Sağlıklı gebeliklerde fetal cinsiyet ile mikro RNA'ların ifade düzeyleri arasındaki ilişki

Year 2019, , 126 - 130, 15.09.2019

Selin Demirer Meryem Hocaoğlu , Bilge Özsait Selçuk , Abdulkadir Turgut , Evrim Kömürcü Bayrak

https://doi.org/10.16948/zktipb.529486

Abstract

SAĞLIKLI GEBELİKLERDE FETAL CİNSİYET İLE
MİKRORNA'LARIN İFADE DÜZEYLERİ ARASINDAKİ İLİŞKİ

Özet

Amaç:
Sağlıklı gebelikte maternal kan ve plasentaya özgü
miRNA’ların araştırılması, maternal/fetal biyolojik ve fizyolojik süreçlerin
anlaşılmasına yardımcı olmaktadır. Fetüste dişi ve erkek cinsiyet
arasındaki hormonal ve genetik farklılıkların sonucu olarak miRNA ifade
düzeyleri değişmektedir. Bu çalışmada amacımız, aday olarak belirlediğimiz
miRNA-21-3p, miRNA-155-5p, miRNA-518b ve miR-16-5p ifade düzeylerinin sağlıklı
gebelerde fetal cinsiyet ile ilişkisinin araştırılmasıdır.

Gereçler
ve Yöntem: Çalışma grubu, Kasım 2017 – Mart 2018 tarihlerinde
İstanbul Medeniyet Üniversitesi Göztepe Eğitim ve Araştırma Hastanesi Kadın
Hastalıkları ve Doğum Kliniğinde gebeliği takip edilen, maternal ve/veya fetal
hastalık saptanmayan sağlıklı 21 gebeden oluşmaktadır. Maternal kan örnekleri
aynı gebelerin 29. (Grup 1) ve 37. gebelik (Grup 2) haftalarındaki takiplerinde
alınmıştır. Maternal kan
lökositlerinden RNA izolasyonunun ardından miR-21-3p, miR-155-5p, miR-518b ve
miR-16-5p anlatım düzeyleri, SYBR-Green gerçek zamanlı kantitatif PCR ile
belirlenmiştir. Gruplar ve fetal cinsiyetler arasındaki miRNA ifade düzeyleri
istatistiksel olarak karşılaştırılmıştır.

Bulgular:
Grup
1 ve Grup 2’de fetal cinsiyet ile klinik ve biyokimyasal parametreler arasında
istatistiksel olarak anlamlı bir fark bulunmasa da  (p>0,05) miRNA ifade düzeyleri ilişkili
bulunmuştur. Buna göre, kız fetüs taşıyan gebelerde erkek fetüs taşıyanlara
oranla 29. haftada miR-16-5p (p=0,01) ifade düzeyinin artmış olduğu
belirlenmiştir. Erkek fetüs taşıyan gebelerde ise kız fetüs taşıyanlara oranla
37. haftada miR-21-3p (p=0,02), miR-155-5p (p=0,08) ve miR-518b (p=0,02) ifade
düzeylerinin artmış olduğu saptanmıştır.

Sonuç: İlk
defa bu çalışmada, sağlıklı gebelikte maternal kandaki lökositlerde üçüncü trimesterin
başında ve sonunda fetal cinsiyet ile değişen miRNA ifade düzeylerinin olduğu
gösterilmiştir.

Keywords

Gebelik, fetüs, miRNA, lökositler, cinsiyet

RELATIONSHIP BETWEEN FETAL SEX AND THE EXPRESSION LEVELS OF MICRORNAS IN HEALTHY PREGNANCIES

Abstract

RELATIONSHIP BETWEEN FETAL SEX AND
THE EXPRESSION LEVELS OF MICRORNAS IN HEALTHY PREGNANCIES

Objective:
Differences in
microRNA (miRNA) expression in maternal blood and placenta can help us further
understand maternal and fetal biology and physiology. Fetal sex differences in
miRNA expression are a result of both hormonal and genetic differences between
the sexes. The aim of this study was to evaluate the relationship between the
expression levels of miRNA-21-3p, miRNA-155-5p, miRNA-518b and miR-16-5p and
fetal sex.

Materials
and Methods: This study was carried out at the Department of
Obstetrics and Gynecology of Istanbul Medeniyet University, Goztepe Research
and Training Hospital. Twenty-one
healthy pregnant women, who were having their pregnancy care through outpatient
setting between November 2017 and March 2018, were included in the study.
Maternal peripheral blood samples were obtained from the same healthy pregnant
females at 29 weeks of gestation (Group 1) and at 37 weeks of gestation (Group
2). The maternal blood leucocyte levels of miRNAs (miRNA-21-3p, miRNA-155-5p, miRNA-518b and miR-16-5p were analyzed
using SYBR-Green real-time quantitative polymerase chain reaction. The
expression levels of miRNAs between groups and fetal sexes were analyzed
statistically.

Results: There were no significant differences in clinical and
laboratory characteristics between fetal sex in Group 1 and Group 2 (p>
0,05). There was a significant increase in the expression levels of miR-16-5p
(p=0,01) in pregnant women with female offspring, compared to the pregnant with
male offspring at 29 weeks of gestation. There were significant increases in
the expression levels of miR-21-3p (p=0,02), miR-155-5p (p=0,08), miR-518b
(p=0,02) in pregnant women with male offspring, compared to the pregnant with
female offspring at 37 weeks of gestation.

Conclusion: For the first time, in this study were shown to have
differential expression levels of maternal blood leukocyte miRNAs between the
fetal sexes at the beginning and end of the third trimester.

Keywords

Pregnancy, fetus, miRNA, leukocyte, sex

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