Propagation of GF 677 (P. amygdalus x P. persica) Clone Rootstock in Tissue Culture by Shoot-tip Techique
Abstract
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Modified Murashige and Skoog (MS) nutrient medium was used to propagate the GF 677 clone rootstock by shoot tip technique. Survival ratio of shoot tips was differed between 38% and 60% and increased by BAP concentration. The best multiplication was obtained on a MS medium supplemented with 2.0 mg/l BAP, 0.1 mg/l NAA and 0.1 mg/l GA3. In general multiplication ration was increased when GA3 added to medium. Rooting of in vitro plantlets was better with NAA than IBA while there was no significant difference between auxins. The highest rooting was obtained on half or full strength MS medium containing 1.0 and 1.5 mg/l NAA or IBA. Half or full strength MS medium were showed similar affect on rooting.
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References
- Aghaye, M.N.R. and A. Yadollahi, 2012. Micropropagation of GF 677 rootstock. Journal of Agricultural Science, 4 (5): 131-138.
- Ahmad, T., H.U. Rahman, C.H. Ahmad and M.H. Laghari, 2003. Effect of culture media and growth regulators on micropropagation of peach rootstock GF 677. Pakistan Journal of Botany, 35(3): 331–338.
- Al-Tamimi M.O. and M.M. QrunBeh, 1996. Propagation of GF677 peach rootstock by stem cuttings. HortScience, 31(4).
- Anonymous, 2017. Rahan Meristem (1998) Ltd, Plant Propagation & Biotechnology. http://www.rahan.co.il/?page_id=146&lang= en. Erişim: Şubat, 2017.
- Antonopoulou, C., K. Dimassi, I. Therios, C. Chatzıssavidis and V. Tsirakoglou, 2005. Inhibitory effects of riboflavin (vitamin B2) on the ın vitro rooting and nutrient concentration of explants of peach rootstock GF 677 (P. amygdalus x P. persica). Biologia Plantarum, 48(4): 549-553.
- Arıcı, Ş.E. 2008. Bazı sert çekirdekli meyve anaçlarının doku kültürü ile çoğaltılması. Süleyman Demirel Üniversitesi Ziraat Fakültesi Dergisi, 3 (1): 19-23.
- Dakah, A., W. Mohsen and S. Zaid, 2014. Effect of nutrient media on in vitro micropropagation of GF-677 rootstock. Jordan Journal of Agricultural Sciences. 10 (3): 590-600.
- Dimassi Theriou, K., 1995. In vitro rooting of rootstock GF-677 (P. amygdalus x P. persica) as influenced by mineral concentration of the nutrient medium and type of culture-tube sealing material. Journal of Horticultural Science, 70 (1): 105-108.
- Dobránszki, J. and J.A. Teixeira da Silva, 2010. Micropropagation of apple – A review. Biotechnology Advances, 28, 462-488.
- Malavasi, F.F.F. and R. Ranieri, 1987. Preliminary investigation on in vivo rooting of microcuttings of GF-677 peach rootstock. Acta Horticulturae 212: :281-288.