Presence of ‘Candidatus Phytoplasma trifolii’ in yellowing and bushy appearance-associated pepper plants from Iğdır Province of Turkey

Abdullah Güller; Mustafa Usta; Gülüstan Korkmaz; Serap Demirel; Zeynelabidin Kurt

doi:10.20289/zfdergi.1081067

EN TR

Presence of ‘Candidatus Phytoplasma trifolii’ in yellowing and bushy appearance-associated pepper plants from Iğdır Province of Turkey

Abstract

Objective: The objective of this study was to investigate the group/subgroup of phytoplasma agent in peppers showing phytoplasma symptoms. Material and Methods: In this study, plants collected from Iğdır province in 2020 were analyzed using direct and nested PCR tests, and BLASTn, iphyClassifier, Mega 7, and pDRAW32 programs were used. Results: In the tests performed, approximately 1.2 kb of DNA fragments specific to phytoplasma were obtained. The 16S rRNA nucleotide sequence (1254 bp in length) (OM663745) revealed that it was showed more than 99.44% nucleotide similarity to other ‘Ca. P. trifolii’ members. The tentative RFLP and phylogenetic analyzes performed proved the ‘Ca. P. trifolii’ the infection from the Clover proliferation group (16SrVI) group and subgroup A in symptomatic pepper plants. Conclusion: The presence of ‘Ca. P. trifolii’ in naturally infected peppers in Iğdır province of Turkey was detected using PCR-RFLP and cladistic analysis.

Keywords

Clover proliferation group (16SrVI), Nested PCR, Capsicum annuum, RFLP

Türkiye’nin Iğdır İli’nde sararma ve çalımsı görünüm gösteren biber bitkilerinde ‘Candidatus phytoplasma trifolii’nin varlığı

Abstract

Amaç: Bu çalışmanın amacı fitoplazma belirtisi gösteren biberlerde fitoplazma etmeninin grup/alt grubunu araştırmaktır. Materyal ve Yöntem: Bu çalışmada, 2020 yılında Iğdır ilinden toplanan bitkiler direct ve nested PCR testleri kullanılarak analiz edilmiş ve BLASTn, iphyClassifier, Mega 7 ve pDRAW32 programları kullanılmıştır. Araştırma Bulguları: Testlerde, fitoplazmaya spesifik yaklaşık 1.2 kb DNA fragmentleri elde edilmiştir. Belirlenen 16S rRNA nükleotit dizisi (1254 bp) (OM663745) %99.44'ten fazla diğer ‘Ca. P. trifolii’ üyelerine nükleotit benzerliği göstermiştir. Gerçekleştirilen Sanal RFLP ve filogenetik analizler simptom gösteren biber bitkilerinde Clover proliferation group (16SrVI) grubu ve A alt grubundaki ‘Ca. P. trifolii’ etmeninin infeksiyonunu kanıtlamıştır. Sonuç: Türkiye’nin Iğdır ilinde doğal olarak enfekte olmuş biberlerde ‘Ca. P. trifolii’ varlığı PCR-RFLP ve kladistik analiz kullanılarak tespit edilmiştir.

Keywords

: Clover proliferation group (16SrVI), Nested PCR, Capsicum annuum, RFLP

Supporting Institution

Bulunmamaktadır

Thanks

Dergi Editörü ve sekretaryasına teşekkür ederim. Çalışmada emeği geçen tüm arkadaşlarıma teşekkür ederim.

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Details

Primary Language

English

Subjects

Agricultural, Veterinary and Food Sciences

Journal Section

Research Article

Authors

Abdullah Güller ^*
0000-0003-3887-4208
Türkiye

Mustafa Usta
0000-0002-3940-2774
Türkiye

Gülüstan Korkmaz
0000-0002-9032-5823
Türkiye

Serap Demirel
0000-0002-1877-0797
Türkiye

Zeynelabidin Kurt
0000-0003-1164-9326
Türkiye

Publication Date

September 30, 2022

Submission Date

March 1, 2022

Acceptance Date

May 17, 2022

Published in Issue

Year 2022 Volume: 59 Number: 3

DOI

https://doi.org/10.20289/zfdergi.1081067

IZ

https://izlik.org/JA89NR48EY

APA

Güller, A., Usta, M., Korkmaz, G., Demirel, S., & Kurt, Z. (2022). Presence of ‘Candidatus Phytoplasma trifolii’ in yellowing and bushy appearance-associated pepper plants from Iğdır Province of Turkey. Journal of Agriculture Faculty of Ege University, 59(3), 429-437. https://doi.org/10.20289/zfdergi.1081067

AMA

1.Güller A, Usta M, Korkmaz G, Demirel S, Kurt Z. Presence of ‘Candidatus Phytoplasma trifolii’ in yellowing and bushy appearance-associated pepper plants from Iğdır Province of Turkey. Journal of Agriculture Faculty of Ege University. 2022;59(3):429-437. doi:10.20289/zfdergi.1081067

Chicago

Güller, Abdullah, Mustafa Usta, Gülüstan Korkmaz, Serap Demirel, and Zeynelabidin Kurt. 2022. “Presence of ‘Candidatus Phytoplasma Trifolii’ in Yellowing and Bushy Appearance-Associated Pepper Plants from Iğdır Province of Turkey”. Journal of Agriculture Faculty of Ege University 59 (3): 429-37. https://doi.org/10.20289/zfdergi.1081067.

EndNote

Güller A, Usta M, Korkmaz G, Demirel S, Kurt Z (September 1, 2022) Presence of ‘Candidatus Phytoplasma trifolii’ in yellowing and bushy appearance-associated pepper plants from Iğdır Province of Turkey. Journal of Agriculture Faculty of Ege University 59 3 429–437.

IEEE

[1]A. Güller, M. Usta, G. Korkmaz, S. Demirel, and Z. Kurt, “Presence of ‘Candidatus Phytoplasma trifolii’ in yellowing and bushy appearance-associated pepper plants from Iğdır Province of Turkey”, Journal of Agriculture Faculty of Ege University, vol. 59, no. 3, pp. 429–437, Sept. 2022, doi: 10.20289/zfdergi.1081067.

ISNAD

Güller, Abdullah - Usta, Mustafa - Korkmaz, Gülüstan - Demirel, Serap - Kurt, Zeynelabidin. “Presence of ‘Candidatus Phytoplasma Trifolii’ in Yellowing and Bushy Appearance-Associated Pepper Plants from Iğdır Province of Turkey”. Journal of Agriculture Faculty of Ege University 59/3 (September 1, 2022): 429-437. https://doi.org/10.20289/zfdergi.1081067.

JAMA

1.Güller A, Usta M, Korkmaz G, Demirel S, Kurt Z. Presence of ‘Candidatus Phytoplasma trifolii’ in yellowing and bushy appearance-associated pepper plants from Iğdır Province of Turkey. Journal of Agriculture Faculty of Ege University. 2022;59:429–437.

MLA

Güller, Abdullah, et al. “Presence of ‘Candidatus Phytoplasma Trifolii’ in Yellowing and Bushy Appearance-Associated Pepper Plants from Iğdır Province of Turkey”. Journal of Agriculture Faculty of Ege University, vol. 59, no. 3, Sept. 2022, pp. 429-37, doi:10.20289/zfdergi.1081067.

Vancouver

1.Abdullah Güller, Mustafa Usta, Gülüstan Korkmaz, Serap Demirel, Zeynelabidin Kurt. Presence of ‘Candidatus Phytoplasma trifolii’ in yellowing and bushy appearance-associated pepper plants from Iğdır Province of Turkey. Journal of Agriculture Faculty of Ege University. 2022 Sep. 1;59(3):429-37. doi:10.20289/zfdergi.1081067

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https://doi.org/10.1111/epp.70009