Year 2020, Volume 30 , Issue 3, Pages 611 - 619 2020-09-30

Türkiye’de Baklagil Nodüllerinden İzole Edilen Bitki Gelişimini Destekleyici Aktinobakterilerin Filogenisi
Phylogeny of Plant Growth-Promoting Actinobacteria Isolated from Legume Nodules in Turkey

Hilal AY [1]


Aktinobakteriler, tarımsal önemi olan moleküllerin yanı sıra birçok biyoaktif metabolitin üretimindeki işlevlerinden dolayı yararlanılan, biyoteknolojik olarak önemli bir mikroorganizma grubudur. Bu çalışmada, yabani baklagil nodüllerinden kültüre dayalı bir yaklaşımla aktinobakteriler izole edilmiş ve indol-3-asetik asit üretimi, atmosferik azot fiksasyonu ve inorganik fosfatı çözebilme özellikleri açısından bitki gelişimini destekleyici aktiviteleri araştırılmıştır. İzolatları tanımlamak amacıyla 16S rRNA gen dizi analizine dayanan bir moleküler yaklaşım kullanılmıştır. İkili dizi analizleri sonrasında altı izolatın Streptomyces ve Micromonospora cinslerinin üyeleri olduğu tespit edilmiştir. Bütün izolatlar indol-3-asetik asit üretebilmiş ve atmosferik azotu kullanabilmiştir. Ancak sadece bir izolat inorganik fosfatı çözünür hale getirebilmiştir. İzole edilen bu aktinobakteriler, özellikle atmosferik azotu kullanabilmeleri ve indol-3-asetik asit üretebilmeleri nedeniyle umut verici biyolojik gübre adayları olarak değerlendirilmektedir.
Actinobacteria are a biotechnologically important group of microorganisms utilized for their high capacity to synthesize many bioactive substances as well as agriculturally important compounds. In the present study, a culture-dependant approach was employed to isolate actinobacteria from wild legume nodules and their plant growth-promoting activities for indole-3-acetic acid production, atmospheric nitrogen fixation and inorganic phosphate solubilisation was investigated. A molecular approach based on 16S rRNA gene sequence analysis was employed to identify the isolates. After pairwise sequence analysis, six isolates were identified as members of the genera Streptomyces and Micromonospora. All isolates could produce indole-3-acetic acid and utilize atmospheric nitrogen while only one isolate was able to solubilize inorganic phosphate. The isolated actinobacteria are considered to be promising candidates for biological fertilizers especially because of their ability to use atmospheric nitrogen and produce high level of indole-3-acetic acid.
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Primary Language en
Subjects Science
Published Date Eylül 2020
Journal Section Articles
Authors

Orcid: 0000-0002-8735-4703
Author: Hilal AY (Primary Author)
Institution: ONDOKUZ MAYIS UNIVERSITY
Country: Turkey


Supporting Institution -
Project Number -
Thanks -
Dates

Publication Date : September 30, 2020

Bibtex @research article { yyutbd705227, journal = {Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi}, issn = {1308-7576}, eissn = {1308-7584}, address = {}, publisher = {Yuzuncu Yil University}, year = {2020}, volume = {30}, pages = {611 - 619}, doi = {10.29133/yyutbd.705227}, title = {Phylogeny of Plant Growth-Promoting Actinobacteria Isolated from Legume Nodules in Turkey}, key = {cite}, author = {Ay, Hilal} }
APA Ay, H . (2020). Phylogeny of Plant Growth-Promoting Actinobacteria Isolated from Legume Nodules in Turkey . Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi , 30 (3) , 611-619 . DOI: 10.29133/yyutbd.705227
MLA Ay, H . "Phylogeny of Plant Growth-Promoting Actinobacteria Isolated from Legume Nodules in Turkey" . Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi 30 (2020 ): 611-619 <https://dergipark.org.tr/en/pub/yyutbd/issue/56387/705227>
Chicago Ay, H . "Phylogeny of Plant Growth-Promoting Actinobacteria Isolated from Legume Nodules in Turkey". Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi 30 (2020 ): 611-619
RIS TY - JOUR T1 - Phylogeny of Plant Growth-Promoting Actinobacteria Isolated from Legume Nodules in Turkey AU - Hilal Ay Y1 - 2020 PY - 2020 N1 - doi: 10.29133/yyutbd.705227 DO - 10.29133/yyutbd.705227 T2 - Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi JF - Journal JO - JOR SP - 611 EP - 619 VL - 30 IS - 3 SN - 1308-7576-1308-7584 M3 - doi: 10.29133/yyutbd.705227 UR - https://doi.org/10.29133/yyutbd.705227 Y2 - 2020 ER -
EndNote %0 Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi Phylogeny of Plant Growth-Promoting Actinobacteria Isolated from Legume Nodules in Turkey %A Hilal Ay %T Phylogeny of Plant Growth-Promoting Actinobacteria Isolated from Legume Nodules in Turkey %D 2020 %J Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi %P 1308-7576-1308-7584 %V 30 %N 3 %R doi: 10.29133/yyutbd.705227 %U 10.29133/yyutbd.705227
ISNAD Ay, Hilal . "Phylogeny of Plant Growth-Promoting Actinobacteria Isolated from Legume Nodules in Turkey". Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi 30 / 3 (September 2020): 611-619 . https://doi.org/10.29133/yyutbd.705227
AMA Ay H . Phylogeny of Plant Growth-Promoting Actinobacteria Isolated from Legume Nodules in Turkey. YYÜ TAR BİL DERG. 2020; 30(3): 611-619.
Vancouver Ay H . Phylogeny of Plant Growth-Promoting Actinobacteria Isolated from Legume Nodules in Turkey. Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi. 2020; 30(3): 611-619.