Research Article
Effect Of 24- Epi Brassinolide on Some BiochemicalCharacteristics of Parus and Gaviota Strawberry Cultivars Under Heat Stress Conditions
Abstract
The study was arranged as factorial experiment in a completely randomized design with three applications of cultivar (Parus and Gaviota), 24- Epi brassinolide concentrations (0, 1 and 2 mgl-1) and temperature (normal and stress) as three replications. Some of the measuring characteristics showed that there was a significant difference among the treatments. Under heat stress conditions, foliar application of 24- Epi brassinolide at 1 mgl-1 concentration increased the amount of catalase and superoxide dismutase activity in leaves of Parus cultivar. Both cultivars fruits showed the highest total soluble solid contents in treatment 24- Epi brassinolide spraying at 2 mgl-1 concentrations under normal temperature. The highest total phenolics was in Gaviota cultivar treated with 24- Epi brassinolide spraying at 2 mgl-1 concentrations under normal temperature. Both cultivars had the lowest flavonoid in treatments without 24- Epi brassinolide and with 24- Epi brassinolide at 2 mgl-1 concentrations under heat stress conditions. In Gaviota cultivar, application of high concentration of 24- Epi brassinolide (2 mgl-1) prevented the reduction of total anthocyanin under heat stress conditions.
Keywords
References
- Adak, N., Gubbuk, H., & Tetik, N. (2018). Yield, quality and biochemical properties of various strawberry cultivars under water stress. Journal Of The Science Of Food And Agriculture , 98, 304-311.Akhtar, A., Abbasi, N. A., & Hussain, A. (2010). Effect of calcium chloride treatments on quality characteristics of loquat fruit during storage. Pakistan Journal Of Botany, 42, 181-188.Aebi, H. (1984). Catalase in vitro. Methods in Enzymology, 105, 121-126.Ayub, R. A., Reis, L., Lopes, P. Z., & Bosetto, L. (2018). Ethylene and brassinosteroid effect on strawberry ripening after field spray. Revista Brasileira de Fruticultura, 40, 1-6.Beauchamp, C., & Fridovich, I. (1971). Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry, 44, 276-287. Champa, W. H., Gill, M., Mahajan, B., Aror, N., & Bedi, S. (2015). Brassinosteroids improve quality of table grapes (Vitis vinifera L.) cv. flame seedless. Tropical Agricultural Research, 26, 26-31. Change, B., & Maehly, A. (1955). Assay of catalases and peroxidase. Methods Enzymol, 2, 764-775. Dela, G., Or, E., Ovadia, R., Nissim-Levi, A., Weiss, D., & Oren-Shamir, M. (2003). Changes in anthocyanin concentration and composition in ‘Jaguar’rose flowers due to transient high-temperature conditions. Plant Science, 164, 333-340. Gao, S., Ouyang, C., Wang, S., Xu, Y., Tang, L., & Chen, F. (2008). Effects of salt stress on growth, antioxidant enzyme and phenylalanine ammonia-lyase activities in Jatropha curcas L. seedlings. Plant Soil Environ, 54, 374-381. Gomes M, Torres A, Campostrini E, Bressan-Smith R, Zullo M, Ferraz M & Nunez M (2013). Brassinosteroid analogue affects the senescence in two papaya genotypes submitted to drought stress. Theoretical and Experimental Plant Physiology, 25, 186-195. Gunes, A., Pilbeam, D. J., Inal, A., & Coban, S. (2008). Influence of silicon on sunflower cultivars under drought stress, I: Growth, antioxidant mechanisms, and lipid peroxidation. Communications in Soil Science and Plant Analysis, 39, 1885-1903. Gruszka, D. (2013). The brassinosteroid signaling pathway new key players and interconnections with other signaling networks crucial for plant development and stress tolerance. Molecular Science, 14, 8740-8774. Hayat, S., & Ahmad, A. (2010). Brassinosteroids: a class of plant hormone. Springer Science and Business Media, 18-25.Johnson, M., & Lingakumar, K. (2011). Effect of crude brassinosteroid extract on growth and biochemical changes of Gosssypium hirsutum L. and Vigna mungo L. Stress Physiology and Biochemistry, 7, 324-334. Kaplan, U., & Gokbayrak, Z. (2012). Effect of 22 (S), 23 (S)-Homobrassinolide on adventitious root formation in grape rootstocks. South African Journal for Enology and Viticulture, 33, 253. Klunklin, W., & Savage, G. (2017). Effect on quality characteristics of tomatoes grown under well-watered and drought stress conditions. Foods: 6(8), 56. Kumar, S., Sirhindi, G., Bhardwaj, R., Kumar, M., & Arora, P. (2012). Role of 24-Epibrassinolide in amelioration of high temperature stress through antioxidant defense system in Brassica juncea L. Plant Stress, 6, 55-58. Laxmi, A., Paul, L. K., Peters, J. L., & Khurana, J. P. (2004). Arabidopsis constitutive photomorphogenic mutant, bls1, displays altered brassinosteroid response and sugar sensitivity. Plant Molecular Biology, 56, 185-201Lotfi, H., Barzegar, T., & Ghahremani Z. (2015). Assessment of growth, yield and fruit quality of two iranian cantaloupe accessions under different irrigation levels. Sustainagriculture and Production Science, 26, 107-116.Maecka, A., Jarmuszkiewicz, W. A., & Tomaszewska, B. (2001). Antioxidative defense to lead stress in subcellular compartments of pea root cells. Acta Bioquimica Polonica, 48, 687-698.Mazorra, L. M., Nunez, M., Hechavarria, F. C., & Sanchez-Blanco, M. J. (2002). Influence of brassinosteroids on antioxidant enzymes activity in tomato under different temperatures. Biologia Plantarum, 45, 593-596.Mirzaee, M., Moieni, A., & Ghanati, F. (2013). Effects of drought stress on the lipid peroxidation and antioxidant enzyme activities in two canola (Brassica napus L.) cultivars. Journal of Agricultural Science and Technology, 15, 593-602.Niu, J.-H., Ahmad Anjum, S., Wang, R., Li, J.-H., Liu, M.-R., Song, J.-X., & Zong, X.-F. (2016). Exogenous application of brassinolide can alter morphological and physiological traits of Leymus chinensis (Trin.) Tzvelev under room and high temperatures. Agricultural Research, 76, 27-33. Ramani, M. M. (2015). Effect of shoot thining and 28- homobrassinolid sprsy on growth, flowering, yield and quality of magno cv. Fruit Science Department, Aspee College of Horticulture and Forestry Navsari Agricultural University, 1-140.Reddy, K. R., & Kakani, V. (2007). Screening Capsicum species of different origins for high temperature tolerance by in vitro pollen germination and pollen tube length. Scientia Horticulturae, 112, 130-135. Rodrigues, E., Poerner, N., Rockenbach, I. I., Gonzaga, L. V., Mendes, C. R., & Fett, R. (2011). Phenolic compounds and antioxidant activity of blueberry cultivars grown in Brazil. Food Science and Technology, 31, 911-917. Roghabadi, M. A., & Pakkish, Z. (2014). Role of brassinosteroid on yield, fruit quality and postharvest storage of ‘Tak Danehe Mashhad’sweet cherry (Prunus avium L.). Agricultural Communications, 2, 49-56. Suarez, L., Zarco-Tejada, P. J., Gonzalez-Dugo, V., Berni, J., Sagardoy, R., Morales, F., & Fereres, E. (2010). Detecting water stress effects on fruit quality in orchards with time-series PRI airborne imagery. Remote Sensing of Environment, 114, 286-298.Swamy, K., Vardhini, B., Ramakrishna, B., Anuradha, S., Siddulu, N., & Rao, S. (2014). Role of 28-homobrassinolide on growth biochemical parameters of Trigonella foenugraecum L. plants subjected to lead toxicity. Multidisciplinary and Current Research, 2, 317. Upadhyay, P., & Maier, C. (2016). Effects of 17 β-estradiol on growth, primary metabolism, phenylpropanoid-flavonoid pathways and pathogen resistance in arabidopsis thaliana. Plant Science, 7, 1693-1710.Uprety, D. (2006). Interactive effect of moisture stress and elevated CO2 on the oxidative stress in brassica species. Journal of Food, Agriculture and Environmen, 4, 298-305. Valizadeh, M., Moharamnejad, S., Ahmadi, M., & Mohammadzadeh Jalaly, H. (2013). Changes in activity profile of some antioxidant enzymes in alfalfa half-sib families under salt stress. Journal of Agricultural Science and Technology, 15, 801-809. Xi, Z., Zhang, Z., Huo, S., Luan, L., Gao, X., Ma, L., & Fang, Y. (2013). Regulating the secondary metabolism in grape berry using exogenous 24-epibrassinolide for enhanced phenolics content and antioxidant capacity. Food chemistry, 141, 3056-3065.Yadava, P., Kaushal, J., Gautam, A., Parmar, H., & Singh, I. (2016). Physiological and biochemical effects of 24-epibrassinolide on heat-stress adaptation in maize (Zea mays L.). Natural Sciences, 8, 171. Zhu, F., Yun, Z., Ma, Q., Gong, Q., Zeng, Y., Xu, J., & Deng, X. (2015). Effects of exogenous 24-Epibrassinolide treatment on postharvest quality and resistance of Satsuma mandarin (Citrus unshiu). Postharvest Biology and Technology, 100, 8-15.
24-Epi Brassinolidin, Sıcaklık Stresi Koşullarında Parus ve Gaviota Çilek Çeşitlerinin Bazı Biyokimyasal Özellikleri Üzerine Etkisi
Abstract
Çalışma, çeşit (Parus ve Gaviota), 24-Epi brassinolid konsantrasyonları (0, 1 ve 2 mg/l) ve sıcaklık (normal ve stres) olmak üzere üç uygulama 3 tekerrürlü olarak tesadüf parselleri faktöriyel deneme deseninde düzenlenmiştir. Ölçüm özelliklerinden bazıları, uygulamalar arasında önemli bir fark olduğunu göstermiştir. Sıcaklık stresi koşulları altında, 24 mg-Epi brassinolidin 1 mg/l konsantrasyonunda yapraktan uygulanması, Parus çeşidinin yapraklarındaki katalaz ve süperoksit dismutaz aktivitesini arttırmıştır. Her iki çeşidin meyveleri, normal sıcaklık altında 2 mg/l konsantrasyonunda 24-Epi brassinolid uygulamasında en yüksek suda çözünür kuru madde içeriğine sahip olmuştur. En yüksek toplam fenolikler, normal sıcaklık altında 2 mg/l konsantrasyonunda 24-Epi brassinolid uygulanan Gaviota çeşidinde tespit edilmiştir. Her iki çeşit, 24-Epi brassinolid içermeyen ve 24-Epi brassinolid içeren uygulamalarda, sıcaklık stresi koşulları altında 2 mg/l konsantrasyonunda en düşük flavonoid içeriğine sahip olmuştur. Gaviota çeşidinde, yüksek konsantrasyonda 24-Epi brassinolid (2 mg/l) uygulanması, sıcaklık stresi koşulları altında toplam antosiyanin miktarının azalmasını önlemiştir.
Keywords
References
- Adak, N., Gubbuk, H., & Tetik, N. (2018). Yield, quality and biochemical properties of various strawberry cultivars under water stress. Journal Of The Science Of Food And Agriculture , 98, 304-311.Akhtar, A., Abbasi, N. A., & Hussain, A. (2010). Effect of calcium chloride treatments on quality characteristics of loquat fruit during storage. Pakistan Journal Of Botany, 42, 181-188.Aebi, H. (1984). Catalase in vitro. Methods in Enzymology, 105, 121-126.Ayub, R. A., Reis, L., Lopes, P. Z., & Bosetto, L. (2018). Ethylene and brassinosteroid effect on strawberry ripening after field spray. Revista Brasileira de Fruticultura, 40, 1-6.Beauchamp, C., & Fridovich, I. (1971). Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry, 44, 276-287. Champa, W. H., Gill, M., Mahajan, B., Aror, N., & Bedi, S. (2015). Brassinosteroids improve quality of table grapes (Vitis vinifera L.) cv. flame seedless. Tropical Agricultural Research, 26, 26-31. Change, B., & Maehly, A. (1955). Assay of catalases and peroxidase. Methods Enzymol, 2, 764-775. Dela, G., Or, E., Ovadia, R., Nissim-Levi, A., Weiss, D., & Oren-Shamir, M. (2003). Changes in anthocyanin concentration and composition in ‘Jaguar’rose flowers due to transient high-temperature conditions. Plant Science, 164, 333-340. Gao, S., Ouyang, C., Wang, S., Xu, Y., Tang, L., & Chen, F. (2008). Effects of salt stress on growth, antioxidant enzyme and phenylalanine ammonia-lyase activities in Jatropha curcas L. seedlings. Plant Soil Environ, 54, 374-381. Gomes M, Torres A, Campostrini E, Bressan-Smith R, Zullo M, Ferraz M & Nunez M (2013). Brassinosteroid analogue affects the senescence in two papaya genotypes submitted to drought stress. Theoretical and Experimental Plant Physiology, 25, 186-195. Gunes, A., Pilbeam, D. J., Inal, A., & Coban, S. (2008). Influence of silicon on sunflower cultivars under drought stress, I: Growth, antioxidant mechanisms, and lipid peroxidation. Communications in Soil Science and Plant Analysis, 39, 1885-1903. Gruszka, D. (2013). The brassinosteroid signaling pathway new key players and interconnections with other signaling networks crucial for plant development and stress tolerance. Molecular Science, 14, 8740-8774. Hayat, S., & Ahmad, A. (2010). Brassinosteroids: a class of plant hormone. Springer Science and Business Media, 18-25.Johnson, M., & Lingakumar, K. (2011). Effect of crude brassinosteroid extract on growth and biochemical changes of Gosssypium hirsutum L. and Vigna mungo L. Stress Physiology and Biochemistry, 7, 324-334. Kaplan, U., & Gokbayrak, Z. (2012). Effect of 22 (S), 23 (S)-Homobrassinolide on adventitious root formation in grape rootstocks. South African Journal for Enology and Viticulture, 33, 253. Klunklin, W., & Savage, G. (2017). Effect on quality characteristics of tomatoes grown under well-watered and drought stress conditions. Foods: 6(8), 56. Kumar, S., Sirhindi, G., Bhardwaj, R., Kumar, M., & Arora, P. (2012). Role of 24-Epibrassinolide in amelioration of high temperature stress through antioxidant defense system in Brassica juncea L. Plant Stress, 6, 55-58. Laxmi, A., Paul, L. K., Peters, J. L., & Khurana, J. P. (2004). Arabidopsis constitutive photomorphogenic mutant, bls1, displays altered brassinosteroid response and sugar sensitivity. Plant Molecular Biology, 56, 185-201Lotfi, H., Barzegar, T., & Ghahremani Z. (2015). Assessment of growth, yield and fruit quality of two iranian cantaloupe accessions under different irrigation levels. Sustainagriculture and Production Science, 26, 107-116.Maecka, A., Jarmuszkiewicz, W. A., & Tomaszewska, B. (2001). Antioxidative defense to lead stress in subcellular compartments of pea root cells. Acta Bioquimica Polonica, 48, 687-698.Mazorra, L. M., Nunez, M., Hechavarria, F. C., & Sanchez-Blanco, M. J. (2002). Influence of brassinosteroids on antioxidant enzymes activity in tomato under different temperatures. Biologia Plantarum, 45, 593-596.Mirzaee, M., Moieni, A., & Ghanati, F. (2013). Effects of drought stress on the lipid peroxidation and antioxidant enzyme activities in two canola (Brassica napus L.) cultivars. Journal of Agricultural Science and Technology, 15, 593-602.Niu, J.-H., Ahmad Anjum, S., Wang, R., Li, J.-H., Liu, M.-R., Song, J.-X., & Zong, X.-F. (2016). Exogenous application of brassinolide can alter morphological and physiological traits of Leymus chinensis (Trin.) Tzvelev under room and high temperatures. Agricultural Research, 76, 27-33. Ramani, M. M. (2015). Effect of shoot thining and 28- homobrassinolid sprsy on growth, flowering, yield and quality of magno cv. Fruit Science Department, Aspee College of Horticulture and Forestry Navsari Agricultural University, 1-140.Reddy, K. R., & Kakani, V. (2007). Screening Capsicum species of different origins for high temperature tolerance by in vitro pollen germination and pollen tube length. Scientia Horticulturae, 112, 130-135. Rodrigues, E., Poerner, N., Rockenbach, I. I., Gonzaga, L. V., Mendes, C. R., & Fett, R. (2011). Phenolic compounds and antioxidant activity of blueberry cultivars grown in Brazil. Food Science and Technology, 31, 911-917. Roghabadi, M. A., & Pakkish, Z. (2014). Role of brassinosteroid on yield, fruit quality and postharvest storage of ‘Tak Danehe Mashhad’sweet cherry (Prunus avium L.). Agricultural Communications, 2, 49-56. Suarez, L., Zarco-Tejada, P. J., Gonzalez-Dugo, V., Berni, J., Sagardoy, R., Morales, F., & Fereres, E. (2010). Detecting water stress effects on fruit quality in orchards with time-series PRI airborne imagery. Remote Sensing of Environment, 114, 286-298.Swamy, K., Vardhini, B., Ramakrishna, B., Anuradha, S., Siddulu, N., & Rao, S. (2014). Role of 28-homobrassinolide on growth biochemical parameters of Trigonella foenugraecum L. plants subjected to lead toxicity. Multidisciplinary and Current Research, 2, 317. Upadhyay, P., & Maier, C. (2016). Effects of 17 β-estradiol on growth, primary metabolism, phenylpropanoid-flavonoid pathways and pathogen resistance in arabidopsis thaliana. Plant Science, 7, 1693-1710.Uprety, D. (2006). Interactive effect of moisture stress and elevated CO2 on the oxidative stress in brassica species. Journal of Food, Agriculture and Environmen, 4, 298-305. Valizadeh, M., Moharamnejad, S., Ahmadi, M., & Mohammadzadeh Jalaly, H. (2013). Changes in activity profile of some antioxidant enzymes in alfalfa half-sib families under salt stress. Journal of Agricultural Science and Technology, 15, 801-809. Xi, Z., Zhang, Z., Huo, S., Luan, L., Gao, X., Ma, L., & Fang, Y. (2013). Regulating the secondary metabolism in grape berry using exogenous 24-epibrassinolide for enhanced phenolics content and antioxidant capacity. Food chemistry, 141, 3056-3065.Yadava, P., Kaushal, J., Gautam, A., Parmar, H., & Singh, I. (2016). Physiological and biochemical effects of 24-epibrassinolide on heat-stress adaptation in maize (Zea mays L.). Natural Sciences, 8, 171. Zhu, F., Yun, Z., Ma, Q., Gong, Q., Zeng, Y., Xu, J., & Deng, X. (2015). Effects of exogenous 24-Epibrassinolide treatment on postharvest quality and resistance of Satsuma mandarin (Citrus unshiu). Postharvest Biology and Technology, 100, 8-15.
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Details
| Primary Language | English |
|---|---|
| Subjects | Horticultural Production |
| Journal Section | Articles |
| Authors | |
| Publication Date | June 30, 2020 |
| Acceptance Date | May 5, 2020 |
| Published in Issue | Year 2020 Volume: 30 Issue: 2 |
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Yuzuncu Yil University Journal of Agricultural Sciences by Van Yuzuncu Yil University Faculty of Agriculture is licensed under a Creative Commons Attribution 4.0 International License.