Molecular Characterization of Salmonella typhimurium from meat samples in Multan, Pakistan

Year 2020, Volume: 1 Issue: 2, 35 - 41, 22.04.2020

Sadaf Parvez Muhammad Safdar , Yasmeen Junejo Sana Zaheer

Abstract

Global meat production has increased by 20% in the past 10 years only and has tripled over in the last 4 years. Most of the systemic intestinal infections are caused by gram-negative bacteria in humans, called Salmonella typhimurium. This research aimed to identify Salmonella typhimurium at molecular level through optimized PCR. The work was done by collecting samples from five different regions of Multan, Pakistan. The bacterial cells were grown on petri dishes and subsequently on broth media. DNA was extracted following protocol specified for bacterial cells. The quality of DNA was checked by agarose gel electrophoresis in 0.8% agarose gel for 45 minutes. The PCR was optimized by amplifying the DNA with species-specific primers. Further sensitivity of PCR was done by evaluating the identified DNA of Salmonella typhimurium by diluting the DNA up to 0.01 ng. 10% of the total collected samples for the identification of Salmonella typhimurium by PCR from the raw meat samples. This study revealed that raw meat was contaminated with Salmonella typhimurium at different meat retail shops in Multan. This study may help with the management of Salmonella typhimurium in Pakistan.

Keywords

Salmonella typhimurium, Meat samples, Bacterial culture, DNA extraction, Molecular Characterization

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