Hızlandırılmıs Yaşlandırma Yapılan Argomulyo Soybean (Glycine max (L.) Merr.) Mutant Hatlarına Ait Tohumların Depolanabirliği ve Genetik Parametrelerinin Belirlenmesi

Year 2021, Volume: 31 Issue: 3, 763 - 775, 15.09.2021

Siti Maesaroh , Yudıwantı Wahyu , Eny Wıdajatı

https://doi.org/10.29133/yyutbd.911571

Cited By: 1

Abstract

Gama ışını ile ışınlanmış olan Argomulyo popülasyonundan seçilen 22 soya fasulyesi mutant hatlarının tohum depolanabilirliği, APC-IPB 77-1MM olarak bilinen hızlı yaşlandırma aleti ile değerlendirilmiştir. Hızlandırılmış yaşlandırma testinde 20, 40, 60 ve 80 dakika içinde uygulanarak % 90 etanol kullanılmıştır. M100-96-53-6 'nın 20-80 dakika kimyasal hızlandırılmış yaşlandırma yapıldıktan sonra tohumun çimlenme oranı, çimlenme hızı ve elektriksel direnç değerinde yavaşlama eğilimi gösterilerek daha az tohum bozulma oranının yanı sıra iyi depolanabilirliğe sahip olduğu tahmin edilmiştir. Vigor (güç) testi için elektrik iletkenliği (EC) testi yerine bir alternatif olarak elektrik direnci (ER) testi kullanılabilmektedir. Nem içeriği hariç tüm parametrelerde bitkilerin kalıtım derecesi yüksek ve genetik ilerleme orta ila yüksek olduğu kaydedilmiştir. Regresyonun elde edilen R-kare değeri ve genetik parametreler değeri bakımından sonraki nesilde özellikleri iyileştirme amacıyla seçime (seleksiyona) ilk aşama için tohumun çimlenme oranı ve çimlenme hızı gözden geçirilebilmektedir.

Keywords

Etanol, Genetik, Canlılık, Baklagil, Vigor (Güç)

Seed Storability and Genetic Parameters Estimation on Accelerated Aging Seed of Argomulyo Soybean (Glycine max (L.) Merr.) Mutant Lines

Abstract

Seed storability of 22 selected soybean mutant lines from Argomulyo population irradiated by gamma ray were assessed by rapid aging tool, APC-IPB 77-1MM. Seed viability and vigor parameter observed due to genetic factors. The 90% ethanol was applied for 20, 40, 60 and 80 min as accelerated aging test. M100-96-53-6 was estimated that has good storability with less seed deterioration rate expressed by slow slope of germination percentage, germination speed and electrical resistance value after 20-80 min of chemical accelerated aging. Electrical resistance (ER) test could be as alternative and a substitute of electrical conductance (EC) test as alternative of vigor test. The high heritability and moderate to high genetic advance were noted on all parameters except moisture content. Germination percentage and germination speed could be reviewed for early stage selection to improve traits at the next generation based on R-square value of regresion and genetic parameters obtained.

Keywords

Ethanol, Genetic, Legume, Viability, Vigor

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