In vitro and in vivo characterization of charcoal rot disease (Macrophomina phaseolina) in sesame
Abstract
Objective: This study aimed to characterize charcoal rot pathogen (Macrophomina phaseolina) of sesame in vitro and in vivo.
Material and Methods: In 2017, two isolates of M. phaseolina were isolated from symptomatic sesame plants in Aksu district of the Western Mediterranean region of Türkiye. Pathogenicity tests were conducted using cv. Muganlı-57 in a greenhouse. Micromorphological characteristics (colony growth, colony color, mycelium, and microsclerotium) and chlorate sensitivity of the isolates were determined in vitro. Effects of temperature on colony growth and symptoms of charcoal rot in the field were also examined in detail.
Results: Significant (P<0.01) differences were detected in microsclerotium length, microsclerotium width, and colony growths of the isolates on minimal medium. Both isolates were characterized as chlorate-sensitive. On minimal medium, Aksus1 isolate displayed a feathery growth pattern, while Aksus2 isolate showed a restricted growth pattern. Temperature significantly (P˂0.01) affected colony growth. The highest colony growths were detected at 35°C, while the lowest ones were found at 40°C.
Conclusion: The results may provide new insights into the epidemiology of the charcoal rot disease. To the best of our knowledge, this is the first detailed characterization of microscopic traits and charcoal rot symptoms on sesame in the field in the Western Mediterranean region of Türkiye.
Keywords
Field symptoms, fungal disease, fungal traits, Sesamum indicum
References
- Abd, S.W. & H.H. AL-Juboory, 2020. Evaluation of the activity of Bacillus subtilis and actinomyces sp. against Macrophomina phaseolina (TASSI) GOID. causative agent of charcoal rot on sesame. Plant Archives, 20 (1): 2343-2348.
- Ahmed, H.A.M., A.A. Abdel-Razik, M.H.A. Hassan & S.A. Khaled, 2010. Management of charcoal rot of sesame by seed soaking in medicinal plant extracts and hot water. The Plant Pathology Journal, 26 (4): 372-379.
- Ahmed, N.R. & M.M.A. Ibrahim, 2020. Influence of intercropping legume fodder crop guar with sesame for reducing charcoal-rot and wilt disease complex concerning to its yield and yield components. Annals of Agricultural Science, Moshtohor, 58 (3): 541-552.
- Akhtar, K.P., G. Sarwar & H.M.I. Arshad, 2011. Temperature response, pathogenicity, seed infection and mutant evaluation against Macrophomina phaseolina causing charcoal rot disease of sesame. Archives of Phytopathology and Plant Protection, 44 (4): 320-330.
- Amen, S.G., A.A. El-Sharawy, T.H.A. Hassan & M.Y. Abdalla, 2020. Use of biofumigation for controlling sesame root rot in North Sinai. Asian Journal of Plant Pathology, 14 (1): 21-26.
- Anonymous, 2023a. Meteoroloji Genel Müdürlüğü. Resmi İstatistikler. (Web page: https://www.mgm.gov.tr/veridegerlendirme/il-ve-ilceler-istatistik.aspx?m= ) (Date accessed: November 2023).
- Anonymous, 2023b. T.C. Çevre, Şehircilik ve İklim Değişikliği Bakanlığı. (Web page: https://www.mgm.gov.tr/veridegerlendirme/il-ve-ilceler-istatistik.aspx?m=ANTALYA ) (Date accessed: November 2023).
- Balabaskar, P., P. Renganathan & K. Sanjeevkumar, 2015. Occurrence of sesame root rot disease in Cuddalore district of Tamil Nadu and analysis of the variability in cultural characteristics and pathogenicity among isolates of Macrophomina phaseolina (Tassi) Goid. European Journal of Biotechnology and Bioscience, 3 (8): 55-59.
- Bashir, M.R., A. Mahmood, M. Sajid, M.A. Zeshan, M. Mohsan, Q.A.T. Khan & F.A. Tahir, 2017. Exploitation of new chemistry fungicides against charcoal rot of sesame caused by Macrophomina phaseolina in Pakistan. Pakistan Journal of Phytopathology, 29 (2): 257-263.
- Bedawy, I. & M. Moharm, 2019. Reaction and performance of some sesame genotypes for resistance to Macrophomina phaseolina, the incitant of charcoal rot disease. Alexandria Science Exchange Journal, 40 (1): 12-18.