Abstract
In this study, 96 genotypes in the National Ex-situ Olive Germplasm Bank have been characterized by DNAbased marker techniques such as RAPD, AFLP and SSR. In the marker analyses, 215 polymorphic bands from 52
primers in RAPD, 919 polymorphic bands from 26 primers in AFLP and 62 polymorphic bands from 14 primers
in SSR have been obtained. A dendrogram and a genetic distance matrix have been established with the data of
each technique seperately. Besides, the dendrogram and the genetic distance matrix have also been constructed by
evaluating totaly 1196 polymorphic bands as a result of combining the data of these techniques. In the studied 96
genotypes, it has been determined that the lowest genetic distance value was 0.05 and the highest genetic distance
value was 0.84 in RAPD marker analysis. In AFLP marker analysis, the lowest value was 0.15 and the highest
value was 0.71 within the population in the context of genetic distance. As for SSR, the lowest and highest
genetic distance values have been determined as 0.00 and 0.87 respectively. Evaluating the data of RAPD, AFLP
and SSR marker analyses together, it has been determined that the lowest genetic matrix value was 0.14 and the
highest genetic matrix value was 0.70.
References
- Bartolini, G., Prevost, G., Messeri, C., Carignani, G., Menini, UG., 1998, Olive germplasm. Cultivars and World-Wide collections. FAO, Rome, Italy. Belaj A., Satovic Z., Ismaeli H., Panajoti D., Rallo L. and Trujillo I. 2003a, RAPD genetic diversity of Albanian olive germplasm and its relationships with other Mediterranean countries. Euphytica 130: 387-395. Belaj A, Satovic Z, Cipriani G, Baldoni L, Testolin R, Rallo L, Trujillo I, 2003b, Comparative study of the discriminating capacity of RAPD, AFLP and SSR markers and of their effectiveness in establishing genetic relationships in olive, Theor Appl Genet 107:736-744. Botstein, D., White, R.L., Skolnick, M., Davis, R.W., 1980, Construction of a genetic map in man using restriction fragment length polymorphisms. Am J Hum Gen. 32:314–331. Cantini, C., Cimato, A., Sani, G., 1999, Morphological evaluation of olive germplasm present in Tuscany region. Euphytica, 109: 173-181. Carriero, F., Fontanazza, G., Cellini, F. and Giorio G., 2002, Identification of simple sequence repeats(SSRs) in olive (Olea europaea L.).Theor. Appl. Genet. 104: 301–307. Cordeiro, A.I.; Sanchez-Sevilla, J.F.; Alvarez-Tinaut, M.C. & Gomez-Jimenez, M.C., 2008, Genetic diversity assessment in Portugal accessions of Olea europaea by RAPD markers. Biologia Plantarum, 52, (4), 642-647. Doyle, J.J., Doyle, J.L., 1990, Isolation of plant DNA from fresh tissue. Focus, 12. Felsenstein J., 2007, PHYLIP (Phylogeny Inference Package) Version 3.67. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle. http://evolution.gs.washington.edu/phylip.html. Grati-Kamoun, N., Mahmoud, F., Rebai, A. and Gargouri, A., 2006, Genetic diversity of Tunisian olive tree (Olea europaea L.) cultivars assessed by AFLP markers. Genet. Resour. Crop Evol. 53: 265-275. Gülşen, O. ve Mutlu, N., 2005, Bitki biliminde kullanılan genetik markırlar ve kullanım alanları. Alatarım 4(2):27-37. La Mantia, M., Lain, O., Caruso, T., Testolin, R., 2005, SSR based DNA fingerprints reveal the genetic diversity of Sicilian olive (Olea europaea L.) germplasm. J. Hort. Sci. Biot. 80:628-632. Muzzalupo, I., Lombardo, N., Musacchio, A., Noce, M.E., Pellegrino, G., Perri, E. and Sajjad, A., 2006, DNA sequence analysis of microsatellite markers enhances their efficiency for germplasm management in an Italian olive collection, J Am Soc Hortic Sci 131(3):352-359. Muzzalupo, I., Stefanizzi, F., Perri, E., 2009, Evaluation of olives cultivated in southern Italy by simple sequence repeat markers, HortScience 44(3):582-588. Nei, M., 1978, Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics, 89, 583–590. Öztürk, F., 2006, Türkiye’de ve Dünya’da zeytincilik sektörünün genel görünümü. TAYEK, Ege tarımsal araştırma enstitüsü müdürlüğü, yayın no: 125, 45-62. Rallo, L., Barranco, D. and Escobar, F., 1997, El cultivo del olivo. Ediciones mundi prensa. pp. 701. Rekik, I., Salimonti, A., Grati-Kamoun, N., Muzzalupo, I., Perri, E., Rebai, A., 2008, Characterisation and identification of Tunisian olive tree varieties by microsatellite markers. HortScience 43:1371-1376. Remesal-Rodríguez, J., 1996, Economía oleícola: En la antigüedad, p. 47–58. In: consejo oleicola internacional (Ed.), ‘Enciclopedia mundial del olivo, plaza & Janes Editores S.A., Barcelona, Spain. SAS, 1995, SAS/STAT user’s guide. Version 6.12. SAS Institute, Cary, North Carolina. Sefc KM, Lopes MS, Mendonca D, Dos Santos MR., 2000, Identification of microsatellite loci in olive (Olea europaea) and their characaterization in Italian and Iberian olive trees. Mol. Ecol. 9: 1171-1173. Shahriari, M., Omrani, A., Falahati-Anbaran, A., Ghareyazei, B. & Nankali, A., 2008, Identification of Iranian olive cultivars by using RAPD and microsatellite markers. Acta Hort. 791:109-115. Vergarı G., Patumı M., Fontanazza G., 1996, Use of RAPDs markers in the characterisation of olive germplasm. Olivae, 60: 19-22. Yıldırım, A., Kandemir, N., 2001, Bitki biyoteknolojisi-II genetik mühendisliği ve uygulamaları, genetik markörler ve analiz metodları. Selçuk Üniversitesi Basımevi s:334- 363. Zamora, R., Alaiz, M., Hidalgo, F. J., 2001, Influence of cultivar and fruit ripening on olive (Olea europaea) fruit protein content, composition, and antioxidant activity. J. Agric. Food Chem, 49 (9), 4267-4270. Zohary, D., Spiegel-Roy, P., 1975, Beginning of fruit growing in the old world. Science, 187: 319-327. Zohary, D., Hopf, M., 1993, Domestication of plants in the old world. Oxford clarendon press, 137-143.
Abstract
Bu çalışmada ulusal zeytin arazi gen bankasındaki 96 genotip DNA’ya dayalı yöntemler olan RAPD, AFLP ve
SSR markör teknikleri uygulanarak moleküler düzeyde tanımlanmıştır. RAPD markör analizinde 52 primerden
215 polimorfik bant, AFLP markör analizinde 26 primerden 919 polimorfik bant ve SSR markör analizinde ise 14
primerden 62 polimorfik bant elde edilmiştir. Her teknikten elde edilen veriler ile genetik uzaklık matrisi ve
dendrogram oluşturulmuştur. Ayrıca üç tekniğin verilerinin birleştirilmesi sonucunda toplam 1196 adet
polimorfik bant değerlendirilerek dendrogram ve genetik uzaklık matrisi elde edilmiştir. İncelenen 96 genotipte
RAPD markör analizinde en düşük genetik uzaklık değeri 0.05, en yüksek genetik uzaklık değeri 0.84 olarak
tesbit edilmiştir. AFLP markör analizinde popülasyonda genetik uzaklık bakımından en düşük değer 0.15, en
yüksek değer 0.71’dir. SSR markör analizinde ise en düşük ve en yüksek genetik uzaklık değerleri ise 0.00 ile
0.87 olarak saptanmıştır. RAPD, AFLP ve SSR markör analizi verileri birlikte değerlendirilerek elde edilen
genetik uzaklık matrisinde, en düşük değer 0.14, en yüksek değer ise 0.70 olarak belirlenmiştir.
References
- Bartolini, G., Prevost, G., Messeri, C., Carignani, G., Menini, UG., 1998, Olive germplasm. Cultivars and World-Wide collections. FAO, Rome, Italy. Belaj A., Satovic Z., Ismaeli H., Panajoti D., Rallo L. and Trujillo I. 2003a, RAPD genetic diversity of Albanian olive germplasm and its relationships with other Mediterranean countries. Euphytica 130: 387-395. Belaj A, Satovic Z, Cipriani G, Baldoni L, Testolin R, Rallo L, Trujillo I, 2003b, Comparative study of the discriminating capacity of RAPD, AFLP and SSR markers and of their effectiveness in establishing genetic relationships in olive, Theor Appl Genet 107:736-744. Botstein, D., White, R.L., Skolnick, M., Davis, R.W., 1980, Construction of a genetic map in man using restriction fragment length polymorphisms. Am J Hum Gen. 32:314–331. Cantini, C., Cimato, A., Sani, G., 1999, Morphological evaluation of olive germplasm present in Tuscany region. Euphytica, 109: 173-181. Carriero, F., Fontanazza, G., Cellini, F. and Giorio G., 2002, Identification of simple sequence repeats(SSRs) in olive (Olea europaea L.).Theor. Appl. Genet. 104: 301–307. Cordeiro, A.I.; Sanchez-Sevilla, J.F.; Alvarez-Tinaut, M.C. & Gomez-Jimenez, M.C., 2008, Genetic diversity assessment in Portugal accessions of Olea europaea by RAPD markers. Biologia Plantarum, 52, (4), 642-647. Doyle, J.J., Doyle, J.L., 1990, Isolation of plant DNA from fresh tissue. Focus, 12. Felsenstein J., 2007, PHYLIP (Phylogeny Inference Package) Version 3.67. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle. http://evolution.gs.washington.edu/phylip.html. Grati-Kamoun, N., Mahmoud, F., Rebai, A. and Gargouri, A., 2006, Genetic diversity of Tunisian olive tree (Olea europaea L.) cultivars assessed by AFLP markers. Genet. Resour. Crop Evol. 53: 265-275. Gülşen, O. ve Mutlu, N., 2005, Bitki biliminde kullanılan genetik markırlar ve kullanım alanları. Alatarım 4(2):27-37. La Mantia, M., Lain, O., Caruso, T., Testolin, R., 2005, SSR based DNA fingerprints reveal the genetic diversity of Sicilian olive (Olea europaea L.) germplasm. J. Hort. Sci. Biot. 80:628-632. Muzzalupo, I., Lombardo, N., Musacchio, A., Noce, M.E., Pellegrino, G., Perri, E. and Sajjad, A., 2006, DNA sequence analysis of microsatellite markers enhances their efficiency for germplasm management in an Italian olive collection, J Am Soc Hortic Sci 131(3):352-359. Muzzalupo, I., Stefanizzi, F., Perri, E., 2009, Evaluation of olives cultivated in southern Italy by simple sequence repeat markers, HortScience 44(3):582-588. Nei, M., 1978, Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics, 89, 583–590. Öztürk, F., 2006, Türkiye’de ve Dünya’da zeytincilik sektörünün genel görünümü. TAYEK, Ege tarımsal araştırma enstitüsü müdürlüğü, yayın no: 125, 45-62. Rallo, L., Barranco, D. and Escobar, F., 1997, El cultivo del olivo. Ediciones mundi prensa. pp. 701. Rekik, I., Salimonti, A., Grati-Kamoun, N., Muzzalupo, I., Perri, E., Rebai, A., 2008, Characterisation and identification of Tunisian olive tree varieties by microsatellite markers. HortScience 43:1371-1376. Remesal-Rodríguez, J., 1996, Economía oleícola: En la antigüedad, p. 47–58. In: consejo oleicola internacional (Ed.), ‘Enciclopedia mundial del olivo, plaza & Janes Editores S.A., Barcelona, Spain. SAS, 1995, SAS/STAT user’s guide. Version 6.12. SAS Institute, Cary, North Carolina. Sefc KM, Lopes MS, Mendonca D, Dos Santos MR., 2000, Identification of microsatellite loci in olive (Olea europaea) and their characaterization in Italian and Iberian olive trees. Mol. Ecol. 9: 1171-1173. Shahriari, M., Omrani, A., Falahati-Anbaran, A., Ghareyazei, B. & Nankali, A., 2008, Identification of Iranian olive cultivars by using RAPD and microsatellite markers. Acta Hort. 791:109-115. Vergarı G., Patumı M., Fontanazza G., 1996, Use of RAPDs markers in the characterisation of olive germplasm. Olivae, 60: 19-22. Yıldırım, A., Kandemir, N., 2001, Bitki biyoteknolojisi-II genetik mühendisliği ve uygulamaları, genetik markörler ve analiz metodları. Selçuk Üniversitesi Basımevi s:334- 363. Zamora, R., Alaiz, M., Hidalgo, F. J., 2001, Influence of cultivar and fruit ripening on olive (Olea europaea) fruit protein content, composition, and antioxidant activity. J. Agric. Food Chem, 49 (9), 4267-4270. Zohary, D., Spiegel-Roy, P., 1975, Beginning of fruit growing in the old world. Science, 187: 319-327. Zohary, D., Hopf, M., 1993, Domestication of plants in the old world. Oxford clarendon press, 137-143.
Details
| Subjects | Agricultural Engineering |
|---|---|
| Journal Section | Article |
| Authors | |
| Publication Date | June 30, 2017 |
| Published in Issue | Year 2017 Volume: 7 Issue: 1 |