Biotechnologically Relevant Filamentous Fungi Obtained by the System ‘Clustered Regularly Interspaced Short Palindromic Repeats and Associated Proteins'

Year 2020, Volume: 1 Issue: 2, 1 - 8, 22.04.2020

Evrim Özkale

Abstract

Several strains of filamentous fungi have been used to produce wide spectrum of natural products including organic acids, antibiotics, other useful proteins for centuries. Besides of these innate features, due to having versatile abilities, many filamentous fungi can be used int he field of bioproduction as host microorganisms.
Despite the relevant importance and dense use of filamentous fungi in biotechnology, detailed knowledge about the molecular biology of the metabolism is not available most of them except a few model fungi. Targeting biosytnhetic genes to edit can be achieved existing tools, however, these genetic tools are inefficient and difficult to employ in wide array of filamentous fungi by the reasons such as the low editing efficiency and the therefore large amount of labor time. Recently, CRISPR/Cas9 has become growing gene-editing technology due to significant advantages over existing editing tools such as high efficiency, easy operation, the possibility of multigene editing This technology has been started to introduce to various species of filamentous fungi since 2015. The loss or gain of function of such mutant alleles is the major application of CRIPSR-Cas mediated genome engineering.
In this review, state-on-art applications of the CRISPR/Cas9 technology in several filamentous fungi were summarized and the further prospects of this technology briefly discussed.

Keywords

filamentous fungi, genome editing, CRISPR/Cas9 technology, , strain improvement

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