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Hakkari İli Bal Arısı (Apis Mellifera L.) Kolonilerindeki Acute Bee Paralysis Virus (ABPV)’ünün Varlığının Saptanması ve Moleküler Karakterizasyonu

Year 2016, Volume: 26 Issue: 2, 174 - 182, 31.05.2016

Mustafa Rüstemoğlu Hikmet Murat Sipahioğlu

Abstract

Hakkari ilinde 2014 yılı Mayıs ve Ağustos ayları arasında 90 farklı arılıkta yürütülen sürvey çalışmalarında Acute bee paralysis virus (ABPV)’ünün varlığı ve yaygınlığı araştırılmıştır. Viral nükleik asidi hassas ve güvenilir biçimde teşhis edebilmek için ABPV’nin genomunda yer alan kılıf protein genine spesifik olarak dizayn edilen ve yaklaşık 476 bç uzunluğunda DNA fragmenti üreten bir çift primer RT-PCR testlerinde kullanılmıştır. Sürvey gerçekleştirilen toplam 90 arılığın sadece iki tanesinde APBV infeksiyonu tespit edilmiş ve virüsün Hakkari ili bal arısı kolonilerinde yaygın olmadığı belirlenmiştir. Tespit edilen ABPV’nin dünyada bilinen diğer izolat ve ırklar ile olan genetik akrabalığını araştırmak için viral genomunda çoğaltılanfragmentler moleküler klonlama yöntemi ile klonlanmış ve DNA dizilemesi gerçekleştirilmiştir. Dizilemesi gerçekleştirilen izolat dünyanın farklı coğrafik bölgelerinde tespit edilen APBV’lere ait diziler ile karşılaştırılmıştır. YürütülenFilogenetik analizler sonucunda Hakkari ilinde tespit edilen APBV izolatının nükleotid dizisinin, Genbankasına kayıtlı dünyadaki diğer izolatlar ile % 85 ile 97 arasında benzerlik gösterdiği tespit edilmiştir. APBV’nin kısmi kılıf protein geni dizisi Gen Bankasına KP259311 ulaşım numarası ile kaydedilmiştir. 

References

  • Antunez K, D’Alessandro B, Corbella E, Ramallo G, Zunino P (2006) Honey bee viruses in Uruguay. Journal of Invertebrate Pathology. 93: 67–70. DOI: 10.1016/j.jip.2006.05.009.
  • Antunez K, Andio M, Garrido-Bailon E, Botias C, Zunino P, Martinez-Salvador A, Mzrin-Hernandez R, Higes M (2012) Low prevalence of honeybee viruses in Spain during 2006 and 2007. Research in Veterinary Science 93: 1441–1445. DOI: 10.1016/j.rvsc.2012.03.006.
  • Bailey L, Gibbs AJ (1964) Acute infection of bees with paralysis virus. Journal of insect pathology 6: 395–407.
  • Bailey L, Milne RG (1969) The multiplication regions and interaction of acute and chronic bee paralysis viruses in adult honeybees. Journal of General Virology 4: 9–14.
  • Bailey L, Gibbs AJ, Woods RD (1963) Two viruses from adult honey bees (Apis mellifera L.). Virology 21: 390–395.
  • Baker AC, Schroeder DC (2008) Occurrence and genetic analysis of picorna-like viruses infecting worker bees of Apis mellifera L. populations in Devon, South West England. Journal of Invertebrate Pathology 98: 239–242. DOI: 10.1016/j.jip.2008.02.010.
  • Bakonyi T, Grabensteiner E, Kolodziejek J, Rusvai M, Topolska G, Ritter W, Nowotny N (2002) Phylogenetic analysis of acute bee paralysis virus strains. Applied and Environmental Microbiology 68: 6446–6450. DOI: 10.1128/AEM.68.12.6446–6450.2002.
  • Bacandritsos N, Granato A, Budge G, Papanastasiou I, Roinioti E, Caldon M, Gallina A, Mutinelli F, Falcaro C (2010) Sudden deaths and colony population decline in Greek honey bee colonies. Journal of Invertebrate Pathology 105: 335-340. DOI: 10.1016/j.jip.2010.08.004.
  • Bekesi L, Ball BV, Dobos-Kovacs M, Bakonyı T, Rusvaı M (1999) Occurrence of Acute Paralysıs Vırus of The Honey Bee (Apis mellıfera) in a Hungarıan Apıary Infested wıth The Parasıtıc Mıte Varroa Jacobsoni. Acta Veterinaria Hungarica 47(3): 319–324.
  • Benjeddou M, Leat N, Allsopp M, Davison S (2001) Detection of acute bee paralysis virus and black queen cell virus from honeybees by reverse transcriptase PCR. Applied and Environmental Microbiology 67: 2384–2387. DOI: 10.1128/AEM.67.5.2384±2387.2001.
  • Berenyi O, Bakonyi T, Derakhshifar I, Köglberger H, Nowotny N (2006) Occurrence of six honeybee viruses in diseased Austrian apiaries. Applied and Environmental Microbiology 72: 2414–2420. DOI: 10.1128/AEM.72.4.2414–2420.2006.
  • Chen J (2010) Molecular Detection of Cucumber Mosaic Virus and Other RNA Viruses Based on New Techniques. Zhejiang University Press. Hangzhou and Springer-Verlag. Berlin. Heidelberg.
  • Chen Y, Siede R (2007) Honey bee viruses. Advances in Virus Research 70: 33–80.
  • De Miranda JR, Cordoni G, Budge G (2010) The Acute bee paralysis virus–Kashmir bee virus-Israeli acute paralysis virus complex. Journal of Invertebrate Pathology 103: 30–47. DOI: 10.1016/j.jip.2009.06.014.
  • De Miranda JR, Bailey L, Ball BV, Blanchard P, Budge G, Chejanovsky N, Chen YP, Gauthıer L, Genersch E, De Graaf D, Rıbıère M, Ryabov E, De Smet L, Van Der Steen JJM (2013) Standard methods for virus research in Apis mellifera. In: Dietemann V, Ellis JD, Neumann P (eds). Volume II: The COLOSS BEEBOOK, standard methods for Apis mellifera pest and pathogen research: Journal of Apicultural Research 52(4): DOI: 10.3896/IBRA.1.52.4.22.
  • Faucon JP, Vitu C, Russo P, Vignomi M (1992) Diagnosis of acute bee paralysis: application to the epidemiology of honey bee viral diseases in France in 1990. Apidologie 23: 139–146.
  • Foissac X, Svanella-Dumas L, Dulucq MJ (2001) Polyvalent detection of fruit tree tricho, capillo and foveaviruses by nested RT-PCR using degenerated and inosine containing primers (PDO RT-PCR). Acta Horticulturae 550: 37-43. DOI: 10.17660/ActaHortic.2001.550.2
  • Francis RM, Kryger P (2012) Single assay detection of Acute bee paralysis virus, Kashmir bee virus.and Israeli acute paralysis virus. Journal of Apicultural Science 56(1): 137-146. DOI: 10.2478/v10289-012-0014-x.
  • Freiberg M, De Jong D, Message D, Cox-Foster D (2012) First report of sacbrood virus in honey bee (Apis mellifera) colonies in Brazil. Genetics and Molecular Research 11: 3310-3314. DOI: 10.4238/2012.September.12.14.
  • Grabensteiner E, Bakonyi T, Ritter W, Pechhacker H, Nowotny N (2007) Development of a multiplex RT-PCR for the simultaneous detection of three viruses of the honeybee (Apis mellifera L.): Acute bee paralysis virus, Black queen cell virus and Sacbrood virus. Journal of Invertebrate Pathology 94: 222–225. DOI: 10.1016/j.jip.2006.11.006.
  • Govan VA, Leat N, Allsopp M, Davison S (2000) Analysis of the complete genome sequence of acute bee paralysis virus shows that it belongs to the novel group of insect-infecting RNA viruses. Virology 277: 457–463.DOI: 10.1006/viro.2000.0616.
  • Gülmez Y, Bursalı A, Tekin Ş (2009) First molecular detection and characterization of Deformed Wing Virus (DWV) in honeybee (Apis melifera) in Turkey. African Journal of Biotechnology 8: 3698-3702.
  • Gümüşova SO, Albayrak H, Kurt M, Yazıcı Z (2010) Prevalence of three honey bee viruses in Turkey. Veterinarski Arhıv 80(6): 779-785.
  • Haddad N, Brake M, Migdadi H, de Miranda JR (2008) First detection of honey bee viruses in Jordan by RT- PCR. Jordan Journal of Agricultural Sciences 4(3): 242-247.
  • Hung ACF, Shimanuki H, Knox DA (1996) Inapparent infection of acute bee paralysis virus and Kashmir bee virus in the US honey bees. American Bee Journal 136: 874– 876.
  • Morimoto T, Kojima Y, Yoshiyama M, Kimura K, Yang B, Kadowaki T (2012) Molecular Identification of Chronic bee paralysis virus Infection in Apis mellifera Colonies in Japan. Viruses 4: 1093-1103. DOI: 10.3390/v4071093.
  • Morse RA, Flottum K (1997) Honey Bee Pests, Predators and Diseases. 3rd ed., Peacock Press. Ohio. USA.
  • Nielsen SL, Nicolaisen M, Kryger P (2008) Incidence of Acute bee paralysis virus, Black queen cell virus, Chronic bee paralysis virus, Deformed wing virus, Kashmir bee virus and Sacbrood virus in honey bees (Apis mellifera) in Denmark. Apidologie 39: 310–314.DOI: 10.1051/apido:2007021.
  • Nordstrom S, Fries I, Aarhus A, Hansen H, Korpela S 1999. Virus infections in Nordic honey bee colonies with no, low or severe Varroa jacobsoni infestation. Apidologie 30: 475–484.
  • Özkırım A, Schiesser A (2013) Israeli acut paralysis virus (IAPV) in Turkish bees. Journal of Apicultural Research 52: 56-57.
  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning: A laboratory manual. 2nd ed., Cold Sprig Harbor Laboratory Press. New York.
  • Sanpa S, Chantawannakul P (2009) Survey of six bee viruses using RT-PCR in Northern Thailand. Journal of Invertebrate Pathology 100: 116–119. DOI: 10.1016/j.jip.2008.11.010.
  • Schmid-Hempel P. (1995) Parasites and Social Insects. Apidologie 26 (3): 255-271.
  • Siede R, Konig M, Buchler R, Failing K, Thiel H-J (2008). A real-time PCR based survey on acute bee paralysis virus in German bee colonies. Apidologie 39: 650–661. DIO: 10.1051/apido:2008044.
  • Tentcheva D, Gauthier L, Zappulla N, Dainat B, Cousserans F, Colin ME, Bergoin M (2004) Prevalence and seasonal variations of six bee viruses in Apis mellifera L. and Varroa destructor mite populations in France. Applied and Environmental Microbiology 70: 7185–7191. DOI: 10.1128/AEM.70.12.7185–7191.2004.
  • Todd HJ, De Miranda JR, Ball VB (2007) Incidence and molecular characterization of viruses found in dying New Zealand honey bee (Apis mellifera) colonies infested with Varroa destructor. Apidologie 38: 354–367. DOI: 10.1051/apido:2007021
  • TÜİK (2014) Türkiye İstatistik Kurumu (TÜİK)/ Veritabanları/ Hayvancılık İstatislikleri. http://tuikapp.tuik.gov.tr/hayvancilikapp/hayvancilik.zul. Ankara (Turkey). (Access date: 30.12.2013).
  • Weinstein-Teixeira E, Chen YP, Message D, Pettis J, Evans JD (2008) Virus infections in Brazilian honey bees. Journal of Invertebrate Pathology 99: 117–119. DOI: 10.1016/j.jip.2008.03.014.

Occurrence and Molecular Characterization of Acute Bee Paralysis Virus (ABPV) in Honeybee (Apis mellifera) Colonies in Hakkari Province

Year 2016, Volume: 26 Issue: 2, 174 - 182, 31.05.2016

Mustafa Rüstemoğlu Hikmet Murat Sipahioğlu

Abstract

A study was carried out during May-August 2014 to investigate the occurrence and prevalence of Acute bee paralysis virus (ABPV) in 90 different apiaries located in Hakkari province (Turkey). To detect the viral nucleic acids, an RT-PCR assay has been implemented for a sensitive and specific diagnosis of ABPV, by using a pair of primer specifically designed to coat protein gene of ABPV, amplifying a 476 bp nucleotide fragment. ABPV was found to occur in 2 samples from two distinct locations among 90 apiaries indicating that ABPV is not widespread in Hakkari province. To understand the molecular relationship between the isolates and the strains of the same virus, the amplified ABPV fragments cloned and sequenced and the sequence was compared with the fragments of ABPV sequences from different geographic origin of the world. The phylogenetic analysis revealed nucleotide identity rates between 85 and 97 %. The partial CP gene of ABPV sequence deposited in the GenBank database with the accession number of KP259311.

References

  • Antunez K, D’Alessandro B, Corbella E, Ramallo G, Zunino P (2006) Honey bee viruses in Uruguay. Journal of Invertebrate Pathology. 93: 67–70. DOI: 10.1016/j.jip.2006.05.009.
  • Antunez K, Andio M, Garrido-Bailon E, Botias C, Zunino P, Martinez-Salvador A, Mzrin-Hernandez R, Higes M (2012) Low prevalence of honeybee viruses in Spain during 2006 and 2007. Research in Veterinary Science 93: 1441–1445. DOI: 10.1016/j.rvsc.2012.03.006.
  • Bailey L, Gibbs AJ (1964) Acute infection of bees with paralysis virus. Journal of insect pathology 6: 395–407.
  • Bailey L, Milne RG (1969) The multiplication regions and interaction of acute and chronic bee paralysis viruses in adult honeybees. Journal of General Virology 4: 9–14.
  • Bailey L, Gibbs AJ, Woods RD (1963) Two viruses from adult honey bees (Apis mellifera L.). Virology 21: 390–395.
  • Baker AC, Schroeder DC (2008) Occurrence and genetic analysis of picorna-like viruses infecting worker bees of Apis mellifera L. populations in Devon, South West England. Journal of Invertebrate Pathology 98: 239–242. DOI: 10.1016/j.jip.2008.02.010.
  • Bakonyi T, Grabensteiner E, Kolodziejek J, Rusvai M, Topolska G, Ritter W, Nowotny N (2002) Phylogenetic analysis of acute bee paralysis virus strains. Applied and Environmental Microbiology 68: 6446–6450. DOI: 10.1128/AEM.68.12.6446–6450.2002.
  • Bacandritsos N, Granato A, Budge G, Papanastasiou I, Roinioti E, Caldon M, Gallina A, Mutinelli F, Falcaro C (2010) Sudden deaths and colony population decline in Greek honey bee colonies. Journal of Invertebrate Pathology 105: 335-340. DOI: 10.1016/j.jip.2010.08.004.
  • Bekesi L, Ball BV, Dobos-Kovacs M, Bakonyı T, Rusvaı M (1999) Occurrence of Acute Paralysıs Vırus of The Honey Bee (Apis mellıfera) in a Hungarıan Apıary Infested wıth The Parasıtıc Mıte Varroa Jacobsoni. Acta Veterinaria Hungarica 47(3): 319–324.
  • Benjeddou M, Leat N, Allsopp M, Davison S (2001) Detection of acute bee paralysis virus and black queen cell virus from honeybees by reverse transcriptase PCR. Applied and Environmental Microbiology 67: 2384–2387. DOI: 10.1128/AEM.67.5.2384±2387.2001.
  • Berenyi O, Bakonyi T, Derakhshifar I, Köglberger H, Nowotny N (2006) Occurrence of six honeybee viruses in diseased Austrian apiaries. Applied and Environmental Microbiology 72: 2414–2420. DOI: 10.1128/AEM.72.4.2414–2420.2006.
  • Chen J (2010) Molecular Detection of Cucumber Mosaic Virus and Other RNA Viruses Based on New Techniques. Zhejiang University Press. Hangzhou and Springer-Verlag. Berlin. Heidelberg.
  • Chen Y, Siede R (2007) Honey bee viruses. Advances in Virus Research 70: 33–80.
  • De Miranda JR, Cordoni G, Budge G (2010) The Acute bee paralysis virus–Kashmir bee virus-Israeli acute paralysis virus complex. Journal of Invertebrate Pathology 103: 30–47. DOI: 10.1016/j.jip.2009.06.014.
  • De Miranda JR, Bailey L, Ball BV, Blanchard P, Budge G, Chejanovsky N, Chen YP, Gauthıer L, Genersch E, De Graaf D, Rıbıère M, Ryabov E, De Smet L, Van Der Steen JJM (2013) Standard methods for virus research in Apis mellifera. In: Dietemann V, Ellis JD, Neumann P (eds). Volume II: The COLOSS BEEBOOK, standard methods for Apis mellifera pest and pathogen research: Journal of Apicultural Research 52(4): DOI: 10.3896/IBRA.1.52.4.22.
  • Faucon JP, Vitu C, Russo P, Vignomi M (1992) Diagnosis of acute bee paralysis: application to the epidemiology of honey bee viral diseases in France in 1990. Apidologie 23: 139–146.
  • Foissac X, Svanella-Dumas L, Dulucq MJ (2001) Polyvalent detection of fruit tree tricho, capillo and foveaviruses by nested RT-PCR using degenerated and inosine containing primers (PDO RT-PCR). Acta Horticulturae 550: 37-43. DOI: 10.17660/ActaHortic.2001.550.2
  • Francis RM, Kryger P (2012) Single assay detection of Acute bee paralysis virus, Kashmir bee virus.and Israeli acute paralysis virus. Journal of Apicultural Science 56(1): 137-146. DOI: 10.2478/v10289-012-0014-x.
  • Freiberg M, De Jong D, Message D, Cox-Foster D (2012) First report of sacbrood virus in honey bee (Apis mellifera) colonies in Brazil. Genetics and Molecular Research 11: 3310-3314. DOI: 10.4238/2012.September.12.14.
  • Grabensteiner E, Bakonyi T, Ritter W, Pechhacker H, Nowotny N (2007) Development of a multiplex RT-PCR for the simultaneous detection of three viruses of the honeybee (Apis mellifera L.): Acute bee paralysis virus, Black queen cell virus and Sacbrood virus. Journal of Invertebrate Pathology 94: 222–225. DOI: 10.1016/j.jip.2006.11.006.
  • Govan VA, Leat N, Allsopp M, Davison S (2000) Analysis of the complete genome sequence of acute bee paralysis virus shows that it belongs to the novel group of insect-infecting RNA viruses. Virology 277: 457–463.DOI: 10.1006/viro.2000.0616.
  • Gülmez Y, Bursalı A, Tekin Ş (2009) First molecular detection and characterization of Deformed Wing Virus (DWV) in honeybee (Apis melifera) in Turkey. African Journal of Biotechnology 8: 3698-3702.
  • Gümüşova SO, Albayrak H, Kurt M, Yazıcı Z (2010) Prevalence of three honey bee viruses in Turkey. Veterinarski Arhıv 80(6): 779-785.
  • Haddad N, Brake M, Migdadi H, de Miranda JR (2008) First detection of honey bee viruses in Jordan by RT- PCR. Jordan Journal of Agricultural Sciences 4(3): 242-247.
  • Hung ACF, Shimanuki H, Knox DA (1996) Inapparent infection of acute bee paralysis virus and Kashmir bee virus in the US honey bees. American Bee Journal 136: 874– 876.
  • Morimoto T, Kojima Y, Yoshiyama M, Kimura K, Yang B, Kadowaki T (2012) Molecular Identification of Chronic bee paralysis virus Infection in Apis mellifera Colonies in Japan. Viruses 4: 1093-1103. DOI: 10.3390/v4071093.
  • Morse RA, Flottum K (1997) Honey Bee Pests, Predators and Diseases. 3rd ed., Peacock Press. Ohio. USA.
  • Nielsen SL, Nicolaisen M, Kryger P (2008) Incidence of Acute bee paralysis virus, Black queen cell virus, Chronic bee paralysis virus, Deformed wing virus, Kashmir bee virus and Sacbrood virus in honey bees (Apis mellifera) in Denmark. Apidologie 39: 310–314.DOI: 10.1051/apido:2007021.
  • Nordstrom S, Fries I, Aarhus A, Hansen H, Korpela S 1999. Virus infections in Nordic honey bee colonies with no, low or severe Varroa jacobsoni infestation. Apidologie 30: 475–484.
  • Özkırım A, Schiesser A (2013) Israeli acut paralysis virus (IAPV) in Turkish bees. Journal of Apicultural Research 52: 56-57.
  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning: A laboratory manual. 2nd ed., Cold Sprig Harbor Laboratory Press. New York.
  • Sanpa S, Chantawannakul P (2009) Survey of six bee viruses using RT-PCR in Northern Thailand. Journal of Invertebrate Pathology 100: 116–119. DOI: 10.1016/j.jip.2008.11.010.
  • Schmid-Hempel P. (1995) Parasites and Social Insects. Apidologie 26 (3): 255-271.
  • Siede R, Konig M, Buchler R, Failing K, Thiel H-J (2008). A real-time PCR based survey on acute bee paralysis virus in German bee colonies. Apidologie 39: 650–661. DIO: 10.1051/apido:2008044.
  • Tentcheva D, Gauthier L, Zappulla N, Dainat B, Cousserans F, Colin ME, Bergoin M (2004) Prevalence and seasonal variations of six bee viruses in Apis mellifera L. and Varroa destructor mite populations in France. Applied and Environmental Microbiology 70: 7185–7191. DOI: 10.1128/AEM.70.12.7185–7191.2004.
  • Todd HJ, De Miranda JR, Ball VB (2007) Incidence and molecular characterization of viruses found in dying New Zealand honey bee (Apis mellifera) colonies infested with Varroa destructor. Apidologie 38: 354–367. DOI: 10.1051/apido:2007021
  • TÜİK (2014) Türkiye İstatistik Kurumu (TÜİK)/ Veritabanları/ Hayvancılık İstatislikleri. http://tuikapp.tuik.gov.tr/hayvancilikapp/hayvancilik.zul. Ankara (Turkey). (Access date: 30.12.2013).
  • Weinstein-Teixeira E, Chen YP, Message D, Pettis J, Evans JD (2008) Virus infections in Brazilian honey bees. Journal of Invertebrate Pathology 99: 117–119. DOI: 10.1016/j.jip.2008.03.014.
There are 38 citations in total.

Details

Journal Section Articles
Authors

Mustafa Rüstemoğlu

Hikmet Murat Sipahioğlu

Publication Date May 31, 2016
Published in Issue Year 2016 Volume: 26 Issue: 2

Cite

APA Rüstemoğlu, M., & Sipahioğlu, H. M. (2016). Occurrence and Molecular Characterization of Acute Bee Paralysis Virus (ABPV) in Honeybee (Apis mellifera) Colonies in Hakkari Province. Yuzuncu Yıl University Journal of Agricultural Sciences, 26(2), 174-182.
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