Impact of Diet Supplementation with Fenugreek Seeds on Digestibility and Some Rumen and Blood Parameters in Lambs

Year 2025, Volume: 35 Issue: 3, 381 - 391, 30.09.2025

Hoger Hidayet , Kamal Mustafa

https://doi.org/10.29133/yyutbd.1621030

Abstract

This study was designed in two parts to assess the impact of fenugreek seeds (FS) supplementation on lambs' nutrient digestibility and some parameters of rumen and blood. Four lambs were used during the first part of the study by Latin square design, and the lambs were individually fed a concentrate on the level of 3% of live body weight. The first referred to as control (C) had no FS; the second, third, and fourth groups (T1, T2, and T3) were treated by FS at rates of 5,10, and 15%, successively. Each lamb gradually received feed through a preliminary interval for 10 days and subsequent 5 days for sampling, and then every lamb was switched to another treated diet. In the second part of the experiment, in Sacco degradability of the rations and FS was measured by incubating samples in the rumen of two cannulated ram lambs for 1, 2, 4, and 6 hours. The findings show that adding FS did not change the ruminal pH, ammonia-nitrogen concentration (NH3-N), or nutrient digestibility coefficients. Serum glucose (Glu) levels were significantly (P<0.05) reduced by fenugreek seeds, while other blood biochemicals were unaffected. After the feed samples were incubated in the rumen for one hour, there was a notable interaction between time and FS level that increased the Sacco degradability of the FS-treated diets. Six hours post-incubation in the rumen, supplementing FS to the lambs' feed at up to 10% showed a significant elevation in the Sacco DM degradability. In light of the current results, it is concluded that supplementing fenugreek seeds to the lamb’s ration has not influenced nutrient digestibility and ruminal fermentation.

Keywords

digestibility , in Sacco , degradability , Fenugreek

Ethical Statement

Ethical approval for this study was obtained from the University of Duhok Ethics Committee vide number AEC28072023.

Supporting Institution

University of DUHOK

Project Number

2

Thanks

The quality of this work has been enhanced by the authors from the Department of Animal Production at College of Agricultural Engineering Sciences, Department of Pathology and Microbiology at College of Veterinary Medicine, Duhok University.

References

• Abo El-Nor, S. A. H., Khattab, H. M., Kholifi, S. M., El-Sayed, H. M., Abd El-Shaffy, O. H., & Abd El-Rahmam, G. A. (2010). Effect of biological, natural and chemical supplements on the productive performance of lactating buffalos. Revista Veterinaria 21, 623-631. https://2u.pw/b6r7V3M
• Abo-Donia, F., Zaza, G., & Salem, F. (2005). Effect of using natural non-traditional growth promotor: 1-In-vitro evaluation of fenugreek as a natural non-traditional growth promotor and in vivo comparison with monensin in beef cattle rations. Egyptian Journal of Nutrition and Feeds, 8(2),187-205. https://2u.pw/qSIpDNCP
• Abou-Elenin, E. I. M., El-Badawy, M. M., El-Sanafawy, H. A., & Hafez, Y. H. (2016). Effect of using different levels of dry or germinated fenugreek seeds on productive and reproductive performance of dairy Zaraibi goats. Egyptian Journal of Nutrition and Feeds, 19(2), 227-242. Doi: https://doi.org/10.21608/EJNF.2016.74902
• Abu EL-Kassim, M. A., Abd El-Hafez, G. A., Mousa, S. M., & Hassan, E. H. (2018). Effect of dietary onion, garlic and fenugreek seeds powder on feed intake, blood metabolites and rumen fermentation in Ossimi ewes. Assiut Journal of Agricultural Sciences, 49(2), 38-48. Doi: https://doi.org/10.21608/AJAS.2018.9992
• Ahmad, A., Alghamdi, S. S., Mahmood, K. & Afzal, M. (2016). Fenugreek a multipurpose crop: Potentialities and improvements. Saudi Journal of Biological Sciences 23(2), 300–310. Doi: https://doi.org/10.1016/j.sjbs.2015.09.015
• Akbağ, H. I., Savas, A. T., & Yüceer, Y. K. (2022). The effect of fenugreek seed (Trigonella foenum-graecum) supplementation on the performance and milk yield characteristics of dairy goats. Archives Animal Breeding 65(4), 385-395. Doi: https://doi.org/10.5194/aab-65-385-2022.
• Al-Shaikh, M. A., Al-Mufarrej, S. I., & Mogawer, H. H. (1999). Effect of fenugreek seeds (Trigonella foenum-graecum L.) on lactational performance of dairy goat. Journal of Applied Animal Research. 16(2), 177-183. Doi: https://doi.org/10.1080/09712119.1999.9706279
• Al-Sherwany, D. A. O. (2015). Feeding effects of fenugreek seeds on intake, milk yield, chemical composition of milk and some biochemical parameters in Hamdani Ewes. Al-Anbar Journal of Veterinary Sciences, 8(1), 49-54. https://www.iasj.net/iasj/article/107599
• Al-Wazeer, A. A. (2017). Effect of fenugreek seeds supplementation on growth performance, digestion coefficient, rumen fermentation and some blood metabolites of Awassi Lambs. Kufa Journal of Veterinary Medical Sciences. 8(1), 8-18. https://journal.uokufa.edu.iq/index.php/kjvs/article/view/4324/3985
• Broderick, G. A. & Kang, J. H. (1980). Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media. Journal of Dairy Science. 63(1), 64-75. Doi: https://doi.org/10.3168/jds.S0022-0302(80)82888-8
• Çayıroğlu, H., Coşkun, F., Çayan, H., Filik, A. G., & Şahin, A. (2023). Effects of supplemental feeding of fenugreek (Trigonella foenum-graecum L.) seed on milk yield, composition, sensory properties and some blood parameters in lactating goats. Pakistan journal of Zoology. 55(1), 1-9. Doi: https://dx.doi.org/10.17582/journal.pjz/20210817140841
• Ceyhan, M. F., & Erdogan, S. (2025). A study on the rumen fermentation characteristics and rumen ciliates of fattened cattle and sheep slaughtered in Van Province. Yuzuncu Yil University Journal of Agricultural Sciences, 30(1), 354-362. https://doi.org/10.53433/yyufbed.1600490
• Devi, B. A., Kamalakkannan, N., & Prince, P. S. M. (2003). Supplementation of Fenugreek leaves to diabetic rats. Effect on carbohydrate metabolic enzymes in diabetic liver and kidney. Phytotherapy Ressearch, 17(10), 1231-1233. Doi: https://doi.org/10.1002/ptr.1357
• Duncan, C. B. (1955). Multiple range and multiple “F” test. Biometric, 11, 1-12.
• El-Tarabany, A. A., Teama, F. E. I., Atta, M. A. A., & El-Tarabany, M. S. (2018). Impact of dietary fenugreek seeds on lactational performance and blood biochemical and hematological parameters of dairy goats under hot summer conditions. Mljekarstvo, 68(3), 214-223. https://core.ac.uk/download/pdf/212470618.pdf
• Elmnan, A.B., Rewais, F.A., Fadel Elseed, A. M. A, Mahala, A. G., & Amasiab, E. O. (2013). Effect of supplementation of fenugreek (Trigonella foenum-graecum L.) seeds on feed intake, digestibility, n-balance and rumen environment of nubian goats. International Journal of Development and Sustainability, 2(2), 1214-1223. https://isdsnet.com/ijds-v2n2-57.pdf
• Hasin, D., Pampori, Z. A., & Sheikh, A. A., (2019). Milk production and hormonal profile as affected by Fenugreek supplementation in lactating goats of Kashmir valley. Biological Rhythm Research, 52(7), 986-993. Doi: https://doi.org/10.1080/09291016.2019.1608732
• Hidayet, H. M., & Mustafa, K. N. (2020). Effect of feeding oak (quercus aegilops) acorns on nutrient digestibility, nitrogen balance, ruminal fluid characteristics and some blood metabolites in sheep and goats. Journal of Duhok University, Agriculture and Veterinary Sciences, 23(2), 44-54. Doi: https://doi.org/10.26682/ajuod.2020.23.2.7
• Ibidhi, R., & Ben Salem, H. (2022). Effect of daily or intermittent addition of fenugreek (Trigonella foenum graecum L.) seeds to concentrate on intake, digestion, and growth performance of Barbarine lamb. Small Ruminant Research, 216, 1-7. Doi: https://doi.org/10.1016/j.smallrumres.2022.106792
• Kirar, M., Ghosh, S., Baghel, R. P. S., Jain, A., Lakhani, G. P., Roy, B., & Jain, A. K., (2018). Performance of lactating murrah buffaloes supplemented with fenugreek (Methi) seed. International Journal of current Microbiology and Applied Sciences, 7(7), 70-77. Doi: https://doi.org/10.20546/ijcmas.2018.707.009
• Kirar, M., Ghosh, S., Baghel, R. P. S., Jain, A., Lakhani, G. P., & Roy, B. (2020). Effect of Fenugreek (Methi) Seed supplementation on performance of lactating Murrah Buffaloes. Buffalo Bulletin. 39(2), 175-182. https://kuojs.lib.ku.ac.th/index.php/BufBu/article/view/605
• Kobeisy, M., Soliman, I. A., Mohamed, S. F., & Al-Khateeb, A., (2017). Effect of dietary addition of lupin and/or fenugreek seeds on thermo-respiratory response and some blood constituents of Rahmani sheep. Egyptian Journal of Animal Production, 54(3), 185-192. Doi: https://doi.org/10.21608/EJAP.2017.93263
• Komáromyová, M. D., Mravcˇáková, D., Petric, K., Kucková, M., Babják, M. U., Dolinská, A., Königová, M., Mad’arová, E., Pruszyn ́ska-Oszmałek, A., damCieslak, K., Cobanová, Z., Váradyová, & Várady, M. (2021). Effects of Medicinal Plants and Organic Selenium against Ovine Haemonchosis. Animals, 11, 1319. Doi: https://doi.org/10.3390/ani11051319
• Kumar, D., Singh, P., Chaturvedi, V. B., & Verma, A. K. (2016). In Vitro digestibility and rumen fermentation parameters of feeds as affected by supplementary amla powder and Fenugreek seeds. Indian Journal of Animal Nutrition. 33(1), 64-69.10.5958/2231-6744.2016.00011.6
• Maher, A. & Nadya, M. (2013). Effect of using Fenugreek seeds on milk yield and some blood characteristics in local Friesian cows. Tikrit Journal for Agricultural Sciences, 13(2), 70-75. https://2u.pw/fXWUtsCJ
• Makkar, H. P. S., Sen, S., Blummel, M., & Becker, K. (1998). Effects of fractions containing saponins from Yucca schidigera, Quillaja saponaria and Acaciaauriculoformis on rumen fermentation. Journal of Agricultural and Food Chemistry., 46(10), 4324–4328. https://doi.org/10.1021/jf980269q
• Mishkinsky, J. S., Goldschmied, A., Joseph, B., Aharonson, Z., & Sulman, F. G. (1974). Hypoglycemic effect of Trigonella foenum graecum and Lupinus termis (leguminosae) seeds and their major alkaloids in alloxan-diabetic and normal rats. Archives Internationales de Pharmacodynamie et de Therapi, 210(1), 27-37. https://pubmed.ncbi.nlm.nih.gov/4280278/
• Mustafa, K. N. S., & Yateem, Ch. A. M. (2019). Effect of supplementation different levels of fenugreek seeds (trigonella foenum graceum) on milk yield, composition and some blood parameters in Meriz goats. International Journal of Advances in Science, Engineering and Technology, 7(4), 66-71. https://www.iraj.in/journal/journal_file/journal_pdf/6-600-157865555166-71.pdf
• Mustafa, K. N., Hidayet, H. M., & Yateem, C. A. (2022). Effect of supplementation of nigella sativa oil on nutrient digestibility, some blood metabolites and rumen parameters in Karadi Lambs. Yuzuncu Yil University Journal of Agricultural Sciences, 32(3), 584-590. Doi: https://doi.org/10.29133/yyutbd.1104782
• Newbold, C. J., de la Fuente, G., Belanche, A., Ramos-Morales, E., & McEwan, N. R. (2015). The Role of Ciliate Protozoa in the Rumen. Frontiers in Microbiology, 6, 1-14. https://www.frontiersin.org/articles/10.3389/fmicb.2015.01313/full
• Newbold, C. J., El Hassan S. M. E., Wang, J., Ortega, M. E., & Wallace R. J. (1997). Influence of foliage from African multipurpose tree on activity of rumen protozoa and bacteria. British Journal of Nutrition, 78, 237 – 249. https://2u.pw/8oZ2ZHj
• Örün, M., & Erdoğan, S. (2022). Determination of in vitro true digestibility and relative feed values of alternative roughage sources. Yuzuncu Yil University Journal of Agricultural Sciences, 32(3), 576-583. Doi: https://doi.org/10.29133/yyutbd.1103508
• Paine, C. A., Crawshaw, R., & Barber, W. P. (1982). A complete exchange method for the in sacco estimation of rumen degradability on a routine basis. In Forage protein in ruminant animal production. Occasional publication No. 6. British Society of Animal Production. D.G. Thompson, D.E. Beever and R.G. Gunn. Doi: https://doi.org/10.1017/S0263967X00030615
• Raju, J., Gupta, D., Rao, A. R., Yadava, P. K., & Baquer, N. Z., (2001). Trigonella foenum graecum (Fenugreek) seed powder improves glucose homeostasis in alloxan diabetic rat tissues by reversing the altered glycolytic, gluconeogenic and lipogenic enzymes. Molecular and Cellular Biochemistry, 224, 45-51. 10.1023/a:1011974630828
• Rao, P. U., & Sharma, R. D., (1987). An evaluation of protein quality of Fenugreek seeds (Trigonella foenum-graecum) and their supplementary effects. Food Chemistry, 24(1), 1-9. https://www.sciencedirect.com/science/article/abs/pii/0308814687900781?via%3Dihub
• Saleh, Sh. A. W., & Alssadi, S. A. A. (2022). Study effects of aqueous extract of local fenugreek seeds and olive leaves in some biochemical traits at Iraqi Awassi sheep. Journal of Pharmaceutical Negative Results, 13(Special Issue, 8),250-255. https://doi.org/10.47750/pnr.2022.13.S08.038
• Salem, F., Abu, E.-E., Marghany, M., & El-Amary, H. (2004). Effect of medical herbs and plants as feed additives on sheep performance. Journal of Animal and Poultry Production, 29, 2303-2320. https://journals.ekb.eg/article_239162_604c3bdffff40849a1226ec6057ba892.pdf
• Santoso, B., Kilmaskossub A., & Sambodo, P. (2007a). Effects of saponins from Biophytum petersianum Klotzsch on ruminal fermentation, microbial protein synthesis and nitrogen utilization in goats. Animal Feed Science and Technology, 137(1-2), 58–68. https://2u.pw/z3KOQMM
• Santoso, B., & dan Umiyati, M. N. L. (2007b). Chemical composition and nutrient degradation of elephant grass silage ensiled with black tea waste. Animal Production, 9(3), 160-165. (In Indonesian). file:///Users/kamal/Downloads/172-637-1-PB.pdf
• SAS (2001). SAS system under P. C. Dos. SAS institute. Ine. Cary. NC.
• Sen, S., Makkar, H. P. S., Muetzel S., & Becker K. (1998). Effect of Quillaja saponaria saponins and Yucca schidigera plant extract on growth of Escherichia coli. Letters in Applied Microbiology, 27(1), 35–38 https://www.researchgate.net/publication/13562546
• Smit, H. P. J. (2014). The effect of a natural feed additive, fenugreek, on feed digestibility and milk response in dairy goats. M.Sc. Thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Animal Science. Faculty of AgriSciences at Stellenbosch University. https://scholar.sun.ac.za/items/496c474a-1777-492a-a628-65a5604b9504
• Thiex, N., Novotny, L., & Crawford, A. (2012). Determination of ash in animal feed: AOAC official method 942.05 revisited. Journal of AOAC International, 95, 1392-1397.
• Wallace R. J., & McPherson, C. A. (1987). Factors affecting the rate of breakdown of bacterial protein in rumen fluid. British Journal of Nutrition, 58(2), 313–323. https://pubmed.ncbi.nlm.nih.gov/3118940/
• Wani, S. A., & Kumar, P. (2018). Fenugreek: A review on its nutraceutical properties and utilization in various food products. Journal of the Saudi Society of Agricultural Sciences, 17(2), 97-106. http://dx.doi.org/10.1016/j.jssas.2016.01.007
• Wang, Y, McAllister, T. A., & Yanke, L. J. (2000). Effect of steroidal saponin from Yucca schidigera extract on ruminal microbes. Journal of Applied Microbiology, 88(5), 887–896. Doi: 10.1046/j.1365-2672.2000.01054.x
• Williams, A. G., & Coleman, G. S. (1997). The rumen protozoa. In The Rumen Microbial Ecosystem, pp. 72 – 120[PN Hobson and CS Stewart, editors]. London: Blackie Academic and Professional.
• Yetem, C. A. (2015). Effect of supplement different levels of fenugreek seeds (Trigonella foenum graceum) on milk yield, composition and some blood parameters Meriz goats. MSc. Thesis. Animal production. University of Duhok.