Abstract
Purpose: Expression of Luteinizing hormone (LH) receptors has been showed in the tuba uterina; but their role in the Fallopian tube is still unknown. During the normal menstrual cycle, female reproductive system is exposed to fluctuating levels of sex steroids, including estrogen and progesterone. In this study, we present the cyclical variations of tubal LH, estro-gen receptors (ER), and progesterone receptors (PR).Material and Methods: Twenty women operated for benign causes were included in this study. Fallopian tubes from the different stages of menstrual cycle were collected. Then these tubes sections were examined using immunohistochemistry.Results: Expression of LH receptor did not change according to phases of menstrual cycle and there is no significant difference between follicular phase and luteal phase groups. Ac-cording to the result of statistically analyzed, there is not a significantly differences between the PR of follicular phase and luteal phase.Conclusion: We report on differences in LHR, SHR expression in the Fallopian tube from cases with follicular phase compared with luteal phase. This information may be important for the understanding of normal tubal function.
References
- Gawronska B, Paukku T, Huntaniemi I, Wasowicz G, Ziecik AJ. Oestrogen-dependent expression of LH/hCG receptors in pig Fallopian tube and their role in relaxation of the oviduct. J Reprod Fertil 1999; 115:293-301.
- Pierce JG, Parsons TF. Glycoprotein hormones: Structure and function. Annu Rev Biochem 1981; 50:466-495.
- Mc Farland KC, Sprengel R, Philips HS, Kohler M, Rosemblit N, Nicolics K, Segaloff DL, Seeborg PH. Lutropin-choriogonadotropin receptor: an unusul member of the G-protein-coupled receptor family. Science 1989; 245:494-499.
- Loosfelt H, Misrahi M, Atger M, Salesse R, Thi MTVH-L, Jolivet A, GuichonMantel A, Sar S, Jallal B, Garnier J, Migrom E. Cloning and sequencing of porcine LH/hCG receptor cDNA: variants lacking transmembrane domain. Science 1989; 245:525– 528
- Lei ZM, Toth P, Rao ChV, Pridham D. Novel co-expression of humanchorionic gonadotropin (hCG)/human luteinizing hormone receptors and their ligand, hCG, in human fallopian tubes. J Clin Endocrinol Metab 1993; 77:863–872
- Bhattacharya S, Banerjee J, Sen S, Manna PR. Human chorionic gonadotropin binding sites in the human endometrium. Acta Endocrinol 1993; 129:15–19
- Bernardini L, Moretti-Rojas I, Brush M, Rojas FJ, Balmaceda JP. Statusof hCG/LH receptor and G-proteins in human endometrium during artificial cycles of hormone replacement therapy. J Soc Gynecol Invest 1995; 2:630–635
- Rao ChV. An overview of the past, present and future of nongonadal LH/hCG actions in reproductive biology and medicine. Semin Reprod Med 2001; 19:7-17
- Derecka K, Pietilla EM, Rajaniemi HJ, Ziecik AJ. Cycle dependent LH/hCG receptor gene expression in porcine nongonadal reproductive tissues. J Physiol Pharmacol 1995; 46:77-85
- Ekerhovd E, Brannström M, Alexanderson M, Norstrom A. Evidence for nitric oxide mediation of contractile activity in isolated strips of the human Fallopian tube. Human Reprod 1997; 12:301-305
- Flowers B, Ziecik AJ, Carolo EV. Effects of human chorionic gonadotrophin on contractile activity of steroid-primed pig myometrium in vitro. J Reprod Fertil 1991; 92:425-432
- Mangelsdorf DJ, Thummel C, Beato M, herrlich P, Schütz G, Umesono K, Blumberg B, Kastner P, Mark M, Chambon P, Evans RM. The nuclear receptor superfamily: the second decade. Cell 1995; 83:835-839.
- Amso NN, Crow J, Shaw RW. Comparative immunohistochemical study of oestrogen and progesterone receptors in the Fallopian tube and uterus at different stages of menstruel cycle and the menopouse. Hum Reprod 1994; 9:1027-1037
- Coppens MT, de Boever JG, Dhont MA, Serreyn RF, Vandekerckhove DA, Roels HJ. Topographical distribution of oestrogen and progesterone receptors in the human endometrium and Fallopian tube. An immunohistochemical study. Histochemistry 1994; 99:127-131
- Gawronska B, Stepien A, Ziecik AJ. Effect of estradiol and progesterone on oviductal LH-dependent relaxation of the porcine oviduct. Theriogenology 2000; 53:659-672.
- Verhage HG, Bareither ML, Jaffe RC, Akbar M. Cyclic changes in ciliation, secretion and cell height of the oviductal epithelium in women. Am J Anat 1979; 156:505-521
- Wanggren K, Stavreus-Evers A, Olsson C, Anderson E, Gemzell-Danielsson K. Regulation of muscular contractions in the human Fallopian tube through prostaglandins and progestagens. 2008; 23: 2359-2368
- Lindblom B, Wilhelmsson L, Wikland M, Hamberger L, Wiqvist N. Prostoglandins and oviductal function. Acta Obstet Gynecol Scand Suppl 1983; 113:43-46
- Jansen RP, Fallopian tube istmic mucus and ovum transport. Science 1978; 201:349-351
- Blandau RJ, Gaddum-Rosse P. Mechanism of sperm transport in pig oviducts. Fertil Steril 1974; 25:61-67.
Menstruel Siklus Sırasında Fallop Tüplerinde Lüteinizan Hormon ve Seks Steroid Hormon Reseptörlerinin Ekspresyonu (The Expressions of Luteinizing Hormone and Sex Steroid Hormone Receptors in Human Fallopian Tube During the Menstrual Cycle)
Abstract
Amaç: Luteinizan hormon reseptörleri daha önce tubada gösterilmekle birlikte henüz buradaki rolleri tam olarak bilinmemektedir. Menstruel siklus sırasında kadın genital sistemi östrojen ve progesteronu içeren seks steroidlerinin dalgalanan düzeylerine maruz kalırlar. Biz bu çalışmada Luteinizan hormon, östrojen ve progesteron reseptörlerinin tubada siklik varyasyonlarını araştırdık.
Materyal ve Metod: Bu çalışmaya benign nedenlerle opere olan 20 olgu dahil edildi. Menstruel siklusun farklı fazlarına sahip olguların Fallopian tüpleri seçildi. Daha sonra bu tüplere immunohistokimyasal boyama uygulandı.
Bulgular: Lüteinizan hormon reseptörlerinin ekspresyonları menstruel siklusun farklı fazlarına göre değişiklik göstermemekteydi. İstatiksel analiz sonuçlarına göre östrojen ve progesteron reseptörü ekspresyonunda fazlar arasında fark izlenmedi. Ama progesteron reseptör boyanma yoğunluğu belirgin olarak lueal fazda folliküler faza göre daha yüksekti.
Sonuç: Bu çalışmada Fallop tübündeki Lüteinizan hormon, östrojen ve progesteron reseptör ekspresyonlarının folliküler faz ile luteal faz arasındaki farklılıklarını araştırdık. Bu bilgilerin normal tüp fonksiyonlarını anlamada önemli olduğunu düşünmekteyiz.
Anahtar kelimeler: Lüteinizan hormon, fallop tüpü, immunohistokimya
ABSTRACT
Purpose: Expression of Luteinizing hormone (LH) receptors has been showed in the tuba uterina; but their role in the Fallopian tube is still unknown. During the normal menstrual cycle, female reproductive system is exposed to fluctuating levels of sex steroids, including estrogen and progesterone. In this study, we present the cyclical variations of tubal LH, estro-gen receptors (ER), and progesterone receptors (PR).
Material and Methods: Twenty women operated for benign causes were included in this study. Fallopian tubes from the different stages of menstrual cycle were collected. Then these tubes sections were examined using immunohistochemistry.
Results: Expression of LH receptor did not change according to phases of menstrual cycle and there is no significant difference between follicular phase and luteal phase groups. Ac-cording to the result of statistically analyzed, there is not a significantly differences between the PR of follicular phase and luteal phase.
Conclusion: We report on differences in LHR, SHR expression in the Fallopian tube from cases with follicular phase compared with luteal phase. This information may be important for the understanding of normal tubal function.
Key words: Luteinizing hormone, Fallopian tube, immunohistochemistry
Keywords
References
- Gawronska B, Paukku T, Huntaniemi I, Wasowicz G, Ziecik AJ. Oestrogen-dependent expression of LH/hCG receptors in pig Fallopian tube and their role in relaxation of the oviduct. J Reprod Fertil 1999; 115:293-301.
- Pierce JG, Parsons TF. Glycoprotein hormones: Structure and function. Annu Rev Biochem 1981; 50:466-495.
- Mc Farland KC, Sprengel R, Philips HS, Kohler M, Rosemblit N, Nicolics K, Segaloff DL, Seeborg PH. Lutropin-choriogonadotropin receptor: an unusul member of the G-protein-coupled receptor family. Science 1989; 245:494-499.
- Loosfelt H, Misrahi M, Atger M, Salesse R, Thi MTVH-L, Jolivet A, GuichonMantel A, Sar S, Jallal B, Garnier J, Migrom E. Cloning and sequencing of porcine LH/hCG receptor cDNA: variants lacking transmembrane domain. Science 1989; 245:525– 528
- Lei ZM, Toth P, Rao ChV, Pridham D. Novel co-expression of humanchorionic gonadotropin (hCG)/human luteinizing hormone receptors and their ligand, hCG, in human fallopian tubes. J Clin Endocrinol Metab 1993; 77:863–872
- Bhattacharya S, Banerjee J, Sen S, Manna PR. Human chorionic gonadotropin binding sites in the human endometrium. Acta Endocrinol 1993; 129:15–19
- Bernardini L, Moretti-Rojas I, Brush M, Rojas FJ, Balmaceda JP. Statusof hCG/LH receptor and G-proteins in human endometrium during artificial cycles of hormone replacement therapy. J Soc Gynecol Invest 1995; 2:630–635
- Rao ChV. An overview of the past, present and future of nongonadal LH/hCG actions in reproductive biology and medicine. Semin Reprod Med 2001; 19:7-17
- Derecka K, Pietilla EM, Rajaniemi HJ, Ziecik AJ. Cycle dependent LH/hCG receptor gene expression in porcine nongonadal reproductive tissues. J Physiol Pharmacol 1995; 46:77-85
- Ekerhovd E, Brannström M, Alexanderson M, Norstrom A. Evidence for nitric oxide mediation of contractile activity in isolated strips of the human Fallopian tube. Human Reprod 1997; 12:301-305
- Flowers B, Ziecik AJ, Carolo EV. Effects of human chorionic gonadotrophin on contractile activity of steroid-primed pig myometrium in vitro. J Reprod Fertil 1991; 92:425-432
- Mangelsdorf DJ, Thummel C, Beato M, herrlich P, Schütz G, Umesono K, Blumberg B, Kastner P, Mark M, Chambon P, Evans RM. The nuclear receptor superfamily: the second decade. Cell 1995; 83:835-839.
- Amso NN, Crow J, Shaw RW. Comparative immunohistochemical study of oestrogen and progesterone receptors in the Fallopian tube and uterus at different stages of menstruel cycle and the menopouse. Hum Reprod 1994; 9:1027-1037
- Coppens MT, de Boever JG, Dhont MA, Serreyn RF, Vandekerckhove DA, Roels HJ. Topographical distribution of oestrogen and progesterone receptors in the human endometrium and Fallopian tube. An immunohistochemical study. Histochemistry 1994; 99:127-131
- Gawronska B, Stepien A, Ziecik AJ. Effect of estradiol and progesterone on oviductal LH-dependent relaxation of the porcine oviduct. Theriogenology 2000; 53:659-672.
- Verhage HG, Bareither ML, Jaffe RC, Akbar M. Cyclic changes in ciliation, secretion and cell height of the oviductal epithelium in women. Am J Anat 1979; 156:505-521
- Wanggren K, Stavreus-Evers A, Olsson C, Anderson E, Gemzell-Danielsson K. Regulation of muscular contractions in the human Fallopian tube through prostaglandins and progestagens. 2008; 23: 2359-2368
- Lindblom B, Wilhelmsson L, Wikland M, Hamberger L, Wiqvist N. Prostoglandins and oviductal function. Acta Obstet Gynecol Scand Suppl 1983; 113:43-46
- Jansen RP, Fallopian tube istmic mucus and ovum transport. Science 1978; 201:349-351
- Blandau RJ, Gaddum-Rosse P. Mechanism of sperm transport in pig oviducts. Fertil Steril 1974; 25:61-67.
Details
| Primary Language | Turkish |
|---|---|
| Journal Section | Original Research |
| Authors | |
| Publication Date | August 6, 2014 |
| Published in Issue | Year 2014 Volume: 45 Issue: 3 |
