Novel g.2055A>C and g.2064T>A Polymorphisms of KISS1 Gene and Its Association with Reproductive Traits in Local Indonesian Goats

Year 2022, Volume: 32 Issue: 4, 766 - 774, 30.12.2022

Achiriah Febriana Edy Kurnianto Abu Bakar Sabir Sabir Yoyok Budi Pramono Sutopo Sutopo

https://doi.org/10.29133/yyutbd.1136214

Cited By: 1

Abstract

Kisspeptins are known as neuropeptides encoded by the KISS1 gene, which potentiates GnRH neuron excitability. Unfortunately, the role of the KISS1 gene in reproductive traits remains unclear in Indonesian native goat breeds. The current study purposed to detect the genetic variation of the KISS1 gene and investigate the association with reproductive traits in goats to acquire marker-assisted selection (MAS) for the breeding program. Further, ninety blood samples from randomly selected animals were used for DNA isolation and SNP genotyping. The blood serum was collected from sixteen treated goat does to assess FSH levels using ELISA method. The differences between studied parameters were determined using independent samples T-test from SAS Software. Two SNPs, g.2055A>C (SNP1) and g.2064T>A (SNP2) were discovered in intron 1 of KISS1 gene in Indonesian native goat breeds. Two genotypes were identified from each SNPs: AA and AC at SNP1 and TT and TA at SNP2. The allele A (0.96) at SNP1 and allele T (0.93) at SNP2 were discovered as dominant alleles in the population. Furthermore, chi-square test showed that both SNPs were under Hardy-Weinberg equilibrium. The variants of KISS1 gene have no effect on litter size (p>0.05). Moreover, AA genotype of SNP1 had a higher FSH level than that observed in AC genotype (p<0.0001), especially in the follicular phase. SNP2 does not correlate with FSH level relatively (p=0.23). Furthermore, using of AA genotype at SNP1 of KISS1 gene as MAS for the breeding selection program could escalate the reproductive traits in goats

Keywords

FSH, KISS1 gene, Litter size, SNP

Supporting Institution

Ministry of Agriculture of Indonesia and Diponegoro University

References

• Aerts, J. M. J., & Bols, P. E. J. (2010). Review article ovarian follicular dynamics. A review with emphasis on the bovine species. Part II: Antral development exogenous influence and future prospects. Reproduction in Domestic Animals, 45, 180-187. https://doi.org/10.1111/j.1439-0531.2008.01298.x
• Ali, A. T. (2015). Polycystic ovary syndrome and metabolic syndrome. Ceska Gynekologie, 80(4), 279-89.
• Alim, M. A., Hossain, M. M. K., Nusrat, J., Salimullah, M., Shu-Hong, Z., & Alam, J. (2019). Genetic effects of leptin receptor (LEPR) polymorphism on litter size in a Black Bengal goat population. Animal Biology, 69(4), 411–420. doi:10.1163/15707563-00001079
• An, X., Ma, T., Hou, J., Fang, F., Han, P., Yan, Y., Zhao, H., Song, Y., Wang, J., & Cao, B. (2013). Association analysis between variants in KISS1 gene and litter size in goats. BMC Genetics, 14(63), 1-6. https://doi.org/10.1186/1471-2156-14-63
• Bartlewski, P. M., Fuerst, K. J., Alexander, B. D., & King, W. A. (2009). Systemic concentrations of endogenous and exogenous FSH in anoestrous ewes superstimulated with Folltropin®-V. Reproduction in Domestic Animal, 44, 353-358. https://doi.org/10.1111/j.1439-0531.2008.01124.x
• Budisatria, I. G. S., Panjono, P., Agus, A., & Udo, H. M. J. (2012). The Productivity of Kejobong and Bligon goats, a local Indonesian goats kept by farmers. Proceedings of the 15th AAAP Animal Science Congress, November 26-30, Thammasat University, Thailand.
• Cao, G. L., Chu, M. X., Fang, L., Di, R., Feng, T., & Li, N. (2010). Analysis on DNA sequence of KiSS-1 gene and its association with litter size in goats. Molecular Biology Reports, 37, 3921-3929. https://doi.org/10.1007/s11033-010-0049-7
• de Roux, N., Genin, E., Carel, J.C., Matsuda, F., Chaussain, J. L., & Milgrom, E. (2003). Hypogonadotropic hypogonadism due to loss of function of the KiSS1-derived peptide receptor GPR54. Proceeding National Academic Science, 100, 10972–10976. https://doi.org/10.1073/pnas.1834399100
• Dumalska, I., Wu, M., Morozova, E., Liu, R., Van den Pol, A., & Alreja, M. (2008). Excitatory effects of the puberty-initiating peptide kisspeptin and group I metabotropic glutamate receptor agonists differentiate two distinct subpopulations of gonadotropin-releasing hormone neurons. Journal of Neuroscience, 28, 8003–8013. DOI: https://doi.org/10.1523/JNEUROSCI.1225-08.2008
• El-Tarabany, M. S., Zagloola, A. W., El-Tarabany, A. A., & Awad, A. (2017). Association analysis of polymorphism in KiSS1 gene with reproductive traits in goats. Animal Reproduction. Science, 180, 92–99. https://doi.org/10.1016/j.anireprosci.2017.03.006
• Excoffier, L., & Lischer, H. E. L. (2010). Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Molecular Ecology Resources, 10, 564–567. https://doi.org/10.1111/j.1755-0998.2010.02847.x
• Febriana, A., Sutopo, S., Kurnianto, E., & Widiyanto, W. (2021). Phylogenetic study and association between prominent genotype and haplotype of KISS1 gene with FSH Level in Indonesian native goat breeds. Journal of the Indonesian Tropical Animal Agriculture, 46(4), 282-294. https://doi.org/10.14710/jitaa.46.4.282-294
• Febriana, A., Sutopo, S., Kurnianto, E., & Widiyanto, W. (2022). A novel SNPs of KISS1 gene strongly associated with litter size in Indonesian goat breeds. Tropical Animal Science Journal (Accepted).
• Fleming, T. S., Greenwood, P. J., Heath, D. A., Hudson, N. L., Lun, S., Shaw, L., & McNatty, K. P. (1996). Expression of gonadotrophin subunit genes in sheep that were homozygous carrier and non-carrier of the Booroola fecundity gene FecB. Journal of Reproduction and Fertility, 103, 315-321.
• Funes, S., Hedrick, J. A., Vassileva, G., Markowitz, L., Abbondanzo, S., Golovko, A., Yang, S., Monsma, F. J., & Gustafson, E. L. (2003). The KiSS-1 receptor GPR54 is essential for the development of the murine reproductive system. Biochemical and Biophysical Research Communications, 312, 1357–1363. https://doi.org/10.1016/j.bbrc.2003.11.066
• Han, S. K., Gottsch, M. L., Lee, K. J., Popa, S. M., Smith, J. T., Jakawich, S. K., Clifton, D. K., Steiner, R. A., & Herbison, A. E. (2005). Activation of gonadotropin-releasing hormone (GnRH) neurons by kisspeptin as a neuroendocrine switch for the onset of puberty. Journal of Neuroscience, 25, 11349–11356. DOI: https://doi.org/10.1523/JNEUROSCI.3328-05.2005
• Hardyta, G., Widayati, D. T., & Maharani, D. (2020). Association of SNP T125A on KiSS1 gene with reproduction hormone levels in Kaligesing goat. Journal of the Indonesian Tropical Animal Agriculture, 45(4), 253-260. https://doi.org/10.14710/jitaa.45.4.253-260
• Herbison, A. E., De Tassigny, X., Doran, J., & Colledge, W. H. (2010). Distribution and postnatal development of Gpr54 gene expression in mouse brain and gonadotropin-releasing hormone neurons. Endocrinolog, 151, 312–321. https://doi.org/10.1210/en.2009-0552
• Hou, J. X., An, X. P., Wang, J. G., Song, Y. X., Cui, Y. H., Wang, Y. F., Chen, Q. J., & Cao, B. Y. (2011). New genetic polymorphisms of KiSS-1 gene and their association with litter size in goats. Small Ruminant Research, 96, 106–110. https://doi.org/10.1016/j.smallrumres.2010.11.013
• Irwig, M. S., Fraley, G. S., Smith, J. T., Acohido, B. V., Popa, S. M., Cunningham, M. J., Gottsch, M. L., Clifton, D. K., & Steiner, R. A. (2004). Kisspeptin activation of gonadotropin releasing hormone neurons and regulation of KiSS-1 mRNA in the male rat. Neuroendocrinology, 80, 264–272. https://doi.org/10.1159/000083140
• Janssens, S., Vandepitte, W., & Bodin, L. (2004). Genetic parameters for LS in sheep: natural versus hormone-induced oestrus. Genetics Selection Evolution, 36, 543–562. https://doi.org/10.1051/gse:2004016
• Jeet, V., Magotraa, A., Banga, Y. C., Kumar, S., Garga, A. R., Yadava, A. S., & Bahurupi, P. (2022). Evaluation of candidate point mutation of Kisspeptin 1 gene associated with litter size in Indian Goat breeds and its effect on transcription factor binding sites. Domestic Animal Endocrinology, 78, 106676. https://doi.org/10.1016/j.domaniend.2021.106676
• Kirilov, M., Clarkson, J., Liu, X., Roa, J., Campos, P., Porteous, R., Schu¨tz, G., & Herbison, A. E. (2013). Dependence of fertility on kisspeptin–Gpr54 signaling at the GnRH neuron. Nature Communications, 4, 2492. DOI: 10.1038/ncomms3492
• Li, B., Krishnan, V. G., Mort, M. E., Xin, F., Kamati, K. K., & Cooper, D. N. (2009). Automated inference of molecular mechanisms of disease from amino acid substitutions. Bioinformatics, 25, 2744–2750. https://doi.org/10.1093/bioinformatics/btp528
• Liu, X., Lee, K., & Herbison, A. E. (2008). Kisspeptin excites gonadotropin-releasing hormone neurons through a phospholipase C/calcium-dependent pathway regulating multiple ion channels. Endocrinology, 149, 4605–4614. https://doi.org/10.1210/en.2008-0321
• Mayer, C., & Boehm, U. (2011) Female reproductive maturation in the absence of kisspeptin/GPR54 signaling. Nature Neuroscience, 14,704–710. doi:10.1038/nn.2818
• McGee, E. A., & Hsueh, A. J. (2000). Initial and cyclic recruitment of ovarian follicles. Endocrine Reviews, 21(2000), 200-214. https://doi.org/10.1210/edrv.21.2.0394
• Mekuriaw, G., Mwacharo, J. M., Dessie, T., Mwai, O., Djikeng, A., Osama, S., Gebreyesus, G., Kidane, A., Abega, S., & Tesfaye, K. (2017). Polymorphism analysis of kisspeptin (KISS1) gene and its association with litter size in Ethiopian indigenous goat populations. African Journal of Biotechnology, 16(22), 1254-1264. DOI: 10.5897/AJB2016.15750.
• Mulyono, R. H., Sumantri, C., Noor, R. R., Jakaria J., & Astuti, D. A. (2019). Analisis keterkaitan gen BMP15, BMPR1B dan KISS1 dengan sifat fekunditas pada kambing Peranakan Etawah betina. Jurnal Ilmu Pertanian Indonesia, 24(2), 83-92. https://doi.org/10.18343/jipi.24.2.83
• Nei, M. & Kumar, S. (2000). Molecular Evaluation and Phylogenetiks. Oxford University Press, New York.
• Nett, W. J., Oloff, S. H., & McCarthy, K. D. (2002). Hippocampal astrocytes in situ exhibit calcium oscillations that occur independent of neuronal activity. Journal of Neurophysiology, 87(1), 528–537. https://doi.org/10.1152/jn.00268.2001
• Notter, D. R. (2012). Genetic improvemnet of reproductive efficiency of sheep and goats. Animal Reproduction Science, 130, 147-151. https://doi.org/10.1016/j.anireprosci.2012.01.008
• Olsen, I., Gjerde, B., & Groen, A. F. (1999). Accommodation and evaluation of ethical, strategic and economic values in animal breeding goals. Book of Abstracts No. 5 European Federation of Animal Science. pp. 33
• Peacocka, C., & Sherman, D. M. (2010). Sustainable goat production—Some global perspectives. Small Ruminant Research, 89:70–80. https://doi.org/10.1016/j.smallrumres.2009.12.029
• Pielecka-Fortuna, J., Chu, Z., & Moenter, S. M. (2008). Kisspeptin acts directly and indirectly to increase gonadotropin-releasing hormone neuron activity and its effects are modulated by estradiol. Endocrinology, 149, 1979–1986. https://doi.org/10.1210/en.2007-1365
• Pinilla, L., Aguilar, E., Diegue, C., Millar, R. P., & Tena-Sempere, M. (2012). Kisspeptins and reproduction: physiological roles and regulatory mechanisms. Physiological Reviews, 92, 1235–1316. https://doi.org/10.1152/physrev.00037.2010
• Redden, R., & Thorne, J. W. (2020). Sheep and Goat Production. Animal Agriculture: Sustainability, Challenges and Innovations. Texas A&M AgriLife Extension. USA. https://doi.org/10.1016/B978-0-12-817052-6.00012-4
• Sahoo, S. S., Mishra, C., Kaushik, R., Rout, P. K., Singh, M. K., Bhusan, S., & Dige, M. S. (2019). Association of a SNP in KISS 1 gene with reproductive traits in goats. Biological Rhythm Research, 23, 1-12. https://doi.org/10.1080/09291016.2019.1608730
• Sarma, L., Nahardeka, N., Zaman, G., Aziz, A., Das, A., Akhtar, F., Upadhyay, S., & Borkolita, L. (2019). Analysis of BMP4 gene HaeIII polymorphism in Assam Hill goat. Journal of Entomology and Zoology Studies, 7(2), 34-37.
• SAS. (2018). SAS® University Edition software 3.8. Cary, North Carolina.
• Singh, P. P., Tomar, S. S., Thakur, M. S., & Kumar, A. (2015). Polymorphism and association of Growth Hormone gene with growth traits in Sirohi and Barbari breeds of goat. Veterinary World, 8(3), 382-387. doi: 10.14202/vetworld.2015.382-387.
• Tu, P. A., Lo, L. L., Chen, Y. C., Hsu, C. C., Shiau, J. W., Lin, E. C., Lin, R. S., & Wang, P. H. (2014). Polymorphisms in the promoter region of myostatin gene are associated with carcass traits in pigs. Journal of Animal Breeding and Genetics, 131, 116–122. https://doi.org/10.1111/jbg.12053
• Wang, J. J., Li, Z. D., Zheng, L. Q., Zhang, T., Shen, W., & Lei, C. Z. (2022). Genome-wide detection of selective signals for fecundity traits in goats (Capra hircus). Gene, 818, 146221. https://doi-org.proxy.undip.ac.id/10.1016/j.gene.2022.146221
• Wikins, J. F. (1997). Method of stimulating ovulation rate in ewes may affect conception but not embryo survival. Animal Reproduction Science, 47, 31-42. https://doi.org/10.1016/S0378-4320(96)01636-3
• Zhang, C., Roepke, T. A., Kelly, M. J., & Ronneklei, O. K. (2008). Kisspeptin depolarizes gonadotropin-releasing hormone neurons through activation of TRPC-like cationic channels. Journal of Neuroscience, 28, 4423–4434. https://doi.org/10.1523/JNEUROSCI.5352-07.2008
• Zhu, S., Zhang, B., Jiang, X., Li, Z., Zhao, S., Cui, L., & Chen, Z. (2019). Metabolic disturbances in nonobese women with polycystic ovary syndrome: a systematic review and meta-analysis. Fertility and Sterility, 111(1), 168-177. https://doi.org/10.1016/j.fertnstert.2018.09.013