Examination of Shallot Seedling Quality Through Seed Priming
Year 2025,
Volume: 35 Issue: 1, 44 - 52
Abdul Jalil
,
Elkawakib Syam'un
,
Syatrianty Andi Syaiful
Abstract
The study aimed to examine the effect of the seed priming method with zinc micronutrients on the growth of seedlings of two shallot varieties from aging botanical seeds. The research was conducted in the Green House, Agriculture Faculty, Hasanuddin University, Makassar, Indonesia. The study was arranged in a split-plot design (SPD) with a randomized complete block design (RCBD). The main plot was a type of variety, which consisted of 2 combinations, namely Lokananta and Maserati. The subplot was a type of priming comprising six treatments: unpriming, hydropriming, IAA priming, ZnO priming, ZnSO4·7H2O priming, and Zn-EDTA priming. Zn-EDTA priming increased germination percentage (71.43%), root length (13.07 cm), and number of root tips (12.04). ZnSO4.7H2O priming produced the highest seedling height (28.72 cm). Then, ZnO priming increased the number of leaves (4.18 leaves), pseudo-stem diameter (3.36 mm), fresh weight (2.54 g), dry weight (0.22 g), and seedling quality index (0.0081). Seed priming using Zn improved the growth quality of aging shallot seedlings.
Ethical Statement
Ethical approval is not required for this study because this research does not cause any negative impacts that are harmful to human life and the environment.
Supporting Institution
Indonesian Ministry of Education, Culture, Research and Technology
Project Number
0667/E5/AL.04/2024
Thanks
The authors would like to thank the Indonesian Ministry of Education, Culture, Research and Technology for supporting the research costs through the Fundamental Research Grant Scheme (BIMA).
References
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- Choukri, M., Abouabdillah, A. A., Bouabid, R. A., Abd-Elkader, O. H. B., Pacioglu, O. H. C., Boufahja, F. D., & Bourioug, M. (2022). Zn application through seed priming improves productivity and grain nutritional quality of silage corn. Saudi Journal of Biological Sciences, 29(103456), 1-9. https://doi.org/10.1016/j.sjbs.2022.103456
- Demirkaya, M., Dietz, K. J., & Sivritepe, H. O. (2010). Changes in antioxidant enzymes during ageing of onion seeds. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 38(1), 49-52. https://doi.org/10.15835/nbha3814575
- Faried, M., Syam'un, E., & Mantja, K. (2023). Survival rate, disease incidence, and yield of shallots by seed priming and application of tithonia compost enriched with gliocladium virens. Int. J. of Life Sciences and Agriculture Research, 2(5), 57-62. https://doi.org/10.55677/ijlsar/V02I05Y2023-03
- Faried, M., Syam’un, E., Mantja, K. (2024a). Dissimilarities of shallot (Allium ascalonicum L.) seedlings growth and quality through priming with moringa leaf extract. Kufa Journal for Agriculture Science, 16(2), 18-29.
- Faried, M., Syam’un, E., Jalil, A., Cennawati, C., Wijaya, P., & Putri, R. W. (2024b). Can pruning affect the growth of shallot (Allium ascalonicum L.) seedlings from seeds?. Journal of Agricultue Faculty of Ege University, 61(2), 165-174.
- Farooq, M., Romdhane, L., Rehman, A., Al-Alawi, A. K. M., Al-Busaidi, W. M., Asad, S. A., & Lee, D. J. (2020). Integration of seed priming and biochar application improves drought tolerance in cowpea. J. Plant Growth Regul, 1-9. https://doi.org/10.1007/ s00344-020-10245-7.
- Farooq, M. A., Zhang, X., Zafar, M. M., Ma, W., & Zhao, J. (2021). Roles of reactive oxygen species and mitochondria in seed germination. Front. Plant Sci, 12(781734). https://doi.org/10.3389/fpls.2021.781734
- Finch Savage, W. E., & Bassel, G. W. (2016) Seed vigor and crop establishment: Extending performance beyond adaptation. J. Exp. Bot, 67(3), 567–591. https://doi.org/10.1093/jxb/erv490
- Freitas, M. N., Guerra, M. B. B., Adame, A., Moraes, T. F., Junior, J. L., Pérez, C. A., Abdala, D. B., & Cicero, S. M. (2020). A first glance at the micro-ZnO coating of maize (Zea mays L.) seeds: A study of the elemental spatial distribution and Zn speciation analysis. J. Anal. At. Spectrom, 35, 3021–3031. https://doi.org/10.1039/D0JA00282H
- Hassan, U. M., Aamer, M., Chattha, U. M., Haiying, T., Shahzad, B., Barbanti, L., Nawaz, M., Rasheed, A., Afzal, A., Liu, Y., & Guoqin, H. (2020). The critical role of zinc in plants facing the drought stress. Agriculture, 10(9)396, 1-20. https://doi.org/10.3390/agriculture10090396
- Hourston, J. E., Pérez, M., Gawthrop, F., Richards, M., Steinbrecher, T., & Leubner-Metzger, G. (2020). The effects of high oxygen partial pressure on vegetable Allium seeds with a short shelf-life. Planta, 251(105), 1-9. https://doi.org/10.1007/s00425-020-03398-y
- Imran, M., Mahmood, A., Neumann, G., & Boelt, B. (2021). Zinc seed priming improves spinach germination at low temperature. Agriculture, 11(271), 1-12. https://doi.org/10.3390/agriculture11030271
- Kumar, R. J., Pandey, S., & Bose, B. (2021). Seed priming with Mg(NO3)2 and ZnSO4 salts triggers physio-biochemical and antioxidant defense to induce water stress adaptation in wheat (Triticum aestivum L.). Plant Stress, 2(100037), 1-12. https://doi.org/10.1016/j.stress.2021.100037
- Małecka, A., Ciszewska, L., Staszak, A., & Ratajczak, E. (2021). Relationship between mitochondrial changes and seed aging as a limitation of viability for the storage of beech seed (Fagus sylvatica L.). PeerJ, 9(e10569), 1-13. http://doi.org/10.7717/peerj.10569
- Mohammed, W., Woldetsadik, K., & Kabede, B. (2018). Registration of a new improve huruta shallot variety with true seed production potensial. East African Journal of Sciences, 12(1), 77-82.
- Pagano, A., Macovei, A., Xia, X., Padula, G., Hołubowicz, R., Balestrazzi, A. (2023). Seed priming applied to onion-like crops: state of the art and open questions. Agronomy, 13, 288. https://doi.org/10.3390/agronomy13020288
- Pangestuti, R., Sulistiyaningsih, E., Kurniasi, B., & Murti, R. H. (2021). Improving seed germination and seedling growth of true seed shallot (TSS) using plant growth regulator seed priming. In IOP Conference Series: Earth and Environmental Science, 883(012024), 1-8. https://doi.org/10.1088/1755-1315/883/1/012024
- Pehlivan, F. E. (2017). Free radicals and antioxidant system in seed biology. In J.C. Jimenez-Lopez (Ed.), Advances in Seed Biology (pp. 167-183). InTech. http://dx.doi.org/10.5772/intechopen.70837
- Poudel, P., Gioia, D. F., Lambert, J. D., & Connolly, E. L. (2023). Zinc biofortification through seed nutri-priming using alternative zinc sources and concentration levels in pea and sunflower microgreens. Front. Plant Sci, 14(1177844). https://doi.org/10.3389/fpls.2023.1177844
- Rehman, A., Farooq, M., Naveed, M., Nawaz, A., & Shahzad, B. (2018a). Seed priming of Zn with endophytic bacteria improves the productivity and grain biofortification of bread wheat. Eur. J. Agron, 218, 98–107. https://doi.org/10.1016/j.eja.2018.01.017
- Rehman, A., Farooq, M., Naveed, M., Ozturk, L., & Nawaz, A. (2018b). Pseudomonas-aided zinc application improves the productivity and biofortification of bread wheat. Crop Pasture Sci, 69(7), 659–672. https://doi.org/10.1071/CP17441
- Rehman, A., Farooq, M., Ozturk, L., Asif, M., & Siddique, K. H. M. (2018c). Zinc nutrition in wheat-based cropping systems. Plant Soil, 422(1-2), 283–315. https://doi.org/10.1007/s11104-017-3507-3
- Rehman, A., Farooq, M., Asif, M., & Ozturk, L. (2019). Supra-optimal growth temperature exacerbates adverse effects of low Zn supply in wheat. J. Plant Nutr. Soil Sci, 182(4), 656–666. https://doi.org/10.1002/jpln.201800654
- Saranya, N., Renugadevi, J., Raja, K., Rajashree, V., & Hemalatha, G. (2017). Seed priming studies for vigour enhancement in onion CO onion (5). J. Pharm. Phytochem. 6(3), 77–82.
- Schober, P., Boer, C., & Schwarte, L. (2018). Correlation coefficient: Appropriate use and interpretation. Anesthesia and Analgesia, 126(5), 1763-1768. https://doi.org/10.1213/ANE.0000000000002864
- Seddigh, M., Hossein, A., Goftarmanesh, K., & Ghasemi, S. (2016). The effectiveness of seed priming with synthetic zinc-amino acid chelates in comparison with soil-applied ZnSO4 in improving yield and zinc availability of wheat grain. J. Plant Nutr, 39(3), 417–427. https://doi.org/10.1080/01904167.2015.1069340
- Sivritepe, H. O., & Demirkaya, M. (2012). Does humidification technique accomplish physiological enhancement better than priming in onion seeds?. Acta Horticulturae, 960, 237-244. https://doi.org/10.17660/ActaHortic.2012.960.34
- Syam’un, E., Mantja, K., Ulfa, F., Junaid, M., Jayadi, M., Sjam, S., Said, M. I., Suhardi, S., & Syamsia, S. (2024). Influence of variety, beneficial fungi, and application on the growth and production of shallot (Allium ascalonicum L.). Yuzuncu Yil University Journal of Agricultural Sciences, 34(4), 559-570. https://doi.org/10.29133/yyutbd.1483719
- Thirusendura, S. D., & Saraswathy, S. (2018). Seed viability, seed deterioration and seed quality improvements in stored onion seeds: A review. J. Hortic. Sci. Biotechnol, 93(1), 1–7. https://doi.org/10.1080/14620316.2017.1343103
- Ullah, A., Romdhane, L., Rehman, A., & Farooq, M. (2019). Adequate zinc nutrition improves the tolerance against drought and heat stresses in chickpea. Plant Physiol. Biochem, 143, 11–18. https://doi.org/10.1016/j.plaphy.2019.08.020
- Zhao, A., Yang, S., Wang, B., Tian, X., & Zhang, Y. (2018). Effects of ZnSO4 and Zn-EDTA broadcast or banded to soil on Zn bioavailability in wheat (Triticum aestivum L.) and Zn fractions in soil. Chemosphere, 205, 350–360. https://doi.org/10.1016/ j.chemosphere.2018.04.115
Year 2025,
Volume: 35 Issue: 1, 44 - 52
Abdul Jalil
,
Elkawakib Syam'un
,
Syatrianty Andi Syaiful
Project Number
0667/E5/AL.04/2024
References
- Brar, N. S., Kaushik, P., & Dudi, B. S. (2019). Assessment of natural ageing related physio-biochemical changes in onion seed. Agriculture, 9(163), 2-15.
- Cakmak, I., Brown, P., Colmenero-Flores, J. M., Husted, S., Kutman, B. Y., Nikolic, M., Rengel, Z., Schmidt, S. B., & Zhao, F. J. (2023). Micronutrients. In M. Broadley, P. Brown, I. Cakmak, Z. Rengel, & F. J. Zhao (Eds.), Marschner’s Mineral Nutrition of Plants (pp. 191-248). Elsevier. https://doi.org/10.1016/B978-0-12-819773-8.00017-4
- Chiemento, Jose L. T., Cavali, G. O., de Santos T., & Dornales, A. G. (2020). Quality of tomato seedings produced in substrates. Pesquisa Agropecuaria Gaucha, 26(1), 319-331. https://doi.org/10.36812/pag.2020261319-331
- Choukri, M., Abouabdillah, A. A., Bouabid, R. A., Abd-Elkader, O. H. B., Pacioglu, O. H. C., Boufahja, F. D., & Bourioug, M. (2022). Zn application through seed priming improves productivity and grain nutritional quality of silage corn. Saudi Journal of Biological Sciences, 29(103456), 1-9. https://doi.org/10.1016/j.sjbs.2022.103456
- Demirkaya, M., Dietz, K. J., & Sivritepe, H. O. (2010). Changes in antioxidant enzymes during ageing of onion seeds. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 38(1), 49-52. https://doi.org/10.15835/nbha3814575
- Faried, M., Syam'un, E., & Mantja, K. (2023). Survival rate, disease incidence, and yield of shallots by seed priming and application of tithonia compost enriched with gliocladium virens. Int. J. of Life Sciences and Agriculture Research, 2(5), 57-62. https://doi.org/10.55677/ijlsar/V02I05Y2023-03
- Faried, M., Syam’un, E., Mantja, K. (2024a). Dissimilarities of shallot (Allium ascalonicum L.) seedlings growth and quality through priming with moringa leaf extract. Kufa Journal for Agriculture Science, 16(2), 18-29.
- Faried, M., Syam’un, E., Jalil, A., Cennawati, C., Wijaya, P., & Putri, R. W. (2024b). Can pruning affect the growth of shallot (Allium ascalonicum L.) seedlings from seeds?. Journal of Agricultue Faculty of Ege University, 61(2), 165-174.
- Farooq, M., Romdhane, L., Rehman, A., Al-Alawi, A. K. M., Al-Busaidi, W. M., Asad, S. A., & Lee, D. J. (2020). Integration of seed priming and biochar application improves drought tolerance in cowpea. J. Plant Growth Regul, 1-9. https://doi.org/10.1007/ s00344-020-10245-7.
- Farooq, M. A., Zhang, X., Zafar, M. M., Ma, W., & Zhao, J. (2021). Roles of reactive oxygen species and mitochondria in seed germination. Front. Plant Sci, 12(781734). https://doi.org/10.3389/fpls.2021.781734
- Finch Savage, W. E., & Bassel, G. W. (2016) Seed vigor and crop establishment: Extending performance beyond adaptation. J. Exp. Bot, 67(3), 567–591. https://doi.org/10.1093/jxb/erv490
- Freitas, M. N., Guerra, M. B. B., Adame, A., Moraes, T. F., Junior, J. L., Pérez, C. A., Abdala, D. B., & Cicero, S. M. (2020). A first glance at the micro-ZnO coating of maize (Zea mays L.) seeds: A study of the elemental spatial distribution and Zn speciation analysis. J. Anal. At. Spectrom, 35, 3021–3031. https://doi.org/10.1039/D0JA00282H
- Hassan, U. M., Aamer, M., Chattha, U. M., Haiying, T., Shahzad, B., Barbanti, L., Nawaz, M., Rasheed, A., Afzal, A., Liu, Y., & Guoqin, H. (2020). The critical role of zinc in plants facing the drought stress. Agriculture, 10(9)396, 1-20. https://doi.org/10.3390/agriculture10090396
- Hourston, J. E., Pérez, M., Gawthrop, F., Richards, M., Steinbrecher, T., & Leubner-Metzger, G. (2020). The effects of high oxygen partial pressure on vegetable Allium seeds with a short shelf-life. Planta, 251(105), 1-9. https://doi.org/10.1007/s00425-020-03398-y
- Imran, M., Mahmood, A., Neumann, G., & Boelt, B. (2021). Zinc seed priming improves spinach germination at low temperature. Agriculture, 11(271), 1-12. https://doi.org/10.3390/agriculture11030271
- Kumar, R. J., Pandey, S., & Bose, B. (2021). Seed priming with Mg(NO3)2 and ZnSO4 salts triggers physio-biochemical and antioxidant defense to induce water stress adaptation in wheat (Triticum aestivum L.). Plant Stress, 2(100037), 1-12. https://doi.org/10.1016/j.stress.2021.100037
- Małecka, A., Ciszewska, L., Staszak, A., & Ratajczak, E. (2021). Relationship between mitochondrial changes and seed aging as a limitation of viability for the storage of beech seed (Fagus sylvatica L.). PeerJ, 9(e10569), 1-13. http://doi.org/10.7717/peerj.10569
- Mohammed, W., Woldetsadik, K., & Kabede, B. (2018). Registration of a new improve huruta shallot variety with true seed production potensial. East African Journal of Sciences, 12(1), 77-82.
- Pagano, A., Macovei, A., Xia, X., Padula, G., Hołubowicz, R., Balestrazzi, A. (2023). Seed priming applied to onion-like crops: state of the art and open questions. Agronomy, 13, 288. https://doi.org/10.3390/agronomy13020288
- Pangestuti, R., Sulistiyaningsih, E., Kurniasi, B., & Murti, R. H. (2021). Improving seed germination and seedling growth of true seed shallot (TSS) using plant growth regulator seed priming. In IOP Conference Series: Earth and Environmental Science, 883(012024), 1-8. https://doi.org/10.1088/1755-1315/883/1/012024
- Pehlivan, F. E. (2017). Free radicals and antioxidant system in seed biology. In J.C. Jimenez-Lopez (Ed.), Advances in Seed Biology (pp. 167-183). InTech. http://dx.doi.org/10.5772/intechopen.70837
- Poudel, P., Gioia, D. F., Lambert, J. D., & Connolly, E. L. (2023). Zinc biofortification through seed nutri-priming using alternative zinc sources and concentration levels in pea and sunflower microgreens. Front. Plant Sci, 14(1177844). https://doi.org/10.3389/fpls.2023.1177844
- Rehman, A., Farooq, M., Naveed, M., Nawaz, A., & Shahzad, B. (2018a). Seed priming of Zn with endophytic bacteria improves the productivity and grain biofortification of bread wheat. Eur. J. Agron, 218, 98–107. https://doi.org/10.1016/j.eja.2018.01.017
- Rehman, A., Farooq, M., Naveed, M., Ozturk, L., & Nawaz, A. (2018b). Pseudomonas-aided zinc application improves the productivity and biofortification of bread wheat. Crop Pasture Sci, 69(7), 659–672. https://doi.org/10.1071/CP17441
- Rehman, A., Farooq, M., Ozturk, L., Asif, M., & Siddique, K. H. M. (2018c). Zinc nutrition in wheat-based cropping systems. Plant Soil, 422(1-2), 283–315. https://doi.org/10.1007/s11104-017-3507-3
- Rehman, A., Farooq, M., Asif, M., & Ozturk, L. (2019). Supra-optimal growth temperature exacerbates adverse effects of low Zn supply in wheat. J. Plant Nutr. Soil Sci, 182(4), 656–666. https://doi.org/10.1002/jpln.201800654
- Saranya, N., Renugadevi, J., Raja, K., Rajashree, V., & Hemalatha, G. (2017). Seed priming studies for vigour enhancement in onion CO onion (5). J. Pharm. Phytochem. 6(3), 77–82.
- Schober, P., Boer, C., & Schwarte, L. (2018). Correlation coefficient: Appropriate use and interpretation. Anesthesia and Analgesia, 126(5), 1763-1768. https://doi.org/10.1213/ANE.0000000000002864
- Seddigh, M., Hossein, A., Goftarmanesh, K., & Ghasemi, S. (2016). The effectiveness of seed priming with synthetic zinc-amino acid chelates in comparison with soil-applied ZnSO4 in improving yield and zinc availability of wheat grain. J. Plant Nutr, 39(3), 417–427. https://doi.org/10.1080/01904167.2015.1069340
- Sivritepe, H. O., & Demirkaya, M. (2012). Does humidification technique accomplish physiological enhancement better than priming in onion seeds?. Acta Horticulturae, 960, 237-244. https://doi.org/10.17660/ActaHortic.2012.960.34
- Syam’un, E., Mantja, K., Ulfa, F., Junaid, M., Jayadi, M., Sjam, S., Said, M. I., Suhardi, S., & Syamsia, S. (2024). Influence of variety, beneficial fungi, and application on the growth and production of shallot (Allium ascalonicum L.). Yuzuncu Yil University Journal of Agricultural Sciences, 34(4), 559-570. https://doi.org/10.29133/yyutbd.1483719
- Thirusendura, S. D., & Saraswathy, S. (2018). Seed viability, seed deterioration and seed quality improvements in stored onion seeds: A review. J. Hortic. Sci. Biotechnol, 93(1), 1–7. https://doi.org/10.1080/14620316.2017.1343103
- Ullah, A., Romdhane, L., Rehman, A., & Farooq, M. (2019). Adequate zinc nutrition improves the tolerance against drought and heat stresses in chickpea. Plant Physiol. Biochem, 143, 11–18. https://doi.org/10.1016/j.plaphy.2019.08.020
- Zhao, A., Yang, S., Wang, B., Tian, X., & Zhang, Y. (2018). Effects of ZnSO4 and Zn-EDTA broadcast or banded to soil on Zn bioavailability in wheat (Triticum aestivum L.) and Zn fractions in soil. Chemosphere, 205, 350–360. https://doi.org/10.1016/ j.chemosphere.2018.04.115