Determination of Lentil (Lens culinaris M.) DEHYDRATION RESPONSIVE ELEMENT-BINDING2A (DREB2A) Gene Expression under Drought Stress Conditions

Year 2019, , 181 - 185, 27.06.2019

Melike Bakır

https://doi.org/10.20289/zfdergi.448332

Cited By: 1

Abstract

Objective: Dehydration responsive element binding (DREB) proteins are important transcription factors in plant stress response and signal transduction. In this study, partial cDNA of lentil (Lens culinaris M.) DREB2A, namely LcDREB2A, which belongs to the DREB gene family was isolated and identified its relationship with drought stress.

Material and Methods: Two-week-old plants were subjected to drought stress through irrigations for 6, 13 and 20 days to elucidate the relationship between the expression profile of LcDREB2A gene and drought stress. Changes of gene expression in lentil roots and leaves were assayed by quantitative real time PCR.

Results: The expression of LcDREB2A in both Fırat87 and Özbek lentil leaves increased with decreasing water contents and reached to a maximum on the 20th day of dehydration. On the other hand, in Özbek roots, the highest level of expression was observed on the 6th day of dehydration. The levels decreased on 13th day and increased on 20th day. Level of expression in Fırat87 roots decreased with increasing water contents and the greatest level of expression was observed on the 20th day of dehydration.

Conclusion: Observed tissue-specific expression profile of LcDREB2A suggested a complex regulation and indicated the role of this transcription factor in lentil drought response.

Keywords

Lentil, DREB2A, drought, gene expression, real-time quantitative PCR

Mercimek (Lens culinaris M.) DEHYDRATION RESPONSIVE ELEMENTBINDING2A (DREB2A) Geninin Kuraklık Stresi Koşullarındaki İfadesinin Belirlenmesi

Abstract

Amaç: Dehydration responsive element binding (DREB) proteinleri, bitkilerde strese yanıt ve sinyal iletiminde önemli rol oynarlar. Bu çalışmada, DREB gen ailesinin üyesi olan ve LcDREB2A olarak isimlendirilen mercimek (Lens culinaris M.) DREB2A geninin kısmi cDNA’sı izole edilmiş ve kuraklık stresi ile ilişkisi belirlenmiştir.

Materyal ve Metot: Kuraklık stresi ve LcDREB2A gen ifadesi arasındaki ilişkiyi anlamak için, 2 hafta süre ile yetiştirilen mercimek fidelerine 6, 13 ve 20 gün süre ile sulamama şeklinde kuraklık stresi uygulanmıştır. Kök ve yapraklarda meydana gelen gen ifadesi değişimleri eş zamanlı kantitatif PCR (Real-time qPCR) ile belirlenmiştir.

Bulgular: LcDREB2A gen ifadesi, Fırat87 ve Özbek çeşidinin yapraklarında artan kuraklık stresi ile artmış ve kuraklığın 20. gününde en yüksek seviyeye ulaşmıştır. Diğer taraftan Özbek çeşidinin köklerinde gen ifadesi seviyesi 6. günde en yüksek seviyeye ulaşırken 13. günde azalmış ve 20. günde tekrar artış göstermiştir. Fırat87 çeşidinde ise, artan kuraklık stresi ile birlikte düzenli bir artış görülmüş ve 20. günde en yüksek seviyeye ulaşmıştır.

Sonuç: LcDREB2A geninde gözlenen doku spesifik gen ifadesi profili, bu transkripsiyon faktörünün mercimek bitkisinde kuraklık stresindeki rolünü ve kompleks regülasyonunu göstermektedir.

Keywords

Mercimek, DREB2A, kuraklık, gen ifadesi, eş zamanlı kantitatif PCR

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