Fungus Taksonomisinde DNA Barkodlamasının Kullanılması
Using DNA Barcoding in Fungal Taxonomy

Younes REZAEE DANESH [1] , Semra DEMİR [2]


Günümüzde toplam 1.5 milyon olduğu tahmin edilen fungus türünün %10’undan daha azı tanımlanmıştır. Moleküler biyolojideki son gelişmeler ve gen sıralama teknolojileri, çeşitli canlı organizma gruplarında, özellikle funguslarda yeni türlerin keşfedilmesini ve tanımlanmasını kolaylaştırmaktadır. Fungus türlerinin tanımlanmasında morfolojik yöntemler yeterli değildir, bu nedenle, hızlı ve doğru tanımlamada DNA verilerine dayalı yeni tekniklerin kullanılması ön plana çıkarılmıştır. DNA Barkodlama, fungus ve fungus benzeri mikroorganizmaları tespit etmek için kullanılan yıllık geçmişi olan yeni bir tekniktir. Ribozomal RNA genindeki ITS-rDNA uygun bir barkod dizisi olarak kabul edilmektedir. Bu bölge sekans verilerinin etkinliği, farklı mikologlar tarafından birçok fungusta standart bir barkod olarak kullanılmasına yol açmıştır. Bununla birlikte, ITS-rDNA bölgesinin fungus cins ve türlerinin tanılanmasında birincil barkod dizisi olarak kullanıldığı görülmekte, fungus gruplarındaki türe özgü kesin tanılama ise ikincil barkodlara dayalı olarak yapılmaktadır. Fungusların pleomorfik doğası nedeniyle, DNA barkodlamasının kullanılması çok önemlidir. Bugüne kadar, DNA barkodlama projeleri ile binlerce canlı organizma türü için yüz binlerce referans barkod dizisi oluşturulmuştur. Bilim adamlarının bundan sonraki çalışmaları tanımlama sürecini hızlandırmak için DNA barkodunun yönetimi ve otomasyonu yönündedir.

Totally, 1.5 million fungal species estimated, of which less than 10 percent have been described to date. Recent advances in molecular biology as well as gene sequencing technologies facilitate the discovery and identification of new species in various groups of living organisms, especially fungi. Morphological methods do not have efficiency for the identification of fungal species, so, using new techniques based on DNA data has been considered in the rapid and accurate fungal species identification. DNA Barcoding is a new technique with several years’ backgrounds for detecting fungi and fungal like organisms. The Internal Transcribed Spacer in ribosomal RNA gene (ITS-rDNA) has been considered as a suitable barcode sequence. The efficacy of this region’s sequence data in most fungal groups has led to its use as a standard barcode by different mycologists. However, the ITS-rDNA region appears to be used as the primary barcode sequence for fungi of the genus or species group. The accurate species-specific identification is performed depending on the fungal group based on secondary barcodes. Due to the pleomorphic nature of fungi, the use of DNA barcoding is of importance. To date, hundreds of thousands of reference barcode sequences have been created for thousands of species of living organisms through DNA barcoding projects. The subsequent efforts of scientists are managing and automation of DNA barcoding in order to speed up the identification process.

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Primary Language en
Subjects Plant Science
Published Date Aralık 2020
Journal Section Articles
Authors

Orcid: 0000-0003-1060-0264
Author: Younes REZAEE DANESH (Primary Author)
Institution: ANKARA TOPRAK, GUBRE VE SU KAYNAKLARI MERKEZ ARAŞTIRMA ENSTİTÜSÜ MÜDÜRLÜĞÜ
Country: Iran


Orcid: 0000-0002-0177-7677
Author: Semra DEMİR
Institution: VAN YÜZÜNCÜ YIL ÜNİVERSİTESİ, ZİRAAT FAKÜLTESİ, BİTKİ KORUMA BÖLÜMÜ
Country: Turkey


Dates

Publication Date : December 31, 2020

Bibtex @review { yyutbd751901, journal = {Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi}, issn = {1308-7576}, eissn = {1308-7584}, address = {}, publisher = {Yuzuncu Yil University}, year = {2020}, volume = {30}, pages = {989 - 997}, doi = {10.29133/yyutbd.751901}, title = {Using DNA Barcoding in Fungal Taxonomy}, key = {cite}, author = {Rezaee Danesh, Younes and Demi̇r, Semra} }
APA Rezaee Danesh, Y , Demi̇r, S . (2020). Using DNA Barcoding in Fungal Taxonomy . Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi , Volume:30 Issue: Additional issue , 989-997 . DOI: 10.29133/yyutbd.751901
MLA Rezaee Danesh, Y , Demi̇r, S . "Using DNA Barcoding in Fungal Taxonomy" . Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi 30 (2020 ): 989-997 <https://dergipark.org.tr/en/pub/yyutbd/issue/59373/751901>
Chicago Rezaee Danesh, Y , Demi̇r, S . "Using DNA Barcoding in Fungal Taxonomy". Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi 30 (2020 ): 989-997
RIS TY - JOUR T1 - Using DNA Barcoding in Fungal Taxonomy AU - Younes Rezaee Danesh , Semra Demi̇r Y1 - 2020 PY - 2020 N1 - doi: 10.29133/yyutbd.751901 DO - 10.29133/yyutbd.751901 T2 - Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi JF - Journal JO - JOR SP - 989 EP - 997 VL - 30 IS - Ek sayı (Additional issue) SN - 1308-7576-1308-7584 M3 - doi: 10.29133/yyutbd.751901 UR - https://doi.org/10.29133/yyutbd.751901 Y2 - 2020 ER -
EndNote %0 Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi Using DNA Barcoding in Fungal Taxonomy %A Younes Rezaee Danesh , Semra Demi̇r %T Using DNA Barcoding in Fungal Taxonomy %D 2020 %J Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi %P 1308-7576-1308-7584 %V 30 %N Ek sayı (Additional issue) %R doi: 10.29133/yyutbd.751901 %U 10.29133/yyutbd.751901
ISNAD Rezaee Danesh, Younes , Demi̇r, Semra . "Using DNA Barcoding in Fungal Taxonomy". Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi 30 / Ek sayı (Additional issue) (December 2020): 989-997 . https://doi.org/10.29133/yyutbd.751901
AMA Rezaee Danesh Y , Demi̇r S . Using DNA Barcoding in Fungal Taxonomy. YYÜ TAR BİL DERG. 2020; 30(Ek sayı (Additional issue)): 989-997.
Vancouver Rezaee Danesh Y , Demi̇r S . Using DNA Barcoding in Fungal Taxonomy. Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi. 2020; 30(Ek sayı (Additional issue)): 989-997.
IEEE Y. Rezaee Danesh and S. Demi̇r , "Using DNA Barcoding in Fungal Taxonomy", Yüzüncü Yıl Üniversitesi Tarım Bilimleri Dergisi, vol. 30, no. Ek sayı (Additional issue), pp. 989-997, Dec. 2021, doi:10.29133/yyutbd.751901