Research Article
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Assessment of Genetic Diversity in Safflower (Carthamus tinctorius L.) Using RAPD Markers

Year 2019, Volume: 29 Issue: 2, 300 - 308, 28.06.2019
https://doi.org/10.29133/yyutbd.560936

Abstract



This research was carried out to determine the
genetic distances and variability between some safflower landraces and
registered varieties. As plant material, four safflower landraces (TR 49119, TR
42630, TR 42670 and TR 64702) and three registered varieties (Yenice 5-38,
Remzibey 05 and Dinçer 5-118) obtained from the Aegean Agricultural Research
Institute, Turkey were used. The safflower varieties were analyzed at the
molecular level using RAPD markers. The polymorphic band ratios of ten RAPD
primers varied from 33.3% to (OPK-11) 80% (OPS-04). The average polymorphic band
ratio was found to be 63.9%. The polymorphism information content values of the
RAPD primers ranged from 0.24 for OPC-03 to 0.46 for OPA-19. The mean PIC value
was determined as 0.38. The mean resolution power value was found to be 3.37,
the effective multiplex ratio value was 4.14, and the marker index value was
1.57. The genetic distances were obtained using NTSYS-pc 2.20j statistic
package program according to Jaccard’s similarity coefficient. The genetic
similarity values of the safflower genotypes varied between 0.61 and 0.85. The
average similarity was calculated as 0.69. The cluster analyses of the RAPD
markers grouped the genotypes into two major clusters (UPGMA dendrogram). With
slight differences, the landraces and registered varieties were included in
separate groups. The TR 64702 line and Remzibey 05 registered variety were
genetically most similar genotypes with a value of 0.85.

Supporting Institution

The Commission of Scientific Research Projects of Ege University

Project Number

2011/ZRF/073

Thanks

The author thanks the Commission of Scientific Research Projects of Ege University for the financial support to carry out this study (Project no: 2011/ZRF/073).

References

  • Amini, F., Saeidi, G., Arzani, A. (2008). Study of genetic diversity in safflower genotypes using agro-morphological traits and RAPD markers. Euphytica, 163:21–30.
  • Babaoğlu, M. (2007). Aspir ve tarımı. Trakya Tarımsal Araştırmalar Enstitüsü, Edirne.
  • Baydar, H., Kara, N. (2010). Aspir (Carthamus tinctorius L.)’in Büyüme ve Gelisme Dönemlerinde Vejetatif ve Genaratif Organlarda Kuru Madde Birikimi, Süleyman Demirel Üniversitesi, Fen Bilimleri Enstitüsü Dergisi, 14-2( 2010),148-155.
  • Er, C., Geçit, H.H., Başalma, D., Avcı, M., Kaya, D., Akdoğan, G., Çevik, N., Güneş, E., Ada, R., Koç, H., Koşar, F., Şentürk, Ş. (2010). Orta Anadolu Şartlarında Yağ Bitkilerinin Üretim Deseni İçerisinde Yeralabilme Potansiyeli Sonuç Raporu. TÜBİTAK Proje No:105O592.
  • FAOSTAT. (2017). FAO Statistical Databases. http://www.fao.org/faostat/en/ Accessed 06.04.2019
  • Jaccard, P. (1908) Novelles recherches sur la distribution florale. Bulletin de la Société vaudoise des sciences naturelles, 44: 223-270.
  • Mahasi, M. J., Wachira F. N., Pathak R. S. and Riungu T. C. (2009). Genetic polymorphism in exotic safflower (Carthamus tinctorious L.) using RAPD markers, Journal of Plant Breeding and Crop Science Vol. 1(1). pp. 008-012.
  • Mantel, N. A. (1967) The detection of disease clustering and a generalized regression approach. Cancer Research, 27: 209-220.
  • Milbourne, D., Meyer, R., Bradshaw, J.E., Baird, E., Bonar, N., Provan, J., Powell, W. and Waugh, R. (1997) Comparison of PCR-Based Marker Systems for the Analysis of Genetic Relationships in Cultivated Potato. Mol. Breed. 3(2): 127-136.
  • Mullis, K. B., F. Faloona, S. Scharf, R. Saiki, G. Horn, H. Erlich. (1986) Specific Enzymatic Amplification of DNA In Vitro: The Polymerase Chain Reaction. Cold Spring Harb Symp Quant Biol 51: 263-273. https://doi.org/10.1101/SQB.1986.051.01.032.
  • Powell, W., M. Morgante, C. Andre, M. Hanafey, J. Vogel, S. Tingey and Rafalski, A. (1996) The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Molecular Breeding, 2: 225-238.
  • Prevost, A. and Wilkinson, M. J. (1999) A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars. Theoretical and Applied Genetics, 98: 107-112.
  • Rohlf, FJ. (2000). NTSYS-PC, Numerical taxonomy and multi-variate analysis system, version 2.20j. Exeter Publications, Setauket, New York
  • Safavi, S.A., Pourdad, S.S., Taeb, M., and Khosroshahli, M. (2010). Assessment of genetic variation among safflower (Carthamus tinctorius L.) accessions using agro-morphological traits and molecular markers, Journal of Food, Agriculture & Environment Vol.8 (3&4): 616 - 625.
  • Sehgal, D. and Raina, S.N. (2005) Genotyping safflower (Carthamus tinctorius) cultivars by DNA fingerprints. Euphytica, 146: 67–76.
  • Sehgal, D., Rajpal, V.R., Raina, S.N., Sasanuma, T., Sasakuma, T. (2009) Assaying polymorphism at DNA level for genetic diversity diagnostics of the safflower (Carthamus tinctorius L.) World germplasm resources. Genetica, 135:457–470.
  • Tonguç, M., Baydar, H., Karakurt, Y., Erbas, S. (2010) Aspirde Genetik Çesitliligin Morfolojik ve Moleküler Yöntemlerle Karakterizasyonu ve İleri Hatlarda Genetik Harita Çıkarma imkanlarının Araştırılması Sonuç Raporu. TÜBİTAK Proje No:107O504.
  • TÜİK. (2018). Türkiye İstatistik Kurumu http://www.tuik.gov.tr/Start.do Accessed 06.04.2019
  • Weiss, E.A. (2000) Safflower. In: Oilseed Crops, Blackwell Sci. Ltd., Victoria, Australia, pp 93-129
  • Williams, J. G. K., Kubelik, A. R., Livak, K. J., Rafalski, J. A., Tingey, S. V. (1990) DNA Polymorphisms Amplified by Arbitrary Primers are Useful as Genetic Marker. Nucleic Acid Research, 18, 22, 6531-6535.

Aspir (Carthamus tinctorius L.) 'de Genetik Çeşitliliğin RAPD Markörleri Kullanarak Değerlendirilmesi

Year 2019, Volume: 29 Issue: 2, 300 - 308, 28.06.2019
https://doi.org/10.29133/yyutbd.560936

Abstract

Çalışmada, Ege Tarımsal Araştırma Enstitüsü
Müdürlüğü’nden temin edilmiş olan 4 yerel çeşit (TR 49119, TR 42630, TR 42670
ve TR 64702) ve üç adet tescilli aspir çeşidi (Yenice 5-38, Remzibey 05 ve
Dinçer 5-118) materyal olarak kullanılmıştır. Aspir çeşitleri, RAPD markörleri
kullanılarak moleküler düzeyde analiz edilmiştir. Kullanılan on adet RAPD
primerinin polimorfik bant oranı % 33.3 (OPK-11) ile % 80 (OPS-04) arasında
değişim göstermiştir. Ortalama polimorfik bant oranı % 63.9 olarak
belirlenmiştir. RAPD primerlerinin polimorfik bilgi içeriği (PIC) değerleri
incelendiğinde, 0.24 (OPC-03) ile 0.46 (OPA-19) arasında değişim gösterdiği
gözlenmiştir. Ortalama PIC değeri 0.38 olarak bulunmuştur. Ortalama çözümleme
gücü değeri 3.37, etkili multipleks oran değeri 4.14 ve markör endeks değeri
ise 1.57 olarak belirlenmiştir. NTSYS-pc 2.20j istatistik paket programında
Jaccard’ın benzerlik katsayısına göre genotipler arasındaki genetik uzaklık
değerleri elde edilmiştir. Aspir genotiplerine ait genetik benzerlik değerleri
0.61 ile 0.85 arasında değişim göstermiştir. Ortalama benzerlik oranı 0.69
olarak 
bulunmuştur. Aspir çeşitleri UPGMA gruplandırmasına
göre iki gruba ayrılmışlardır. Küçük bir nüansla yerli ve tescilli çeşitler
ayrı gruplarda yer almıştır. TR 64702 yerel çeşidi ile Remzibey 05 tescilli
çeşidi 0.85 değeriyle genetik olarak en benzer genotipler olarak gözlenmiştir.

Project Number

2011/ZRF/073

References

  • Amini, F., Saeidi, G., Arzani, A. (2008). Study of genetic diversity in safflower genotypes using agro-morphological traits and RAPD markers. Euphytica, 163:21–30.
  • Babaoğlu, M. (2007). Aspir ve tarımı. Trakya Tarımsal Araştırmalar Enstitüsü, Edirne.
  • Baydar, H., Kara, N. (2010). Aspir (Carthamus tinctorius L.)’in Büyüme ve Gelisme Dönemlerinde Vejetatif ve Genaratif Organlarda Kuru Madde Birikimi, Süleyman Demirel Üniversitesi, Fen Bilimleri Enstitüsü Dergisi, 14-2( 2010),148-155.
  • Er, C., Geçit, H.H., Başalma, D., Avcı, M., Kaya, D., Akdoğan, G., Çevik, N., Güneş, E., Ada, R., Koç, H., Koşar, F., Şentürk, Ş. (2010). Orta Anadolu Şartlarında Yağ Bitkilerinin Üretim Deseni İçerisinde Yeralabilme Potansiyeli Sonuç Raporu. TÜBİTAK Proje No:105O592.
  • FAOSTAT. (2017). FAO Statistical Databases. http://www.fao.org/faostat/en/ Accessed 06.04.2019
  • Jaccard, P. (1908) Novelles recherches sur la distribution florale. Bulletin de la Société vaudoise des sciences naturelles, 44: 223-270.
  • Mahasi, M. J., Wachira F. N., Pathak R. S. and Riungu T. C. (2009). Genetic polymorphism in exotic safflower (Carthamus tinctorious L.) using RAPD markers, Journal of Plant Breeding and Crop Science Vol. 1(1). pp. 008-012.
  • Mantel, N. A. (1967) The detection of disease clustering and a generalized regression approach. Cancer Research, 27: 209-220.
  • Milbourne, D., Meyer, R., Bradshaw, J.E., Baird, E., Bonar, N., Provan, J., Powell, W. and Waugh, R. (1997) Comparison of PCR-Based Marker Systems for the Analysis of Genetic Relationships in Cultivated Potato. Mol. Breed. 3(2): 127-136.
  • Mullis, K. B., F. Faloona, S. Scharf, R. Saiki, G. Horn, H. Erlich. (1986) Specific Enzymatic Amplification of DNA In Vitro: The Polymerase Chain Reaction. Cold Spring Harb Symp Quant Biol 51: 263-273. https://doi.org/10.1101/SQB.1986.051.01.032.
  • Powell, W., M. Morgante, C. Andre, M. Hanafey, J. Vogel, S. Tingey and Rafalski, A. (1996) The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Molecular Breeding, 2: 225-238.
  • Prevost, A. and Wilkinson, M. J. (1999) A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars. Theoretical and Applied Genetics, 98: 107-112.
  • Rohlf, FJ. (2000). NTSYS-PC, Numerical taxonomy and multi-variate analysis system, version 2.20j. Exeter Publications, Setauket, New York
  • Safavi, S.A., Pourdad, S.S., Taeb, M., and Khosroshahli, M. (2010). Assessment of genetic variation among safflower (Carthamus tinctorius L.) accessions using agro-morphological traits and molecular markers, Journal of Food, Agriculture & Environment Vol.8 (3&4): 616 - 625.
  • Sehgal, D. and Raina, S.N. (2005) Genotyping safflower (Carthamus tinctorius) cultivars by DNA fingerprints. Euphytica, 146: 67–76.
  • Sehgal, D., Rajpal, V.R., Raina, S.N., Sasanuma, T., Sasakuma, T. (2009) Assaying polymorphism at DNA level for genetic diversity diagnostics of the safflower (Carthamus tinctorius L.) World germplasm resources. Genetica, 135:457–470.
  • Tonguç, M., Baydar, H., Karakurt, Y., Erbas, S. (2010) Aspirde Genetik Çesitliligin Morfolojik ve Moleküler Yöntemlerle Karakterizasyonu ve İleri Hatlarda Genetik Harita Çıkarma imkanlarının Araştırılması Sonuç Raporu. TÜBİTAK Proje No:107O504.
  • TÜİK. (2018). Türkiye İstatistik Kurumu http://www.tuik.gov.tr/Start.do Accessed 06.04.2019
  • Weiss, E.A. (2000) Safflower. In: Oilseed Crops, Blackwell Sci. Ltd., Victoria, Australia, pp 93-129
  • Williams, J. G. K., Kubelik, A. R., Livak, K. J., Rafalski, J. A., Tingey, S. V. (1990) DNA Polymorphisms Amplified by Arbitrary Primers are Useful as Genetic Marker. Nucleic Acid Research, 18, 22, 6531-6535.
There are 20 citations in total.

Details

Primary Language English
Subjects Botany
Journal Section Articles
Authors

Refiye Refika Akçalı Gıachıno 0000-0002-6473-7250

Duygu İnan This is me 0000-0001-6874-6755

Project Number 2011/ZRF/073
Publication Date June 28, 2019
Acceptance Date June 13, 2019
Published in Issue Year 2019 Volume: 29 Issue: 2

Cite

APA Akçalı Gıachıno, R. R., & İnan, D. (2019). Assessment of Genetic Diversity in Safflower (Carthamus tinctorius L.) Using RAPD Markers. Yuzuncu Yıl University Journal of Agricultural Sciences, 29(2), 300-308. https://doi.org/10.29133/yyutbd.560936
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Yuzuncu Yil University Journal of Agricultural Sciences by Van Yuzuncu Yil University Faculty of Agriculture is licensed under a Creative Commons Attribution 4.0 International License.