In-vitro and in-vivo Antimicrobial Properties of Pomegranate Peel Extract Genotypes against Bacterial Vaginosis in bovine

Year 2022, Volume: 32 Issue: 4, 825 - 834, 30.12.2022

Elmira Ziya Motalebıpour , Akbar Pirestani

https://doi.org/10.29133/yyutbd.1167392

Abstract

Drug resistance against bovine bacterial vaginosis (BV) in common treatments needs new therapeutic agents from other sources. Many plants demonstrate antimicrobial properties that could control pathogenic microorganisms. Uterus infections in bovine are associated with calving difficulty, retained placenta, and overgrowth of pathogenic microorganisms in the reproductive tract. This study examined the antibacterial effects of pomegranate peel extracts against various bacterial vaginosis in bovine. Methanolic and aqueous extracts of different pomegranate peels were prepared. An antibiogram test was performed against nine various bacterial vaginosis of bovine. Then, inhibitory concentration values were determined for pomegranate peel extracts (100, 200, and 400 mg/ml). In in-vivo observation, the greatest inhibition zone activated pink pomegranate peel extracts (8 mg/ml) prepared by methanolic extracts. The serial dilution tests indicated that the bactericidal effect of high-concentration methanolic extract was more than those of low-concentration types. Experimental treatments in in-vitro observation constituted including (treated, T) with 180 cows that encountered difficulty in birth and non-including (control, C) with 30 cows with no treatments after vaginal problems. The effect of various pomegranate peel extract (PPE) concentrations increased during the treatment days. All the treated Holstein cows with nearly 400mg/ml of PPE gel (both methanolic and aqueous extracts methods) were recovered after 4 days. The results of this study showed the PPE gel effectiveness in the pharmaceutical industry.

Keywords

Antibacterial effect, Punica granatum L., Vaginal drug

Supporting Institution

The authors are thankful to the Director of the Transgenesis Center of Excellence, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran and Medicinal plants research center, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran for providing all the research facilities during this study.

Project Number

This work was supported by the Technology Incubator Center of Medicinal Plant of Isfahan (Khorasgan) Branch, Islamic Azad University.

Thanks

The authors are thankful to the Director of the Transgenesis Center of Excellence, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran and Medicinal plants research center, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran for providing all the research facilities during this study.

References

• Alexandre, E. M., Silva, S., Santos, S. A., Silvestre, A. J., Duarte, M. F., Saraiva, J. A., & Pintado, M. (2019). Antimicrobial activity of pomegranate peel extracts performed by high pressure and enzymatic assisted extraction. Food research international, 115, 167-176. doi: 10.1016/j.foodres.2018.08.044
• Al-Saimary, I. E., Bakr, S. S., Jaffar, T., Al-Saimary, A. E., Salim, H. H., & Al-Muosawi, R. (2002). Effects of some plant extracts and antibiotics on Pseudomonas aeruginosa isolated from various burn cases. Saudi medical journal, 23(7), 802-805.
• Al-Zoreky, N. S. (2009). Antimicrobial activity of pomegranate (Punica granatum L.) fruit peels. International journal of food microbiology, 134(3), 244-248. doi: 10.1016/j.ijfoodmicro.2009.07.002.
• Bauer, A. W. (1966). Antibiotic susceptibility testing by a standardized single disc method. American Journal of Clinical Pathology, 45:149-58.
• Carneiro, L. C., Cronin, J. G., & Sheldon, I. M. (2016). Mechanisms linking bacterial infections of the bovine endometrium to disease and infertility. Reproductive biology, 16(1), 1-7. doi: 10.1016/j.repbio.2015.12.002
• Casquete, R., Castro, S. M., Martín, A., Ruiz-Moyano, S., Saraiva, J. A., Córdoba, M. G., & Teixeira, P. (2015). Evaluation of the effect of high pressure on total phenolic content, antioxidant and antimicrobial activity of citrus peels. Innovative Food Science & Emerging Technologies, 31, 37-44. doi: 10.1016/j.ifset.2015.07.005
• Coleman, D. A., Thayne, W. V., & Dailey, R. A. (1985). Factors affecting reproductive performance of dairy cows. Journal of dairy science, 68(7), 1793-1803. doi: 10.3168/jds.S0022-0302(85)81029-8.
• Dahham, S. S., Ali, M. N., Tabassum, H., & Khan, M. (2010). Studies on antibacterial and antifungal activity of pomegranate (Punica granatum L.). Am. Eurasian J. Agric. Environ. Sci, 9(3), 273-281.
• Elshafie, H. S., Caputo, L., De Martino, L., Sakr, S. H., De Feo, V., & Camele, I. (2021). Study of bio-pharmaceutical and antimicrobial properties of pomegranate (Punica granatum L.) leathery exocarp extract. Plants, 10(1), 153. doi: 10.3390/plants10010153.
• George, N. S., Cheung, L., Luthria, D. L., Santin, M., Dawson, H. D., Bhagwat, A. A., & Smith, A. D. (2019). Pomegranate peel extract alters the microbiome in mice and dysbiosis caused by Citrobacter rodentium infection. Food science & nutrition, 7(8), 2565-2576. doi: 10.1002/fsn3.1106.
• Heber, D., Schulman, R. N., & Seeram, N. P. (Eds.). (2006). Pomegranates: ancient roots to modern medicine. CRC press.
• Holetz, F. B., Pessini, G. L., Sanches, N. R., Cortez, D. A. G., Nakamura, C. V., & Dias Filho, B. P. (2002). Screening of some plants used in the Brazilian folk medicine for the treatment of infectious diseases. Memórias do Instituto Oswaldo Cruz, 97, 1027-1031. doi: 10.1590/S0074-02762002000700017.
• Ismail, T., Akhtar, S., Sestili, P., Riaz, M., Ismail, A., & Labbe, R. G. (2016). Antioxidant, antimicrobial and urease inhibitory activities of phenolics‐rich pomegranate peel hydro‐alcoholic extracts. Journal of Food Biochemistry, 40(4), 550-558. doi: 10.1111/jfbc.12250
• Ismail, T., Sestili, P., & Akhtar, S. (2012). Pomegranate peel and fruit extracts: a review of potential anti-inflammatory and anti-infective effects. Journal of ethnopharmacology, 143(2), 397-405. doi:10.1016/j.jep.2012.07.004.
• Jami, E., Shabtay, A., Nikbachat, M., Yosef, E., Miron, J., & Mizrahi, I. (2012). Effects of adding a concentrated pomegranate-residue extract to the ration of lactating cows on in vivo digestibility and profile of rumen bacterial population. Journal of Dairy Science, 95(10), 5996-6005. doi: 10.3168/jds.2012-5537
• Jardines, M., & Alcoba, R. (2020). U.S. Patent Application No. 16/391,292.
• Joesoef, M. R., Schmid, G. P., Hillier, S. L. Bacterial vaginosis: review of treatment options and potential clinical indications for therapy. Clinical Infectious Diseases 1999; 28(Supplement_1):S57-65. doi: 10.1086/514725
• Jurenka, J. (2008). Therapeutic applications of pomegranate (Punica granatum L.): a review. Alternative medicine review, 13(2).
• Kantachumpoo, A., & Chirapart, A. (2010). Components and antimicrobial activity of polysaccharides extracted from Thai brown seaweeds. Agriculture and Natural Resources, 44(2), 220-233.
• Klebanoff, M. A., Hauth, J. C., MacPherson, C. A., Carey, J. C., Heine, R. P., Wapner, R. J., Iams, J. D., Moawad, A., Miodovnik, M., Sibai, B. M., Peter vanDorsten, J. (2004). Time course of the regression of asymptomatic bacterial vaginosis in pregnancy with and without treatment. American Journal of Obstetrics and Gynecology, 190(2), 363-370.
• Lansky, E. P., & Newman, R. A. (2007). Punica granatum (pomegranate) and its potential for prevention and treatment of inflammation and cancer. Journal of ethnopharmacology, 109(2), 177-206. doi: 10.1016/j.jep.2006.09.006.
• Lewis, G. S. (1997). Uterine health and disorders. Journal of dairy science, 80(5), 984-994. doi: 10.3168/jds.S0022-0302(97)76024-7
• Machado, T. D. B., Leal, I. C., Amaral, A. C. F., Santos, K., Silva, M. G. D., & Kuster, R. M. (2002). Antimicrobial ellagitannin of Punica granatum fruits. Journal of the Brazilian Chemical Society, 13, 606-610. doi: 10.1590/S0103-50532002000500010.
• Meléndez, P. A., & Capriles, V. A. (2006). Antibacterial properties of tropical plants from Puerto Rico. Phytomedicine, 13(4), 272-276. doi: 10.1016/j.phymed.2004.11.009.
• Miguel, M. G., Neves, M. A., & Antunes, M. D. (2010). Pomegranate (Punica granatum L.): A medicinal plant with myriad biological properties-A short review. Journal of Medicinal Plants Research, 4(25), 2836-2847. doi: 10.5897/JMPR.9001028.
• Naz, S., Siddiqi, R., Ahmad, S., Rasool, S. A., & Sayeed, S. A. (2007). Antibacterial activity directed isolation of compounds from Punica granatum. Journal of food science, 72(9), M341-M345. doi: 10.1111/j.1750-3841.2007.00533.x.
• Naziri, Z., Rajaian, H., & Firouzi, R. (2012). Antibacterial effects of Iranian native sour and sweet pomegranate (Punica granatum) peel extracts against various pathogenic bacteria.
• Nisar, B., Sultan, A., & Rubab, S. L. (2018). Comparison of medicinally important natural products versus synthetic drugs-a short commentary. Nat. Prod. Chem. Res, 6(2), 308. doi: 10.4172/2329-6836.1000308.
• Okunji, C. O., Okeke, C. N., Gugnani, H. C., & Iwu, M. M. (1990). An antifungal spirostanol saponin from fruit pulp of Dracaena mannii. International Journal of Crude Drug Research, 28(3), 193-199. doi: 10.3109/13880209009082811
• Oliveira, R. A., Narciso, C. D., Bisinotto, R. S., Perdomo, M. C., Ballou, M. A., Dreher, M., & Santos, J. E. P. (2010). Effects of feeding polyphenols from pomegranate extract on health, growth, nutrient digestion, and immunocompetence of calves. Journal of Dairy Science, 93(9), 4280-4291.doi: 10.3168/jds.2010-3314.
• Perricone, V., Comi, M., Giromini, C., Rebucci, R., Agazzi, A., Savoini, G., & Bontempo, V. (2020). Green tea and pomegranate extract administered during critical moments of the production cycle improves blood antiradical activity and alters cecal microbial ecology of broiler chickens. Animals, 10(5), 785. doi: 10.3390/ani10050785.
• Prashanth, D., Asha, M. K., & Amit, A. (2001). Antibacterial activity of Punica granatum. Fitoterapia, 72(2), 171-173. doi: 10.1016/s0367-326x(00)00270-7.
• Reddy, M. K., Gupta, S. K., Jacob, M. R., Khan, S. I., & Ferreira, D. (2007). Antioxidant, antimalarial and antimicrobial activities of tannin-rich fractions, ellagitannins and phenolic acids from Punica granatum L. Planta medica, 53(05), 461-467. doi: 10.1055/s-2007-967167.
• Ricci, D., Giamperi, L., Bucchini, A., & Fraternale, D. (2006). Antioxidant activity of Punica granatum fruits. Fitoterapia, 77(4), 310-312.
• Ross, J. D. C. (2002). An update on pelvic inflammatory disease. Sexually transmitted infections, 78(1), 18-19. doi:10.1136/sti.78.1.18.
• Sheldon, I. M., Lewis, G. S., LeBlanc, S., & Gilbert, R. O. (2006). Defining postpartum uterine disease in cattle. Theriogenology, 65(8), 1516-1530.
• Sheldon, I. M., Noakes, D. E., Rycroft, A. N., Pfeiffer, D. U., & Dobson, H. (2002). Influence of uterine bacterial contamination after parturition on ovarian dominant follicle selection and follicle growth and function in cattle. REPRODUCTION-CAMBRIDGE-, 123(6), 837-845.
• Voravuthikunchai, S. P., Sririrak, T., Limsuwan, S., Supawita, T., Iida, T., & Honda, T. (2005). Inhibitory effects of active compounds from Punica granatum pericarp on verocytotoxin production by enterohemorrhagic Escherichia coli O157: H7. Journal of health science, 51(5), 590-596. doi.org/10.1248/jhs.51.590.
• Williams, E. J., Fischer, D. P., Pfeiffer, D. U., England, G. C., Noakes, D. E., Dobson, H., & Sheldon, I. M. (2005). Clinical evaluation of postpartum vaginal mucus reflects uterine bacterial infection and the immune response in cattle. Theriogenology, 63(1), 102-117. doi: 10.1016/j.theriogenology.2004.03.017.
• Yudin, M. H., Landers, D. V., Meyn, L., & Hillier, S. L. (2003). Clinical and cervical cytokine response to treatment with oral or vaginal metronidazole for bacterial vaginosis during pregnancy: a randomized trial. Obstetrics & Gynecology, 102(3), 527-534. doi: 10.1016/s0029-7844(03)00566-0.