Effects of Combination of Asetylmethionine, L-Carnitine, Vitamin E and Vitamin B12 on Some Clinical, Haematological and Biochemical Parameters in Cattle

Year 2008, Volume: 19 Issue: 1, 9 - 14, 01.03.2008

Handan Hilal Arslan Cevat Nisbet Devrim Sarıpınar Sena Çenesiz Metin Çenesiz

Abstract

In this study, evaluation of the effects of asetylmethionine, l-carnitine, vitamin E and vitamin B12 combination on some clinical, haematological and biochemical parameters in cattle was aimed. Thirty two animals, which were affected by various stressors, clinically weakness body condition, dullness, drooping, anorexic and apathetic, were used in the study. A commercial product which contained asetylmethionine, l-carnitine, vitamin E and vitamin B12 was administrated 5 ml/100 kg b.w. once a day for 7 days via intramuscularly. General clinical examinations were made in the all animals before and after the drug applications and disorders of general condition and feather, apathy/signs of depression, appetite were scored (0=absent, 4=very severe). Also, blood samples were collected into empty and anticoagulant contained tubes for haematological and biochemical evaluations before and after the drug applications. Differential leukocyte and serum ALT, AST, total protein and total cholesterol levels for liver functions were determined. In addition, serum malondialdehit (MDA) and seruloplasmin levels were determined to evaluate antioxidant metabolism. After the study, microhaematocrit, red blood cell, white blood cells counts and total protein levels before and after treatment were found to be significant (p<0.05). But other haematological and biochemical parameters were not found statistically different (p>0.05). In the study, mean clinical score was determined 2.44±0.13 before the treatment and 0.26±0.07 after the treatment. In the evaluation of clinical examinations before and after the treatment, investigated clinical scores of 28 animals (87.5%) were found 3 and 4 before the treatment and less than 1 after the treatment. Satisfactory clinical recovery was considered in these animals. 

Keywords

Acetylmethionine, Biochemical parameters, Cattle, Hematology, L-carnitine, Vitamin E, Vitamin B12

Sığırlarda Asetilmetiyonin, L-Karnitin, Vitamin E ve Vitamin B12 Kombinasyonunun Bazı Klinik, Hematolojik ve Biyokimyasal Parametreler Üzerine Etkisi

Abstract

Bu çalışmada asetilmetiyonin, l-karnitin, vitamin E ve vitamin B12 kombinasyonunun sığırlarda bazı klinik, hematolojk ve biyokimyasal parametreler üzerine etkisinin değerlendirilmesi amaçlandı. Çalışmada, çeşitli stres faktörlerine maruz kalmış, klinik olarak genel vücut kondisyonu iyi olmayan, durgun, halsiz, iştahsız ve çevreye ilgisi azalmış 32 adet sığır kullanıldı. Hayvanlara asetilmetiyonin, l-karnitin, vitamin E ve vitamin B12 içeren bir ticari ürün (Metabolase Forte, FATRO-VETAŞ) 100 kg canlı ağırlığa 5 ml dozda, kas içi yolla günde bir defa olmak üzere 7 gün süre ile uygulandı. Hayvanların ilaç uygulanmasından önce ve sonra klinik muayenesi yapılarak, genel durum bozukluğu, kıl örtüsünde düzensizlik, çevreye ilgisizlik/depresyon, iştah durumları skorlanarak (0=yok, 4=çok şiddetli) değerlendirildi. Yine ilaç uygulaması öncesi ve sonrasında antikoagülanlı ve boş tüplere hematolojik ve biyokimyasal değerlendirmeler amacıyla kan alındı. Formül lökosit tablosu ile karaciğer fonksiyonları için serum ALT, AST, total protein ve kolesterol değerleri belirlendi. Ayrıca hayvanlardaki antioksidan metabolizma hakkında değerlendirme yapmak amacıyla serum MDA ve seruloplazmin düzeyleri tespit edildi. Çalışma sonunda hematokrit değer, alyuvar ve akyuvar sayıları ile total protein seviyesinde ilaç öncesi ve sonrasında istatistiksel olarak fark tespit edilirken (p 0.05). Çalışmada, tedavi öncesi 2.44±0.13 olan ortalama klinik skor tedavi sonrası 0.26±0.07 olarak bulundu. Tedavi öncesi ve sonrası yapılan klinik değerlendirmelerde hayvanların 28 adedinde (%87.5) başlangıçta incelenen parametreler yönünden 3 ve 4 olan klinik skorların tedavi sonrası 1’in altına düştüğü belirlendi. Bu hayvanların klinik olarak iyi derecede iyileştikleri kabul edildi

Keywords

Asetilmetiyonin, Biyokimyasal parametreler, Hematoloji, L-karnitin, Sığır, Vitamin E, Vitamin B12

References

• Aouffen M, Paquin J, Furtos A, Waldron KC, Mateescu MA (2004): Oxidative aggregation of ceruloplasmin induced by hydrogen peroxide is prevented by pyruvate. Free Radical Res. 38: 19-26.
• Arthington JD, Eicher SD, Kunkle WE (2003): Effect of transportation and commingling on the acute-phase protein response, growth, and feed intake of newly weaned beef calves. J Anim. Sci. 81: 1120-1125.
• Batmaz H., Aytug N., Kennerman E (1998): Klinik tanıda laboratuar bulgularının değerlendirilmesi. Uludağ
• Üniversitesi Veteriner Fakültesi Kurs Kitabı, Bursa. Brzezinska-Slebodzinska E, Miller JK, Quigley JD, Moore R (1994): Antioxidant status of dairy cows supplemented prepartum with vitamin e and selenium. J. Dairy. Sci. 77: 3087-3095.
• Canoruç N, Canoruç F, Aslan Ç, Yılmaz Ş, Turgut C, Dursun M, Akkuş Z, Kale E (2006): Karaciğer hastalıklarında (siroz veya hepatit) homosistein ve selenyum düzeyleri. Akademik Gastroenteroloji Dergisi. 5: 30.
• Chirase NK, Greene LW, Purdy CW, Loan RW, Auvermann BW, Parker DB, Walborg EF Jr, Stevenson DE, Xu Y, Klaunig JE (2004): Effect of transport stress on respiratory disease, serum antioxidant status, and serum concentrations of lipid peroxidation biomarkers in beef cattle. Am. J. Vet. Res. 65: 860-864.
• Clombo JP, Richterich R (1964): Zur bestimmung des ceruloplasmin im plasma. Schweiz Med. Wschr. 94: 715
• Conner JG, Eckersall PD, Wiseman A, Bain RK, Douglas TA (1989): Acute phase response in calves following infection with pasteurella haemolytica, ostertagia ostertagi and endotoxin administration. Res. Vet. Sci. 47: 207.
• Edmonson AJ, Lean I, Weaver LD, Farver T, Webster G (1989): A body condition scoring chart for holstein dairy cows. J. Dairy. Sci. 72:68-78.
• Erkin B, Dokmeci D, Altaner S, Turan FN (2006):
• Gastroprotective effect of l-carnitine on indomethacin- induced gastric mucosal injury in rats: a preliminary study. Folia Med (Plovdiv). 48: 86-89. Feldman BF, Zikl JG, Jain NC (2000): Schalm’s Veterinary
• Hematology. 5th ed. Lippincott Williams& Wilkins, Philadelphia. Girard CL, Matte JJ (2005): Effects of intramuscular injections of vitamin b12 on lactation performance of dairy cows fed dietary supplements of folic acid and rumen-protected methionine. J. Dairy Sci. 88: 671–676.
• Gruys E, Oblowo, MJ, Toussaint JM (1994): Diagnosis significanse of major acute phase proteins in veterinary clinical chemistry: a review. B. Vet. I. Pulawy. 64: 1009-1015.
• Gupta S, Gupta HK, Soni J (2004): Effect of vitamin e and selenium supplementation on concentrations of plasma cortisol and erythrocyte lipid peroxides and the incidence of retained fetal membranes in crossbred dairy cattle. Theriogenology. 64: 1273–
• Halliwell B (1996): Antioxidants in human health and disease. Annu. Rev. Nutr. 16: 33-50.
• Halliwell B, ve Gutteridge JM (1984): Free radicals, lipid peroxidation and cell damage. Lancet. 2: 1095.
• İzgüt-Uysal VN, Ağaç A, Derin N (2001): Effect of carnitine on stress-induced lipid peroxidation in rat gastric mucosa. J Gastroenterol. 36: 231–236.
• Karapehlivan M, Uzlu E, Kaya N, Kankavi O, Ural K, Çitil M (2007): Investigation of Some Biochemical Parameters and the Antioxidant System in Calves with Dermatophytosis. Turk. J. Vet. Anim. Sci. 31(2): 85-89
• Kleczkowski M, Klucinski W, Sikora J, Zdanowicz M, Dziekan P (2003): Role of antioxidants in the protection against oxidative stress in cattle nonenzymatic mechanism. Pol J. Vet. Sci. : 301-308.
• Koeck T., Kremser K (2003): L-carnitine alters nitric oxide synthase activity in fibroblasts depending on the peroxisomal status.
• Int. J. Biochem. Cell. Biol. 35:149–156. Konuk T (1981): Pratik Fizyoloji I. 2. baskı. Ankara Üniv. Basımevi, Ankara.
• Mercan U (2004): Toksikolojide serbest radikallerin önemi. YYU Vet. Fak. Derg. 15: 91-96.
• Napoli C, Ignarro LJ (2001): Nitric oxide and atherosclerosis.
• Nitric. Oxide-Biol. Ch. 5: 88-97. Nunes VA, Gozzo AJ, Cruz-Silva I, Juliano MA, Viel TA, Godinho RO, Meirelles FV, Sampaio MU, Sampaio CA, Araujo MS (2005): Vitamin E prevents cell death induced by mild oxidative stress in chicken skeletal muscle cells. Comp. Biochem. Phys. C. 141: 225-240.
• Ortigues-Marty I, Micol D, Prache S, Dozias D, Girard Cl (2005): Nutritional value of meat: the influence of nutrition and physical activity on vitamin B12 concentrations in ruminant tissues. Reprod. Nutr. Dev. 45: –467.
• Oz HS, Chen TS, Neuman M (2007): Methionine deficiency and hepatic injury in a dietary steatohepatitis model. Cell. Mol. Biol. (Noisy-le-grand). 53: 1-2.
• Rao CR (1973): Linear statistical inference and its applications. John&Sons, New York.
• Reaven PD, Napoli C, Merat S, Witztum JL (1999):
• Lipoprotein modification and atherosclerosis in aging. Exp. Gerentol. 34: 527-537. Rezaei AS, Dalir-Naghadeh B (2006): Evaluation of antioxidant status and oxidative stress in cattle naturally infected with Theileria annulata. Vet. Parasitol. 142: 179
• Rulquin H, Delaby L (1997): Effects of the energy balance of dairy cows on lactational responses to rumen- protected methionine. J. Dairy. Sci. 80: 2513-2522.
• Saco Y, Fina M, Gimenez M, Pato R, Piedrafita J, Bassols A (2007): Evaluation of serum cortisol, metabolic parameters, acute phase proteins and faecal corticosterone as indicators of stress in cows. The Veterinary Journal, (basımda).
• Sani M, Ghanem-Boughanmi N, Gadacha W, Sebai H, Boughattas NA, Malondialdehyde content and circadian variations in brain, kidney, liver, and plasma of mice. Chronobiol. Int. 24: 671-685. Selvaraj N, Bobby Z, Sridhar MG (2007): Oxidative stress:
• Does it play a role in the genesis of early glycated proteins? Medical Hypotheses (basımda). Sheridan JF, Dobbs C, Brown D, Zwilling B (1994):
• Psychoneuroimmunology: Stress effects on pathogenesis and immunity during infection. Clin. Microbiol. Rev. 7: 200-212. Vangroenweghe F, Duchateau L, Boutet P, Lekeux P, Rainard P, Paape MJ, Burvenich C. (2005): Effect of carprofen treatment following experimentally ınduced Escherichia coli mastitis in primiparous. Cows J. Dairy Sci. 88:2361–2376
• Wuerges J, Garau G, Geremia S, Fedosov SN, Petersen TE, Randaccio L (2006): Structural basis for mammalian vitamin B12 transport by transcobalamin. Proc. Natl. Acad. Sci .U S A. 103: 4391.
• Yoshioka T, Kawada K, Shimada T, Mori M (1979): Lipid peroxidation in maternal and cord blood and protective mechanism aganist actived oxygen toxicity in the blood. Am. J. Obstet. Gynecol. : 372–376.