Farklı konsantrasyonlardaki büyüme düzenleyicilerin patatesin (Solanum tuberosum L.) in vitro çoğalması üzerine etkileri

Year 2025, Volume: 62 Issue: 3, 343 - 354, 18.09.2025

Anna Satyana Karyawati , Rizki Nurachma Maulidi

https://doi.org/10.20289/zfdergi.1539199

Abstract

Amaç: Bu araştırma, kinetin ve NAA konsantrasyon etkileşiminin in vitro patates (Solanum tuberosum L.) fidelerinin büyümesi ve çoğalması üzerindeki etkisini incelemeyi amaçlamaktadır.
Materyal ve Yöntem: Bu araştırma, Aralık 2023 - Mart 2024 tarihleri arasında Brawijaya Üniversitesi, Ziraat Fakültesi, Doku Kültürü Laboratuvarında yürütülmüştür. Araştırma, iki faktörlü tesadüfi grup tasarımında yürütülmüştür. İlk faktör, 0 ppm, 0.25 ppm, 0.50 ppm ve 0.75 ppm olmak üzere 4 konsantrasyon seviyesine sahip Naftalin Asetik Asit (NAA) olmuştur. İkinci faktör ise 0 ppm, 2.5 ppm, 5 ppm ve 7.5 ppm olmak üzere 4 konsantrasyon seviyesine sahip Kinetin'dir.
Araştırma Bulguları: Sürgün sayısı, yaprak sayısı, kök sayısı, bitki boyu ve sürgün çıkış zamanı değişkenlerinde bir etkileşim gözlenmiştir. Sürgün sayısı ve yaprak sayısı, patates fidelerinin büyümesiyle doğrusal veya doğru orantılı sonuçlara sahiptir. En yüksek seviyede 7,5 ppm Kinetin ve 0,75 ppm NAA ile kinetin ve NAA'nın eklenmesi, tüm gözlem değişkenlerindeki sonuçları azaltabilir.
Sonuç: Kinetin 7,5 ppm ve NAA 0,25 ilavesi etkileşime girmiş ve kinetin ilavesiz ve NAA ilavesiz durumlara kıyasla patates fidelerinin sürgün sayısında (%0,46), yaprak sayısında (%0,27) ve kök sayısında (%0,43) daha yüksek büyüme sağlamıştır.

Keywords

Büyüme düzenleyiciler , in vitro , kinetin , çoğaltma , Naftalin Asetik Asit (NAA) , patates fideleri

Project Number

01

The effects of different concentrations of growth regulators on the in vitro multiplication of potato (Solanum tuberosum L.)

Abstract

Objective: This research aims to study the effect of kinetin and NAA concentration interaction on the growth and multiplication of potato planlets (Solanum tuberosum L.) in vitro.
Material and Methods: This research was conducted at the Tissue Culture Laboratory, Faculty of Agriculture, Brawijaya University from December 2023 to March 2024. The research was conducted in a randomized group design with two factors. The first factor is the GR Naphthalene Acetic Acid (NAA) with 4 concentration levels, 0 ppm, 0.25 ppm, 0.50 ppm and 0.75 ppm. The second factor is Kinetin with 4 concentration levels, 0 ppm, 2.5 ppm, 5 ppm and 7.5 ppm.
Results: There is an interaction in the variable number of shoots, number of leaves, number of roots, planlet height and shoot emergence time. The number of shoots and the number of leaves have linear results or directly proportional to the growth of potato planlets. The addition of kinetin and NAA with the highest level of 7.5 ppm Kinetin and 0.75 ppm NAA can reduce the results on all observation variables.
Conclusion The addition of Kinetin 7.5 ppm and NAA 0.25 ppm interacted and produced higher growth in number of shoots (0.46%), number of leaves (0.27%) and number of roots of potato planlets (0.43%) compared to without the addition of kinetin and without the addition of NAA.

Keywords

Growth regulators , in vitro , kinetin , multiplication , Naphthalene Acetic Acid (NAA) , potato planlets

Ethical Statement

Hereby declare that it is true that we have conducted research and made the journal.

Supporting Institution

University of Brawijaya

Project Number

01

Thanks

Thankyou for your attention and contribution

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