The Effects of Cold Stress, Adrenomedullin and Methylatedadrenomedullin Treatments on Total RNA Levels of Some Rat Tissues

Year 2009, Issue: 13, 183 - 192, 01.12.2009

Nuran Cikcikoğlu Yıldırım Muhittin Yürekli Numan Yıldırım

Abstract

In this study it was aimed to investigate the effects of cold stress, adrenomedullin and methylated adrenomedullin treatments on total RNA levels of some rat tissues. Total RNA levels were determinated for being able to get introductory information about the enzyme levels which play a central role in control and secretion of stress hormons. During experimental process in cold stress treatment, rats were exposed +10 °C temparature for a week. In AdM and met-AdM treatment groups, animals received intraperitoneal (i.p) injection of AdM and met-AdM (2000ng/kg body weight) once a day during a week. Total RNA anyalysis was determinated according to Tümer et.al. (1997). Statical analysis were performed using SPSS version 12.0. Results were shown as mean± standart deviation. In cold stress treatment groups total RNA levels were decreased in all tissue compared to controls. The differences of between control and cold stress treatment groups were significant (p

Keywords

Adrenomedullin, methylated-adrenomedullin, cold stress, total RNA, sıçan

SOĞUK STRESİ, ADRENOMEDULLİN ve METİLE ADRENOMEDULLİN UYGULAMALARININ BAZI SIÇAN DOKULARINDA TOPLAM RNA MİKTARLARI ÜZERİNE ETKİLERİ

Abstract

Bu çalışmada bazı sıçan dokularında soğuk stresi, adrenomedullin (AdM) ve metile-adrenomedullin (met-AdM) uygulamalarının total RNA miktarları üzerine etkilerinin araştırılması amaçlanmış olup, stres hormonlarının kontrolünde ve salınmasında etkili enzimler ve bunların regülasyonu hakkında önbilgi verebileceği düşüncesi ile total RNA miktarları saptanmıştır. Deney esnasında soğuk stresi uygulama grubunda sıçanlar bir hafta boyunca + 10°C sıcaklığa maruz bırakılmışlar ve AdM ve met- AdM uygulamaları 2000 ng/kg olarak i.p olarak günde bir kez olmak üzere 1 hafta süre ile yapılmıştır. Toplam RNA analizinde ise Tümer ve ark., (1997) tarafından kullanılan metottan yararlanılmıştır. İstatistiksel değerlendirmeler SPSS for Windows Version 12.0 paket program ile yapılmış olup, sonuçlar ortalama ± standart hata ile verilmiştir. Soğuk stresi uygulama gruplarında total RNA seviyeleri tüm dokularda kontrolle kıyaslandığında azalmıştır. Kontrol ile soğuk stresi uygulama grubu arasındaki fark önemli bulunmuştur (p

Keywords

Adrenomedullin, metile-adrenomedullin, soğuk stresi, total RNA, sıçan

References

• Axelrod, J. Mueller, R.A. Henry, J.P. (1970). Changes in enzymes involved in the biosynthesis and metabolism of noradrenaline and adrenaline after psychosocial stimulation. Nature; 225, 1059-60.
• Balat, A. Çekmen, M. Yürekli, M. Gülcan, H. Kutlu, O. Türköz, Y. ve Yoloğlu, S. (2000a).Adrenomedullin and nitrite levels in children with minimal change nephrotic syndrome,Pediatric Nephrology,15, 70-73.
• Balat, A. Çekmen, M. Yürekli, M. Kutlu, O. İslek, İ. Sönmezgöz, E. Çakır, M. Türköz, Y.and Yoloğlu, S. (2000b). Adrenomedullin and nitrite levels in children with Bartter syndrome, Pediatric Nephrology, 15, 266-270.
• Bunton, D.C. Petrie M.C. Hillier, C. Johnstone, F. McMurray, J.V. (2004). The Clinical Revelence of Adrenomedullin: a promising profile, Pharmocology& Therapeutics, 103(3), 179-201.
• Clarke, S. (2003). Aging as war between chemical and biochemical processes: protein methylation and the recognition of age-damaged proteins for repair.Ageing Research Reviews, 2(3), 263-285.
• Chen, Y.F. Zhang, A.Y. Zou, A.P. Campbell, W.B. Li, P.L. (2004). Protein methylation activates reconstituted ryanodine receptor-ca release channels from coronary artery myocytes.Journal of Vascular Research, 41(3), 229-240.
• Cimato, T.R. Ettinger, M.J. Zhou, X. Aletta, J.M. (1997). Nerve growth factor-specific regulation of protein methylation during neuronal differentiation of PC12 cells. Journal of Cell Biology, 138(5), 1089-1103.
• Devlin, T.M. (1986). Teztbook of Biochemistry. Hahnemann Univercity School of Medicine A. Wiley Medical Publication. New York,.16.
• Hinson, J.P. Kapas, S. Smith, D.M. (2000). Adrenomedullin, a multifiınctional regulatory peptide. Endocr Rev, 21, 138-67.
• Kujubu, D.A. Stimmel, J.B. Law, R.E. Herschman, H.R. Clarke, S. (1993). Early responses of PC-12 cells to NGF and EGF: effect of K252a and 5'-methylthioadenosine on gene expression and membrane protein methylation. Journal of Neuroscience Research, 36(1), 58-65.
• Liu, X. Kvetnansky, R. Serova, L. Sollas, A. Saban E.L. (2005). Increased susceptibility to transcriptional changes with novel stressor in adrenal medulla of rats exposed to prolonged cold stres. Molecular Brain Research, 141, 19-29.
• Michael, W. K. (2008). IU School of Medicine http://themedicalbiochemistrypage.org/ adresinden 21 ekim 2008 tarihinde indirilmiştir.
• Nikitenko, L.L. Smith, D.M. Hague, S. Wilson, C.R. Bicknell, R. ve Rees, M.C.P. (2002). Adrenomedullin and The Microvasculature. Trends in Pharmacological Sciences, 23(3), 101-103.
• Noyan, A, ,(1980). Fizyoloji ders Kitabı,8. baskı, Ankara: Anadolu Üniversitesi Yayınları. No: 2 Meteksan.
• Roberts, J & Tumer, N. (1987). Age related changes in autonomic function of catecholamines. Rewiew of Biological in Aging; 3, 257-98.
• Selman, C. McLaren.S.J. Hımanka J.M.. ve Speakman R.J. (2000). Effect of Long –Term Cold Exposure on Antıoxidant Enzyme Actıvıtıes İn Small Mammal, Free Radical Biology&Medicine, 28(8), 1279-1285.
• Samson, W. K. (1999). Adrenomedullin and the control of fluid and electrolyte homeostasis, Annual Review of Physiology, 61, 363-389.
• Şahin, E. & Gümüşlü, S. (2004). Cold-stress-induced modulation of antioxidant defence: role of stressed conditions in tissue injury followed by protein oxidation and lipid peroxidation, Int J.Biometerol, 48, 165-171.
• Tümer, N. La Rochelle, J.S. and Yürekli, M. (1997). Exercise training reverses the age-related decline in tyrosine hydroxylase expression in rat hypothalamus. Journal of Gerontology: Biological Science, 52A (5), 255-259.
• Vemuri, R. & Philipson, K.D. (1988). Protein methylation inhibits Na+-Ca2+ exchange activity in cardiac sarcolemmal vesicles.Biochimica et Biophysica Acta, 939(3), 503-508.
• Yüksel, Ş. Akbay, A. & Yürekli, M. (2002). Contibution of Adrenomedullin to homeostatic response to cold Stress in rat model, Pathophysiology 8, 243-247.
• Yuksel, Ş. Asma, D. (2006). Effects of extended cold exposure on antioxidant defense system of rat hypothalamic–pituitary–adrenal axis. Journal of Thermal Biology 31, 313–317.
• Zudaire, E. Martínez, A. Cuttitta, F. (2003). Adrenomedullin and cancer,Regul Pept. Apr 15;112(1-3), 175-83.