Effects of Nitrogen Fixing and Phosphate Solubilizing Bacteria on Growth and Bulbs Production of Tulip Cultivars

Year 2019, , 241 - 248, 27.06.2019

Fazilet Parlakova Karagöz , Atilla Dursun

https://doi.org/10.20289/zfdergi.477345

Cited By: 5

Abstract

Objective: This study was under taken to determine the effects of nitrogen fixing and phosphate solubilizing bacteria on plant development, number of bulb and quality of bulb of some tulipcultivars.

Materials and Methods: The research was conducted in open field conditions, in 2013. Pink Impression, Blue Aimable and Golden Parade varieties of Tulipa gesneriana L. were used as plant materials. Tulip bulbs were inoculated by four different bacterial formulations comprising Pantoea agglomerans RK-79, Pantoea agglomerans RK-92, Bacillus megaterium TV-91C, Bacillus subtilis TV-17C, Bacillus megaterium TV-3D, Paenibacillus polymyxa TV-12E, Bacillus megaterium TV-6D, Pseudomonas putida TV-42A bacteria strains.

Results: According to research results, significant results were determined based on among the applications and varieties. According to applications, average maximum number mother bulbs was obtained from cultivar of Golden Parade while average maximum number bulblet was observed in Blue Aimable cultivar. The highest average maximum bulbet was obtained from formulation C application in Blue Aimable cultivar.

Conclusion: As a result of the study, it has been concluded that the bacterial formulation applications especially formulation C (RK-79 + RK-92 + TV-3D + TV-12E) application increased inthe number of bulbs and quality of the cultivars.

Keywords

Bulblet, PGPR, Rhizobacteria formulations, Tulipa gesneriana L.

Azot Fikseri ve Fosfat Çözücü Bakterilerin Lale Çeşitlerinin Gelişimi ve Soğan Üretimine Etkisi

Abstract

Amaç: Bu çalışma azot fikseri ve fosfat çözücü bakteri formülasyonlarının bazı lale çeşitlerinde bitkisel özellikler, soğan sayısı ve soğan kalitesi üzerine etkilerini araştırmak amacıyla yapılmıştır.

Materyal ve Yöntem: Çalışma, 2013 yılında tarla koşullarında yürütülmüştür. Araştırmada bitkisel materyal olarak, Tulipa gesneriana L. türüne ait Pink Impression, Blue Aimable ve Golden Parade üç farklı lale çeşidi kullanılmıştır. Lale soğanları, Pantoea agglomerans RK-79, Pantoea agglomerans RK-92, Bacillus megaterium TV-91C, Bacillus subtilis TV-17C, Bacillus megaterium TV-3D, Paenibacillus polymyxa TV-12E, Bacillus megaterium TV-6D, Pseudomonas putida TV-42A bakteri ırklarını içeren

dört farklı bakteriyel formülasyon ile aşılanmıştır.

Bulgular: Araştırma bulgularına göre, uygulamalar ve çeşitler arasında önemli sonuçlar elde edilmiştir. Belirlenen ortalama en fazla ana soğan sayısı Golden Parade çeşidinde elde edilirken ortalama en fazla yavru soğan sayısı ise Blue Aimable çeşidinde elde edilmiştir. Ortalama en fazla yavru soğan sayısı Blue Aimable çeşidinde ve formülasyon C uygulamasında elde edilmiştir.

Sonuç: Çalışma sonucunda bakteri formülasyon uygulamalarıyla, özellikle formülasyon C (RK-79 + RK-92 + TV-3D + TV-12E) uygulamasının soğan sayısı ve kalitesinin arttırılabileceği sonucuna varılmıştır.

Keywords

PGPR, Tulipa gesneriana L., Rizobakteri formülasyonu, Yavru soğan

References

• Aksoy, U. And Altındişli, A. 1998. Ekolojik (organik, biyolojik) Tarım (Ecological (Organic, Biological) Agriculture). Publications of Ecological Agriculture Organization (ETO), Bornova-İzmir, 125.
• Atay, S. 1996. Soğanlı Bitkiler (Onion Plants). Guide to the Promotion and Production of Species Exported from Turkey. Natural Life Protection Association, ISBN 975-96081-1-1-1, 84s, Istanbul.
• Başkent, A. 2008. The Effects of Different Aggregates on the Formation and Characteristics of Tulip Bulb at the Ring Culture. [Master Thesis], Ankara University Graduate School of Natural and Applied Sciences Department of Horticulture in Ankara: 1-53.
• Bhattacharyya, P.N. and Jha, D.K. 2012. Plant Growth-Promoting Rhizobacteria (PGPR): Emergence in Agriculture. World J. Microbiol. Biotechnol. 28(4):1327-1350. https://doi.org/10.1007/s11274-011-0979-9
• Chandler, D., Davidson, G., Grant, W.P., Greaves, J. and Tatchell, G.M. 2008. Microbial Biopesticides for Integrated Crop Management: An Assessment of Environmental and Regulatory Sustainability. Trends Food Sci. Tech. 19:275–283. https://doi.org/10.1016/j.tifs.2007.12.009
• Dole, J.M., Wilkins, H.F. 1999. Floriculture Principles and Species. H. ISBN: 0-13-374703-4, Prentice Hall Inc., Printed USA.
• Eid, A.R., Awad, M.N. and Hamouda, H.A. 2009. Evaluate Effectiveness of Bio and Mineral Fertilization on the Growth Parameters and Marketable Cut Flowers of Matthiola incana L. Am-Eur,. J. Agr. Environ. Sci. 5(4):509–518.
• Erman, M., Kotan, R., Çakmakçı, R., Çığ, F., Karagöz, F. and Sezen, M. 2010. Effects of Nitrogen Fixation and Phosphate Solubilizing Bacteria Isolated from the Van Lake Basin on Growth and Yield Characteristics of Wheat and Sugar Beet. Turkey IV. Organic Agriculture Symposium, June 28 - July 1, 2010. Erzurum. 325-329.
• Flores-Félix, J.D., Menéndez, E., Rivera, L.P., Marcos‐García, M., Martínez‐Hidalgo, P., Mateos, P.F., Martinez-Molina, E., Velazquez, M.D.L.E., Garcia-Fraile, P. and Rivas, R. 2013. Use of Rhizobium leguminosarum as a Potential Biofertilizer for Lactuca sativa and Daucus carota Crops. J. Plant Nutr. Soil. Sci. 176(6):876–882. https://doi.org/10.1002/jpln.201300116
• García-Fraile, P., Carro, L., Robledo, M., Ramírez-Bahena, M.H., Flores-Félix, J.D., Fernández, M.T., Mateos, P.F., Rivas, R., Igual, J.M., Martinez-Molina, E., Peix, Á. and Velazquez, E. 2012.) Rhizobium Promotes Non-Legumes Growth and Quality in Several Production Steps: Towards a Biofertilization of Edible Raw Vegetables Healthy for Humans. PLoS ONE 7(5):e38122.
• Gezgin, B. 2007. The Effects of Different Pot Size on the Formation and Characteristics of Tulip Bulb at the Ring Culture. [Master Thesis], Ankara University Graduate School of Natural and Applied Sciences Department of Horticulture in Ankara: 1-49.
• Göre, M.E. and Altin, N. 2006. Growth Promoting of Some Ornamental Plants by Root Treatment with Specific Fluorescent Pseudomonads. J. Biol. Sci. 6(3):610-615.
• Gunes, A., Karagoz, K., Turan, M., Kotan, R., Yildrim, E., Cakmakci, R. and Sahin, F. 2015. Fertilizer Efficiency of Some Plant Growth Promoting Rhizobacteria for Plant Growth. Res. J. Soil Biol. 7(2): 28-45. doi: 10.3923/rjsb.2015.28.45
• Jeon, J.S., Lee, S.S., Kim, H.Y., Ahn, T.S. and Song, H.G. 2003. Plant Growth Promotion in Soil by Some Inoculated Microorganisms. J. Microbiol. 41:271- 276.
• Karagöz, K. and Kotan, R. (2010). Bitki gelişimini teşvik eden bazı bakterilerin marulun gelişimi ve bakteriyel yaprak lekesi hastalığı üzerine etkileri. Türkiye Biyolojik Mücadele Dergisi, 1(2), 165-179.
• Karakurt, H., Kotan, R., Daddasoglu, F., Aslantas, R. and Sahin, F. 2011. Effects of Plant Growth Promoting Rhizobacteria (PGPR) on Fruit Set, Pomological and Chemical Characteristics, Color Values, and Vegetative Growth of Sour Cherry (Prunus cerasus cv. Kütahya). Turk. J. Biol., 35:283-291. doi:10.3906/biy-0908-35
• Kloepper, J.W. and Schroth, M.N. 1981. Relationship of in Vitro Antibiosis of Plant Growth Promoting Rhizobacteria to Plant Growth and the Displacement of Root Microflora. Phyto., 71:1020–1024.
• Kotan, R., Çakmakçı, R., Şahin, F., Karagöz, K., Dadaşoğlu, F. and Kantar, F. 2010. Biological Fighting Practices for the Control of Diseases and Harms by Using Bacterial Bioassays in Turkey. Turkey IV. Organic Agriculture Symposium, 28 June-1 July 2010, Erzurum. 726-738.
• Kotan, R., Kant, C., Karagöz, K., Dadaşoğlu, F., Çakmakçı, R., Fayetörbay, D., Şahin, F. and Çomaklı, B. 2009. The Influence of Some Bacterial Inoculations on the Growth and Chemical Composition of Alfalfa (Medicago sativa L.) under Controlled Conditions. 16th National Biotechnology Congress, 13-16 December 2009, Antalya. p.14.
• Kumar, R., Ahmed, N., Singh, D.B., Sharma, O.C., Lal, S. and Salmani, M.M. 2013. Enhancing Blooming Period and Propagation Coefficient of Tulip (Tulipa gesneriana L.) Using Growth Regulators. Afr. J Biotechnol., 12(2):168-174. http://dx.doi.org/10.5897/AJB12.2713
• Le Nard, M. and De Hertogh, A.A. 1993. Bulb Growth and Development and Flowering. The Physiology of Flower Bulbs. Elsevier, Amsterdam: 29-43.Minorsky, P.V. 2008. On the Inside. Plant Physiol 146: 323–324. www.plantphysiol.org/cgi/doi/ 10.1104/pp.104.900278
• Parlakova-Karagöz, F., Dursun, A., Kotan, R., Ekinci, M., Yıldırım, E. and Mohammadı, P. 2016. Assessment of the Effects of Some Bacterial Isolates and Hormones on Corm Formation and Some Plant Properties in Saffron (Crocus sativus L.). J. Agric. Sci. 22(4):500-511.
• Rees, A.A. 1971. Factors Affecting the Growth of Daughter Bulbs in the Tulip. Ann. Bot., 35:43-55.
• Sharma, S. and Kaur, M. 2010. Antimicrobial Activities of Rhizobacterial Strains of Pseudomonas and Bacillus Strains Isolated from Rhizosphere Soil of Carnation (Dianthus caryophyllus cv. Sunrise). Ind. J. Microbiol 50(2):229–232.
• Srivastava, R. and Govil, M. (2007). Influence of biofertilizers on growth and flowering in gladiolus cv. American beauty. Acta Hortic., 742:183–188.
• Ürgenç, S.İ. 1998). Tree and Ornamental Nursery Nursery and Cultivation Technique. Istanbul University Faculty of Forestry Publications, Faculty Publication No: 442, ISBN 975-404- 445-7, İstanbul.
• Van der Meulen-Muisers, J.J.M., Van Oeveren, J.C. and Van Tuyl, J.M. 1996. Breeding as a Tool for Improving Postharvest Quality Characters of Lily and Tulip Flowers. VII International Symposium on Flowerbulbs 430.
• Van Loon L.C. 2007. Plant Responses to Plant Growth-Promoting Rhizobacteria. Eur. J. Plant Pathol., 119(3):243–254. doi:10.1007/s10658007-9165-1
• Zulueta-Rodriguez, R., Cordoba-Matson, M.V., Hernandez-Montiel, L.G., Murillo-Amador, B., Rueda-Puente, E. and Lara, L. 2014. Effect of Pseudomonas putida on Growth and Anthocyanin Pigment in Two Poinsettia (Euphorbia pulcherrima) Cultivars. The Scientific World Journal Article ID 810192. http://dx.doi.org/10.1155/2014/810192