as assessed by random amplified polymorphic DNA and simple sequence repeat markers. Genetic Resources Crop Evolution 52 (4), 405-419.
  • Nei M (1973). Analysis of gene diversity in subdivided populations. Proceeding of the National Academy of Sciences of the USA 70, 3321-3323.
  • Nhi P.T.P, Y. Akashi, T.T.M. Hang, K. Tanaka, Y. Aierken, T. Yamamoto, H. Nishida, C. Long and K. Kato (2010). Genetic diversity in Vietnames melon landraces revealed by the analyses of morphological traits and nuclear and cytoplasmic molecular markers. Breeding Science 60, 25526
  • Nimmakayala P, Y.R. Tomason, J. Jeong, G. Vajja, A. Levi, P. Gibson and U.K. Reddy (2008). Molecular diversity in the Ukrainian melon collection as revealed by AFLPs and microsatellites. Plant Genetic Resources 7(2), 127-134.
  • Pitrat M, M. Chauvet and C. Foury (1999). Diversity, history and production of cultivated cucurbits. Proc I st IS on Cucurbits (Eds K Abak and S Büyükalaca) Acta Horticulturae 492, 21-28.
  • Pitrat M, P. Hanelt and K. Hammer (2000). Some comments on intraspecific classification of cultivars of melon. Acta Horticulturae 510, 29-36.
  • Rafalski J.A. and S.V. Tingey (1993). Genetic diagnostics in plant breeding: RAPDs, microsatellites and machines. Trends in Genetics 9 (8), 275-280.
  • Robinson R.W. and D.S. Decker-Walters (1997). Cucurbits. CAB Int. University Press, Cambridge. p: 2
  • Rohlf F.J (1997). Ntsys-Pc: Numerical taxonomy and multivariate analysis system. Exeter Software, New York.
  • Sari N, A. Tan, R. Yanmaz, H. Yetisir, A. Balkaya, I. Solmaz, L. Aykas and M. Pitrat (2008). General status of cucurbit genetic resources in Turkey. Proc of the IX th EUCARPIA meeting on genetics and breeding of Cucurbitaceae (Ed Pitrat M), Avignon (France) May 21-24 th pp: 21-32.
  • Sensoy S, S Buyukalaca and K. Abak (2007a). Evaluation of genetic diversity in Turkish melons (Cucumis melo L.) based on phenotypic characters and RAPD markers. Genetic Resources Crop Evolution 54 (6), 1351-1365.
  • Sensoy S, S. Demir, S. Buyukalaca and K. Abak (2007b). Response of Turkish melon genotypes to Fusarium oxysporum f. sp. melonis race 1 determined by inoculation tests and RAPD markers. European Journal Horticultural Science 72 (5), 220-227.
  • Sestili S, A. Daniele, A. Rosa, V. Ferrari, A. Belisario and N. Ficcadenti (2008). Molecular characterization of different Italian inodorus melon populations based on ISSR molecular markers and preliminary SSR analysis. Proc of the IX th EUCARPIA meeting on genetics and breeding of Cucurbitaceae, Avignon (France) May 21-24 th pp: 307-312.
  • Shannon C.E and W. Weaver (1949). The mathematical Theory of Communication. Univ of Illinois Press, Urbana.
  • Sheng Y, F. Luan, K. Chen, X. Cui and J.E. Staub (2007). Genetic diversity of Chinese thin-skinned melon cultivars (Cucumis melo L.) based on simple sequence repeat markers. Acta Horticulturae 763, 169-176.
  • Silberstein L, I. Kovalski, R. Huang, K. Anagnostou, M.M.K. Jahn and R. Perl-Treves (1999). Molecular variation in melon (Cucumis melo L.) as revealed by RFLP and RAPD markers. Scientia Horticulturae 79, 101-111.
  • Sokal R.R and P.H.A. Sneath1963. Principles of Numerical Taxonomy. Freeman. San Francisco, pp: 359. Soltani F, Y. Akashi, A. Kashi, Z. Zamani and Y. Mostofi (2010). Characterization of Iranian melon landraces of Cucumis melo L. groups flexuosus and dudaim by analysis of morphological characters and random amplified polymorphic DNA. Breeding Science 60, 34-45.
  • Staub J.E, A.I. Lopez-Sese and N. Fanourakis (2004). Diversity among melon landraces (Cucumis melo L.) from Greece and their genetic relationships with other melon germplasm of diverse origins. Euphytica 136, 151-166.
  • Szamosi C, I. Solmaz, N. Sari, C. Barsony (2010). Morphological evaluation and comparison of Hungarian and Turkish melon (Cucumis melo L.) germplasm. Scientia Horticulturae 124, 17018
  • Waugh R and W. Powell (1992). Using RAPD markers for crop improvement. Trends in Biotechnology 10, 186-191.
  • Winter P and G. Kahl (1995). Molecular marker technologies for plant improvement. World Journal of Microbiology and Biotechnology 11, 438-448.
  • Yeh F.C, R.C. Yang, T. Boiley, Z.H. Ye and J.X. Mao (1997). POPGENE, The user-friendly shareware for population genetic analysis. Molecular Biology and Biotechnology Center, University of Alberta, Canada.
  • Yi S.S, Y. Akashi, K. Tanaka, T.T. Cho, M.T. Khaing, H. Yoshino, H. Nishida, T. Yamamoto, K. Win and K. Kato (2009). Molecular analsis of genetic diversity in melon landraces (Cucumis melo L.) from Myanmar and their relationship with melon germplasm from East and South Asia. Genetic Resources Crop Evolution 56, 1149-1161.
  • Yildiz M, E. Ekbic, D. Keles, S. Sensoy and K. Abak (2011). Use of ISSR, SRAP, and RAPD markers to assess genetic diversity in Turkish melons. Scientia Horticulturae 130 (2011) 349–353.
  • Zhukovsky P (1951). Agricultural Structure of Turkey (Anatolia). Türkiye Şeker Fab. AŞ. 1951. Yay No: 887 s. (in Turkish).
There are 45 citations in total.

Details

Primary Language Turkish
Journal Section Articles
Authors

C. Erdınc This is me

A. Ekıncıalp This is me

M. Yıldız This is me

T. Kabay This is me

O. Turkmen This is me

S. Sensoy This is me

Publication Date September 1, 2013
Published in Issue Year 2013 Volume: 23 Issue: 3

Cite

APA Erdınc, C., Ekıncıalp, A., Yıldız, M., Kabay, T., et al. (2013). Molecular Genetic Diversity in Lake Van Basin Melons (Cucumis melo L.) Based on RAPD and ISSR Markers. Yuzuncu Yıl University Journal of Agricultural Sciences, 23(3), 264-270.
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