İn vitro fertilizasyon olgularında serım ve folliküler sıvı total oksidan ve antioksidan seviyelerinin incelenmesi

Year 2018, , 136 - 139, 04.04.2018

Emel Ozturk Tufan Oge , Yunus Aydin , Ozben Isiklar Hikmet Hassa

https://doi.org/10.16948/zktipb.332539

Cited By: 1

Abstract

Amaç: İnfertilite teşhisi konmuş hastaların İn Vitro Fertilizasyon (IVF) uygulamalarında
serum ve folliküler sıvılarında total oksidan kapasite (TOC) ,total antioksidan
kapasite (TAC) ve oksidatif stres indeksi (OSI) değerlerinin oosit
gelişimi,fertilizasyon, embriogenezis ve klinik gebelik üzerine etkilerini
değerlendirmek.

Materyal Metod: Kliniğimizde IVF
protokolüne alınan 100 hastanın çalışma grubunu oluşturdu ve gonodatropın
başlandığı gün, embriyo transferi günü ve oosit toplandığı gün kan örnekleri
alındı. İlave olarak oosit toplandığı sırada folliküler sıvı da toplandı.  Serumda ve folliküler sıvıda TOC , TAC ve OSI
değerleri klinik gebe olan ve olmayanlarda karşılaştırıldı.

Bulgular: Gebe olan ve olmayan
hastaların yaş, infertilite süresi, ovarian reserve ve transfer edilen embriyo
sayıları arasında istatistiksel bir fark yok idi. Ayrıca serum TAC,TOC ve OSI
değerleri arasında her üç fazda da (gonodatropın başlandığı gün, embriyo
transferi günü ve oosit toplandığı gün) ve folliküler sıvıda anlamlı bir fark
saptanmadı (p<0.05).

Sonuç: Yardımcı üreme
tekniklerine giden hastalarda TAC, TOC ve OSI değerleri klinik gebeliği
öngörmede etkisiz gözükmektedir.

Keywords

Folliküler sıvı, İn vitro fertilizasyon, Total oksidan kapasite, Total antioksidan kapasite

Assessment of serum and follicular fluid total oxidant and total antioxidant levels in In Vitro Fertilization (IVF) cases

Abstract

Objective: To assess the effects of serum and follicular
fluid total oxidant (TOC) levels, total antioxidant capacity (TAC), and
oxidative stress index (OSI) on oocyte maturation, fertilization,
embryogenesis, and clinical pregnancy outcomes in In Vitro Fertilization (IVF)
cycles of infertile patients.

Materials and Methods: One hundred patients having infertility
and underwent ART enrolled the study group and blood samples were collected on
gonadotropin starting, oocyte pick-up (OPU) and embryo transfer (ET) days.
Additionally, follicular fluid specimen obtained during OPU was collected. TOC,
TAC levels and OSI in serum samples and follicular fluid specimens between
clinically pregnant and non-pregnant patients were compared.

Results: No significant difference was noted
between clinically pregnant and nonpregnant patients in terms of the woman's
age, duration of infertility, ovarian reserve or number of transferred embryos.
There was also no significant difference in TAC, TOC and OSI levels in serum
samples obtained during three phases of treatment cycle (basal gonadotropin
starting day, OPU day and ET day) and in follicular fluid samples.

Conclusion: TAC, TOC and OSI seem to be ineffective
to predict clinical pregnancy as an outcome in patients who underwent ART.

Keywords

Follicular Fluid, Infertility, Total Oxidant Capacity, Total Antioxidant Capacity

References

• 1. Agarwal A, Saleh RA, Bedaiwy MA. Role of reactive oxygen species in the pathophysiology of human reproduction. Fertil Steril. 2003,79:829– 43.
• 2. Burton GJ, Jauniaux E: Oxidative Stress. Best Pract Res Clin Obstet Gynaecol 2010,25:287–299.
• 3. Agarwal A, Said TM, Bedaiwy MA, [et al.]. Oxidative stress in an assisted reproductive techniques setting. Fertil Steril. 2006,86:503-12.
• 4. Agarwal A, Aponte-Mellado A, Premkumar BJ, [et al.]. The effects of oxidative stress on female reproduction: a review. Reprod Biol Endocrinol. 2012,10-49.
• 5. Ko EY, Sabanegh ES Jr, Agarwal A. Male infertility testing: reactive oxygen species and antioxidant capacity. Fertil Steril. 2014,102:1518-27.
• 6. Ko EY, Sabanegh ES Jr, Agarwal A. Antioxidants for male subfertility. Fertil Steril. 2014,102:1518-27.
• 7. Roychoudhury S, Sharma R, Sikka S, [et al.]. Diagnostic application of total antioxidant capacity in seminal plasma to assess oxidative stress in male factor infertility. J Assist Reprod Genet. 2016, 33:627-35.
• 8. Bedaiwy MA, Mahfouz RZ, Goldberg JM, [et al.]. The effect of follicular fluid reactive oxygen species on the outcome of in vitro fertilization. Int J Fertil Womens Med 2000,45:314–320.
• 9. Das S, Chattopadhyay R, Ghosh S, [et al.]. Reactive oxygen species level in follicular fluid–embryo quality marker in IVF? Hum Reprod 2006, 21:2403–7.
• 10. Jana SK KNB, Chattopadhyay R, Chakravarty B, [et al.]. Upper control limit of reactive oxygen species in follicular fluid beyond which viable embryo formation is not favorable. Reprod Toxicol 2010, 29:447–451.
• 11. Pasqualotto EB, Agarwal A, Sharma RK, [et al.]. Effect of oxidative stress in follicular fluid on the outcome of assisted reproductive procedures. Fertil Steril 2004,81:973–6.
• 12. Erel O. A new automated colorimetric method for measuring total oxidant status. Clin Biochem. 2005,38:1103–11.
• 13. Erel O. A new automated method to measure total antioxidant response against potent free radical reactions. Clin Biochem. 2004,37:112–9.
• 14. Agarwal A, Gupta S, Sharma R. Oxidative stress and its implications in female infertility - a clinician's perspective. Reprod Biomed Online. 2005,11:641-50.
• 15. Oyawoye O, Abdel Gadir A, Garner A, [et al.]. Antioxidants and reactive oxygen species in follicular fluid of women undergoing IVF: relationship to outcome.Hum Reprod. 2003,18:2270–4.
• 16. Özatik O, Aydin Y, Hassa H, [et al.]. Relationship between oxidative stress and clinical pregnancy in assisted reproductive technology treatment cycles. J Assist Reprod Genet. 2013,30: 765-72.