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Susamda kömür çürüklüğü hastalığı (Macrophomina phaseolina)’nın laboratuvar ve tarla şartlarında karakterizasyonu

Year 2024, Volume: 61 Issue: 2, 151 - 163, 01.07.2024
https://doi.org/10.20289/zfdergi.1432773

Abstract

Amaç: Bu çalışma susam bitkisinde kömür çürüklüğü patojeninin (Macrophomina phaseolina) laboratuvar ve tarla şartlarında karakterize edilmesini amaçlamıştır.
Materyal ve Yöntem: 2017 yılında, M. phaseolina’nın iki izolatı Batı Akdeniz Bölgesi’nde Aksu lokasyonunda hastalık belirtisi gösteren bitkilerden izole edilmiştir. Patojenisite testleri serada Muganlı-57 çeşidi kullanılarak yapılmıştır. İzolatların mikromorfolojik özellikleri (koloni gelişmesi, koloni rengi, miselyum ve mikrosklerot) ve klorat hassasiyetleri laboratuvar koşullarında belirlenmiştir. Sıcaklığın koloni gelişmesi üzerine etkisi ve tarlada kömür çürüklüğü hastalığının belirtileri ayrıca detaylı olarak incelenmiştir.
Araştırma Bulguları: Minimal ortamda izolatların mikrosklerot uzunluğu, mikrosklerot genişliği ve koloni gelişmelerinde önemli (%1) farklılıklar tespit edilmiştir. Her iki izolat klorata hassas olarak karakterize edilmiştir. Minimal ortamda Aksus1 izolatı tüylü bir gelişme deseni gösterirken, Aksus2 izolatı kısıtlanmış bir gelişme deseni göstermiştir. Sıcaklık izolatların koloni gelişmelerini önemli (%1) derecede etkilemiştir. En yüksek koloni gelişmeleri 35°C’ de tespit edilirken, en düşük gelişmeler 40°C’ de bulunmuştur.
Sonuç: Sonuçlar kömür çürüklüğü hastalığının epidemiyolojisinin anlaşılmasına yeni katkılar sunmaktadır. Bildiğimiz kadarıyla, bu Türkiye’nin Batı Akdeniz Bölgesi’nde susam bitkilerinde kömür çürüklüğü hastalığının mikroskobik özelliklerini ve tarlada belirtilerini detaylı olarak karakterize eden ilk çalışmadır.

References

  • Abd, S.W. & H.H. AL-Juboory, 2020. Evaluation of the activity of Bacillus subtilis and actinomyces sp. against Macrophomina phaseolina (TASSI) GOID. causative agent of charcoal rot on sesame. Plant Archives, 20 (1): 2343-2348.
  • Ahmed, H.A.M., A.A. Abdel-Razik, M.H.A. Hassan & S.A. Khaled, 2010. Management of charcoal rot of sesame by seed soaking in medicinal plant extracts and hot water. The Plant Pathology Journal, 26 (4): 372-379.
  • Ahmed, N.R. & M.M.A. Ibrahim, 2020. Influence of intercropping legume fodder crop guar with sesame for reducing charcoal-rot and wilt disease complex concerning to its yield and yield components. Annals of Agricultural Science, Moshtohor, 58 (3): 541-552.
  • Akhtar, K.P., G. Sarwar & H.M.I. Arshad, 2011. Temperature response, pathogenicity, seed infection and mutant evaluation against Macrophomina phaseolina causing charcoal rot disease of sesame. Archives of Phytopathology and Plant Protection, 44 (4): 320-330.
  • Amen, S.G., A.A. El-Sharawy, T.H.A. Hassan & M.Y. Abdalla, 2020. Use of biofumigation for controlling sesame root rot in North Sinai. Asian Journal of Plant Pathology, 14 (1): 21-26.
  • Anonymous, 2023a. Meteoroloji Genel Müdürlüğü. Resmi İstatistikler. (Web page: https://www.mgm.gov.tr/veridegerlendirme/il-ve-ilceler-istatistik.aspx?m= ) (Date accessed: November 2023).
  • Anonymous, 2023b. T.C. Çevre, Şehircilik ve İklim Değişikliği Bakanlığı. (Web page: https://www.mgm.gov.tr/veridegerlendirme/il-ve-ilceler-istatistik.aspx?m=ANTALYA ) (Date accessed: November 2023).
  • Balabaskar, P., P. Renganathan & K. Sanjeevkumar, 2015. Occurrence of sesame root rot disease in Cuddalore district of Tamil Nadu and analysis of the variability in cultural characteristics and pathogenicity among isolates of Macrophomina phaseolina (Tassi) Goid. European Journal of Biotechnology and Bioscience, 3 (8): 55-59.
  • Bashir, M.R., A. Mahmood, M. Sajid, M.A. Zeshan, M. Mohsan, Q.A.T. Khan & F.A. Tahir, 2017. Exploitation of new chemistry fungicides against charcoal rot of sesame caused by Macrophomina phaseolina in Pakistan. Pakistan Journal of Phytopathology, 29 (2): 257-263.
  • Bedawy, I. & M. Moharm, 2019. Reaction and performance of some sesame genotypes for resistance to Macrophomina phaseolina, the incitant of charcoal rot disease. Alexandria Science Exchange Journal, 40 (1): 12-18.
  • Deepthi, P., C.S. Shukla, K.P. Verma & E. Siva Sankar Reddy, 2014. Yield loss assessment and influence of temperature and relative humidity on charcoal rot development in sesame (Sesamum indicum L.). The Bioscan, 9 (1): 193-195.
  • Dhingra, O.D. & J.B. Sinclair, 1978. Biology and Pathology of Macrophomina phaseolina. Viçosa/MG, Brasil. UFV, Imprensa Universitária, 166 pp.
  • Elaigwu, M., H.O.A. Oluma & D.I. Ochokwunu, 2017. In vivo and in vitro activities of some plant extracts on Macrophomina phaseolina (Tassi) Goid- the causal agent of charcoal rot of sesame in Benue State, Nigeria. International Journal of Science and Research Methodology, 5 (4): 23-39.
  • FAO, 2023. FAOSTAT. ( Web page: https://www.fao.org/faostat/en/#data/QCL ) (Date accessed: November, 2023). Farooq, S., A. Mohyo-Ud-Din, S. Naz, M. Siddique & S.N. Khan, 2019. Screening of sesame (Sesamum indicum L.) germplasms for resıstance against charcoal rot disease caused by Macrophomina phaseolina (Tassi) Goid. International Journal of Biology and Biotechnology, 16 (2): 407-410.
  • Gupta, G.K., S.K. Sharma & R. Ramteke, 2012. Biology, epidemiology and management of the pathogenic fungus Macrophomina phaseolina (Tassi) Goid with special reference to charcoal rot of soybean (Glycine max (L.) Merrill). Journal of Phytopathology, 160 (4): 167-180.
  • Gupta, R.N., H.S. Saharan, A.S. Rathi & R. Avtar, 2020. Identification of resistance sources against charcoal rot of sesame incited by Macrophomina phaseolina (Tassi) Goid. International Journal of Current Microbiology and Applied Sciences, 9 (2): 1795-1801.
  • Iqbal, U., T. Mukhtar, S. Muhammad, I.U.H. Iqbal & S.R. Malik, 2010. Host plant resistance in blackgram against charcoal rot (Macrophomina phaseolina (Tassi) Goid). Pakistan Journal of Phytopathology, 22 (2): 126-129.
  • Karibasappa, C.S., B.N. Bhat & S.C. Rao, 2018. Survey for the disease incidence of root rot of sesame caused by Macrophomina phaseolina (Tassi.) Goid, in major sesame growing areas of Telangana. Journal of Pharmacognosy and Phytochemistry, 7 (6): 655-657.
  • Karibasappa, C.S., B.N. Bhat, & S.C. Rao, 2020. Evaluation of fungicides against Macrophomina phaseolina inciting root rot of sesame. International Journal of Current Microbiology and Applied Sciences, 9 (4): 1293-1299.
  • Linhai, W., Z. Yanxin, L. Donghua, H. Junbin, W. Wenliang, L. Haixia & Z. Xiurong, 2011. Variations in the isolates of Macrophomina phaseolina from sesame in China based on amplified fragment length polymorphism (AFLP) and pathogenicity. African Journal of Microbiology Research, 5 (31): 5584-5590.
  • Manici, L.M., F. Caputo & C. Cerato, 1995. Temperature responses of isolates of Macrophomina phaseolina from different climate regions of sunflower production in Italy. Plant Disease, 79 (8): 834-838.
  • Mihail, J.D. & S.J. Taylor, 1995. Interpreting variability among isolates of Macrophomina phaseolina in pathogenicity, pycnidium production, and chlorate utilization. Canadian Journal of Botany, 73 (10): 1596-1603.
  • Min, Y.Y. & K. Toyota, 2019. Occurrence of different kinds of diseases in sesame cultivation in Myanmar and their impact to sesame yield. Journal of Experimental Agriculture International, 38 (4): 1-9.
  • Nayar, N.M. & K.L. Mehra, 1970. Sesame: its uses, botany, cytogenetics, and origin. Economic Botany, 24 (1): 20-31.
  • Omar, M.R., K.A. Abd-Elsalam, A.A. Aly, A.M.A. El-Samawaty & J.-A. Verreet, 2007. Diversity of Macrophomina phaseolina from cotton in Egypt: Analysis of pathogenicity, chlorate phenotypes, and molecular characterization. Journal of Plant Diseases and Protection, 114 (5): 196-204.
  • Pearson, C.A.S., J.F. Leslie & F.W. Schwenk, 1986. Variable chlorate resistance in Macrophomina phaseolina from corn, soybean and soil. Phytopathology, 76 (6): 646-649.
  • Radhakrishnan, R., R. Sathasivam, R.L. Rengarajan, A. Hashem & E.F. Abd_Allah, 2017. Isolation and identification of charcoal rot disease causing agent in sesame (Sesamum indicum L.) and their growth inhibition by bacillus methylotrophicus KE2. Pakistan Journal of Botany, 49 (6): 2495-2497.
  • Ram, R., D. Catlin, J. Romero & C. Cowley, 1990. "Sesame: New Approaches for Crop Improvement, 225-228 ". In: Advances in New Crops (Eds. J. Janick & J.E. Simon) Timber Press, Portland, OR, 560 pp.
  • Salahlou, R., N. Safaie & M. Shams-Bakhsh, 2016. Genetic diversity of Macrophomina phaseolina populations, the causal agent of sesame charcoal rot using inter-simple sequence repeat markers. Journal of Agricultural Science and Technology, 18 (1): 277-287.
  • Sarkar, T.S., P. Biswas, S.K. Ghosh & S. Ghosh, 2014. Nitric oxide production by necrotrophic pathogen Macrophomina phaseolina and the host plant in charcoal rot disease of jute: Complexity of the interplay between necrotroph-host plant interactions. Plos One, 9: e107348.
  • Sarr, M.P., M. Ndiaye, J. Groenewald & P. Crous, 2014. Genetic diversity in Macrophomina phaseolina, the causal agent of charcoal rot. Phytopathologia Mediterranea, 53 (2): 250-268.
  • Savaliya, V.A., C.M. Bhaliya, P.B. Marviyaand & L.F. Akbari, 2015. Evaluation of phytoextracts against Macrophomina phaseolina (Tassi) Goid causing root rot of sesame. JBiopest, 8 (2): 116-119.
  • Shekhar, M., R.C. Sharma, L. Singh & R. Dutta, 2006. Morphological and pathogenic variability of Macrophomina phaseolina (Tassi.) Goid. incitant of charcoal rot of maize in India. Indian Phytopathology, 59 (3): 294-298.
  • Siddiq, J.A., K. Kalpana, E.G. Ebenezar & C. Chinniah, 2019. In vitro efficacy of soluble silicon against sesame (Sesamum indicum L.) charcoal rot disease caused by Macrophomina phaseolina (Tassi) Goid. Journal of Pharmacognosy and Phytochemistry, 8 (3): 3532-3536.
  • Vinothini, K., P. Renganathan, P. Balabaskar & T. Sivakumar, 2020. Occurrence of root rot incidence of sesame and assessment of cultural and pathogenic variability among isolates of Macrophomina phaseolina. Plant Archives, 20 (1): 1923-1928.
  • White, T.J., T. Bruns, S. Lee & J.W. Taylor, 1990. "Amplification and Direct Sequencing of Fungal Ribosomal RNA Genes for Phylogenetics, 315-322". In: PCR Protocols: A Guide to Methods and Applications (Eds. M.A. Innis & D.H Gelfand), Academic Press, Inc. New York, 482 pp.
  • Yılmaz, M., 2022. Bazı susam (Sesamum indicum L.) genotiplerinin Doğu Akdeniz geçit koşullarında verim ve verim unsurlarının belirlenmesi. Ege Üniversitesi Ziraat Fakültesi Dergisi, 59 (4): 708-714.

In vitro and in vivo characterization of charcoal rot disease (Macrophomina phaseolina) in sesame

Year 2024, Volume: 61 Issue: 2, 151 - 163, 01.07.2024
https://doi.org/10.20289/zfdergi.1432773

Abstract

Objective: This study aimed to characterize charcoal rot pathogen (Macrophomina phaseolina) of sesame in vitro and in vivo.
Material and Methods: In 2017, two isolates of M. phaseolina were isolated from symptomatic sesame plants in Aksu district of the Western Mediterranean region of Türkiye. Pathogenicity tests were conducted using cv. Muganlı-57 in a greenhouse. Micromorphological characteristics (colony growth, colony color, mycelium, and microsclerotium) and chlorate sensitivity of the isolates were determined in vitro. Effects of temperature on colony growth and symptoms of charcoal rot in the field were also examined in detail.
Results: Significant (P<0.01) differences were detected in microsclerotium length, microsclerotium width, and colony growths of the isolates on minimal medium. Both isolates were characterized as chlorate-sensitive. On minimal medium, Aksus1 isolate displayed a feathery growth pattern, while Aksus2 isolate showed a restricted growth pattern. Temperature significantly (P˂0.01) affected colony growth. The highest colony growths were detected at 35°C, while the lowest ones were found at 40°C.
Conclusion: The results may provide new insights into the epidemiology of the charcoal rot disease. To the best of our knowledge, this is the first detailed characterization of microscopic traits and charcoal rot symptoms on sesame in the field in the Western Mediterranean region of Türkiye.

References

  • Abd, S.W. & H.H. AL-Juboory, 2020. Evaluation of the activity of Bacillus subtilis and actinomyces sp. against Macrophomina phaseolina (TASSI) GOID. causative agent of charcoal rot on sesame. Plant Archives, 20 (1): 2343-2348.
  • Ahmed, H.A.M., A.A. Abdel-Razik, M.H.A. Hassan & S.A. Khaled, 2010. Management of charcoal rot of sesame by seed soaking in medicinal plant extracts and hot water. The Plant Pathology Journal, 26 (4): 372-379.
  • Ahmed, N.R. & M.M.A. Ibrahim, 2020. Influence of intercropping legume fodder crop guar with sesame for reducing charcoal-rot and wilt disease complex concerning to its yield and yield components. Annals of Agricultural Science, Moshtohor, 58 (3): 541-552.
  • Akhtar, K.P., G. Sarwar & H.M.I. Arshad, 2011. Temperature response, pathogenicity, seed infection and mutant evaluation against Macrophomina phaseolina causing charcoal rot disease of sesame. Archives of Phytopathology and Plant Protection, 44 (4): 320-330.
  • Amen, S.G., A.A. El-Sharawy, T.H.A. Hassan & M.Y. Abdalla, 2020. Use of biofumigation for controlling sesame root rot in North Sinai. Asian Journal of Plant Pathology, 14 (1): 21-26.
  • Anonymous, 2023a. Meteoroloji Genel Müdürlüğü. Resmi İstatistikler. (Web page: https://www.mgm.gov.tr/veridegerlendirme/il-ve-ilceler-istatistik.aspx?m= ) (Date accessed: November 2023).
  • Anonymous, 2023b. T.C. Çevre, Şehircilik ve İklim Değişikliği Bakanlığı. (Web page: https://www.mgm.gov.tr/veridegerlendirme/il-ve-ilceler-istatistik.aspx?m=ANTALYA ) (Date accessed: November 2023).
  • Balabaskar, P., P. Renganathan & K. Sanjeevkumar, 2015. Occurrence of sesame root rot disease in Cuddalore district of Tamil Nadu and analysis of the variability in cultural characteristics and pathogenicity among isolates of Macrophomina phaseolina (Tassi) Goid. European Journal of Biotechnology and Bioscience, 3 (8): 55-59.
  • Bashir, M.R., A. Mahmood, M. Sajid, M.A. Zeshan, M. Mohsan, Q.A.T. Khan & F.A. Tahir, 2017. Exploitation of new chemistry fungicides against charcoal rot of sesame caused by Macrophomina phaseolina in Pakistan. Pakistan Journal of Phytopathology, 29 (2): 257-263.
  • Bedawy, I. & M. Moharm, 2019. Reaction and performance of some sesame genotypes for resistance to Macrophomina phaseolina, the incitant of charcoal rot disease. Alexandria Science Exchange Journal, 40 (1): 12-18.
  • Deepthi, P., C.S. Shukla, K.P. Verma & E. Siva Sankar Reddy, 2014. Yield loss assessment and influence of temperature and relative humidity on charcoal rot development in sesame (Sesamum indicum L.). The Bioscan, 9 (1): 193-195.
  • Dhingra, O.D. & J.B. Sinclair, 1978. Biology and Pathology of Macrophomina phaseolina. Viçosa/MG, Brasil. UFV, Imprensa Universitária, 166 pp.
  • Elaigwu, M., H.O.A. Oluma & D.I. Ochokwunu, 2017. In vivo and in vitro activities of some plant extracts on Macrophomina phaseolina (Tassi) Goid- the causal agent of charcoal rot of sesame in Benue State, Nigeria. International Journal of Science and Research Methodology, 5 (4): 23-39.
  • FAO, 2023. FAOSTAT. ( Web page: https://www.fao.org/faostat/en/#data/QCL ) (Date accessed: November, 2023). Farooq, S., A. Mohyo-Ud-Din, S. Naz, M. Siddique & S.N. Khan, 2019. Screening of sesame (Sesamum indicum L.) germplasms for resıstance against charcoal rot disease caused by Macrophomina phaseolina (Tassi) Goid. International Journal of Biology and Biotechnology, 16 (2): 407-410.
  • Gupta, G.K., S.K. Sharma & R. Ramteke, 2012. Biology, epidemiology and management of the pathogenic fungus Macrophomina phaseolina (Tassi) Goid with special reference to charcoal rot of soybean (Glycine max (L.) Merrill). Journal of Phytopathology, 160 (4): 167-180.
  • Gupta, R.N., H.S. Saharan, A.S. Rathi & R. Avtar, 2020. Identification of resistance sources against charcoal rot of sesame incited by Macrophomina phaseolina (Tassi) Goid. International Journal of Current Microbiology and Applied Sciences, 9 (2): 1795-1801.
  • Iqbal, U., T. Mukhtar, S. Muhammad, I.U.H. Iqbal & S.R. Malik, 2010. Host plant resistance in blackgram against charcoal rot (Macrophomina phaseolina (Tassi) Goid). Pakistan Journal of Phytopathology, 22 (2): 126-129.
  • Karibasappa, C.S., B.N. Bhat & S.C. Rao, 2018. Survey for the disease incidence of root rot of sesame caused by Macrophomina phaseolina (Tassi.) Goid, in major sesame growing areas of Telangana. Journal of Pharmacognosy and Phytochemistry, 7 (6): 655-657.
  • Karibasappa, C.S., B.N. Bhat, & S.C. Rao, 2020. Evaluation of fungicides against Macrophomina phaseolina inciting root rot of sesame. International Journal of Current Microbiology and Applied Sciences, 9 (4): 1293-1299.
  • Linhai, W., Z. Yanxin, L. Donghua, H. Junbin, W. Wenliang, L. Haixia & Z. Xiurong, 2011. Variations in the isolates of Macrophomina phaseolina from sesame in China based on amplified fragment length polymorphism (AFLP) and pathogenicity. African Journal of Microbiology Research, 5 (31): 5584-5590.
  • Manici, L.M., F. Caputo & C. Cerato, 1995. Temperature responses of isolates of Macrophomina phaseolina from different climate regions of sunflower production in Italy. Plant Disease, 79 (8): 834-838.
  • Mihail, J.D. & S.J. Taylor, 1995. Interpreting variability among isolates of Macrophomina phaseolina in pathogenicity, pycnidium production, and chlorate utilization. Canadian Journal of Botany, 73 (10): 1596-1603.
  • Min, Y.Y. & K. Toyota, 2019. Occurrence of different kinds of diseases in sesame cultivation in Myanmar and their impact to sesame yield. Journal of Experimental Agriculture International, 38 (4): 1-9.
  • Nayar, N.M. & K.L. Mehra, 1970. Sesame: its uses, botany, cytogenetics, and origin. Economic Botany, 24 (1): 20-31.
  • Omar, M.R., K.A. Abd-Elsalam, A.A. Aly, A.M.A. El-Samawaty & J.-A. Verreet, 2007. Diversity of Macrophomina phaseolina from cotton in Egypt: Analysis of pathogenicity, chlorate phenotypes, and molecular characterization. Journal of Plant Diseases and Protection, 114 (5): 196-204.
  • Pearson, C.A.S., J.F. Leslie & F.W. Schwenk, 1986. Variable chlorate resistance in Macrophomina phaseolina from corn, soybean and soil. Phytopathology, 76 (6): 646-649.
  • Radhakrishnan, R., R. Sathasivam, R.L. Rengarajan, A. Hashem & E.F. Abd_Allah, 2017. Isolation and identification of charcoal rot disease causing agent in sesame (Sesamum indicum L.) and their growth inhibition by bacillus methylotrophicus KE2. Pakistan Journal of Botany, 49 (6): 2495-2497.
  • Ram, R., D. Catlin, J. Romero & C. Cowley, 1990. "Sesame: New Approaches for Crop Improvement, 225-228 ". In: Advances in New Crops (Eds. J. Janick & J.E. Simon) Timber Press, Portland, OR, 560 pp.
  • Salahlou, R., N. Safaie & M. Shams-Bakhsh, 2016. Genetic diversity of Macrophomina phaseolina populations, the causal agent of sesame charcoal rot using inter-simple sequence repeat markers. Journal of Agricultural Science and Technology, 18 (1): 277-287.
  • Sarkar, T.S., P. Biswas, S.K. Ghosh & S. Ghosh, 2014. Nitric oxide production by necrotrophic pathogen Macrophomina phaseolina and the host plant in charcoal rot disease of jute: Complexity of the interplay between necrotroph-host plant interactions. Plos One, 9: e107348.
  • Sarr, M.P., M. Ndiaye, J. Groenewald & P. Crous, 2014. Genetic diversity in Macrophomina phaseolina, the causal agent of charcoal rot. Phytopathologia Mediterranea, 53 (2): 250-268.
  • Savaliya, V.A., C.M. Bhaliya, P.B. Marviyaand & L.F. Akbari, 2015. Evaluation of phytoextracts against Macrophomina phaseolina (Tassi) Goid causing root rot of sesame. JBiopest, 8 (2): 116-119.
  • Shekhar, M., R.C. Sharma, L. Singh & R. Dutta, 2006. Morphological and pathogenic variability of Macrophomina phaseolina (Tassi.) Goid. incitant of charcoal rot of maize in India. Indian Phytopathology, 59 (3): 294-298.
  • Siddiq, J.A., K. Kalpana, E.G. Ebenezar & C. Chinniah, 2019. In vitro efficacy of soluble silicon against sesame (Sesamum indicum L.) charcoal rot disease caused by Macrophomina phaseolina (Tassi) Goid. Journal of Pharmacognosy and Phytochemistry, 8 (3): 3532-3536.
  • Vinothini, K., P. Renganathan, P. Balabaskar & T. Sivakumar, 2020. Occurrence of root rot incidence of sesame and assessment of cultural and pathogenic variability among isolates of Macrophomina phaseolina. Plant Archives, 20 (1): 1923-1928.
  • White, T.J., T. Bruns, S. Lee & J.W. Taylor, 1990. "Amplification and Direct Sequencing of Fungal Ribosomal RNA Genes for Phylogenetics, 315-322". In: PCR Protocols: A Guide to Methods and Applications (Eds. M.A. Innis & D.H Gelfand), Academic Press, Inc. New York, 482 pp.
  • Yılmaz, M., 2022. Bazı susam (Sesamum indicum L.) genotiplerinin Doğu Akdeniz geçit koşullarında verim ve verim unsurlarının belirlenmesi. Ege Üniversitesi Ziraat Fakültesi Dergisi, 59 (4): 708-714.
There are 37 citations in total.

Details

Primary Language English
Subjects Phytopathology
Journal Section Articles
Authors

Mehmet Aydoğdu 0000-0001-5267-4654

İlker Kurbetli 0000-0001-8991-4412

Early Pub Date July 1, 2024
Publication Date July 1, 2024
Submission Date February 6, 2024
Acceptance Date April 23, 2024
Published in Issue Year 2024 Volume: 61 Issue: 2

Cite

APA Aydoğdu, M., & Kurbetli, İ. (2024). In vitro and in vivo characterization of charcoal rot disease (Macrophomina phaseolina) in sesame. Journal of Agriculture Faculty of Ege University, 61(2), 151-163. https://doi.org/10.20289/zfdergi.1432773

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