Reaction of Different Inoculation Densities of Meloidogyne incognita race 2 on Some Resistant Pepper lines

Year 2018, Volume: 55 Issue: 2, 161 - 170, 27.06.2018

Fatma Gül Göze Özdemir , Gülsüm Uysal

https://doi.org/10.20289/zfdergi.408828

Cited By: 2

Abstract

Reaction of
different inoculation densities of two Meloidogyne
incognita race2 isolates (G7 and K4) was
studied on the root-knot nematode resistant pepper lines Carolina Wonder, PM217,
CM334 and susceptible Yolo Wonder
under controlled conditions (25±2°C temperature, 60±5% humidity and 16:8
photoperiodizm). Experiments were conducted on a randomized block design with 5
replications. Egg masses were used inoculum source and when the plants reached
4 real leaf seedlings, 1-5-10-20 and 50 egg masses were inoculated into the 2
cm depth of plant root zone. Peppers were harvested 9 weeks after inoculation
and root gall ratio was indexed according to 0-5 scalae. Numbers of galls, egg
masses and eggs in a roots were counted under light microscopy and reproduction
fitness were evaluated. G7 and K4
isolates showed avirulent reaction in all inoculum densities in Carolina Wonder
and CM334 resistant pepper lines. In the PM217 resistant pepper line, Reproduction
fitness of G7 isolate was found 1.1 in the density of 50 egg masses inoculum
densities, and the durability of the pepper line is broken.10-20 and 50 egg
mases inoculum densities of K4 isolate showed virulent reaction in the PM217
resistant pepper line. Reproduction fitness in these inoculum densities were
1.4, 3.5 and 4.4 respectively. The
pathogenicity of both isolates in the Yolo Wonder pepper line was found to be
high.

Keywords

Meloidogyne incognita race2, pepper, resistance, virulence, inoculum density, pathogenicity

Meloidogyne incognita ırk 2'nin Farklı İnokulasyon Yoğunluklarının Bazı Dayanıklı Biber Hatlarında Reaksiyonu

Abstract

Çalışmada, Meloidogyne incognita ırk 2'nin iki izolatı (G7 ve K4) 'nın farklı inokulasyon
yoğunluklarının Kök-ur nematodlarına dayanıklı Carolina Wonder, PM 217,
CM334 biber hatları ile M. incognita
'ya duyarlı Yolo Wonder hattında reaksiyonu kontrollü koşullar altında (25±2°C sıcaklık, %
60±5 nem ve 16:8 fotoperiyot) araştırılmıştır. Denemeler, tesadüf blokları deneme desenine
göre 5 tekerrürlü olarak kurulmuştur. İnokulum kaynağı olarak yumurta paketi kullanılmış ve bitkiler 4 gerçek
yapraklı fide dönemlerine geldiklerinde bitki kök bölgesinde açılan 2 cm derinliğe 1-5-10-20 ve 50 yumurta paketi inokule edilmiştir.
Biber bitkileri inokulasyondan 9 hafta sonra sökülmüş ve köklerdeki gal oranı 0-5
skalasına göre indekslenmiştir. Köklerde gal sayısı, yumurta paketi ve yumurta
sayısı ışık mikroskobu altında sayılmış ve üreme gücü hesaplanmıştır. Carolina Wonder ve CM334  dayanıklı biber hatlarında G7 ve K4 izolatı
tüm inokulum yoğunluklarında avirülent reaksiyon göstermiştir. PM217 dayanıklı
biber hattında G7 izolatının 50 yumurta paketi inokulum yoğunluğunda üreme
oranı 1.1 olarak bulunmuş ve dayanıklı biber hattında dayanıklılığı kırdığı saptanmıştır.
K4 izolatı PM217 dayanıklı biber hattında 10-20 ve 50 yumurta paketi inokulum
yoğunluğunda virülent reaksiyon göstermiştir. Bu inokulum yoğunluklarında üreme
oranı sırasıyla 1.4,3.5 ve 4.4 olarak bulunmuştur. Yolo Wonder biber hattında
her iki izolatın patojenitesi yüksek bulunmuştur.

Keywords

Meloidogyne incognita ırk2, biber, dayanıklılık, virülenslik, inokulum yoğunluğu, patojenite

References

• Barker, K.R. and T.H.A. Olthof. 1976. Relationships between nematode population densities and crop responses. Annuel review Phytopathology, 14: 327-353.
• Bommalinga, S., B.M.R. Reddy, N.G. Ravichandra, D.M. Preethi, P.S. Prasad and A.C. Kiran Kumar. 2013. Bio-Management of Meloidogyne incognita on bell pepper (Capsicum annuum) using different bio-agents. Indian Journals Bioinfolet, 10: 55-58.
• Castagnone-Sereno, P., M. Bongiovanni, and A. Dalmasso. 1992. Differential expression of root-knot resistance genes in tomato and pepper: evidence with virulent and avirulent near-isogenic lineages. The Annals of Applied Biology, 120, 487–492.
• Castagnone-Sereno, P., E. Wajnberg, M. Bongiovanni, F. Leroy, and A. Dalmasso. 1994. Genetic variation in Meloidogyne incognita virulence against the tomato Mi resistance gene: evidence from isofemale line selection studies. Theoretical and Applied Genetics. 88, 749–753.
• Castagnone-Sereno, P., M. Bongiovanni, and C. Djian-Caporalino. 2001. New data on the specificity of the root-knot nematode resistance genes Me1 and Me3 in pepper. Plant Breeding, 120, 429-433.
• Chandra, P., R. Sao, S.K. Gautam and A.N. Poddar. 2010. Initial population density and its effect on the pathogenic potential and population growth of the root knot nematode Meloidogyne incognita in four species of cucurbits. Asian Journal of Plant Pathology, 4(1):1-15.
• Dalmasso, A., M.C. Cardin, E. Pochard and M.C. Daunay. 1985. Pouvair pathogene des nematods Meloidogyne et genetique de la resistance chez quelques solanacees maraicheres. Comptes Rendus des Seances de I’academie d’ Agriculture de France, 71: 771-779.
• Devran, Z. and M.A. Söğüt. 2009. Distribution and İdentification of Root-knot nematodes from Turkey. Journal of Nematology, 41(2): 128-133.
• Di Vito, M., N. Greco and A. Carella. 1985. Population densities of Meloidogyne incognita and yield of Capsicum annuum L. Journal of Nematology, 17:45-49.
• Di Vito, M., F. Saccardo and G. Zacheo. 1991. Response of lines of Capsicum spp. To Italian populations of four species of Meloidogyne. Nematologia Mediterranea, 19: 43-46. Di Vito, M., V. Cianciotta and G. Zacchea. 1992. Yield of susceptible and resistant pepper in microplots infested with Meloidogyne incognita. Nematropica, 22: 1-6.
• Di Vito, M., N. Vovlas and P. Castillo. 2004. Host parasite relationships of Meloidogyne incognita on spinach. Plant pathology, 53:508-514.
• Djian- Caporalino, C., L. Pijarowski, A. Januel, V. Lefebvre, A. Daubeze, A. Palloıx, A. Dalmasso and P. Abad. 1999. Spectrum of resistance to root knot nematodes and inheritance of heat-stable resistance in pepper (Capsicum annuum L.) . Theoretical and Applied Genetics, 99: 496-502.
• Djian-Caporalino, C., L. Pijarowski, A. Fazari, M. Samson, L. Gaveau, C. 0’Byrne, V. Lefebvre, C. Caranta, A. Palloıx, and P. Abad, 2001, High-resolution genetic mapping of the pepper (Capsicum annuum L.) resistance loci Me3 and Me4 conferring heat-stable resistance to root knot nematodes (Meloidogyne spp.). Theoretical and Applied Genetics, 103, 592-600.
• Djian-Caporalino, C., A. Fazari, M.J. Arguel, T. Vernie, C. Vande Casteele, I. Faure, G. Brunoud, L. Pijarowski, A. Palloix, V. Lefebvre, and P. Abad, 2007. Root-knot nematode (Meloidogyne spp.) Me resistance genes in pepper (Capsicum annuum L.) are clustered on the P9 chromosome. Theoretical and Applied Genetics, 114, 473-486.
• Djian-Caporalino, C., S. Molinari, A. Palloix, A. Ciancio, A. Fazari, N. Marteu, N. Ris and P. Castagnone-Sereno. 2011. The reproductive potential of the root knot nematode Meloidogyne incognita is affected by selection for virulence against major resistance genes from tomato and pepper. European Journal Plant Pathology, 131: 431-440.
• Djian-Caporalino, C., A. Palloix, A. Fazari, N. Marteu, A. Barbary, P. Abad, A.M. Sage-Palloix, T. Mateille, S. Risso, R. Lanza, C. Taussig, and P. Castagnone-Sereno. 2014. Pyramiding, alternating or mixing comperative performances of deployment strategies of nematode resistance genes to promote plant resistance efficiency and durability. Biomedcentral Plant Biology, 14-53.
• Fazari, A., A. Palloıx, L. Wang, M. Hua, A. Sage-Palloıx, B. Zhang and C. Djıan- Caporalino. 2012. The root-knot nematode resistance N- gene co-localizes in the Me- genes cluster on the pepper (Capsicum annuum L.) P9 chromosome. Plant Breeding, 131: 665-673.
• FAO, 2012. // http: www.faostat.fao.org. Erişim tarihi: Kasım, 2012.
• Fery, R.L., and P.D. Dukes. 1984. ‘Carolina Cream’ southernpea. HortScience, 19: 456-457.
• Fery, R. L., P.D. Dukes and J.A. Thies. 1998. ‘Carolina Wonder’ and ‘Charleston Belle’: Southern root-knot nematode resistant bell peppers. HortScience, 33: 900-902.
• Fourie, H., A.H. Mc Donald and D. De Waele. 2010. Relationships between initial population densities of Meloidogyne incognita race 2 and nematode population development in terms of variable soybean resistance. Journal of Nematology, 42(1):55–61.
• Gergon, E.B., S.A. Miller, J.M. Halbrend and R.G. Davide. 2002. Effect of rice root-knot nematode on growth and yield of Yellow Granex. Onion. Plant Disease, 86: 1339-1344.
• Giri Babu, P., R.V. Singh and A.D. Munshi. 2008. Pathogenicity of root-knot nematode (Meloidogyne incognita race 1) on Ridge gourd (Luffa acutangub) and sponge gourd (Luffa cylindrica). Indian Journal of Nematology, 38: 255-257.
• Göze, F.G., 2014. Nematoda Dayanıklı Bazı Biber Gen Kaynaklarında Kök-ur Nematodu (Meloidogyne spp.) Popülasyonlarının Reaksiyonlarının Belirlenmesi. S.D.Ü. Fen Bil. Ens. Yüksek Lisans Tezi, 112s, Isparta.
• Hare, W.W. 1956. Comparative resistance of seven pepper varieties to five root-knot nematodes. Phytopathology, 46:669-672.
• Hare, W.W. 1966. New pimiento is resistant to nematodes. Miss. Farm Res. 29(2): 1-8.
• Hartman, K.M. and J.N. Sasser. 1985. Identification of Meloidogyne species on the basis of differential host test and perineal pattern morphology. In: Barker, K.R., Carter, C.C., Sasser, J.N. (Eds.), An Advanced Treatise on Meloidogyne. Methodology, vol. II. North Carolina State University Graphics, North Carolina, pp. 69-77.
• Hendy, H., E. Porchard and A. Dalmasso. 1983. Identification de 2 nouvelle sources de resistance aux nematodes du genre Meloidogyne chezle pimenta Capsicum annuuum L., CR Seances Acad Agric. Fraudes, 69: 817-822.
• Hendy, H., A. Dalmasso, and M.C. Cardin. 1985. Differences in resistant Capsicum annuum attacked by different Meloidogyne species. Nematologica, 31: 72-78. Jones, F.G.W. 1956. Soil population studies using microplots. Nematologica, 1: 109-110.
• Kepenekçi, İ., E. Evlice, A. Aşkın, M. Özakman and B. Tunalı. 2009. Burdur, Isparta ve Eskişehir illerindeki örtüaltı sebze yetiştiriciliğinde sorun olan kök-ur nematodları (Meloidogyne spp.)’nın fungal ve bakteriyel patojenlerinin belirlenmesi üzerine araştırmalar. Bitki Koruma Bülteni, 49 (1): 21-30.
• Khan, A.A. and M.W. Khan. 1991. Suitabilty of some cultivars of pepper as host for Meloidogyne javanica and races of M. incognita. Nematology Medirerranean, 19: 51-53.
• Khan, T.A., M.S. Ashraf and S. Hasan. 2006. Pathogenicity and life cycle of Meloidogyne javanica on Balsam (Impatiens balsamina). Arch.Phytopathology Plant Protection, 39:45-48.
• Kokalis –Burelle, N., M.G. Bausher and E.N. Rosskopf. 2009. Greenhouse evaluation of Capsicum rootstocks for management of Meloidogyne incognita on grafted bell pepper. Nematrophica, 39: 121-132.
• Kumar, M. and K.N. Pathak. 2005. Influence of Meloidogyne incognita on germination, seedling emergence and plant growth of lettuce, Lactuva sativa Linn., Ann. Plant Protection Society, 13:224-229.
• Lefebvre, V., A. Palloix, and M. Rives, 1993. Nuclear RFLP between pepper cultivars ( Capsicum annuum L.). Euphytica, 71, 189-199.
• Lefebvre, V., B. Goffinet, J.C. Chauvet, B. Caromel, P. Signoret, R. Brand, and A. Palloix. 2001. Evaluation of genetic distances between pepper inbred lines for cultivar protection purposes; comparison of FLP, RAPD and phenotypic data. Theoretical and Applied Genetics, 102, 741-750.
• Lindsey, D.L. and M.S. Clayshulte. 1982. Influence of initial population densities of Meloidogyne incognita on three chile cultivars. Journal of Nematology, 14: 353-358.
• Mekete, T., W. Mandefro and N. Greco. 2003. Relationship between initial population densities of Meloidogyne javanica and damage to pepper and tomato in Ethiopia. Nematology Mediterranean, 31:169-171.
• Morgan, G. D., W.R. Stevenson, A. E. MacGuidwin, K.A. Kelling, L.K. Binning and J. Zhu. 2002. Plant pathogen population dynamics in potato fields. Journal of Nematology, 34(3):189–193.
• Parveen, K., A. Haseeb and P.K.I. Shukla. 2006. Pathogenic potential of Meloidogyne incognita on Mentha arvensis cv. Gomti. Indian Journal of Nematology, 36:177-180.
• Ogunfowora, A.O. 1977. Effect of different population levels of Meloidogyne incognita on the yield of Tomato (Lycopersicon esculentum) in the Southwestern. Nigera Plant Protection, 3:61-67.
• Oka, Y., R. Offenbach and S. Pivonia. 2004. Pepper rootstock graft compatibility and response to Meloidogyne javanica and M. incognita. Journal of Nematology, 36(2):137-141.
• Olthof, T.H.A. and J.W. Potter. 1973. The relation- ship between population densities of Pratylenchus penetrans and crop losses in summer-maturing vegetables in Ontario. Phytopathology, 63:577-582.
• Özarslandan, A., H. Pınar, A. Ata, and D. Keleş. 2015. Resistance of pepper lines against Meloidogyne incognita. Türkiye Entomoloji Dergisi, 39 (2): 209-215.
• Sasser, J.N. and C.C. Carter. 1985. Overview of the International Meloidogyne Project, 1974-1984. J.N., Sasser, C.C., Carter (eds.). An Advanced Treatise on Meloidogyne: Volume 1, Biology and Control. North Carolina State University Raphics,19-24.
• Sasser, J.N. and D.W. Freckman. 1987. A world prospective on nematology: the role of the society, pp. 7-14 in Vistas on Nematology edited by J.A. Veech and Dickson Society of Nematologists, Hyattsville, M.D.
• Seinhorst J.W. 1965. The relation between nematode densi- ties and damage to plants. Nematologica, 11: 137-154.
• Shafiee, M.F. and W.R. Jenkins. 1963. Host parasite relationships of Capsicum frutescens and Pratylenchus penetrans, Meloidogyne incognita acrita and M. hapla. Phytopathology, 53: 325-328.
• Sikora, R.A. J. Bridge, and J.L. Starr. 2005. Management practices: an overview of integrated nematode management technologies. In Luc M, Sikora, R.A, Bridge J.(eds) Plant parasitic nematodes in subtropical and tropical agriculture. CABI, 319-392.
• Sorribas, F. J., C. Ornat, S. Verdejo-Lucas, M. Galeano and J. Valero. 2005. Effectiveness and profitability of the Mi resistant tomatoes to control root-knot nematodes. European Journal of Plant Pathology, 111: 29–38.
• Söğüt, M.A. ve İ.H. Elekçioğlu. 2000. Akdeniz Bölgesi’nde sebze alanlarında bulunan Meloidogyne Goeldi, 1892 (Nemata: Heteroderidae) türlerinin ırklarının belirlenmesi. Türkiye Entomoloji Dergisi, 24 (1): 33-40.
• Söğüt, M.A. ve İ.H. Elekçioğlu. 2007. Methyl Bromide alternatives for controlling Meloidogyne incognita in pepper cultivars in the Eastern Mediterranean region of Turkey. Turkish Journal of Agriculture and Forestry, 32 (1): 31-40.
• Stirling, G.R. 1991. Biological control of plant-parasitic nematodes. Wallingford, UK, CAB International. 282.
• Swarup, G. and R.D. Sharma. 1965. Root- knot of vegetables. IV. Relation between pop- ulation density of Meloidogyne javanica and Meloidogyne incognita var. acrita, and root and shoot growth of tomato seedlings. Indian Journal of Experimental Biology, 3:197-198.
• Thomas, S.H. and M. Cardenas. 1985. Relationship between preseason numbers of Meloidogyne incognita and yield losses in Chili pepper cultivar. Phytopathology, 75:1304.
• Thies, J.A., J.D. Mueller and R.L. Fery. 1997. Effectiveness of resistance to southern root knot nematode in Carolina Cayenne pepper in greenhouse, microplot, and field tests. Journal of American Society of Horticultural Science, 122:200-204.
• Thies, J.A., J.D. Mueller and R.L. Fery. 1998. Use of a resistant pepper as a rotational crop to manage southern root-knot nematode. HortScience, 33: 716–718.
• Thies, J.A. and R.L. Fery. 2000. Characterization of resistance conferred by N gene to Meloidogyne arenaria races 1 and 2, M. hapla, and M. javanica. Journal of American Society of Horticultural Science, 125:71-75.
• Thies, J.A. and R.L. Fery. 2002a. Heat stability of resistance to southern root-knot nematode in bell pepper genotypes homozygous and heterozygous for the N gene. Journal of American Society of Horticultural Science, 127:371-375.
• Thies, J.A. and R.L. Fery. 2002b. Evaluation of core of the U.S. Capsicum Germplasm collection for reaction to the Northern root knot nematode. HortScience, 37(5): 805-810.
• Thies, J. A., 2011. Virulence of Meloidogyne incognita to expression of N gene in pepper. Journal of Nematology, 43 (2), 90-94.
• Vovlas, N., G. Lycorelli, N. Sasonelli, A. Trocoli, I.C. Papajova, J.E. Palomores-Rius and P. Castillo. 2008. Pathogenicity and host-parasite relationships of the root-knot nematode Meloidogyne incognita on Celery. Plant Pathology, 57:981-987.
• TÜİK, 2012. https://biruni.tuik.gov.tr/bitkiselapp/bitkisel.zul. Erişim tarihi: Kasım, 2012.
• Wallace, H.R. 1973. Nematode ecology and Plant Disease. Edward Arnold publishers Ltd., London, ISBN:0844802719, pp:228.
• Wang, L.H., X.H. Gu, M.Y. Hua, S.L. Mao, Z.H. Zhang, D.L. Peng, X.F. Yun, and B.X. Zhang, 2009. A SCAR marker linked to the N gene for resistance to root knot nematodes (Meloidogyne spp.) in pepper (Capsicum annuum L.). Scientia Horticulturae, 122, 318–322.
• Williamson, V.M and P.A. Roberts. 2009. Mechanisms and genetics of resistance. In Perry RN, Moens M. Starr J. Root knot nematodes. CABI Publishing, 301-325.