Abstract
This
study attempts to analyze the karyotype and chromosome structure in five
populations of Quercus libani growing
in Northern Zagros. Pre-treatment, fixation, hydrolysis and staining were
conducted using root meristem and then microscopic samples were prepared and
studied. The results showed that in all studied cells of each population, the
basic chromosome number was x=12 and all of them were diploid. Karyotype
analysis of each population was conducted separately and several indices (TL:
Total Length, LA: Long Arm, SA: Short Arm, CI: Centromer Index, AR: Arm Ratio,
R- value, DRL%: Difference of Relative
Length and TF%: Total Form) were determined. Karyotype formula was 12m in all
studied populations. The length of chromosomes in all populations was estimated
as 0.64-2.08 micrometers. The longest chromosome was observed in chromosome
number 1 from population 1 and the shortest one was related to the chromosome
number 12 from population 5. Considering of chromosomal classification, all the
studied populations were placed in class B of Stebbins which showed that there is
an average symmetry in the studied karyotypes. The other estimated indices also
showed that in chromosomes are relatively symmetric in all populations that indicates
this species is primitive and undeveloped.
Keywords
References
- 1- Agayev, Y.M., 1996, Advanced squash methods for investigation of plant chromosomes. Keynote papers. Fourth Iranian Congress in Crop Production and Breeding Sciences (Aug. 25-28). Esfahan University of Technology, Esfahan, Iran.
- 2- Aykut, Y., Uslu, E. and TekinBabac, 2011. Cytogenetic studies on Quercus L. (Fagaceae) species belonging to Ilex and Cerris section in Turkey, Caryologia , 64(3): 297-301.
- 3- Browicz, k., 1994. Chronology of trees and shrubs in south –west Asia and adjacent Regions. Polish Scientific Publishers, Warsaw, Vol.1: 33-35 & 121.
- 4- Butorina, A.K., and Mozgalina, I.G., 2004. Specific cytogenetic characteristics of Pinus cretaceae and Pinus sylvestris. Russian Journal of Ecology, 35: 156-160.
- 5- Demerico, S., Bianco,P. and Schirone, B., 1995, Karyotype analysis in Quercus spp. Silvae Genetica, 44:66-70.
- 6- Dzialuk, A., Chybichi, I., Welc, M., Sliwinska, E. and Burczyk, J., 2007. Presence of triploids among oak species. Annals of Botany, 99: 956-964.
- 7- Johnson, P.S., Shifley, S.R. and Rogers, R., 2002. The Ecology and Silviculture of oaks. CABI publishing, 503 pp.
- 8- Wright, W.J., 1976. Introduction to forest genetics. Academic press, INC, New York, USA, 463 pp.
Abstract
Bu çalışmanın amacı Kuzey Zagros'ta yetişen beş Quercus libani popülasyonunda karyotip
ve kromozom yapısını analiz etmektir. Ön muamele, fiksasyon, hidroliz ve
boyama, kök meristem kullanılarak yapılmıştır ve daha sonra mikroskobik
numuneler hazırlanıp, incelenmiştir. Sonuçlar, her popülasyonun çalışılan tüm
hücrelerinde, temel kromozom sayısının x = 12 olduğunu ve hepsinin diploid
olduğunu göstermiştir. Her popülasyonun karyotip analizi ayrı ayrı gerçekleştirilmiş
ve birkaç indeks (TL: Toplam Uzunluk, LA: Uzun Kol, SA: Kısa Kol, SI: Sentromer
İndeksi, KO: Kol Oranı, R-değeri, NUF%: Nispi Uzunluk Farklılığı ve TF %:
Toplam Form) belirlenmiştir. Karyotip formülü çalışılan tüm popülasyonlarda 12m
olarak belirlenmiştir. Tüm popülasyonlardaki kromozomların uzunluğu, 0.64-2.08
mikrometre olarak hesaplanmıştır. En uzun kromozom, popülasyon 1'den 1 numaralı
kromozomda ve en kısa kromozom, popülasyon 1'den kromozom 12’de gözlenmiştir.
Kromozomal sınıflandırma göz önüne alındığında, çalışılan tüm popülasyonlar, incelenen
karyotiplerde bir ortalama simetri olduğunu gösteren Stebbins sınıf B'ye
yerleştirilmiştir. Tahmin edilen diğer indeksler, ayrıca tüm popülasyonlarda kromozomların
nispeten simetrik olduğunu; bu türün ilkel ve gelişmemiş olduğunu göstermiştir.
Keywords
References
- 1- Agayev, Y.M., 1996, Advanced squash methods for investigation of plant chromosomes. Keynote papers. Fourth Iranian Congress in Crop Production and Breeding Sciences (Aug. 25-28). Esfahan University of Technology, Esfahan, Iran.
- 2- Aykut, Y., Uslu, E. and TekinBabac, 2011. Cytogenetic studies on Quercus L. (Fagaceae) species belonging to Ilex and Cerris section in Turkey, Caryologia , 64(3): 297-301.
- 3- Browicz, k., 1994. Chronology of trees and shrubs in south –west Asia and adjacent Regions. Polish Scientific Publishers, Warsaw, Vol.1: 33-35 & 121.
- 4- Butorina, A.K., and Mozgalina, I.G., 2004. Specific cytogenetic characteristics of Pinus cretaceae and Pinus sylvestris. Russian Journal of Ecology, 35: 156-160.
- 5- Demerico, S., Bianco,P. and Schirone, B., 1995, Karyotype analysis in Quercus spp. Silvae Genetica, 44:66-70.
- 6- Dzialuk, A., Chybichi, I., Welc, M., Sliwinska, E. and Burczyk, J., 2007. Presence of triploids among oak species. Annals of Botany, 99: 956-964.
- 7- Johnson, P.S., Shifley, S.R. and Rogers, R., 2002. The Ecology and Silviculture of oaks. CABI publishing, 503 pp.
- 8- Wright, W.J., 1976. Introduction to forest genetics. Academic press, INC, New York, USA, 463 pp.
Details
| Primary Language | English |
|---|---|
| Journal Section | Articles |
| Authors | |
| Publication Date | March 29, 2019 |
| Acceptance Date | January 8, 2019 |
| Published in Issue | Year 2019 Volume: 29 Issue: 1 |
