Molecular characterization of tomato spotted wilt virus (TSWV) and cucumber mosaic virus (CMV) affecting tomato and pepper crops in Izmir Province

Nihan Güneş; İsmail Can Paylan; Mustafa Gümüş

doi:10.20289/zfdergi.1197925

EN TR

Molecular characterization of tomato spotted wilt virus (TSWV) and cucumber mosaic virus (CMV) affecting tomato and pepper crops in Izmir Province

Abstract

Objective: The objective of this study was to investigate tomato spotted wilt virus (TSWV) and cucumber mosaic virus (CMV) infections in tomato and pepper plants showing virus-induced symptoms in vegetable growing districts of İzmir, Turkey. Material and Methods: Surveys were carried out in tomato and pepper plantations in 2019 and 2021, and the incidences of these viruses in the collected leaf samples were determined by RT-PCR. Nucleotide identities and phylogenetic relationships of the TSWV and CMV isolates with other isolates retrieved from the GenBank database were determined. Results: The results of this study showed that tomato plants were infected at the same rate (21.50%) with TSWV and CMV. Out of the tested pepper samples, 64.15% were infected with TSWV and 25.47% with CMV. The results showed that, the identity rate of nucleoprotein region of TSWV isolates from tomato was 99-96% at nucleotide level while the isolates from pepper showed 100-95% identity. On the other hand, the capsid protein gene region of the tomato isolate of CMV had nucleotide identity rate of 98-95% with other isolates in GenBank, while that of its pepper isolates had 100-98% identity. Also, CMV isolates of this study showed close phylogenetic relationship with the CMV isolates of subgroup IB. Conclusion: This study revealed the prevalence of TSWV and CMV in symptomatic tomato and pepper samples in İzmir province and some molecular properties of them.

Keywords

Pepper , solanaceous crops , tomato , virus , RT-PCR

İzmir ilinde domates ve biber bitkilerini etkileyen tomato spotted wilt virus (TSWV) ve cucumber mosaic virus (CMV) etmenlerinin moleküler karakterizasyonu

Öz

Amaç: Bu çalışmanın amacı, İzmir'in sebze yetiştirilen önemli ilçelerinde virüs kaynaklı semptomlar gösteren domates ve biber bitkilerinde tomato spotted wilt virus (TSWV) ve cucumber mosaic virus (CMV) enfeksiyonlarının araştırılmasıdır. Materyal ve Yöntem: Domates ve biber üretim alanlarında 2019 ve 2021 yıllarında sürveyler yapılmış ve toplanan yaprak örneklerinde bu virüslerin enfeksiyon oranları RT-PCR ile belirlenmiştir. TSWV ve CMV izolatlarının GenBank veri tabanından elde edilen diğer izolatlarla benzerlik oranları ve filogenetik ilişkileri belirlenmiştir. Araştırma Bulguları: Çalışma sonucunda domates bitkilerinin aynı oranda (%21,50) TSWV ve CMV ile enfekteli olduğu belirlenmiştir. Test edilen biber örneklerinin %64,15'inin TSWV ve %25,47'sinin CMV ile enfekteli olduğu tespit edilmiştir. Sonuçlar, TSWV domates izolatlarının nükleokapsid protein gen bölgesinin nükleotid benzerlik oranının %99-96 olduğunu, TSWV biber izolatının ise %100-95 benzerlik gösterdiğini ortaya koymuştur. CMV-domates izolatının kapsid protein gen bölgesi GenBank'taki diğer izolatlarla %98-95 arasında, CMV-biber izolatınınki ise %100-98 arasında nükleotid benzerliğine sahip olmuştur. Ayrıca, CMV izolatları, alt grup IB CMV izolatları ile yakın filogenetik ilişki göstermiştir. Sonuç: Bu çalışma, İzmir ilinde simptomlu domates ve biber örneklerinde TSWV ve CMV'nin bulaşıklık oranını ve moleküler özelliklerini ortaya koymuştur.

Anahtar Kelimeler

Biber , RT-PCR , patlıcangil , domates , virüs

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Details

Primary Language

English

Subjects

Agricultural, Veterinary and Food Sciences

Journal Section

Research Article

Authors

Nihan Güneş ^*
0000-0002-6608-4871
Türkiye

İsmail Can Paylan
0000-0002-4815-5859
Türkiye

Mustafa Gümüş
0000-0002-1603-8666
Türkiye

Publication Date

April 1, 2023

Submission Date

November 1, 2022

Acceptance Date

February 17, 2023

Published in Issue

Year 2023 Volume: 60 Number: 1

DOI

https://doi.org/10.20289/zfdergi.1197925

IZ

https://izlik.org/JA92DU88FC

APA

Güneş, N., Paylan, İ. C., & Gümüş, M. (2023). Molecular characterization of tomato spotted wilt virus (TSWV) and cucumber mosaic virus (CMV) affecting tomato and pepper crops in Izmir Province. Journal of Agriculture Faculty of Ege University, 60(1), 67-79. https://doi.org/10.20289/zfdergi.1197925

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https://doi.org/10.1071/FP25105