Yaprağı Yenen Sebzelerdeki Mikroorganizma Türlerinin MALDI-TOF MS (Matris Destekli Lazer Desorpsiyon/İyonizasyon Uçuş Süresi Kütle Spektrometresi) Tekniği Kullanılarak Tanılanması

Year 2019, Volume: 29 Issue: 4, 595 - 603, 31.12.2019

Aysun Uysul Şener Kurt Soner Soylu , Emine Mine Soylu Merve Kara

https://doi.org/10.29133/yyutbd.627850

Cited By: 13

Abstract

Gıda
kaynaklı fungal ve bakteriyel patojenler giderek taze meyve ve sebzelerle
ilişkilendirilmektedir. Taze üretilen bitkilerin bakteriyel kolonizasyonu, dış
dokuda yüksek seviyelerde oluşabilir, ancak bitki dokusu içerisinde bakteriler
de tespit edilmektedir. Hatay ilinin Defne ve Antakya ilçelerinde farklı sebze
pazarlarından marul, ıspanak, lahana, roka, nane, maydanoz, dereotu, pırasa
gibi yapraklı sebzeler toplanmıştır. Mikrobiyolojik analizler için patates
dekstroz agar, nutrient agar ve kanlı agar besi yerleri kullanılmıştır. Bu
mikroorganizmaların tanılanması, fenotipik karakterler ve matris destekli lazer
desorpsiyon/iyonizasyon uçuş süresi kütle spektrometresi (MALDI-TOF MS)
tekniğine dayalı olarak gerçekleştirilmiştir. MALDI-TOF MS analizi sonucuna
göre mikroorganizmaların dağılımı incelendiğinde, tür düzeyinde 60 adet ile en
fazla bakteri türleri tanılanırken, bunu 4 adet fungus ve 1 adet maya
izlemiştir. En fazla mikroorganizma türü marul örneklerinden elde edilmiştir. Cins
düzeyinde dağılımları incelendiğinde; 9 tür ile Pseudomonas başta gelirken, bunu 5 tür ile Lactobacillus, 4 tür ile Acinetobacter
ve 2 tür ile Klebsiella, Citrobacter,
Enterobacter, Lysinibacillus, Pantoea 1 tür Bacillus, 1 tür Escherichia
coli, 1 tür Exiguobacterium
cinsleri izlemiştir. Mikroorganizma türlerinin koloni yoğunlukları
incelendiğinde, en yoğun koloni Pseudomonas
ve Acinetobacter cinsine ait türlerde
belirlenmiştir. En az koloni yoğunluğu funguslarda gözlenmiştir. Çalışma
sonucunda Salmonella sp, Klebsiella pneumoniae, Escherichia coli, Bacillus cereus, Klebsiella oxytoca türleri insan sağlığı için en
tehlikeli türler olarak bulunmuştur.

Keywords

MALDI-TOF MS, Mikroorganizma, Taze sebze, Tanı

Supporting Institution

Hatay Mustafa Kemal Üniversitesi Bilimsel Araştırma Projeleri Komisyon Başkanlığı

Project Number

18.M.026

Thanks

Bu çalışma, Hatay Mustafa Kemal Üniversitesi Bilimsel Araştırma Projeleri Komisyon Başkanlığına tarafından 18.M.026 nolu proje kapsamında desteklenmiş ve Hatay Mustafa Kemal Üniversitesi Bitki Sağlığı Kliniği Uygulama ve Araştırma Merkezi laboratuvarlarında yürütülmüştür.

Identification of Microorganism Species in Leafy Vegetables Using MALDI-TOF MS (Matrix Assisted Laser Desorption / Ionization Mass Spectrometry) Technique

Abstract

The
foodborne fungal and bacterial pathogens are increasingly associated with fresh
fruit and vegetables. Bacterial colonization of fresh produce plants can occur
to high levels on the external tissue but bacteria have also been detected
within plant tissue. Leafy vegetables such as lettuce, spinach, cabbage,
rocket, mint, parsley, dill, and leek were collected from different vegetable
markets in the counties of Defne and Antakya of Hatay province. Growing media potato
dextrose agar, nutrient agar and blood agar media were used for microbiological
analysis. Their identification were performed based on phenotypic characters
and matrix-assisted laser desorption/ionization time-of-flight mass
spectrometry (MALDI-TOF MS) technique. According to the results of MALDI-TOF MS
analysis, when the distribution of microorganisms were examined, the most
bacterial species were identified with 60 species at the species level,
followed by 4 fungi and 1 yeast.   The
most microorganism species were obtained from lettuce samples.   When the distribution of species level is
examined; firstly, 9 species with Pseudomonas,
5 species with Lactobacillus, 4
species with Acinetobacter and 2
species with Klebsiella, Citrobacter,
Enterobacter, Lysinibacillus were obtained and Pantoea one species Bacillus,
one species Escherichia coli, one
species followed by Exiguobacterium genera.
When the colony density of microorganism species were examined, the most
intensive colony was determined in the species belonging to the genus Pseudomonas and Acinetobacter. At least colony density was observed in fungi. In
result, Salmonella sp, Klebsiella pneumoniae, Escherichia coli,
Bacillus cereus, Klebsiella oxytoca species were found to be very dangerous
species for human health.

Keywords

MALDI-TOF MS, Microorganism, fresh vegetables, identification

Project Number

18.M.026

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