Subklinik Mastitisli İneklerden İzole Edilen Stafilokok Suşlarının Bazı Virulens Faktörleri ve Antibiyotik Direnci

Year 2011, Volume: 22 Issue: 1, 17 - 21, 01.03.2011

Osman Yaşar Tel Oktay Keskin

Abstract

Keywords

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Subklinik Mastitisli İneklerden İzole Edilen Stafilokok Suşlarının Bazı Virulens Faktörleri ve Antibiyotik Direnci

Abstract

Bu çalışmada subklinik mastitislerden izole edilen 108 adet koagulaz pozitif stafilokok (KPS) ve 50 adet koagulaz negatif stafilokok (KNS) olmak üzere toplam 158 suşun bazı virulens faktörleri [deoksiribonukleaz (DNaz), termonukleaz (TNaz), kapsül, biyofilm üretimi, hemoliz, protein A özellikleri] ve antibiyotiklere dirençleri araştırıldı. Çalışmada, DNaz, TNaz, kapsül, biofilm, hemoliz sonuçları sırasıyla, KPS izolatlarında 98 (%90), 72 (%66), 62 (%57), 76 (%70), 84 (%77) adedi, KNS izolatlarında ise 4 (%8), 11 (%22), 4 (%8), 26 (%52), 21 (%42) adedi pozitif olarak bulundu. Ayrıca KPS suşlarının 98 (%90)’inde Protein A saptandı. İzole edilen Stafilokokların antibiyotiklere duyarlılıkları Kirby Bauer Disk Difüzyon Yöntemi’ne göre yapıldı. 108 adet KPS suşunun yapılan antibiyogramı sonucunda, 98’i (%90.7) penicillin, 92’si (%85) ampicillin, 30’u (%27.7) norfloxacin, 20’si (%18.5) amoxicillin-clavulanic acid, 13’ü (%12) gentamicin, 12’si (%11) ciprofloxacin, 8’i (%7.4) sulphamethoxazole-trimethoprim, 6’sı (%5.5) oxacillin ve erythromycin, 4’ü (%3.7) tetracycline’e dirençli olarak bulunurken bütün suşlar clindamycin, cefuroxime ve vancomycin’e duyarlı olarak saptandı. KNS suşlarının ise 38’i (%76) penicillin, 30’u (%60) ampicillin, 18’i (%36) erythromycin, 10’u (%20) gentamicin ve tetracycline’e 9’u ( %18) amoxicillin-clavulanic acid, 8’i (%16) ciprofloxacin, 5’i (%10) norfloxacin ve 2’si (%4) clindamycin’e dirençli bulunurken oxacillin, cefuroxime, vancomycin ve sulphamethoxazole-trimethoprim’e duyarlı olduğu saptandı. Ayrıca, KPS’lerde saptanan metisilin direnci PCR yöntemiyle doğrulandı. Sonuç olarak, KPS’lerde KNS’lere göre virulens faktörlerinin yüksek olduğu, bu faktörlerin patojenitede önemli rol oynadığı ve etkenlerin genel olarak penicillin ve ampicillin dışındaki antibiyotiklere duyarlı olduğu sonucuna varıldı

Keywords

Koagulaz Pozitif Stafilokok, Koagulaz Negatif Stafilokok, Virulens Faktörleri, Antibiyotik Direnci

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