The Effects of Cold Stress, Adrenomedullin and Methylatedadrenomedullin Treatments on Total RNA Levels of Some Rat Tissues

Abstract

In this study it was aimed to investigate the effects of cold stress, adrenomedullin and methylated adrenomedullin treatments on total RNA levels of some rat tissues. Total RNA levels were determinated for being able to get introductory information about the enzyme levels which play a central role in control and secretion of stress hormons. During experimental process in cold stress treatment, rats were exposed +10 °C temparature for a week. In AdM and met-AdM treatment groups, animals received intraperitoneal (i.p) injection of AdM and met-AdM (2000ng/kg body weight) once a day during a week. Total RNA anyalysis was determinated according to Tümer et.al. (1997). Statical analysis were performed using SPSS version 12.0. Results were shown as mean± standart deviation. In cold stress treatment groups total RNA levels were decreased in all tissue compared to controls. The differences of between control and cold stress treatment groups were significant (p

Keywords

• Adrenomedullin,methylated-adrenomedullin,cold stress,total RNA,sıçan

SOĞUK STRESİ, ADRENOMEDULLİN ve METİLE ADRENOMEDULLİN UYGULAMALARININ BAZI SIÇAN DOKULARINDA TOPLAM RNA MİKTARLARI ÜZERİNE ETKİLERİ

Öz

Bu çalışmada bazı sıçan dokularında soğuk stresi, adrenomedullin (AdM) ve metile-adrenomedullin (met-AdM) uygulamalarının total RNA miktarları üzerine etkilerinin araştırılması amaçlanmış olup, stres hormonlarının kontrolünde ve salınmasında etkili enzimler ve bunların regülasyonu hakkında önbilgi verebileceği düşüncesi ile total RNA miktarları saptanmıştır. Deney esnasında soğuk stresi uygulama grubunda sıçanlar bir hafta boyunca + 10°C sıcaklığa maruz bırakılmışlar ve AdM ve met- AdM uygulamaları 2000 ng/kg olarak i.p olarak günde bir kez olmak üzere 1 hafta süre ile yapılmıştır. Toplam RNA analizinde ise Tümer ve ark., (1997) tarafından kullanılan metottan yararlanılmıştır. İstatistiksel değerlendirmeler SPSS for Windows Version 12.0 paket program ile yapılmış olup, sonuçlar ortalama ± standart hata ile verilmiştir. Soğuk stresi uygulama gruplarında total RNA seviyeleri tüm dokularda kontrolle kıyaslandığında azalmıştır. Kontrol ile soğuk stresi uygulama grubu arasındaki fark önemli bulunmuştur (p

Anahtar Kelimeler

• Adrenomedullin,metile-adrenomedullin,soğuk stresi,total RNA,sıçan

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