Yüksek fruktoz içerikli beslenmenin overlerde perilipin ekspresyonu ve polikistik over gelişimi ile ilişkisi

Year 2016, , 106 - 111, 08.11.2016

İlknur Keskin , Nejda Bedri

https://doi.org/10.16948/zktipb.257211

Abstract

Amaç: PCOS ve yüksek fruktoz içerikli diyet
birlikteliğini gösteren çalışmalar kısıtlı sayıdadır. Çalışmamızda ratlarda
yüksek fruktoz içerikli beslenmenin overlerde perilipin ekspresyonu üzerine
etkisi ve Polikistik Over gelişimi ile ilgisini ortaya koymayı amaçladık.

Gereç ve Yöntem: Çalışmamızda hiçbir uygulama
yapılmayan Kontrol (K) grubu, Yüksek Fruktoz (HF) grubu ve DHEA
(dehidroepiandrosteron) enjekte edilen Hormon (H) grubu over yapıları
karşılaştırıldı. Gruplar arasındaki morfolojik farklılıkları gösterebilmek için
hematoksilen-eozin boyaması yapıldı, PLIN2 ve PLIN3 antikorları için western
blot yöntemi uygulandı. Progesteron ve LH seviyeleri ELISA tekniği ile ölçüldü.

Bulgular: HF ve Hormon gruplarında antral ve
preantral folikül sayısının artmış olduğu gözlendi. PLIN2 ve PLIN3
ekspresyonlarının Hormon ve HF gruplarında yüksek olduğu görüldü. Progesteron
ve LH seviyelerinde anlamlı bir farklılık saptanmadı.

Sonuç: Yüksek fruktozlu diyet ile beslenme
PCOS oluşumuna neden olabilir ve bu mekanizmada PLIN2 ve PLIN3 proteinlerinin
aşırı ekspresyonu etkili olabilir.

Keywords

fare, fruktoz, ovaryum, PCOS

Relationship between maintaning diet containing high fructose and development of polycystic ovary via perilipin expression

Abstract

Introduction: Diet comes at the first place
among factors affecting PCOS development. Studies showing diet consisting of
PCOS combined with high fructose are in a limited level. In this study, it is
aimed to investigate PLIN2 and PLIN3 expressions by showing diet consisting of
combination of high fructose and PCOS in Juvenile phase.

Material-Methods: A total of 26 Balb/C
type female mice have been used and 3 groups are created: Control (no
treatment), HF (tap water containing 20% fructose is given by oral gavege) and Hormone
(The injection of DHEA  is injected s.c. for
20 days). In order to show the morphological differences between the groups haematoxylin and eosin staining method has been used. For PLIN2 and PLIN3 antibodies western blot method
has been used. ELISA
technic has been used to compare progesterone and LH levels between the groups.

Results: It is seen that antral and preantral follicle numbers of HF and Hormone
groups have increased. It is also seen that PLIN2 and PLIN3 expressions are
high in HF and Hormone groups. As a result diet with high fructose could
contribute to PCOS development and in this mechanism PLIN2 and PLIN3 proteins
could also be effective. 

Keywords

mice, fructose, ovary, PCOS

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