Molecular identification of Argulus japonicus in red cap oranda goldfish (Carassius auratus) in Multan, Pakistan

Year 2020, Volume: 1 Issue: 2, 42 - 46, 22.04.2020

Warda Khan Muhammad Safdar Yasmeen Junejo

Abstract

Fish aquariums provide the foremost characteristics of morphology and identification of the genus Argulus (Crustacea: Branchiura). Member of genus Argulus, or fish louse are ectoparasites of fresh-water fishes. The present study was investigated the molecular analysis of Argulus japonicus. The work was done by collecting samples from three different regions of Multan, Pakistan. DNA was extracted following protocol specified for for the isolation of DNA. The quality of DNA was checked by agarose gel electrophoresis in 0.8% agarose gel for 45 minutes. The PCR was optimized by amplifying the DNA with species-specific primers. Further sensitivity of PCR was done by evaluating the identified DNA of Argulus japonicus by diluting the DNA up to 0.01 ng. The rate of prevalence of Argulus japonicus at Bosan Road was 10%, Chungi no. 09 was 62% and at Dera Adda 76%. This study revealed that Argulus japonicus is prevelented in the gold fish in Multan region. This study may help with the management of Argulus japonicus parasite in fish aquarium at Multan, Pakistan.

Keywords

Argulus japonicus, Redcap oranda goldfish, Molecular identification

References

• Avenant-Oldewage, A. , & Everts, L (2010). Argulus japonicus: Sperm transfer by means of a spermatophore on Carassius auratus (L). Experimental parasitology, 126(2), 232-238.
• Baird, W (1850). The natural history of the British Entomostraca (Vol. 2): Ray society.
• Feroz Khan, K. , Sanker, G. , & Prasanna Kumar, C (2016). Linking eggs and adults of Argulus spp. using mitochondrial DNA barcodes. Mitochondrial DNA Part A, 27(6), 3927-3931.
• Iqbal, Z. , Mumtaz, R. , & Sajjad, R (2013). Argulosis, in some ornamental fishes imported to Lahore, Pakistan. Eur J Vet Med, 2(3), 171-178.
• Jafri, S. , & Mahar, M (2009). New record of two copepod parasites from fresh water fishes of Sindh, Pakistan. Sindh University Research Journal-SURJ (Science Series), 41(1).
• Nowak, B. F (2007). Parasitic diseases in marine cage culture–an example of experimental evolution of parasites? International Journal for Parasitology, 37(6), 581-588.
• Rushton-Mellor, S (1992). Discovery of the fish louse, Argulus japonicus Thiele (Crustacea: Branchiura), in Britain. Aquaculture and Fisheries Management, 23(2), 269-271.
• Tokşen, E (2006). Argulus foliacesus (Crustacea: Branchiura) Infestation on Oscar, Astronotus ocellatus (Cuvier, 1829) and Its Treatment. Su Ürünleri Dergisi, 23(1).
• Walker, P. D. , Flik, G. , & Bonga, S. E. W (2004). The biology of parasites from the genus Argulus and a review of the interactions with its host. In Host-parasite interactions (pp. 107-128): Taylor & Francis.
• Williams, C (1997). Investigation of Diflubenzuron in The Control of Argulus foliaceus L. Carp, Cyprinus carpio L. in Relation to Management Strategies. MSc Thesis University of Plymouth, 143-145.
• Woolhouse, M. , & Webster, J (2000). In search of the Red Queen. Parasitology Today, 16(12), 506-508.
• Yıldız, K. , & Kumantas, A (2002). Argulus foliaceus infection in a goldfish (Carassius auratus). Isr J Vet Med, 57(3), 118-120.