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Red Lake’ ve ‘Rosenthal’ Frenk Üzüm Çeşitlerinin Polen Performansları

Year 2019, Volume: 56 Issue: 3, 313 - 317, 27.09.2019
https://doi.org/10.20289/zfdergi.506729

Abstract

Amaç : Bu çalışmanın amacı ‘Red Lake’ ve ‘Rosenthal’ frenk üzümü çeşitlerinin polen performanslarının belirlenmesidir. Bu amaçla iki çeşide ait çiçek tozlarında  çiçek tozu morfolojik homojenlik oranı, çiçek tozu canlılık oranı ve farklı sıcaklıklarda çiçek tozu çimlenme oranı araştırılmıştır.

Materyal ve Metod: Çalışmanın bitkisel materyalini ‘Red Lake’ ve ‘Rosenthal’ frenk üzümüne ait çiçek tozları oluşturmaktadır. Döllenme biyolojileri çalışmaları için ilk once fenolojik kayıtlar tutulmuştur. Polen performansını belirlemek amacıyla, morfolojik homojenlik, polen canlılığı (TTC boyama testi) ve çimlenme oranları (petride agar) belirlenmiştir. Ayrıca, farklı sıcaklık koşullarının (15C0, 20C0, 25C0, 30C0) polen çimlenmesi üzerine etkileri yapılmış ve çeşitler için en uygun

çimlenme sıcaklığı belirlenmiştir. Farklı inkübasyon sürelerinin (6 saat, 12 saat, 24 saat, 48 saat) çimlenme kapasitesi üzerindeki etkisi de belirlenmiştir.

Bulgular : Çeşitlerin morfolojik homojenlik değerleri oldukça yüksekti ve morfolojik olarak homojen polenlerden daha yüksek polen canlılığı elde edildi. Her iki çeşidin çimlenme yüzdesine göre farklı sıcaklıkların etkisi istatistiksel olarak farklı bulundu (p <0.05).

Sonuçlar: 15C0, ‘Red Lake’ çeşidi için en uygun çiçlenme sıcaklığı olurken, 20 C0, ‘Rosenthal’ çeşidi için optimum çimlenme sıcaklığı olarak bulunmuştur. İnkubasyon süresinin artmasıyla her iki çeşit içinde çiçek tozu çimlenme oranları artmıştır.

References

  • Brennan, R.M., 2008. Currants and gooseberries. In: Hancock, J.F. (Ed.), Temperate Fruit Crop Breeding: Germplasm to Genomics. Springer, Dordrecht, pp. 177–196.
  • Dafni, A., D. Firmage. 2000. Pollen viability and longevity: practical, ecological and evolutionary implications A. Dafni, M. Hesse, E. Pacini (Eds.), Pollen And Pollination, Springer, Vienna (2000), pp. 113-132.
  • Dane, F., G. Olgun, Ö. Dalgıç. 2004. In vitro pollen germination of some plant species in basic culture medium. J. Cell Mol. Biol., 3:71-76. (in Turkey)
  • Denisow, B. 2002. The influence of the degree of pollination of black currant flowers (Ribes nigrum L.) on the number of seeds in fruits and its size. Annales UMCS section EEE 11: 11–18.
  • Engin, H., Hepaksoy, S. 2003. Bazı Nar Çeşitlerinin Çiçek Tozu Çimlenme Güçlerinin Belirlenmesi Ege Üniv. Ziraat Fak. Derg., 40 (3):9-16
  • Ercisli, S. 2007. Determination of pollen viability and in vitro pollen germination of Rosa dumalis and Rosa villosa. Bangladesh J. Botany., 36:185-187.
  • Gadze, J., M. Radunic, I.V. Petric, S. Ercisli. 2011. In vitro pollen viability, germination
 and pollen tube growth in some pomegranate (Punica granatum l.) cultivars from Croatia and Bosnia and Herzegovina. Acta Sci. Pol., Hortorum Cultus., 10 (3): 297-305
  • Güçlü, S. F., Öncü, Z. and Koyuncu, F. 2015. Bazı Sert Çekirdekli Meyve Türlerinde Çiçek Tozu Çimlenmesi ve Çim Borusu Uzunluğunun Çoklu Regresyon Yöntemi ile Modellenmesi. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 19(3)
  • Hedly A., J.I. Hormaza, M. Herrero. 2004. Effect of temperature on pollen tube kinetics and dynamics in sweet cherry, Prunus avium (Rosaceae). Am. J. Bot., 91: 558–564.
  • Henriques, A. T., V. L. Basan, M.B. Raseira, J.A.S. Zuanazzi. 2004. Antocianos e capacidade antioxidante de frutas. In EMBRAPA (ed). Proceedings of 2° Simposio Nacional do Morango, 1° Encontro de Pequenas Frutas e Frutas Nativas do MERCOSUR, Pelotas, Brasil, pp. 271-280.
  • Johanson, H.M., G.A. Stephenson. 1998. Effect of temperature during microsporogenensis on pollen performance in Cucurbita pepo (Cucurbitacea). Int. J. Plant Sci. 159 (4): 616-626.
  • Khan, S.A., P. Anjum. 2014. In vitro pollen germination of five citrus species. Pak. J. Bot. 46 (3): 951-956.
  • Koyuncu, F. 2006. Response of in vitro pollen tube growth of strawberry cultivars to temperature. Eur. J. Hortic. Sci., 71:125-128.
  • Koyuncu, F., F. Güçlü. 2009. Effect of temperature on in vitro pollen germination and tube growth in sweet cherries. American-Eurasian J. Agric. & Environ. Sci. 6 (5):520-525.
  • Pham, W.T., M. Herrero, J.I. Hormaza. 2015. Effect of temperature on pollen germination and pollen tube growth in longan (Dimocarpus longan Lour.) Sci. Hortic., article in press.
  • Senters, A.E., D.E. Soltis. 2003. Phylogenetic relationships in Ribes (Grossulariaceae) inferred from ITS sequence data. Taxon. 52:51–66.
  • Sharafi, Y., M. Babash Pour, M. Karimi. 2010. In vitro pollen germination and pollen tube growth in some Rosa canina genotypes. Int. Med. Arom. Plants, 29-31.
  • Sinnott, Q. 1985. A revision of Ribes L. subg. Grossularia (Mill.) per. Sect. Grossularia (Mill.) Nutt. (Grossulariaceae) in North America. Rhodora, 87:189-286.
  • Sulusoglu, M., A. Cavusoglu. 2014. In vitro pollen viability and pollen germination in cherry laurel (Prunus laurocerasus L.) The Scientific World Journal, 657123, 7 pages
  • Thompson M. 2004. Flowering, pollination and fruit set. In: Webster A.D., Looney N.E. (eds.), Cherries, Crop Physiology, Production and Uses. Wallingford, CABI Publishing: 223-243.
  • Tosun, F., F. Koyuncu. 2007. Investigations of suitable pollinator for 0900 ziraat sweet cherry cv. pollen performance tests, germination tests, germination procedures, in vitro and in vivo pollinations. Hortic. Sci., 34 (2): 47–53.
  • Vizintin L., B. Bohonec. 2004. In vitro manipulation of cucumber (Cucumis sativus L.) pollen and microspores: isolation procedures, viability tests, germination, maturation. Acta Biol. Cracov. Ser. Bot. 46:177-183.

Pollen Performance of ‘Red Lake’ and ‘Rosenthal’ Currant (Ribes rubrum) Cultivars

Year 2019, Volume: 56 Issue: 3, 313 - 317, 27.09.2019
https://doi.org/10.20289/zfdergi.506729

Abstract

Objective: The aim of this study is to test the performance of the pollen grains of ‘Red Lake’ and ‘Rosenthal’ blackcurrant varieties. Pollen morphological homogeneity ratio, pollen viability ratio and germination rate at different temperatures were investigated.

Material and Methods: In this study, pollen germination, viability and quality of pollens belongs to ‘Red Lake’ and ‘Rosenthal’ currant cultivars were studied. For the fertilization biology studies, phenologic observations were first noted. In order to determine the pollen performance, morphological homogeneity, pollen viability (TTC staining test) and germination rates (agar in plate) were determined. In addition, the effect of different temperature conditions (15 °C, 20 0C, 25 0C, 30 0C) on pollen germination was noted and the most suitable germination temperature was determined for each cultivar. The effect of different incubation times (6h, 12h, 24h, 48h) on germination capacity was also determined.

Results: Morphological homogeneity value of the pollen of the two cultivars was quite high and higher pollen viability should be obtained from morphologically homogenous pollens. Effect of different temperatures on pollen germination percentage was found to be statistically different (p<0.05) for both cultivars.

Conclusions: 150C was the optimum temperature to obtain the highest pollen germination for ‘Red Lake’ while 200C was the optimum for ‘Rosenthal’ pollen grains. Increasing incubation period for both cultivars increased overall pollen germination rate.

References

  • Brennan, R.M., 2008. Currants and gooseberries. In: Hancock, J.F. (Ed.), Temperate Fruit Crop Breeding: Germplasm to Genomics. Springer, Dordrecht, pp. 177–196.
  • Dafni, A., D. Firmage. 2000. Pollen viability and longevity: practical, ecological and evolutionary implications A. Dafni, M. Hesse, E. Pacini (Eds.), Pollen And Pollination, Springer, Vienna (2000), pp. 113-132.
  • Dane, F., G. Olgun, Ö. Dalgıç. 2004. In vitro pollen germination of some plant species in basic culture medium. J. Cell Mol. Biol., 3:71-76. (in Turkey)
  • Denisow, B. 2002. The influence of the degree of pollination of black currant flowers (Ribes nigrum L.) on the number of seeds in fruits and its size. Annales UMCS section EEE 11: 11–18.
  • Engin, H., Hepaksoy, S. 2003. Bazı Nar Çeşitlerinin Çiçek Tozu Çimlenme Güçlerinin Belirlenmesi Ege Üniv. Ziraat Fak. Derg., 40 (3):9-16
  • Ercisli, S. 2007. Determination of pollen viability and in vitro pollen germination of Rosa dumalis and Rosa villosa. Bangladesh J. Botany., 36:185-187.
  • Gadze, J., M. Radunic, I.V. Petric, S. Ercisli. 2011. In vitro pollen viability, germination
 and pollen tube growth in some pomegranate (Punica granatum l.) cultivars from Croatia and Bosnia and Herzegovina. Acta Sci. Pol., Hortorum Cultus., 10 (3): 297-305
  • Güçlü, S. F., Öncü, Z. and Koyuncu, F. 2015. Bazı Sert Çekirdekli Meyve Türlerinde Çiçek Tozu Çimlenmesi ve Çim Borusu Uzunluğunun Çoklu Regresyon Yöntemi ile Modellenmesi. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 19(3)
  • Hedly A., J.I. Hormaza, M. Herrero. 2004. Effect of temperature on pollen tube kinetics and dynamics in sweet cherry, Prunus avium (Rosaceae). Am. J. Bot., 91: 558–564.
  • Henriques, A. T., V. L. Basan, M.B. Raseira, J.A.S. Zuanazzi. 2004. Antocianos e capacidade antioxidante de frutas. In EMBRAPA (ed). Proceedings of 2° Simposio Nacional do Morango, 1° Encontro de Pequenas Frutas e Frutas Nativas do MERCOSUR, Pelotas, Brasil, pp. 271-280.
  • Johanson, H.M., G.A. Stephenson. 1998. Effect of temperature during microsporogenensis on pollen performance in Cucurbita pepo (Cucurbitacea). Int. J. Plant Sci. 159 (4): 616-626.
  • Khan, S.A., P. Anjum. 2014. In vitro pollen germination of five citrus species. Pak. J. Bot. 46 (3): 951-956.
  • Koyuncu, F. 2006. Response of in vitro pollen tube growth of strawberry cultivars to temperature. Eur. J. Hortic. Sci., 71:125-128.
  • Koyuncu, F., F. Güçlü. 2009. Effect of temperature on in vitro pollen germination and tube growth in sweet cherries. American-Eurasian J. Agric. & Environ. Sci. 6 (5):520-525.
  • Pham, W.T., M. Herrero, J.I. Hormaza. 2015. Effect of temperature on pollen germination and pollen tube growth in longan (Dimocarpus longan Lour.) Sci. Hortic., article in press.
  • Senters, A.E., D.E. Soltis. 2003. Phylogenetic relationships in Ribes (Grossulariaceae) inferred from ITS sequence data. Taxon. 52:51–66.
  • Sharafi, Y., M. Babash Pour, M. Karimi. 2010. In vitro pollen germination and pollen tube growth in some Rosa canina genotypes. Int. Med. Arom. Plants, 29-31.
  • Sinnott, Q. 1985. A revision of Ribes L. subg. Grossularia (Mill.) per. Sect. Grossularia (Mill.) Nutt. (Grossulariaceae) in North America. Rhodora, 87:189-286.
  • Sulusoglu, M., A. Cavusoglu. 2014. In vitro pollen viability and pollen germination in cherry laurel (Prunus laurocerasus L.) The Scientific World Journal, 657123, 7 pages
  • Thompson M. 2004. Flowering, pollination and fruit set. In: Webster A.D., Looney N.E. (eds.), Cherries, Crop Physiology, Production and Uses. Wallingford, CABI Publishing: 223-243.
  • Tosun, F., F. Koyuncu. 2007. Investigations of suitable pollinator for 0900 ziraat sweet cherry cv. pollen performance tests, germination tests, germination procedures, in vitro and in vivo pollinations. Hortic. Sci., 34 (2): 47–53.
  • Vizintin L., B. Bohonec. 2004. In vitro manipulation of cucumber (Cucumis sativus L.) pollen and microspores: isolation procedures, viability tests, germination, maturation. Acta Biol. Cracov. Ser. Bot. 46:177-183.
There are 22 citations in total.

Details

Primary Language English
Subjects Engineering
Journal Section Articles
Authors

Sultan Filiz Güçlü 0000-0003-0561-7037

Mehmet Polat

Volkan Okatan This is me 0000-0001-5787-7573

Publication Date September 27, 2019
Submission Date January 2, 2019
Acceptance Date February 25, 2019
Published in Issue Year 2019 Volume: 56 Issue: 3

Cite

APA Güçlü, S. F., Polat, M., & Okatan, V. (2019). Pollen Performance of ‘Red Lake’ and ‘Rosenthal’ Currant (Ribes rubrum) Cultivars. Journal of Agriculture Faculty of Ege University, 56(3), 313-317. https://doi.org/10.20289/zfdergi.506729

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